40th week of 2015 patent applcation highlights part 33 |
Patent application number | Title | Published |
20150275186 | PRODUCTION OF VIRAL VACCINES IN SUSPENSION ON AVIAN EMBRYONIC DERIVED STEM CELL LINES - The present invention relates to the development and manufacturing of viral vaccines. In particular, the invention relates to the field of industrial production of viral vectors and vaccines, more in particular to the use of avian embryonic stem cells, preferably the EBx® cell line derived from chicken embryonic stem cells, for the production of viral vectors and viruses. The invention is particularly useful for the industrial production of viral vaccines to prevent viral infection of humans and animals. | 2015-10-01 |
20150275187 | FURFURAL-RESISTANT GENE AND FURFURAL-RESISTANT STRAINS COMPRISING THE SAME - The furfural-resistant strain containing the furfural-resistant gene according to the present disclosure may be effectively grown in a furfural-containing medium. Accordingly, the problem that microorganism fermentation was difficult because toxic by-products such as furfural are contained in a hydrolysate derived from inedible lignocellulosic biomass may be solved. Further, according to the method for producing a strain of the present disclosure, the resistant gene may be selected from relatively small number of target genes. Thus, time, cost and the like for developing the resistant strain may be saved. Further, this method for identifying genes may be broadly applied to methods for identifying various unknown functional genes in addition to the furfural-resistant gene. | 2015-10-01 |
20150275188 | ENHANCED PRODUCTION OF FATTY ACID DERIVATIVES - Genetically engineered cells and microorganisms are provided that produce fatty alcohols from the fatty acid biosynthetic pathway, as well as methods of their use. | 2015-10-01 |
20150275189 | POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from | 2015-10-01 |
20150275190 | CHIMERIC PROMOTERS AND METHODS OF USE - Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided. | 2015-10-01 |
20150275191 | TRITERPENE OXIDASE DERIVED FROM PLANT BELONGING TO GENUS GLYCYRRHIZA, GENE ENCODING THE SAME, AND METHOD OF USING THE SAME - Identification of a protein having an activity of oxidizing oleanane-type triterpene, and a gene encoding the protein, the protein and the gene, and use thereof are provided. For example, a protein having an activity of oxidizing oleanane-type triterpene obtained from a plant in the family Fabaceae, a gene encoding the protein and use thereof are provided. The protein is shown in, for example, SEQ ID NO: 4, 14 or 18, and the gene encoding the protein is shown in, for example, SEQ ID NO: 3, 13 or 17. A transformant into which the gene is introduced can be produced, and thereby a triterpene oxidase can be obtained. | 2015-10-01 |
20150275192 | POLYMERASE CAPABLE OF PRODUCING NON-DNA NUCLEOTIDE POLYMERS - The invention relates to a nucleic acid polymerase capable of producing a non-DNA nucleotide polymer from a DNA nucleotide polymer template, said polymerase comprising amino acid sequence having at least 36% identity to the amino acid sequence of SEQ ID NO: 1, wherein said amino acid sequence is mutated relative to the amino acid sequence of SEQ ID NO: 1, wherein said amino acid sequence comprises the mutations P657T, E658Q, K659H, Y663H, D669A, K671N, and T676I; wherein said amino acid sequence is further mutated relative to the amino acid sequence of SEQ ID NO: 1 at residue: E664 and wherein said amino acid sequence comprises the mutation E664K. The invention also relates to methods of making nucleotide polymers comprising use of this polymerase. Suitably the nucleotides are arabino nucleotides such as ARA: or FANA nucleotides. | 2015-10-01 |
20150275193 | METHODS AND PRODUCTS FOR EXPRESSING PROTEINS IN CELLS - The present invention relates in part to nucleic acids encoding proteins, therapeutics comprising nucleic acids encoding proteins, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods and products for altering the DNA sequence of a cell are described, as are methods and products for inducing cells to express proteins using synthetic RNA molecules. Therapeutics comprising nucleic acids encoding gene-editing proteins are also described. | 2015-10-01 |
20150275194 | Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains. | 2015-10-01 |
20150275195 | ELUTION OF BIOMOLECULES FROM MULTI-MODAL RESINS USING MES AND MOPS AS MOBILE PHASE MODIFIERS - The present invention relates to methods for purifying or enriching a biomolecule using multimodal resins and an elution buffer containing a Good's buffer. | 2015-10-01 |
20150275196 | MODIFIED SANTALENE SYNTHASE POLYPEPTIDES, ENCODING NUCLEIC ACID MOLECULES AND USES THEREOF - Provided are modified santalene synthase polypeptides, nucleic acid molecules encoding the modified santalene synthase polypeptides, and methods of using the modified santalene synthase polypeptides. The modified santalene synthase polypeptides include those that catalyze production of increased levels of terpenes or altered profiles thereof or both. | 2015-10-01 |
20150275197 | Small Enzyme-Containing Granules - The invention provides small enzyme-containing granules having an inorganic salt core and an enzyme-containing layer coated over the core, and methods for producing such granules. The majority of the enzyme granules are less than 300 μm in diameter. The granules are suitable for incorporation into compositions such as cleaning, textile processing, and animal feed compositions. | 2015-10-01 |
20150275198 | COMPOSITIONS, METHODS AND KITS FOR ISOLATING NUCLEIC ACIDS FROM BODY FLUIDS USING ANION EXCHANGE MEDIA - This invention provides compositions and methods for rapid separation, isolation and purification of nucleic acids from biological samples using anionic exchange media. The method can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution. The instant method is particularly advantageous as it permits the purification and identification of shorter fragments of nucleic acids from bodily fluids which, until now, had not been identified. | 2015-10-01 |
