Patent application number | Description | Published |
20080293127 | Method to Render Pressure Sensitive Adhesive Compatible with Polymerase Chain Reaction Systems - A method for substantially eliminating a cross-linking element of a pressure sensitive adhesive by heating the pressure sensitive adhesive at a temperature of at least 180° C. for a period of time required to thermally decompose the cross-linking element of the pressure sensitive adhesive. | 11-27-2008 |
20090023189 | APPARATUS AND METHODS FOR PREPARATION OF SUBTANTIALLY UNIFORM EMULSIONS CONTAINING A PARTICLE - Methods and systems for forming water-in-oil emulsions are described. For example, an apparatus is described which includes: a first compartment containing a plurality of particles dispersed in an aqueous phase; a second compartment containing an oil phase; a porous layer separating the first and second compartments; and a device for applying pressure to the first compartment. A method is described which includes: moving an oil phase relative to a surface of a porous layer while simultaneously forcing an aqueous composition comprising particles through the porous layer and into the flowing dispersion medium thereby forming droplets of the aqueous composition containing particles dispersed in the oil phase. The aqueous composition can include one or more nucleic acid templates and reagents for amplifying the nucleic acids such as PCR reagents. A porous partition is described comprising a first and second major surfaces and at least two straight through pores comprising a cross sectional shape selected from a polygon, an oval, an oblong, a dumbbell, a bowtie and irregular shapes thereof. Aqueous droplets containing an oligonucleotide attached to a particle and reagents can be used as a microreactor for nucleic acid amplification. | 01-22-2009 |
20090081768 | Devices and Methods for Thermally Isolating Chambers of an Assay Card - An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card. | 03-26-2009 |
20090142772 | Devices and Methods for the Detection of Analytes - System and methods for detecting analytes such as pathogenic cells are described. The methods allow for the direct measurement of analytes such as pathogenic organisms without the need for sample preparation and/or PCR. The devices can be used individually as point-of-use sensors for airborne pathogens and other pathogenic organisms in foods and agriculture products. | 06-04-2009 |
20090233327 | Surface Modification in a Manipulation Chamber - A device for manipulating biological material, the device including at least one electrode and a photoconductive material configured to receive the biological material; and a light source configured to illuminate the photoconductive material so as to modulate an electric field, wherein the electric field is configured to manipulate the biological material; wherein a surface of the at least one electrode and/or the photoconductive material is modified with at least one of a carboxylic moiety, an amino moiety, a poly(ethylene glycol) moiety, a polymer of (poly(ethylene oxide) methyl ether) acrylate, a poly(2-hydroxyethyl (meth)acrylate), a poly(N-vinylpyrrolidone), a poly(N-vinylformamide), a poly(N-vinylformamide) derivative, a poly((meth)acrylamide), and a poly((meth)acrylamide) derivative. Methods for manipulating biological material are also disclosed. | 09-17-2009 |
20100051461 | Poly and Copolyn(N-Vinylamide)s and their use in Capillary Electrophoresis - The invention relates generally to polymers and copolymers comprising N-vinylamide-type monomers, their preparation, and compositions, such as electrophoresis separation media, containing the same; to supports, such as capillaries, containing these polymers; and methods for separating a mixture of biomolecules, especially polynucleotides, using capillary electrophoresis. Separation media comprising such polymers yield advantageous performance in the analysis and separation of biomolecules by capillary electrophoresis. | 03-04-2010 |
20100092867 | Porous Polymer Electrodes - Porous polymer electrode assemblies are useful in the detection or quantification of a variety of analytes. By preparing a porous monolith, and applying a conductive polymer to the monolith, a porous matrix is prepared that combines favorable conductive properties, by virtue of the presence of the conductive polymer, with the porous character of the underlying monolith. The resulting porous electrode can be used for qualitative or quantitative analysis, and the capture and/or release of selected charged materials, such as nucleic acids. The pores of the electrode matrix may also be filled with nonconductive material, yielding electrodes having a plurality of discrete conductive surfaces. | 04-15-2010 |
20100105040 | MICROFLUIDIC SYSTEMS INCLUDING POROUS POLYMER ELECTRODES - Microfluidic devices that incorporate a porous polymer electrode assemblies, including microfluidic device useful for detection of nucleic acids, as well as methods of using the microfluidic devices. | 04-29-2010 |
20100133105 | Optoelectronic Separation of Biomolecules - The present teachings relate to systems and methods for separation of substances such as cells, nucleic acids, and carbon nanotubes. The substances are combined with a separation medium in a liquid sample cavity, for example a microchannel, and transit through the separation by optically activated dielectrophoretic forces. The substances are advantageously labeled and visualized using a microscope and camera. | 06-03-2010 |
20100179075 | PARTICLES FOR USE IN SUPPORTED NUCLEIC ACID LIGATION AND DETECTION SEQUENCING - Compositions and methods to modify the surface of particles to which biomolecules are attached are disclosed. The particles can include beads and nanoparticles which are composed of metallics, metal alloys, glass, polymers and derivatives and composites thereof. The surface of the particles are modified to be hydrophilic for ease in the attachment of biomolecules to the particle surface and immobilization of the particles to a substrate to facilitate process such as nucleic acid sequencing, PCR and sequencing by ligation. | 07-15-2010 |
