Patent application number | Description | Published |
20090149631 | Methods and compositions for protein labeling using lipoic acid ligases - The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs recognized by lipoic acid ligase and lipoic acid ligase mutants. | 06-11-2009 |
20110130348 | KINETICALLY EFFICIENT SUBSTRATE FOR LIPOIC ACID LIGASE - The invention provides methods for identifying and optimizing peptide substrates for enzymes such as lipoic acid ligase (Lp1A). | 06-02-2011 |
20120129159 | METHODS AND COMPOSITIONS FOR PROTEIN LABELING USING LIPOIC ACID LIGASES - The present disclosure provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs (e.g., lipoic acid analogs comprising a resorufin moiety) recognized by lipoic acid ligase and lipoic acid ligase mutants. Also provided herein is a method of imaging protein-protein interaction via a reaction mediated by lipoic acid ligase. | 05-24-2012 |
20120214201 | PROBE INCORPORATION MEDIATED BY ENZYMES - The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs recognized by lipoic acid ligase and lipoic acid ligase mutants. | 08-23-2012 |
20140186870 | IN VIVO PROTEOMICS - The application discloses methods, materials, and compositions for the labeling of molecules, for example, proteins, in living cells or in subcellular compartments of living cells. In particular, the application relates to proteomic analysis methods; materials and compositions and means based on direct tagging of unknown proteins with tagging enzymes (such as biotin ligase or a peroxidase) within the vicinity of a tagging substrate (such as a tyramide) within living cells, with optional targeting to specific subcellular locations by expression of genetic constructs. | 07-03-2014 |
20140206013 | SPLIT PEROXIDASES AND METHODS OF USE - An imaging method utilizing a split peroxidase is described herein. Imaging methods involve contacting a cell with a split peroxidase and a substrate thereof to allow conversion of a substrate into a product via an enzymatic reaction catalyzed by the reconstitute split peroxidase. Also disclosed herein are split peroxidases, related products and kits. | 07-24-2014 |
20150037829 | CYTOSOLICALLY-ACTIVE PEROXIDASES AS REPORTERS FOR MICROSCOPY - An imaging method comprising expressing in cells a Class I heme peroxidase, which optionally is fused with a protein of interest or a cellular localization signal peptide, and contacting the cells with a substrate of the Class I heme peroxidase to allow conversion of the substrate into a product via an oxidation reaction catalyzed by the Class I heme peroxidase, wherein the product releases a signal detectable by a microscope such as an electron microscope. Also disclosed herein are monomeric mutants of a Class I heme peroxidase and mutants of the enzyme that exhibit elevated enzymatic activity as compared to the corresponding wild-type counterpart. | 02-05-2015 |
20150125904 | PROBE INCORPORATION MEDIATED BY ENZYMES - Compositions (e.g., lipoic acid ligase polypeptides and lipoic acid analogs) and uses thereof in the Probe Incorporation Mediated By Enzymes (PRIME) methods both in vitro and in vivo. Also described herein are kits for performing the PRIME method and vectors/kits for expressing the lipoic acid ligases. | 05-07-2015 |