20150275199 | METHOD OF ISOLATING NUCLEIC ACID FROM SPECIMENS IN LIQUID-BASED CYTOLOGY PRESERVATIVES CONTAINING FORMALDEHYDE - Method, composition, kit and system for isolating amplifiable nucleic acid from specimens preserved in a liquid-based cytology preservative that contains formaldehyde. The technique relies on the use of 2-imidazolidone and a protease enzyme, such as proteinase K, at elevated temperatures. Advantageously, RNA can be isolated and used as a template in nucleic acid amplification reactions. | 2015-10-01 |
20150275200 | METHOD OF ISOLATING PURE MITOCHONDRIAL DNA - A method for preparing circular double stranded mitochondrial DNA (mtDNA) substantially free of genomic DNA (gDNA) comprising the steps of: providing a cellular lysate free of protein and RNA contaminants, precipitating cellular debris and proteins out of said lysate and obtaining a solution comprising purified circular double stranded mitochondrial DNA (mtDNA) and genomic DNA (gDNA), incubating said solution with an amount of Hind Exonuclease V for a time and at a temperature effective to cleave non-circular DNA and obtain circular double stranded mtDNA, incubating said circular double stranded mtDNA with an amount of Ampure beads effective to bind said circular double stranded mtDNA, washing said beads with ethanol, and eluting said mtDNA from said beads, wherein said method is free of ultra-centrifugation. | 2015-10-01 |
20150275201 | REPEAT PROTEIN FROM COLLECTION OF REPEAT PROTEINS COMPRISING REPEAT MODULES - A repeat protein from a collection of repeat proteins, wherein each repeat protein of said collection comprises a repeat domain, which comprises a set of consecutive repeat modules, wherein the repeat modules have the same fold and stack tightly to create a superhelical structure having a joint hydrophobic core, wherein each of the repeat modules is derived from one or more repeat units and wherein the repeat units comprise framework residues, which contribute to the folding topology of the repeat unit or contribute to an interaction with a neighboring repeat unit, and target interaction residues, which contribute to an interaction with a target substance, wherein the repeat proteins of the collection differ from other repeat proteins in the collection in at least one amino acid position of the repeat modules is described as are related pharmaceuticals and nucleic acid molecules. | 2015-10-01 |
20150275202 | CONTINUOUS DIRECTED EVOLUTION OF PROTEINS AND NUCLEIC ACIDS - The present invention discloses generalizable methods of evolving nucleic acids and proteins utilizing continuous directed evolution. The invention discloses methods of passing a nucleic acid from cell to cell in a desired function-dependent manner. The linkage of the desired function and passage of the nucleic acid from cell to cell allows for continuous selection and mutation of the nucleic acid. | 2015-10-01 |
20150275203 | RIBOREGULATOR COMPOSITIONS AND METHODS OF USE - The invention provides novel and versatile classes of riboregulators, including inter alia activating and repressing riboregulators, switches, and trigger and sink RNA, and methods of their use for detecting RNAs in a sample such as a well and in modulating protein synthesis and expression. | 2015-10-01 |
20150275204 | ANTISENSE ANTIVIRAL COMPOUNDS AND METHODS FOR TREATING A FILOVIRUS INFECTION - The present invention provides antisense antiviral compounds, compositions, and methods of their use and production, mainly for inhibiting the replication of viruses of the Filoviridae family, including Ebola and Marburg viruses. The compounds, compositions, and methods also relate to the treatment of viral infections in mammals including primates by Ebola and Marburg viruses. The antisense antiviral compounds include phosphorodiamidate morpholino oligonucleotides (PMOplus) having a nuclease resistant backbone, about 15-40 nucleotide bases, at least two but typically no more than half piperazine-containing intersubunit linkages, and a targeting sequence that is targeted against the AUG start site region of Ebola virus VP35, Ebola virus VP24, Marburg virus VP24, or Marburg virus NP, including combinations and mixtures thereof. | 2015-10-01 |
20150275205 | METHODS FOR MODULATING TAU EXPRESSION FOR REDUCING SEIZURE AND MODIFYING A NEURODEGENERATIVE SYNDROME - Disclosed herein are methods for reducing expression of Tau mRNA and protein in an animal with Tau antisense compounds. Also disclosed are methods for modulating splicing of Tau mRNA in an animal with Tau antisense compounds. Such methods are useful to treat, prevent, or ameliorate neurodegenerative diseases in an individual in need thereof. Examples of neurodegenerative diseases that can be treated, prevented, and ameliorated with the administration Tau antisense oligonucleotides include Alzheimer's Disease, Fronto-temporal Dementia (FTD), FTDP-17, Progressive Supranuclear Palsy, Chronic Traumatic Encephalopathy, Epilepsy, and Dravet's Syndrome. | 2015-10-01 |
20150275206 | POLYMER CONJUGATES FOR DELIVERY OF BIOLOGICALLY ACTIVE AGENTS - A delivery system for delivering oligonucleotides includes a conjugate includes a complexing agent including an oligonucleotide, a peptide nucleic acid or chimera thereof and a polymer covalently attached to the complexing agent. The complexing agent of the conjugate is adapted to complex a biologically active agent thereto after formation of the conjugate. | 2015-10-01 |
20150275207 | COMPOSITIONS AND METHODS FOR MODULATING POLYPEPTIDE LOCALIZATION IN PLANTS - Described herein are engineered multiple localization tags which, when translated and processed into peptides, will direct operably linked polypeptides to multiple subcellular locations. | 2015-10-01 |
20150275208 | SELECTIVE ANTISENSE COMPOUNDS AND USES THEREOF - The present invention provides oligomeric compounds. Certain such oligomeric compounds are useful for hybridizing to a complementary nucleic acid, including but not limited, to nucleic acids in a cell. In certain embodiments, hybridization results in modulation of the amount, activity, or expression of the target nucleic acid in a cell. In certain embodiments, hybridization results in selective modulation of the amount, activity, or expression of a target Huntingtin gene or Huntingtin transcript in a cell. | 2015-10-01 |
