45th week of 2013 patent applcation highlights part 44 |
Patent application number | Title | Published |
20130295575 | MICROPLATE AND MULTIWELL STRIP WITH DOUBLE RIMMED WELLS - The application discloses a microplate, wherein the openings of the wells for receiving reagents have a collar, wherein the collar comprises at least two upwardly extending rims, wherein the rims are radially separated by a gap ( | 2013-11-07 |
20130295576 | METHOD FOR MODIFYING NUCLEIC ACIDS - The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods. | 2013-11-07 |
20130295577 | METHOD AND KIT FOR THE QUANTIFICATION OF NUCLEIC ACIDS - Disclosed are a method and a kit for the quantification of nucleic acids, especially a trace amount of nucleic acid, such as host cell nucleic acid impurities, using real-time PCR with a random primer. | 2013-11-07 |
20130295578 | METHODS FOR SCREENING FOR DRUG RESISTANCE IN CANCER TREATMENT - The present invention includes methods for screening for drug resistance in cancer tissue ex vivo which comprises a novel 3-dimensional cell culture system that mimics the tumor microenvironment. | 2013-11-07 |
20130295579 | METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELLS AND MEDIUM USED FOR PREPARING INDUCED PLURIPOTENT STEM CELLS - A method for preparing induced pluripotent stem (iPS) cells, which comprises steps as follows: step 1, introducing one or more stem cell pluripotency factors into somatic cells; step 2, culturing the somatic cells, into which the stem cell pluripotency factor has been introduced in the Step 1, by using medium supplemented with lithium salt; and step 3, identifying and characterizing the induced pluripotent stem cells. Furthermore, there provided a medium for preparing induced pluripotent stem cells, which comprising lithium salt. The medium supplemented with lithium salt is used for efficiently inducing pluripotent stem cells. Lithium salt is able to increase the production efficiency of mouse iPS cells by 5-60 times. The present method for inducing iPS cells is in favor of improving the safety of iPS technique and application of iPS cells in regenerative medicine. | 2013-11-07 |
20130295580 | ORAL CANCER POINT OF CARE DIAGNOSTICS - A point of care diagnostic test, device and disposables for determining a patient risk for oral cancer in the same visit that a sample is collected. | 2013-11-07 |
20130295581 | Methods and Compositions for the Treatment and Diagnosis of Breast Cancer - The invention provides methods, compositions and kits relating to the diagnosis, prognosis and treatment of breast cancer. | 2013-11-07 |
20130295582 | PAN-KINASE ACTIVATION AND EVALUATION OF SIGNALING PATHWAYS - Methods and reagents are provided for determining the activation state of a signal transduction pathway signaling protein. There exists a need in the art for methods that can monitor the efficacy of a signal transduction inhibitor in a patient. Other needs exist for detecting and monitoring certain disease or disorders that are associated with aberrant activation of a signal transduction pathway signaling protein. The present assay provides a highly sensitive assay that is also useful in patient populations in which obtaining a large cellular sample is difficult, for example, neonates. | 2013-11-07 |
20130295583 | Method to Increase Specificity and/or Accuracy of Lateral Flow Immunoassays - The present invention includes methods and devices for preventing interfering substances from affecting the accuracy of a lateral flow immunoassay. In preferred embodiments, a test strip includes a capturing zone that includes at least one mobile capturing reagent that separates at least one interfering substance from the analyte. The capturing zone is preferably located upstream of the sample application zone. In some embodiments, the reagent/conjugate zone is also located upstream of the sample application zone. The capturing zone may be located upstream, downstream, or overlapping with the reagent/conjugate zone in these embodiments. In other preferred embodiments, one or more mobile capturing reagents are included in the elution medium/running buffer. In yet other embodiments, the capturing reagent is incorporated into a sample collection device of a sample collection system, preferably separate from the chromatographic test strip. A lysis zone is also included in some preferred embodiments. | 2013-11-07 |
20130295584 | HISTONE PROTEIN UBIQUITINATION AS A CANCER BIOMARKER - The present disclosure relates generally to the field of cancer diagnosis. More specifically, the present disclosure relates to the identification and use of monoubiquitination of histone 2B as a biomarker for the diagnosis and prognosis of cancer including, but not limited to, parathyroid cancer. The present disclosure also relates to the identification of binding between CDC73 and RNF20, and the use of CDC73 and RNF20 in an assay for screening for an agent that modulates monoubiquitination of a histone protein. | 2013-11-07 |
20130295585 | STIMULI-RESPONSIVE POLYMER DIAGNOSTIC ASSAY COMPRISING MAGNETIC NANOPARTICLES AND CAPTURE CONJUGATES - The present disclosure utilizes the aggregation of stimuli-responsive polymers to isolate a diagnostic target (e.g., an antigen) from a solution using magnetophoresis. Isolating the diagnostic target provides a route to identify the presence of the diagnostic target in the solution. | 2013-11-07 |
20130295586 | PREPARATION OF NANOCRYSTALS WITH MIXTURES OF ORGANIC LIGANDS - Semiconductor nanocrystals prepared using a mixture of organic ligands (e.g., oxoacids), as well as compositions, kits, and methods of using such semiconductor nanocrystals are disclosed. | 2013-11-07 |
20130295587 | METHOD OF COUPLING BINDING AGENTS TO A SUBSTRATE SURFACE - A chemical modification method of a defined area on a substrate surface, comprising the steps of:
| 2013-11-07 |
20130295588 | COUNTING PARTICLES USING AN ELECTRICAL DIFFERENTIAL COUNTER - This disclosure relates to methods and devices to count particles of interest, such as cells. The methods include obtaining a fluid sample that may contain particles of interest; counting all types of particles in a portion of the sample using a first electrical differential counter to generate a first total; removing any particles of interest from the portion of the fluid sample; counting any particles remaining in the portion of the fluid sample using a second electrical differential counter after the particles of interest are removed to generate a second total; and calculating a number of particles of interest originally in the fluid sample by subtracting the second total from the first total, wherein the difference is the number of particles of interest in the sample. These methods and related devices can be used, for example, to produce a robust, inexpensive diagnostic kit for CD4+ T cell counting in whole blood samples. | 2013-11-07 |