20100187111 | Graft Copolymers, Their Preparation And Use in Capillary Electrophoresis - The invention relates to graft copolymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; also to ultra-high molecular weight poly(N,N-dimethylacrylamide) (“poly(DMA)”) polymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; and more particularly to supports, such as capillaries, containing these polymers and methods for separating biomolecules, especially polynucleotides, using capillary electrophoresis. The graft copolymers can be prepared by, e.g., grafting polyacrylamide units onto a poly(DMA) backbone. Separation media comprising such graft copolymers or ultra-high molecular weight poly(DMA) polymers yield superior performance in the analysis and separation of biomolecules by capillary electrophoresis. | 07-29-2010 |
20100196912 | Polyelectrolyte-Coated Size-Exclusion Ion-Exchange Particles - A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided. | 08-05-2010 |
20100206731 | DEVICES AND METHODS FOR OPTOELECTRONIC MANIPULATION OF SMALL PARTICLES - A method for sorting cells in a biological sample comprising a first type of cells and a second type of cells may comprise introducing the biological sample into a chamber comprising a first surface and a second surface, wherein the first surface is associated with a transparent electrode and the second surface is associated with a photoconductive portion of an electrode. The method may further comprise moving incident light and the photoconductive portion relative to one another so as to illuminate regions of the photoconductive portion and modulate an electric field in the chamber in proximity to the illuminated regions. The method may further comprise separating the first type of cells from the second type of cells in the chamber via dielectrophoretic movement of the first type of cells and the second type of cells caused by the modulated electric field, wherein a dielectrophoretic characteristic of at least one of the first type of cells and the second type of cells has been modified. | 08-19-2010 |
20100317841 | Polyelectrolyte-Coated Size-Exclusion Ion-Exchange Particles - A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided. | 12-16-2010 |
20110085168 | Luminescence Reference Standards - The present teachings provide for systems, and components thereof, for detecting and/or analyzing light. These systems can include, among others, optical reference standards utilizing luminophores, such as nanocrystals, for calibrating, validating, and/or monitoring light-detection systems, before, during, and/or after sample analysis. | 04-14-2011 |
20110114206 | Fluid Processing Device Including Size-Changing Barrier - A diagnostic device is provided that includes a plurality of retainment regions interconnected through at least one fluid processing passageway or separated by at least one barrier. A fluid flow modulator can be provided in the fluid processing passageway if a fluid processing passageway is provided. The barrier and/or fluid flow modulator can comprise a polysaccharide, a derivative of a polysaccharide, or a combination thereof. For example, the barrier can comprise a chitosan material. | 05-19-2011 |
20110236984 | DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME - In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand. | 09-29-2011 |
20120214156 | DEVICE INCLUDING A DISSOLVABLE STRUCTURE FOR FLOW CONTROL - A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway. | 08-23-2012 |
20120292244 | POLYELECTROLYTE-COATED SIZE-EXCLUSION ION-EXCHANGE PARTICLES - A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided. | 11-22-2012 |
20120305838 | Polyelectrolyte-Coated Size-Exclusion Ion-Exchange Particles - A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided. | 12-06-2012 |
20130105315 | HIGH SPEED, HIGH RESOLUTION COMPOSITIONS, METHODS AND KITS FOR CAPILLARY ELECTROPHORESIS | 05-02-2013 |
20130153421 | GRAFT COPOLYMERS, THEIR PREPARATION AND USE IN CAPILLARY ELECTROPHORESIS - The invention relates to graft copolymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; also to ultra-high molecular weight poly(N,N-dimethylacrylamide) (“poly(DMA)”) polymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; and more particularly to supports, such as capillaries, containing these polymers and methods for separating biomolecules, especially polynucleotides, using capillary electrophoresis. The graft copolymers can be prepared by, e.g., grafting polyacrylamide units onto a poly(DMA) backbone. Separation media comprising such graft copolymers or ultra-high molecular weight poly(DMA) polymers yield superior performance in the analysis and separation of biomolecules by capillary electrophoresis. | 06-20-2013 |
20130164748 | Detection of nucleic acid amplification - Methods for detecting a target polynucleotide sequences are provided that utilize a probe having a target-complementary segment and a detectable tag. By cleaving the detectable tab and associating the tag with a tag complement coupled to an electrode, an electrochemical signal can be detected that is related to the presence of the tag:tag complement complex. | 06-27-2013 |
20140090980 | High Speed, High Resolution Compositions, Methods and Kits for Capillary Electrophoresis - The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided. | 04-03-2014 |
20140209461 | Poly and Copoly(N-vinylamide)s and Their Use In Capillary Electrophoresis - The invention relates generally to polymers and copolymers comprising N-vinylamide-type monomers, their preparation, and compositions, such as electrophoresis separation media, containing the same; to supports, such as capillaries, containing these polymers; and methods for separating a mixture of biomolecules, especially polynucleotides, using capillary electrophoresis. Separation media comprising such polymers yield advantageous performance in the analysis and separation of biomolecules by capillary electrophoresis. | 07-31-2014 |
20150015876 | Luminescence Reference Standards - The present teachings provide for systems, and components thereof, for detecting and/or analyzing light. These systems can include, among others, optical reference standards utilizing luminophores, such as nanocrystals, for calibrating, validating, and/or monitoring light-detection systems, before, during, and/or after sample analysis. | 01-15-2015 |