20150275209 | COMPOSITIONS AND METHODS FOR ENHANCING CANCER IMMUNOTHERAPY - The invention provides an isolated or purified CD8+? T cell which comprises an antigen-specific T cell receptor and an exogenous nucleic acid encoding a microRNA-155 (miR-155) molecule, and methods of preparing the same. The invention also provides a pharmaceutical composition comprising the CD8+ T cell a carrier. Further provided is a method for treating or preventing a medical condition, such as cancer, by adoptively transferring to a mammal an amount of the CD8+? T cells effective to treat or prevent the medical condition. | 2015-10-01 |
20150275210 | TREATMENT OF METASTATIC BREAST CANCER - As described herein, increased expression of microRNA-708 reduces migration and metastasis of cancer cells. | 2015-10-01 |
20150275211 | ANTISENSE COMPOUNDS AND USES THEREOF - The present invention provides compounds comprising oligonucleotides complementary to a pyruvate kinase M transcript. Certain such compounds are useful for hybridizing to a pyruvate kinase M transcript, including but not limited to a pyruvate kinase M transcript in a cell. In certain embodiments, such hybridization results in modulation of splicing of the pyruvate kinase M transcript. In certain embodiments, such compounds are used to treat one or more symptoms associated with cancer. | 2015-10-01 |
20150275212 | Oligonucleotide Conjugates - The invention relates to the field of oligonucleotide therapeutics, and in particular to the use of a cleavable, e.g. a phosphodiester region covalently attached to a conjugate, a targeting group or blocking group to enhance the properties of the oligonucleotides, for example to improve the therapeutic index. | 2015-10-01 |
20150275213 | METHODS AND COMPOSITIONS INVOLVING MIRNA AND MIRNA INHIBITOR MOLECULES - The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. | 2015-10-01 |
20150275214 | DELIVERY OF dsRNA TO ARTHROPODS - The invention is to methods of gene silencing in arthropods using dsRNA. The method is include contacting the arthropod with, and/or directly feeding the arthropod, the dsRNA to the arthropods to deliver the dsRNA to arthropod tissues. It is envisaged that the methods of the invention will have use in determining the biological function of genes in arthropods. Methods of pest control of arthropods, and of protecting arthropods against parasites and predators are provided. Transgenic arthropods expressing dsRNA molecules are also provided by the present invention. | 2015-10-01 |
20150275215 | NEUTRALIZING RNA APTAMERS AGAINST PDGFBETA AND USES THEREOF IN THE THERAPY AND DIAGNOSIS OF HYPERPROLIFERATIVE DISEASES - Nuclease-resistant RNA aptamers are provided which are capable of neutralizing PDGFRβ and are therefore useful in the diagnosis and/or therapy of PDGFRβ-associated and hyperproliferative-associated diseases, such as cancer and primary tumour metastasis. RNA aptamers provided herein include a modified synthetic RNA sequence wherein at least one pyrimidine residue is modified to 2′-fluoropyrimidine. Pharmaceutical compositions and diagnostic kits comprising RNA aptamers are also provided. | 2015-10-01 |
20150275216 | METHODS AND COMPOSITIONS FOR INHIBITION OF IMMUNE RESPONSES AND AUTOIMMUNITY - The application relates to the use of immunoregulatory polynucleotides and/or immunoregulatory compounds in combination with other therapeutic agents. The application further relates to immunoregulatory polynucleotides and/or immunoregulatory compounds comprising a modified immunoregulatory sequence. It also relates to the administration of the immunoregulatory polynucleotides and/or immunoregulatory compounds comprising a modified immunoregulatory sequence to regulate an immune response. | 2015-10-01 |
20150275217 | NOVEL RIG-I LIGANDS AND METHODS FOR PRODUCING THEM - The present invention relates to new triphosphate-modified oligonucleotides which may act as RIG-I ligands as well as a new method allowing the synthesis and purification in high yield and purity suitable for pharmaceutical applications. | 2015-10-01 |
20150275218 | Organic Compositions to Treat Beta-ENaC-Related diseases - The present disclosure relates to RNAi agents useful in methods of treating BetaENaC-related diseases such as cystic fibrosis, pseudohypoaldosteronism type 1 (PHA1), Liddle's syndrome, hypertension, alkalosis, hypokalemia, and obesity-associated hypertension, using a therapeutically effective amount of a RNAi agent to Beta-ENaC. | 2015-10-01 |
20150275219 | ENGINEERING OF MULTI-CARBON SUBSTRATE UTILIZATION PATHWAYS IN METHANOTROPHIC BACTERIA - The present disclosure relates to genetically engineered methanotrophic bacteria with the capability of growing on a multi-carbon substrate as a primary or sole carbon source and methods for growing methanotrophic bacteria on a multi-carbon substrate. | 2015-10-01 |
20150275220 | CARBOXYLATED POLYAMINE DERIVATIVES AS TRANSFECTION REAGENTS - The invention provides polyamine derivatives, a use of the polyamine derivatives for the transfection of polyanions into cells, and a method of transfecting cells with a polyanion, comprising mixing said polyanion with a polyamine derivative e.g. in a buffer and treating said cells with the mixture obtained in the previous step. | 2015-10-01 |
20150275221 | REPLICATIVE MINICIRCLE VECTORS WITH IMPROVED EXPRESSION - The present invention relates to the production and use of covalently closed circular (ccc) recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly to vector modifications that improve expression of said DNA molecules. | 2015-10-01 |
20150275222 | CRZ1 MUTANT FUNGAL CELLS - The present invention provides CRZ1 mutant fungal host cells, such as | 2015-10-01 |