20130295589 | NGAL FOR DIAGNOSIS OF RENAL CONDITIONS - Use of serum neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker, alone or in conjunction with creatinine to aid in the diagnosis of renal conditions such as acute tubular necrosis and acute renal failure, and a method and a kit for assigning a diagnosis of acute tubular necrosis or acute renal failure to a subject based on the correlation between the levels of NGAL and optionally creatinine in a sample obtained from a subject when compared to a sample obtained from a normal subject not experiencing acute tubular necrosis or acute renal failure. | 2013-11-07 |
20130295590 | FOXA1 AS A MARKER FOR INVASIVE BLADDER CANCER - The present invention provides for a proteomic approach to predicting, diagnosing and staging invasive bladder cancer, and for predicting patient survival and therapeutic efficacy. More specifically, the target being analyzed for reduced expression is FOXA1, and optionally including analysis of increased FOXA2 expression. | 2013-11-07 |
20130295591 | SYSTEMS FOR IMMUNOASSAY TESTS - This invention relates to a cartridge for an immunoassay test. The cartridge comprises (a) a probe well comprising a probe and a cap, the cap being in a closed position to enclose the probe in the probe well, wherein the probe has a bottom tip coated with analyte-binding molecules; (b) a sample well to receive a sample; (c) one or more reagent wells; (d) a plurality of wash wells each containing a first aqueous solution; and (e) a measurement well having a light transmissive bottom, the measurement well containing a second aqueous solution; wherein the openings of the sample well, reagent well, measurement well and wash wells are sealed. The present invention also relates to an apparatus for loading and releasing a probe. The apparatus comprises a push pin and a groove to load and transfer the probe to a plurality of locations, such as different wells in the above-mentioned cartridge, to conduct the immunoassay test. | 2013-11-07 |
20130295592 | DETERMINATION OF TOTAL ANALYTE CONCENTRATION - Methods and reagents are disclosed for determining a total amount of an analyte in an unknown sample suspected of containing the analyte in the presence of endogeneous interfering substances. The methods involve measuring an amount [Y] of the portion of the analyte in the unknown sample that is bound by endogeneous binding substances employing an assay for the analyte. An amount [Z] of analyte in the unknown sample that is not bound by endogeneous binding substances is determined by the formula: [Z]=a[Y]+b, wherein “a” and “b” are predetermined by conducting the assay on samples containing known amounts of the analyte but substantially free from endogeneous interfering substances. Adding [Y] and [Z] yields the total amount [X] of the analyte in the unknown sample. | 2013-11-07 |
20130295593 | TRUNCATED HUMAN VITAMIN D BINDING PROTEIN AND MUTATION AND FUSION THEREOF AND RELATED MATERIALS AND METHODS OF USE - Vitamin D binding proteins (DBP), in particular truncated DBP and mutated, truncated DBP, as well as fusion proteins thereof, nucleic acid molecules encoding same, vectors, host cells, and methods, kits and solid supports for determining the total amount of 25-hydroxy vitamin D | 2013-11-07 |
20130295594 | METHODS AND COMPOSITIONS FOR THE DIAGNOSIS OF CROHN'S DISEASE - The present invention provides methods and materials, including kits, to evaluate Crohn's disease, including to diagnose, monitor, or determine the efficacy of treatment for Crohn's Disease. The methods involve determining the presence, absence, or level of zonulin in a subject sample. In certain embodiments, the need for more laborious and/or invasive tests to monitor disease state is minimized or obviated. | 2013-11-07 |
20130295595 | MODIFIED PROKARYOTIC UBIQUITIN-LIKE PROTEIN AND METHODS OF USE THEREOF - Methods for making and using substrates of deamidase of prokaryotic ubiquitin-like protein (Dop) are described herein. More particularly, modified prokaryotic ubiquitin-like protein (Pup) and functional fragments thereof that serve as exemplary Dop substrates are described and encompassed herein. Screening methods to identify modulators of Dop and Pup activity and use of modulators identified thereby are also described. Methods of using modulators that are identified as inhibitors of Dop and Pup activity for treating diseases/conditions associated with | 2013-11-07 |
20130295596 | RAPID METHOD FOR TARGETED CELL (LINE) SELECTION - The present invention relates to a process for the prediction of cell culture performance data of sample cells, a process for the isolation of said cells and a device for the prediction of cell culture performance data of sample cells. | 2013-11-07 |
20130295597 | AUTOMATED SYSTEM FOR SAMPLE PREPARATION AND ANALYSIS - A sample preparation and analysis system. The system | 2013-11-07 |
20130295598 | CIRCULATION SYSTEM - A self-contained circulation system, which supports the formation of capillaries in capillary growth sections and allows the formation of micro organoids and/or micro tissue sections for monitoring the effect of one or more test compounds and determining efficacy, side-effects, biosafety, metabolites, mode of action or organ regeneration as well as methods of establishing such micro organoids and/or micro tissue in the self-contained circulation system. | 2013-11-07 |
20130295599 | Method for Imaging Zinc Activation within a Mitochondrion Using a Two-Photon Fluorescent Probe, and Method for Manufacturing the Two-Photon Fluorescent Probe - Provided is a two-photon fluorescent probe, and more particularly, a two-photon fluorescent probe which is one or more selected from compounds represented by Formulae 1 and 2, a method for manufacturing the same, and an imaging method of zinc ions within the mitochondrion using the same. Since two probes are introduced into one molecule, the two-photon fluorescent probe of the present invention can selectively dye the mitochondria, simultaneously with reacting with zinc ions, thereby generating intense fluorescence. Thus, the two-photon fluorescent probe of the present invention can be used for the imaging of zinc ion distribution and activation within the mitochondrion in living cells or intact biological tissues. | 2013-11-07 |
20130295600 | Process analysis system with sterile sampling of mechanically-sensitive material from a bioreactor - Method for transport of suspensions containing mechanically sensitive material with a sample-transporting device comprising transport conduits and at least one system for accelerating a sample or aliquot through the transport conduit from at least two burets. | 2013-11-07 |