20150275223 | Enhanced Acyltransferase Polynucleotides, Polypeptides and Methods of Use - The invention provides modified DGAT1 proteins that are modified in the N-terminal region upstream of the acyl-Co A binding site. The modified DGAT proteins show enhanced activity, without reduced protein accumulation when expressed in cells. The modified DGAT1 proteins of the invention can be expressed in cells to increase cellular lipid accumulation and/or modify the cellular lipid profile. The invention also provides polynucleotides encoding the modified DGAT1 proteins, cells and compositions comprising the polynucleotides or modified DGAT proteins, and methods using the modified DGAT1 proteins to produce oil. | 2015-10-01 |
20150275224 | PLANTS CONTAINING A HETEROLOGOUS FLAVOHEMOGLOBIN GENE AND METHODS OF USE THEREOF - Plant nitrogen use efficiency in corn has been improved by transformation with a flavohemoglobin gene. Plants comprising a flavohemoglobin gene have decreased nitric oxide (NO) levels, increased biomass accumulation under a sufficient nitrogen growth condition, and increased chlorophyll content under a limiting nitrogen growth condition. Additionally, these transformed plants evidence higher levels of yield. | 2015-10-01 |
20150275225 | PLANTS HAVING ALTERED AGRONOMIC CHARACTERISTICS UNDER NITROGEN LIMITING CONDITIONS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING LNT5 POLYPEPTIDES AND HOMOLOGS THEREOF - Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering agronomic characteristics of plants under nitrogen limiting conditions compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a LNT5 polypeptide. | 2015-10-01 |
20150275226 | COTTON VARIETY FM 1320GL - The cotton variety FM 1320GL is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 1320GL with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of FM 1320GL and to plants of FM 1320GL reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 1320GL. | 2015-10-01 |
20150275227 | COTTON VARIETY ST 4747GLB2 - The cotton variety ST 4747GLB2 is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety ST 4747GLB2 with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of ST 4747GLB2 and to plants of ST 4747GLB2 reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from ST 4747GLB2. | 2015-10-01 |
20150275228 | Compositions and Methods Conferring Resistance of Maize to Corn Rootworm I - Methods and compositions for increasing a plant's resistance to an insect pest such as the corn rootworm are provided. Methods are provided for overexpression of Crw1, or variants thereof, in a host plant or plant cell to increase resistance to an insect pest in a plant such as maize. Moreover, methods are provided for identifying variants of Crw1 that when incorporated into a plant via transgenic or traditional breeding means increase resistance to an insect pest in a plant such as maize. | 2015-10-01 |
20150275229 | SELF-ITERATING EXOSOMAL VECTOR FOR EFFECTIVE BIOMOLECULAR TRANSFERS - The present invention provides for a process for transferring biomolecules such as polynucleotides and protein from cell to cell, eventually resulting in the transport of a biomolecular cargo throughout the entirety of one or more of a cell culture, tissue, organ, organ system, or organism. | 2015-10-01 |
20150275230 | METHOD FOR ESTABLISHING MODIFIED HOST CELL - An object of the present invention is to establish a cell line that is useful as a host cell for use in recombinant protein production, highly expresses transgenes stably, and grows stably. | 2015-10-01 |
20150275231 | Method of Preventing or Reducing Virus Transmission in Animals - The subject invention provides materials and methods for improving animal resistance to infection by intestinal viruses. This is accomplished by interfering with intestinal virus uptake employing methods that (1) reduce virus binding to receptors in the intestinal lining; (2) introduce decoy receptors expressed in the mammary gland leading to decoy secretion in milk; (3) produce decoy receptors by a variety of protein synthesis methods to provide decoy receptors to non-genetically modified animals, including humans; and/or (4) administer a vector to a non-genetically modified animal which vector has been genetically modified to produce a decoy receptor. | 2015-10-01 |
20150275232 | Site-Specific Serine Recombinases and Methods of Their Use - The present invention provides a method for obtaining site-specific recombination in a eukaryotic cell, the method comprising providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site; contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination between the recombination attachment sites, wherein the recombinase polypeptide can mediate recominbination between the first and second recombination attachment sites, the first recombination attachment site is a phage genomic recombination attachment site (attP) or a bacterial genomic recombination attachment site (attB), the second recombination site is attB or attP, and the recombinase is selected from the group consisting of a | 2015-10-01 |
20150275233 | METHOD OF PRODUCING ISOPRENE MONOMER - A host cell includes a heterogeneous expression unit including: (a) a polynucleotide encoding a mevalonate kinase derived from a microorganism belonging to a genus selected from | 2015-10-01 |
20150275234 | BIOGAS PROCESS WITH NUTRIENT RECOVERY - The invention is a method of two-phase anaerobic digestion where monitoring and adjusting the nitrogen status (carbon to nitrogen molar ratio, i.e. C/N molar ratio or total or ammoniacal nitrogen content) enables maintaining optimum conditions during the process. The method improves the use of a variety of feedstock materials or facilitates monodigestion of one feedstock. Especially the introduction of nitrogen rich feedstock materials in the process is amended. A community of hydrolyzing and acidogenic microorganisms in the first phase digester performs ammonification i.e. release of organic nitrogen as ammonia. Nitrogen and phosphorus are removed and recovered from the digestate which then undergoes biogasification in the second phase of the process. Reject water from biogasification can be recycled within the process. | 2015-10-01 |
20150275235 | CELLS WITH IMPROVED PENTOSE CONVERSION - The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity. | 2015-10-01 |
20150275236 | METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE - A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order, wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA. | 2015-10-01 |
20150275237 | PROCESSES FOR CO-PRODUCING XYLITOL WITH ETHANOL OR OTHER FERMENTATION PRODUCTS - What is disclosed is a biorefining process to co-produce xylitol with ethanol or other products. In some variations, a process for producing ethanol and xylitol from lignocellulosic biomass, comprises: extracting hemicelluloses from lignocellulosic biomass, wherein the hemicelluloses include xylose oligomers and other sugar oligomers; hydrolyzing the xylose oligomers and the other sugar oligomers, using an acid catalyst or enzymes, to generate xylose and other sugar monomers, respectively; fermenting the other sugar monomers to ethanol using a suitable ethanol-producing microorganism; removing at least some of the ethanol (to increase concentration of xylose); fermenting the xylose to xylitol using a suitable xylitol-producing microorganism; and recovering the xylitol at high concentration. | 2015-10-01 |
20150275238 | GENETICALLY ENGINEERED MICROBES AND METHODS FOR CONVERTING ORGANIC ACIDS TO ALCOHOLS - Disclosed herein are genetically engineered microbes. In one embodiment, a genetically engineered microbe includes a metabolic pathway for the production of an alcohol from an organic acid. For instance, a genetically engineered microbe converts acetate, butyrate, propionate, isobutyrate, valerate, isovalerate, caproate, or phenylacetate, to Ethanol, Butanol, Propanol, Isobutanol, 1-Pentanol, Isoamylalcohol, 1-Hexanol, Phenylethanol, respectively. Also provided herein are methods of using the microbes. | 2015-10-01 |
20150275239 | GENETICALLY ENGINEERED BACTERIAL CELL HAVING ENHANCED ACTIVITY OF GlnD OR GlnK AND METHOD OF PRODUCING ORGANIC ACID BY USING THE SAME - A genetically engineered bacteria cell having an enhanced activity of GlnD or GlnK, and a method of producing succinic acid by using the genetically engineered bacteria cell are provided. | 2015-10-01 |
20150275240 | METHODS AND MATERIALS FOR CULTIVATION AND/OR PROPAGATION OF A PHOTOSYNTHETIC ORGANISM - The present disclosure provides methods and materials for the cultivation and/or propagation of a photosynthetic organism. Such methods may comprise the use of a lamp assembly that comprises a plurality of circuit boards, each comprising at least three edges, arranged in a substantially spherical shape defining an interior lamp assembly volume, wherein the plurality of circuit boards comprise a first planar surface in contact with the interior lamp assembly volume and an opposing second planar surface comprising light emitting diodes (LEDs); and a barrier that surrounds the plurality of circuit boards forming the substantially spherical shape. | 2015-10-01 |
20150275241 | POLYHYDROXYALKANOATE PRODUCTION METHODS AND MATERIALS AND MICROORGANISMS USED IN SAME - Embodiments of the invention relate generally to methods to generate microorganisms and/or microorganism cultures that exhibit the ability to produce polyhydroxyalkanoates (PHA) from carbon sources at high efficiencies. In several embodiments, preferential expression of, or preferential growth of microorganisms utilizing certain metabolic pathways, enables the high efficiency PHA production from carbon-containing gases or materials. Several embodiments relate to the microorganism cultures, and/or microorganisms isolated therefrom. | 2015-10-01 |
20150275242 | MICROORGANISMS AND METHODS FOR PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS AND RELATED COMPOUNDS - The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid. | 2015-10-01 |
20150275243 | RECOMBINANT ORGANISMS - The invention relates to genetically modified organisms with enhanced production of omega-3 long chain polyunsaturated fatty acids. | 2015-10-01 |
20150275244 | EICOSAPENTAENOIC ACID-PRODUCING MICROORGANISMS, FATTY ACID COMPOSITIONS, AND METHODS OF MAKING AND USES THEREOF - The invention is directed to microbial oils containing omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid, eicosapentaenoic acid, and optionally docosapentaenoic acid and dosage forms containing such oils. | 2015-10-01 |
20150275245 | BIOTECHNOLOGICAL PRODUCTION OF ALCOHOLS AND DERIVATIVES THEREOF - The present invention relates to a microorganism having a reduced fatty acid degradation capacity and expressing a recombinant alkane oxidase, a method for oxidizing an alkyl, comprising a contacting the alkyl with an aqueous solution comprising the inventive cell. | 2015-10-01 |
20150275246 | Method for Producing L-Amino Acid - A method for producing of an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, which has been modified so that the acpP-fabF operon is attenuated, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium. | 2015-10-01 |
20150275247 | MEANS AND METHODS FOR THE ENZYMATIC PRODUCTION OF L-METHIONINE FROM O-PHOSPHO-L-HOMOSERINE AND METHANETHIOL - Provided is a method for producing L-methionine in which O-phospho-L-homoserine and methanethiol are enzymatically converted into L-methionine and H3PO4. Such a conversion is achieved by an enzyme called O-phospho-L-homoserine (OHPS) dependent methionine synthase. Also described are O-phospho-L-homoserine (OHPS) dependent methionine synthases, i.e. proteins which are able to enzymatically convert O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4 as well as microorganisms which have been genetically modified so as to be able to produce L-methionine from O-phospho-L-homoserine and methanethiol. Furthermore described are methods to screen for enzymes that catalyse the conversion of O-phospho-L-homoserine and methanethiol into L-methionine and H | 2015-10-01 |