20130295601 | Systems and Methods for Testing Drugs and Drug Delivery Systems - A system is provided that simulates the in vivo micro-environment of three-dimensional cellular structures or bodies, such as tumors. The system simulates the pressure gradients and fluid flows of the vascular and lymphatic systems as well as the interstitial and capillary transport mechanisms between the 3D cellular structure and the vascular and lymphatic systems. The system can be used to introduce drugs or drug delivery carriers to a tumor, for example, to assess the uptake capability and effect on the tumor. The system maintains the viability of the tumor cells for a sufficiently long period of time to permit testing of several different drugs and/or delivery carriers. | 2013-11-07 |
20130295602 | METHODS, SYSTEMS AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS - Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases. | 2013-11-07 |
20130295603 | De-mannosylation of phosphorylated N-glycans - Methods for demannosylating phosphorylated N-glycans on a glycoprotein are described that use a mannosidase capable of hydrolyzing a terminal alpha-1,2 mannose linkage when the underlying mannose is phosphorylated. | 2013-11-07 |
20130295604 | METHODS FOR PRODUCING MODIFIED GLYCOPROTEINS - Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man | 2013-11-07 |
20130295605 | METHOD FOR PRODUCING A POLYPEPTIDE IN YARROWIA LIPOLYTICA - This invention relates to a method for the production of proteins, preferably, heterologous proteins, under the regulation of the hp4d promoter, in | 2013-11-07 |
20130295606 | SYNTHETIC PATHWAY ENZYMES FOR THE PRODUCTION OF ARGYRINS - The invention provides the amino acid sequences comprised in or constituting the synthetic pathway enzymes participating in the production of Argyrins, as well as the nucleic acid sequences encoding the synthetic pathway enzymes participating in the production of Argyrins, as well as genetically manipulated microorganisms containing nucleic acid sequences encoding the synthetic pathway enzymes for the production of Argyrins, e.g. for inserting one or more of these coding sequences, mutating in a targeted manner one or more of these nucleic acid sequences, in a wild type producer micro-organism or in a heterologous micro-organism, for the production of Argyrins. | 2013-11-07 |
20130295607 | PRODUCT AND PROCESS FOR TRANSFORMATION OF THRAUSTOCHYTRIALES MICROORGANISMS - Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms. | 2013-11-07 |
20130295608 | YEAST STRAIN FOR THE PRODUCTION OF PROTEINS WITH MODIFIED O-GLYCOSYLATION - Lower eukaryotic host cells have been recombinantly engineered to produce glycoprotein having human-like O-glycosylation. The glycoproteins are useful for the production of glycoprotein compositions with advantages for the production of human therapeutics. | 2013-11-07 |
20130295609 | PLANT EXPRESSION SYSTEM - A plant expression system and methods for expressing a protein of interest in a plant are provided. The plant expression system comprises a first nucleic acid sequence regulatory region sequence, operatively linked with a one or more than one comovirus enhancer, a nucleotide sequence of interest, one or more than one geminivirus amplification elements, and a second nucleic acid encoding a geminivirus replicase. The method of producing a protein of interest in a plant, involves introducing the plant expression system into a plant, or portion of the plant, and incubating the plant or the portion of the plant under conditions that permit the expression of the nucleotide sequence and producing the protein of interest. | 2013-11-07 |
20130295610 | CHIMERIC ANTIGENS FOR ELICITING AN IMMUNE RESPONSE - Disclosed herein are compositions and methods for eliciting immune responses against antigens. In particular embodiments, the compounds and methods elicit immune responses against antigens that are otherwise recognized by the host as “self” antigens. The immune response is enhanced by presenting the host immune system with a chimeric antigen comprising an immune response domain and a target binding domain, wherein the target binding domain comprises a xenotypic antibody fragment. By virtue of the target binding domain, antigen presenting cells take up, process, and present the chimeric antigen, eliciting both a humoral and cellular immune response. | 2013-11-07 |
20130295611 | HUMANIZED ANTIBODY MOLECULES SPECIFIC FOR IL-31 - The invention provides humanized mouse anti-human IL-31 antibodies and antibody fragments that are capable of binding IL-31 and thereby neutralizing, inhibiting, limiting, or reducing the proinflammatory or pro-pruritic effects of IL-31. | 2013-11-07 |
20130295612 | ANTI-GLYPICAN-3 ANTIBODY HAVING IMPROVED KINETICS IN PLASMA - A method of modulating the plasma half-life of anti-glypican 3 antibody, a pharmaceutical composition comprising as an active ingredient the anti-glypican 3 antibody that has a plasma half-life that has been modulated, a method of preparing the anti-glypican 3 antibody and a pharmaceutical composition comprising the anti-glypican 3 antibody as an active ingredient are provided. Disclosed is a method of modulating the plasma half-life of anti-glypican 3 antibody by modifying an amino acid residue that is exposed on the surface of the anti-glypican 3 antibody; and anti-glypican 3 antibody that has a plasma half-life that has been modulated by amino acid residue modification, a pharmaceutical composition comprising as an active ingredient the anti-glypican 3 antibody, and a method of preparing the anti-glypican 3 antibody and producing a pharmaceutical composition comprising the anti-glypican 3 antibody as an active ingredient. | 2013-11-07 |
20130295613 | ANIMAL CELL CULTURING METHOD - While a desired protein is prepared by culturing an animal cell that produces the protein to cause the protein to be produced, level of heterogeneity components of the protein is modulated by performing the culture at a normal culture temperature for a certain period and then continuing the culture at a culture temperature lowered to 25-35° C. | 2013-11-07 |
20130295614 | NUCLEOTIDE SEQUENCES, METHODS, KIT AND A RECOMBINANT CELL THEREOF - The present disclosure relates to recombinant adeno-associated virus (AAV) vector serotype, wherein the capsid protein of AAV serotypes is mutated at single or multiple sites. The disclosure further relates to an improved transduction efficiency of these mutant AAV serotypes. The AAV serotypes disclosed are AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10. The instant disclosure relates to nucleotide sequences, recombinant vector, methods and kit thereof. | 2013-11-07 |
20130295615 | METHOD FOR EXPRESSION OF SMALL ANTIVIRAL RNA MOLECULES WITH REDUCED CYTOTOXICITY WITHIN A CELL - In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. | 2013-11-07 |