20150275248 | GENE CLUSTER FOR BIOSYNTHESIS OF CORNEXISTIN AND HYDROXYCORNEXISTIN - The invention pertains to the field of production of natural products and, in particular, in the field of production of cornexistin and hydroxycornexistin. It provides polynucleotides encoding polypeptides involved in the biosynthesis of cornexistin and hydroxycornexistin as well as vectors and recombinant microorganisms comprising such polynucleotides. Also provided are methods for the production of natural products, in particular methods for the production of cornexistin and hydroxycornexistin, using such polynucleotides and polpeptides encoded therein, as well as vectors and recombinant microorganisms comprising such polynucleotides and polypeptides. | 2015-10-01 |
20150275249 | PROCESS FOR PRODUCING CHIRAL 1 - SUBSTITUTED 2 - PIPERIDINOLS EMPLOYING OXIDOREDUCTASES - The present invention relates to an enantioselective enzymatic process for the preparation of an optically active 3-hydroxypiperidinecarboxylic acid derivative. | 2015-10-01 |
20150275250 | THERMOPHILIC AND THERMOACIDOPHILIC BIOPOLYMER DEGRADING GENES AND ENZYMES FROM ALICYCLOBACILLUS ACIDOCALDARIUS AND RELATED ORGANISMS, METHODS - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 2015-10-01 |
20150275251 | METHODS OF BIOSYNTHESIZING BACTERIAL EXTRACELLULAR GALACTOMANNAN POLYSACCHARIDES AND SUBUNITS THEREOF FOR USE IN SUBTERRANEAN FORMATION OPERATIONS - Methods of biosynthesizing bacterial extracellular galactomannan polysaccharides for use in subterranean formation operations such as drilling operations, fracturing operations, or gravel packing operations including providing a feedstock comprising mannose and galactose; providing bacteria effective at fermenting mannose and galactose; introducing the bacteria to the feedstock; and fermenting the bacteria so as to produce an extracellular galactomannan polysaccharide. | 2015-10-01 |
20150275252 | PRODUCTION OF FERMENTABLE BIOMASS SUGARS USING HIGH-SOLIDS ENZYMATIC HYDROLYSIS - In some variations, this invention provides a process for producing fermentable sugars from cellulosic biomass, comprising: extracting biomass with steam or hot water to produce an extract liquor containing hemicellulose oligomers, dissolved lignin, and cellulose-rich solids; separating and washing the cellulose-rich solids; removing a portion of glucan contained in the washed cellulose-rich solids as glucose oligomers using a liquefaction-focused blend of enzymes; co-hydrolyzing glucose oligomers and hemicellulose oligomers, with enzymes or an acid catalyst, to produce glucose and hemicellulose monomers; and recovering the glucose and hemicellulose monomers as fermentable sugars. The liquefaction-focused blend of enzymes contains endoglucanases and exoglucanases. A rotating unit for high-solids enzymatic hydrolysis may be employed, with continuous liquid removal. Optionally, the glucose and the hemicellulose monomers may be recovered as separate streams. The residual cellulose (not hydrolyzed) as well as the lignin may be recovered and combusted, or utilized for other purposes. | 2015-10-01 |
20150275253 | METHOD FOR PRODUCING INDIGESTIBLE DEXTRIN - The present invention provides a method for producing indigestible dextrin which is inexpensive and can simply and efficiently suppress coloring. The method for producing indigestible dextrin includes the steps of liquefying pyrodextrin and saccharifying pyrodextrin, wherein at least one of the liquefying step and the saccharifying step is performed in the presence of activated carbon. | 2015-10-01 |
20150275254 | ENZYME LOADING, CELLULOSE HYDROLYSIS, AND INHIBITION OF CELLOBIOHYDROLASES USING LIQUID HOT WATER PRETREATMENT - Disclosed herein are methods for extracting sugars from cellulose-containing sources to achieve high glucose yields while greatly reducing the amount of cellulase enzyme needed. | 2015-10-01 |
20150275255 | Endoglucanase For Reducing The Viscosity Of A Plant Materials Slurry - The present disclosure relates to composition comprising EG cellulase and methods of use, thereof. The compositions are useful, e.g., for reducing the viscosity of plant material slurry. | 2015-10-01 |
20150275256 | PRODUCTION OF GLUCAN POLYMERS FROM ALTERNATE SUCROSE SOURCES - Reaction solutions are disclosed herein comprising water, incompletely refined sucrose, and a glucosyltransferase enzyme that synthesizes insoluble poly alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages and a weight average degree of polymerization (DP | 2015-10-01 |
20150275257 | Nucleic Acid Amplification - The present invention provides improved methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products. | 2015-10-01 |
20150275258 | NUCLEIC ACID AMPLIFICATION REACTION DEVICE AND NUCLEIC ACID AMPLIFICATION METHOD - A nucleic acid amplification reaction device includes a rotary body on which a cartridge is mountable, the cartridge including a tube having, in this order in the inside thereof, a third plug formed of oil, a fourth plug formed of an elution solution which causes the nucleic acid to be eluted from the magnetic particles bound to the nucleic acid, and a fifth plug formed of oil, and a nucleic acid amplification reaction container which communicates with the side of the tube on which the fifth plug is disposed, and a heater which is able to heat the end portion of the tube on the side communicating with the nucleic acid amplification reaction container and a first region as the end portion of the nucleic acid amplification reaction container on the side communicating with the tube. | 2015-10-01 |
20150275259 | METHOD FOR PREVENTING REDUCTION OF POLYPEPTIDE BY ADDING AMINO ACID TO CULTURE SOLUTION - The present invention provides a method for preventing the reduction of a desired polypeptide during purification in order to purify the polypeptide in high yield from a culture solution which contains recombinant host cells and the polypeptide. | 2015-10-01 |
20150275260 | METHOD FOR THE PRODUCTION OF POLYPEPTIDES IN THE PERIPLASM OF PROKARYOTIC CELLS - Herein is reported a method for producing a polypeptide comprising the step of incubating (resuspended) prokaryotic cells in a solution comprising about 10 mM to about 95 mM Tris-HCl and about 2 mM to about 6 mM EDTA at a pH value of about 7 to about 10 for about 15 min to about 6 h at about 25° C. | 2015-10-01 |
20150275261 | FORMATION OF IMMOBILIZED BIOLOGICAL LAYERS FOR SENSING - The invention is directed to enzyme immobilization compositions comprising: one or more enzymes, a humectant, an acrylic-based monomer, a water-soluble organic photo-initiator and a water-soluble acrylic-based cross-linker in a substantially homogeneous aqueous mixture. The invention is also directed to methods for forming sensors comprising such compositions and to apparati for forming arrays of immobilized layers on an array of sensors by dispensing such compositions onto a substrate. | 2015-10-01 |
20150275262 | AUTOMATED QUANTIFICATION OF MICROORGANISM GROWTH PARAMETERS THROUGH TEMPORALLY RESOLVED MICROSCOPIC IMAGING - A method of high-density, multiparameter growth analysis founded upon modeling microcolony expansion on solid media is described. The method extracts the key growth parameters (lag time, doubling time, carrying capacity and viability) that together define microcolony growth from seeded cells. The invention relates to a method to determine growth parameters of cells that is scalable, time-resolved and quantitative. | 2015-10-01 |
20150275263 | FLOW CYTOMETRY-BASED SYSTEMS AND METHODS FOR DETECTING MICROBES - In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein. | 2015-10-01 |
20150275264 | FLOW CYTOMETRY-BASED SYSTEMS AND METHODS FOR DETECTING MICROBES - In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein. | 2015-10-01 |
20150275265 | METHODS FOR DIAGNOSING BACTERIAL INFECTIONS - The present invention is directed to methods of determining the presence or absence of a bacterial infection in a patient using isotopically-labeled tyrosine and/or isotopically-labeled p-hydroxyphenylacetic acid. | 2015-10-01 |
20150275266 | ANALYSIS OF DISULFIDE BONDS - The present disclosure relates to methods of evaluating, identifying, and/or producing (e.g., manufacturing) pharmaceutical products (e.g., protein therapeutics). In some instances, methods herein allow highly resolved evaluation of the disulfide bond profiles of protein therapeutics. | 2015-10-01 |
20150275267 | METHOD AND KIT FOR PREPARING A TARGET RNA DEPLETED SAMPLE - The present invention provides a method of preparing a target RNA depleted composition from an initial RNA containing composition, comprising a) contacting the initial RNA containing composition with one or more groups of probe molecules, wherein a group of probe molecules has the following characteristics: i) the group comprises two or more different probe molecules having a length of 100 nt or less; ii) the probe molecules comprised in said group are complementary to a target region of a target RNA; iii) when hybridized to said target region, the two or more different probe molecules are located adjacent to each other in the formed double-stranded hybrid; and generating a double-stranded hybrid between the target RNA and the probe molecules; b) capturing the double-stranded hybrid by using a binding agent which binds the double-stranded hybrid, thereby forming a hybrid/binding agent complex; c) separating the hybrid/binding agent complexes from the composition, thereby providing a target RNA depleted composition. By combining hybrid capturing with a unique probe design, an improved depletion method is provided which effectively and specifically removes unwanted target RNA such as ribosomal RNA (rRNA) from total RNA, while ensuring recovery of mRNA and noncoding RNA from various species, including human, mouse, and rat. By improving the ratio of useful data, decreasing bias, and preserving non-coding RNA species, the method provides high-quality RNA that is especially suited for next-generation sequencing (NGS) applications. By integrating said depletion method in common sequencing applications, in particular NGS applications such as transcriptome sequencing, improved methods for sequencing RNA molecules are provided. | 2015-10-01 |
20150275268 | STABILISATION OF BIOLOGICAL SAMPLES - The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample and for stabilizing the transcriptome of contained cells. | 2015-10-01 |
20150275269 | METHOD FOR PURIFYING NUCLEIC ACID AND KIT - The present invention discloses a method for purifying nucleic acids and a kit. In particular, the present invention discloses a reagent combination for purifying nucleic acids from a specimen containing nucleic acids, a kit made based on the reagent combination, and a method for purifying nucleic acids using the reagent combination or the kit. | 2015-10-01 |
20150275270 | COMPOSITIONS AND METHODS FOR THE ANALYSIS OF DEGRADED NUCLEIC ACIDS - The invention relates to compositions and methods for gene expression analysis. In some embodiments, the invention provides compositions and methods for amplifying targets in a degraded nucleic acid sample. In some embodiments, the invention provides methods for determining the quality of nucleic acids (e.g., the degree of degradation) in a nucleic acid sample. The invention also provides methods for producing a gene expression profile from a degraded RNA sample. | 2015-10-01 |
20150275271 | PLASMA TREATMENT FOR DNA BINDING - The invention provides a composition including DNA bonded to a plasma-treated surface, the plasma can be any suitable plasma, such as an argon plasma, a compressed air plasma, a flame-based plasma or a vacuum plasma. Surfaces treatable by the methods of the invention include ceramic, metal, fabric and organic polymer surfaces. The DNA can be any DNA, such as a marker DNA, which can be linear or circular, single-stranded or double stranded and from about 25 bases to about 10,000 bases in length. Also provided is a method of binding DNA to a surface, including the steps of exposing the surface to a plasma to produce a plasma-treated surface; and applying DNA to the plasma-treated surface to produce surface bound DNA on the treated surface. A system for binding DNA to a surface is also disclosed, the system includes a plasma generator adapted to treating a surface with a plasma to produce a plasma-treated surface; and an applicator containing DNA adapted to applying DNA to the plasma-treated surface to produce surface bound DNA on the plasma-treated surface. | 2015-10-01 |