20130295616 | RECOMBINANT YEAST AND SUBSTANCE PRODUCTION METHOD USING THE SAME - Substance productivity is improved by introducing a metabolic pathway for synthesis of acetyl-CoA or acetic acid from glucose-6-phosphate into yeast. Acetic acid productivity, acetyl-CoA productivity, and productivity of a substance made from acetyl-CoA-derived are improved by attenuating genes involved in the glycolytic system of yeast and introducing a phosphoketolase gene into the yeast. | 2013-11-07 |
20130295617 | METHODS AND COMPOSITIONS FOR PRODUCTION OF TREHALULOSE - The present invention provides a method of producing trehalulose, the method comprising: (a) contacting a sucrose solution with a sucrose isomerase enzyme to produce a sucrose-enzyme mixture; and (b) incubating the sucrose-enzyme mixture of (a) at a temperature of less than 10° C. for a period of time sufficient to convert the sucrose in the sucrose-enzyme mixture to a product comprising trehalulose. | 2013-11-07 |
20130295618 | PROCESS FOR PRODUCING A PARTICULATE COMPOSITION COMPRISING ANHYDROUS CRYSTALLINE 2-O-ALPHA-D-GLUCOSYL-L-ASCORBIC ACID - The invention provides a process for enabling the production of a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside that does not significantly cake even when the production yield of ascorbic acid 2-glucoside does not reach 35% by weight. The process for producing a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside, which comprises allowing a CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid and then allowing a glucoamylase to act on the resulting solution to obtain a solution with an ascorbic acid 2-glucoside production yield of at least 27%, purifying the obtained solution to increase the ascorbic acid 2-glucoside content to a level of over 86% by weight, precipitating anhydrous crystalline ascorbic acid 2-glucoside by a controlled cooling method or pseudo-controlled cooling method, collecting the precipitated anhydrous crystalline ascorbic acid 2-glucoside, and ageing and drying the collected anhydrous crystalline ascorbic acid 2-glucoside. | 2013-11-07 |
20130295619 | ACIDOTHERMUS CELLULOYTICUS XYLANASE - A thermophilic endo-beta-1,4-xylanase derived from | 2013-11-07 |
20130295620 | SACCHAROMYCES CEREVISIAE STRAINS - A method for producing a strain of | 2013-11-07 |
20130295621 | Method for Producing a Target Substance by Fermentation - A target substance can be produced by culturing a bacterium having an ability to produce 2-ketoglutaric acid or a derivative thereof, and an ability to produce xylonic acid from xylose, which is imparted with xylonate dehydratase activity, 2-keto-3-deoxyxylonate dehydratase activity and 2-ketoglutaric semialdehyde dehydrogenase activity, or in which these activities are enhanced, in a medium containing xylose as a carbon source to produce and accumulate the target substance in the medium, and collecting the target substance from the medium. | 2013-11-07 |
20130295622 | PROCESS FOR THE PREPARATION OF NEBIVOLOL - The present invention relates to a novel process for the synthesis of the Nebivolol product depicted in Scheme 1, comprised of a reduced number of high-yield steps, and characterized by the enzymatic resolution of the chroman ester precursor. | 2013-11-07 |
20130295623 | BICARBONATE TRIGGER FOR INDUCING LIPID ACCUMULATION IN ALGAL SYSTEMS - The present invention provides bicarbonate containing and/or bicarbonate-producing compositions and methods to induce lipid accumulation in an algae growth system, wherein the algae growth system is under light-dark cycling condition. By adding said compositions at a specific growth stage, said methods lead to much higher lipid accumulation and/or significantly reduced total time required for accumulating lipid in the algae growth system. | 2013-11-07 |
20130295624 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. | 2013-11-07 |
20130295625 | Apparatus and Method for Treating Organic Waste - The present invention relates to an apparatus for treating organic waste including: a first circulation line having a first circulation pump connected thereon so as to supply a portion of the organic waste being acid fermented in an acid fermenter to a methane fermenter; a second circulation line having a second circulation pump connected thereon so as to supply a portion of anaerobic digestive fluid methane fermented in the methane fermenter to the acid fermenter; and vortex generating means having a plurality of first, second and third nozzles disposed in the methane fermenter so as to allow the anaerobic digestive fluid in the methane fermenter to be agitated by vortices generated thereof. | 2013-11-07 |
20130295626 | PROTEIN COMPLEX HAVING ACTIVITY CATALYZING ASYMMETRIC OXIDATION REACTION AND PROCESS FOR PRODUCING THE SAME - A process for producing a cross-linked crystallized protein complex, which comprises: a first step of concentrating a crude protein derived from an animal or plant; a second step of encapsulating the protein in a gel, to thereby allow the protein to undergo air oxidation, and then extracting a protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation; and a fourth step of cross-linking the precipitated protein complex. Alternatively, by use of a fifth step of drying (FD) the obtained crosslinked crystallized protein complex, to thereby form a powder. As a result, there is provided an enzyme which is stable at room temperature storage, and has an activity in catalyzing an asymmetric oxidation reaction. That is, there is provided a useful material which enables an efficient enzyme-mimetic reaction under a mild condition. | 2013-11-07 |
20130295627 | PROCESS FOR PRODUCING METHYL BUTENOL (2-METHYL-3-BUTEN-2-OL) - Disclosed is a process for producing 2-methyl-3-buten-2-ol (MBO). The process is carried out by fermenting a hydrocarbon in a fermentation medium in the presence of a MBO-producing microorganism to produce the MBO. The MBO is removed from the fermentation medium to maintain a concentration of MBO non-toxic to the MBO-producing microorganism by flowing a gas through or across the fermentation medium to effectively remove at least a portion of the MBO into a vapor phase region of the fermentation medium. The rate of removal is measured according to the amount of MBO maintained in the vapor phase region, which is measured according to the vapor pressure of the MBO in the vapor phase region. | 2013-11-07 |
20130295628 | PROCESSES FOR PRODUCING ENERGY-DENSE BIOMASS AND SUGARS OR SUGAR DERIVATIVES, BY INTEGRATED HYDROLYSIS AND TORREFACTION - This invention provides processes to convert biomass into energy-dense biomass for combustion, alone or in combination with another solid fuel. In some variations, biomass is extracted to produce an extract liquor containing hemicellulosic oligomers and cellulose-rich solids; hemicellulosic oligomers are removed; and the cellulose-rich solids are torrefied to produce energy-dense biomass. In some embodiments, hydrotorrefaction is employed to produce hydrophobic, energy-dense biomass in an energy-efficient process that avoids intermediate drying between extraction/hydrolysis and torrefaction. The energy-dense biomass may be pelletized or directly combusted or gasified. The hemicellulosic oligomers may be hydrolyzed to fermentable sugars and then fermented to ethanol or other products, or further reacted to produce furfural or other products. | 2013-11-07 |
20130295629 | CONTINUOUS OR SEMI-CONTINUOUS PROCESS FOR TREATING BIOMASS TO PRODUCE MATERIALS USEFUL FOR BIOFUELS - Fermentable sugar useful for the production of biofuels is produced from biomass in a continuous or semi-continuous manner by providing pumpable biomass. | 2013-11-07 |
20130295630 | METHOD OF PRODUCING ETHANOL - A method of producing ethanol wherein feedstock is broken down and mixed with yeast in a fermenting tank at an optimum predetermined temperature. An alcohol additive is added to the mixture in the fermentation tank. Once fermented, the mixture is distilled and stored. | 2013-11-07 |
20130295631 | COMBINATORIAL DESIGN OF HIGHLY EFFICIENT HETEROLOGOUS PATHWAYS - The present disclosure relates to the production of highly efficient heterologous pathways in host cells by identifying favorable enzyme and/or promoter combinations. In particular the present disclosure provides methods for assembly and selection of multi-step xylose and arabinose/xylose utilization pathways from a library of fungal enzymes. The present disclosure further provides compositions containing favorable enzyme combinations, as well as recombinant yeast expressing such combinations, and methods of use for bioconversion of pentose sugars. Also provided are compositions and methods involving favorable expression patterns identified by utilization of combinations of promoters of varying strengths. Provided herein are methods for assembly and selection of multi-step xylose, arabinose/xylose, and cellobiose utilization pathways from a library of promoters of varying strengths. The present disclosure further provides compositions containing heterologous enzyme-coding polynucleotides under the control of favorable promoters, as well as recombinant yeast expressing such enzymes, and methods of their use for bioconversion of pentose and/or hexose sugars. | 2013-11-07 |
20130295632 | ISOPRENE SYNTHASE VARIANTS WITH IMPROVED SOLUBILITY FOR PRODUCTION OF ISOPRENE - The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells. | 2013-11-07 |
20130295633 | COMPOSITIONS AND METHODS OF PGL FOR THE INCREASED PRODUCTION OF ISOPRENE - Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity. | 2013-11-07 |
20130295634 | PRODUCTION OF BIOCHAR ABSORBENT FROM ANAEROBIC DIGESTATE - A novel carbon absorption material is described which is formed from anaerobic digestate. The material has a hollow tubular structure and is particularly advantageous in converting hydrogen sulfide in biogas and in absorbing the converted sulfur and sulfur compounds from biogas into its structure. The material after use as a hydrogen sulfide absorbent has value as a horticultural or agricultural product or as a sulfur impregnated activated carbon. The process for producing this novel carbon absorption material is described. In an embodiment, the process described uses in particular, a humidified inert gas over a temperature range of between about 500° C. to 900° C. to convert anaerobic digestate to an active carbon absorbent. The thermal treatment is relatively mild and retains the fibrous structure of the source material while removing cellulosic and hemicellulosic components from the anaerobic digestate. | 2013-11-07 |
20130295635 | System for Optical Stimulation of Target Cells - Various systems and methods are implemented for controlling stimulus of a cell. One such method is implemented for optical stimulation of a cell expressing a NpHR ion pump. The method includes the step of providing a sequence of stimuli to the cell. Each stimulus increases the probability of depolarization events occurring in the cell. Light is provided to the cell to activate the expressed NpHR ion pump, thereby decreasing the probability of depolarization events occurring in the cell. | 2013-11-07 |
20130295636 | Isolation and Functional Identification of Cells - The invention relates to a method for tagging, identifying and isolating a target cell, wherein the cell is contacted with a capture compound comprising a small molecule moiety, for example a pharmaceutical drug molecule, capable of selectively binding with the cell surface of the target cell, and a function that is capable to covalently or quasi-covalently bind to a surface as a marker or tagging function. In some embodiments the capture compound also includes a photoactivatable reactivity function. The capture compound attaches to a particle that mediates identification or isolation of the tagged cell. | 2013-11-07 |
20130295637 | FIBROUS SUBSTRATES FOR CELL PROPAGATION AND DIFFERENTIATION - The present invention relates to a method of releasably encapsulating pluripotent embryonic stem cells in a degradable continuous polyionic fiber for tissue culture, wherein the encapsulated embryonic stem cells are able to maintain a pluripotent phenotype in tissue culture; the method comprising (a) contacting an aqueous solution of a polyanion with an aqueous solution of a polycation to form an interface between the aqueous solution of polyanion and the aqueous solution of polycation, and wherein the aqueous solution of polyanion or the aqueous solution of polycation or both the aqueous solution of polyanion and the aqueous solution of polycation comprises a suspension of pluripotent embryonic stem cells; (b) drawing a continuous polyionic fiber which comprises encapsulated pluripotent embryonic stem cells from the interface; (c) passing the continuous polyionic fiber comprising encapsulated pluripotent embryonic stem cells in a continuous process through a solution which reduces secondary complexation of the components of the polyionic fiber. | 2013-11-07 |
20130295638 | SYNTHETIC BRASSICA-DERIVED CHLOROPLAST TRANSIT PEPTIDES - This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom. | 2013-11-07 |
20130295639 | Branched Polymers - The present invention is directed to branched reactive water-soluble polymers comprising at least two polymer arms, such as poly(ethylene glycol), attached to a central aliphatic hydrocarbon core molecule through heteroatom linkages. The branched polymers bear at least one functional group for reacting with a biologically active agent to form a biologically active conjugate. The functional group of the branched polymer can be directly attached to the aliphatic hydrocarbon core or via an intervening linkage, such as a heteroatom, -alkylene-, —O-alkylene-O—, -alkylene-O-alkylene-, -aryl-O—, —O-aryl-, (—O-alkylene-) | 2013-11-07 |
20130295640 | Methods and Reagents for Preparing Multifunctional Probes - Multifunctional probes are synthesized in a single step using peptide scaffold-based multifunctional single-attachment-point reagents. To obtain multifunctional probes of the invention, a substrate (e.g., a nanoparticle, polymer, antibody, protein, low molecular weight compound, drug, etc.) is reacted with a multifunctional single-attachment-point (MSAP) reagent. The MSAP reagents can include three components: (i) a peptide scaffold, (ii) a single chemically reactive group on the peptide scaffold for reaction of the MSAP with a substrate having a complementary reactive group, and (iii) multiple functional groups on the peptide scaffold. The peptide scaffold can include any number of residues; however, for ease of synthesis and reproducibility in clinical trials, it is preferred to limit the residues in the peptide to 20 or less. The reagent can be prepared to yield a predetermined stoichiometric ratio of the functional groups on the scaffold such that the probe has a fixed stoichiometric ratio of the functional groups. | 2013-11-07 |
20130295641 | Production of Conjugates - A method of reacting a first chemical entity and a second chemical entity to form a conjugate in which the first and second chemical entities are covalently bound with respect to each other, comprises bringing into simultaneous contact the first chemical entity, the second chemical entity and a thiol generator, wherein the thiol generator reacts with the first chemical entity in a thiolation reaction resulting in formation of a free sulfhydryl group on the first chemical entity, and the free sulfhydryl group reacts with the second chemical entity to form the conjugate, and wherein the second chemical entity is polyvalent with respect to its reactivity with sulfhydryl groups. The present invention primarily differs from the prior art in that no separation step is involved between reaction of the thiol generator and first chemical entity and reaction with the second chemical entity. The invention also provides a conjugation kit. | 2013-11-07 |
20130295642 | PROTEIN COMPLEX HAVING ACTIVITY CATALYZING ASYMMETRIC OXIDATION REACTION AND PROCESS FOR PRODUCING THE SAME - A process for producing a cross-linked crystallized protein complex, which comprises: a first step of concentrating a crude protein derived from an animal or plant; a second step of encapsulating the protein in a gel, to thereby allow the protein to undergo air oxidation, and then extracting a protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation; and a fourth step of cross-linking the precipitated protein complex. Alternatively, by use of a fifth step of drying (FD) the obtained crosslinked crystallized protein complex, to thereby form a powder. As a result, there is provided an enzyme which is stable at room temperature storage, and has an activity in catalyzing an asymmetric oxidation reaction. That is, there is provided a useful material which enables an efficient enzyme-mimetic reaction under a mild condition. | 2013-11-07 |
20130295643 | NON-CYTOTOXIC PROTEIN CONJUGATES - The present invention is directed to non-cytotoxic protein conjugates for inhibition or reduction of exocytic fusion in a nociceptive sensory afferent cell. The protein conjugates comprise: (i) a dynorphin Targeting Moiety (TM), wherein the TM is an agonist of a receptor present on a nociceptive sensory afferent cell, and wherein the receptor undergoes endocytosis to be incorporated into an endosome within the nociceptive sensory afferent cell; (ii) a non-cytotoxic protease or a fragment thereof, wherein the protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of the nociceptive sensory afferent cell; and (iii) a Translocation Domain, wherein the Translocation Domain translocates the protease or protease fragment from within the endosome, across the endosomal membrane, and into the cytosol of the nociceptive sensory afferent cell. Nucleic acid sequences encoding the protein conjugates, methods of preparing same and uses thereof are also described. | 2013-11-07 |
20130295644 | Polypeptides Having Succinyl-CoA: Acetoacetate Transferase Activity and Polynucleotides Encoding Same - The present invention relates to isolated protein complexes having succinyl-CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes. | 2013-11-07 |
20130295645 | Method for in vitro Recombination - The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. | 2013-11-07 |
20130295646 | METHOD FOR EXTRACTING A PROTEIN FROM MILK - The invention relates to a method for extracting a protein from milk, having at least one hydrophobic pocket and a negative charge to the natural pH of milk, that comprises the following steps: a) skimming and delipidation of the milk; b) passing the delipidated and skimmed fraction containing the protein on a chromatographic substrate on which is grafted a ligand having both a hydrophobic characteristic and an ionic characteristic in pH conditions enabling the protein to be retained on the substrate, the pH being higher than 4.6; c) elution of the protein; d) purification of the eluted fraction by removing the milk proteins from the eluted fraction; and e) recovering the protein. | 2013-11-07 |
20130295647 | METHOD OF PRODUCING BACTERIOPHAGE PREPARATIONS COMPRISING PURIFICATION USING AFFINITY CHROMATOGRAPHY - The proposed method facilitates the single-stage and at the same time effective purification of phage preparations for therapeutic uses, and facilitates the maintenance of bacteriophage antibacterial activity both in the case of displacement of the bacteriophage from the resin and its proteolytic release. The protein modification of the phage capsid with appropriate binding motifs makes it possible to purify therapeutically bacteriophage strains using affinity chromatography. The proposed method is useful in the display of selected polypeptided on a bacteriophage capsid without the need to genetically modify the bacteriophage, and thus makes it possible to produce phage preparations for various uses using wild-type phages occurring naturally or others not additionally modified for phage-display purposes. | 2013-11-07 |
20130295648 | PHARMACEUTICAL COMPOSITION FOR TREATING CANCER - The present invention relates to increase in survival of mammals suffering from desmocollin 3 expressing cancers. | 2013-11-07 |
20130295649 | Photobioreactor System with High Specific Growth Rate and Low Dilution Rate - Systems and methods for growing photosynthetic cells that may be used to produce a biomass. The systems and methods recycle liquid and can produce a high cell concentration harvested biomass. | 2013-11-07 |
20130295650 | BIOREMEDIATION OF PERSISTENT ORGANIC POLLUTANTS USING THERMOPHILIC BACTERIA - The present application relates to a method of degrading organic contaminants in contaminated soil, sediment or wastewater, the method being carried out by treating the contaminated soil, sediment or wastewater with thermophilic bacterium capable of degrading the organic contaminants. | 2013-11-07 |
20130295651 | Method for Treating Textile with Endoglucanase - The present invention relates to the method for manufacturing textile, by treating textile with an isolated polypeptide having endoglucanase activity, especially in biostoning and bio-polishing process. | 2013-11-07 |
20130295652 | SYSTEM AND METHOD FOR ISOLATION OF SAMPLES - Systems and methods for isolating samples are provided. The system comprises a first membrane and a second membrane disposed within an enclosure. First and second reservoirs can also be disposed within the enclosure and adapted to contain one or more reagents therein. A first valve can be disposed within the enclosure and in fluid communication with the first reservoir, the second reservoir, or both. The first valve can also be in fluid communication with the first or second membranes or both. The first valve can be adapted to selectively regulate the flow of the reagents from the first reservoir, through at least one of the first and second membranes, and into the second reservoir. | 2013-11-07 |
20130295653 | INTEGRATED ACTIVE FLUX MICROFLUIDIC DEVICES AND METHODS - The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. | 2013-11-07 |
20130295654 | Cooling in a Thermal Cycler Using Heat Pipes - A device for amplifying a nucleic acid sample may include a sample holder configured to receive a nucleic acid sample, a heating system configured to raise the temperature of the sample, a cooling system configured to lower the temperature of the sample, and a controller configured to operably control the heating system and the cooling system to cycle the device through a desired time-temperature profile. The cooling system may include at least one heat pipe and a heat sink and the at least one heat pipe may include a first portion disposed proximate to the sample holder and a second portion disposed proximate to the heat sink. | 2013-11-07 |
20130295655 | MICRO-FLUIDIC DEVICE - Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber. | 2013-11-07 |
20130295656 | Anaplasma Translocated Substrate-1 (Ats-1) and Sero-detection of Anaplasma phagocytophilum - Disclosed is the use of isolated Ats-1 protein in | 2013-11-07 |
20130295657 | Device and Method for the Detection of Particles - The present invention relates to devices and methods for the qualitative and/or quantitative detection of particles. In particular, the invention relates to devices for the detection of particles, comprising a reaction chamber formed within a chamber body between a first surface and a second surface, wherein the second surface is located opposite to the first surface, and one or more displacers, wherein the distance between the first surface and the second surface is variable via the one or more displacers at least in one or more parts of the surface area of the first surface and/or second surface. The invention also relates to corresponding methods for the detection of particles. | 2013-11-07 |
20130295658 | SYSTEMS AND METHODS FOR DIGESTION OF SOLID WASTE - This invention relates generally to systems and methods for digestion of solid waste that simplify solids handling. In certain embodiments, anaerobic methane extraction takes place for a period of time (e.g., from 1 to 4 weeks), after which an aerobic composting process begins in the same chamber. The organic waste remains in place and oxygen (e.g., in air) is forced into the chamber for an additional period of time (e.g., from 2 to 4 weeks). At the conclusion of the aerobic phase, the process yields a rough compost product that is stable and pathogen free. The rough compost can be further processed and blended to create high value engineered soils. | 2013-11-07 |
20130295659 | CLOSED TYPE PHOTO-BIO REACTING APPARATUS FOR MICROALGAE - Disclosed is a closed type photo-bio reacting apparatus for microalgae. The apparatus includes a reactor body, a hollow fiber membrane contact unit, a fluid circulating pump, a light source, and an angle adjusting lift. The reactor body cultures the microalgae. The hollow fiber membrane contact unit is disposed in the reactor body and supplies carbon dioxide to culture solution circulating in the reactor body. The fluid circulating pump circulates the culture solution in the reactor body. The light source irradiates light into the reactor body. The angle adjusting lift adjusts an inclination angle of the reactor body according to an irradiation angle of the light source. | 2013-11-07 |
20130295660 | BIOREACTOR SYSTEM - A bioreactor or culture vessel for incubation of one or more cell cultures, tissue biopsies, cell clusters, tissue-like structures, “prototissues” or similar samples. The bioreactor comprises an incubation chamber, a liquid reservoir and conduction means separated from each other by semipermeable membranes. Specifically, the invention provides a humidity chamber design which simplifies the construction of the bioreactor. | 2013-11-07 |
20130295661 | Methods and Systems for Removing Undissolved Solids Prior to Extractive Fermentation in the Production of Butanol - A method and system for efficiently producing a fermentative product alcohol such as butanol utilizing in situ product extraction are provided. The efficiency is obtained through separating undissolved solids after liquefying a given feedstock to create a feedstock and prior to fermentation, for example, through centrifugation. Removal of the undissolved solids avoids problems associated with having the undissolved solids present during in situ production extraction, and thereby increases the efficiency of the alcohol production. | 2013-11-07 |
20130295662 | Apparatus and Method for Growing Cells - A system and method for culturing cells is provided that includes an extra-capillary space between at least one permeable hollow fiber and an enclosed chamber. Cells are placed in the extra-capillary space to grow. One or more reservoirs containing cell-culture media and/or an oxygen-containing gas are provided in communication with the at least one fiber. The system is configured to generate alternating flows of both the cell-culture media and the gas through a lumen of the hollow fiber(s), thereby passing both nutrients and gas through the walls of the fiber(s) to the cells in the extra-capillary space to provide a suitable environment for growth and/or proliferation of the cells. Flows of liquid and gas through the hollow fiber(s) can be produced by gravity and/or various pumping configurations. | 2013-11-07 |
20130295663 | Pressurizable Cartridge for Polymerase Chain Reactions - Methods and apparatus for use in connection with the performance of the polymerase chain reaction are provided. An exemplary sample processing module is described that includes a sample assembly and a PCR assembly, the sample processing module being configured to hold the sample therein at a pressure higher than ambient pressure. A sample is added to the sample assembly at the time of use, which is then connected to the PCR assembly. Embodiments of the cartridge include a flow restriction device that enables or aids in creating a higher pressure within the reaction vial. The sample is introduced into a PCR reaction vial, which contains all of the constituents of a PCR reaction mixture that are necessary to process the sample and provide amplified DNA of interest, if that DNA was present in the sample. | 2013-11-07 |
20130295664 | MICROBIAL AIR SAMPLER - An air sampler device has a top plate and a bottom plate, and receives a Petri dish between the top plate and the bottom plate. The top plate includes 283 substantially small holes. The bottom plate has a deepened center well formed in the top surface. Elongated slots are formed in the top surface which extend out from the well. The slots have distal ends which extend beyond the Petri dish. Air is drawn into the sampler by a vacuum tube through an air port which communicates with the center well. Air is pulled into the 283 holes in the top plate and strikes the capture material in the Petri dish. The air then travels up over the sides of the dish, into the distal ends, through the slots, and into the center well, where it exits out of the vacuum air port. | 2013-11-07 |
20130295665 | VCP-Based Vectors for Algal Cell Transformation - Provided herein are exemplary vectors for transforming algal cells. In exemplary embodiments, the vector comprises a Violaxanthin-chlorophyll a binding protein (Vcp) promoter driving expression of an antibiotic resistance gene in an algal cell. Embodiments of the invention may be used to introduce a gene (or genes) into the alga | 2013-11-07 |
20130295666 | CRYSTALS OF GLUCOKINASE REGULATORY PROTEIN (GKRP) - The present invention pertains to crystals of glucokinase regulatory protein (GKRP) and of GKRP variants, to the molecular biology of certain GKRP variants, to processes for the crystallization of GKRP and GKRP variants, to such crystals and corresponding structural information obtained by X-ray crystallography. Such crystals and crystallographic data can be used for the identification of compounds that bind to GKRP, especially of compounds that inhibit GKRP or interfere with the interaction of GKRP with its natural interacting partner Glucokinase (GK). | 2013-11-07 |
20130295667 | SERUM-FREE MAMMALIAN CELL CULTURE MEDIUM, AND USES THEREOF - The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells. | 2013-11-07 |
20130295668 | Synergistic Selenopeptide Formulations For The Protection of Dermal Papilla Cells - The present invention discloses selenium peptide based synergistic compositions for the protection (morphology and viable numbers) of dermal papilla cells. The synergistic compositions disclosed in the present invention comprise (a) 1-O-galloyl-β-D-glucose (β-glucogallin) or 1-O-galloyl-β-D-glucose (β-glucogallin) and gallates (b) concentrate of liquid endosperm of | 2013-11-07 |
20130295669 | METHOD FOR CONTROLLING BINDING OF CELLS TO A SUBSTRATE - The invention relates to a method for promoting the adhesion of cells to a substrate to which these cells usually have no or only low affinity, wherein the adhesion of the cells to the substrate is promoted by supplying the cells with the non-muscle myosin II inhibitor Blebbistatin so as to enable the cells to attach to surfaces to which they otherwise would not have sufficient affinity. Surprisingly, supplying the cells with the inhibitor enhances the capability of these cells to attach to surfaces to which they usually have no or only low affinity, for example, PTFE (Teflon®). The invention further concerns uses of the non-muscle myosin II inhibitor Blebbistatin and devices having at least one surface which is coated with cells that have no or only low affinity to said surface. | 2013-11-07 |
20130295670 | Enrichment of Stem Cells from Adult Tissues - Subjecting a heterogeneous cell population (one with both stem cells and non-stem cells) to extreme stress selectively eliminated the non-stem cells and resulted in the enrichment of stem cells in the population. The stress can take many forms, including without limitation, cell toxins, high temperature, high salt, and low oxygen (hypoxic) conditions. The number of stem cells remaining after stress were increased, and showed increased expression of traditional stem cell markers. The stem cells were shown to be capable of proliferation and differentiation into multiple types of cells. This method allows purification of stem cells from adult heterogeneous cell populations on a large scale basis without requirement of expensive equipment, and without requiring the presence of cell surface markers. Stem cells produced by the above method can be used for clinical applications, including tissue engineering. | 2013-11-07 |
20130295671 | METHOD FOR PRODUCING NK CELL-ENRICHED BLOOD PREPARATION - It is intended to provide a method for producing an NK cell-enriched blood preparation, which is low invasive and is capable of conveniently and rapidly growing NK cells, etc. in blood collected from an organism. The NK cells in blood are stimulated with NK cell growth-stimulating factors comprising an anti-CD16 antibody, OK432, an anti-CD3 antibody, and a cytokine. Then, the blood is cultured at a physiological cell temperature to produce an NK cell-enriched blood preparation. | 2013-11-07 |
20130295672 | STEM CELL CULTURE MEDIA AND METHODS - The present invention provides novel stem culture compositions and methods. | 2013-11-07 |
20130295673 | SYSTEMS AND METHODS FOR PROCESSING CELLS - The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user. | 2013-11-07 |
20130295674 | METHOD OF EXTRACTING EMBRYOS FROM KERNELS OF CORN - A method is provided for extracting embryos from the kernels of an ear of corn. In various embodiments, the method includes receiving an extraction solution in a vessel, receiving at least a portion of an ear of corn in the vessel, the portion of the ear of corn including a plurality of kernels having embryos and intact pericarps, sealing the vessel, subjecting the sealed vessel to a vigorous shaking motion to create an embryo mixture, and collecting the embryo mixture. In some embodiments, embryos are separated from the embryo mixture. | 2013-11-07 |