20150275272 | BIOCHIP - A biochip; a liquid which has a different specific gravity from that of the reaction mixture and is immiscible with the reaction mixture; and an additive containing, as a principal component, a carbinol-modified silicone resin, a carboxyl-modified silicone resin, an amino-modified silicone resin, a polyether-modified silicone resin, a silanol-modified silicone resin, or a fluoro-modified silicone resin, wherein the vessel comprising, a flow channel that is capable of flowing a liquid droplet of a reaction mixture containing a surfactant in the longitudinal direction of the vessel. | 2015-10-01 |
20150275273 | NUCLEIC ACID ANALYSIS DEVICE AND NUCLEIC ACID ANALYSIS METHOD - A nucleic acid analysis device includes a mounting portion supporting a nucleic acid amplification reaction container, first and second heaters which heat first and second portions of the container, a rotating mechanism which collectively rotates the container and heaters, a heater controller, a rotating mechanism controller, a fluorescence measurer, and a nucleic acid melting curve analyzer. The container is repeatedly inverted while a nucleic acid amplification reaction cycle occurs therein. The first and second heaters heat the container such that the first portion reaches a first temperature and the second portion reaches a second temperature while the nucleic acid amplification reaction cycle occurs. After the nucleic acid amplification reaction cycle ends, the second heater increases the temperature of the second portion. The fluorescence emitted from the amplified nucleic acid is measured while the temperature of the second portion increases. A nucleic acid melting curve is obtained from the fluorescence. | 2015-10-01 |
20150275274 | DETECTION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS - The present methods pertain to amplifying and/or detecting | 2015-10-01 |
20150275275 | PROGNOSTIC OF DIET IMPACT ON OBESITY-RELATED CO-MORBIDITIES - The present invention relates to a method for determining whether an overweight subject has a reduced gut bacterial diversity. The said method comprises detecting the presence or absence in a gut DNA sample of at least one gene of at least one bacterial species of Table 1 or Table 2, respectively. | 2015-10-01 |
20150275276 | PCR REACTION MIXTURES AND METHODS OF USING SAME - Provided herein are methods and compositions involving Polymerase Chain Reaction (PCR). | 2015-10-01 |
20150275277 | DETECTION OF GENE FUSIONS BY INTRAGENIC DIFFERENTIAL EXPRESSION (IDE) USING AVERAGE CYCLE THRESHOLDS - Described herein are methods and kits for detecting the presence or absence of gene dysregulations such as those arising from gene fusions and/or chromosomal abnormalities, e.g. translocations, insertions, inversions and deletions. The methods, compositions and kits are useful for detecting mutations that cause the differential expression of a 5′ portion of a target gene relative to the 3′ region of the target gene. The average expression of the 5′ portion of the target gene is compared with the average expression of the 3′ portion of the target gene to determine an intragenic differential expression (IDE). The IDE can then be used to determine if a dysregulation or a particular disease (or susceptibility to a disease) is present or absent in a subject or sample. | 2015-10-01 |
20150275278 | MODIFIED THYMINE POLYNUCLEOTIDE OLIGOMERS AND METHODS - Disclosed are modified thymine bases that provide enhanced base-pairing affinity for adenine or 2,6-diaminopurine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified thymine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection. | 2015-10-01 |
20150275279 | RNA-BASED, AMPLIFICATION-FREE, ORGANISM IDENTIFICATION USING NANO-ENABLED ELECTRONIC DETECTION - A technique that uses nanotechnology to electrically detect and identify RNA sequences without the need for using enzymatic amplification methods or fluorescent labels. The technique may be scaled into large multiplexed arrays for high-throughput and rapid screening. The technique is further able to differentiate closely related variants of a given bacterial or viral species or strain. This technique addresses the need for a quick, efficient, and inexpensive bacterial and viral detection and identification system. | 2015-10-01 |
20150275280 | Devices, Compositions and Methods Pertaining To Microscopic Analysis Of Microorganisms and Other Analytes of Interest - This invention pertains to devices, compositions and methods that can be used for the rapid determination of microorganisms, cells and other analytes of interest (e.g. a nucleic acid target) as well as associated properties of said microorganisms, cells and analytes. For example, said devices, compositions and/or methods can be applied to the determination of a trait of a microorganism present in a sample. Said devices, compositions and methods utilize matrix-forming prolonged-dissolution hydrophilic polymer to encapsulate hybridization probes and optionally other assay reagents within two or more reagent zones and/or matrix zones disposed on the surface of a substrate. Each reagent zone and/or matrix zone can be designed as a separate assay. Thus, a plurality of assays can be performed on a single substrate. In some embodiments, devices can be supplied in a form ready for a customer to rapidly perform one or a plurality of assays. | 2015-10-01 |
20150275281 | INSTANTANEOUS DETECTION OF BIOMARKS AND USES THEREOF - The present invention relates to a novel method for detecting instantaneously a biomarker immobilized to a solid surface and related uses. The method comprises exposing the biomarker to a probe having a magnetic label in a solution; applying a magnetic field to the solution, whereby a complex of the biomarker and the probe is formed on the solid surface; withdrawing the magnetic field; removing the solution from the solid surface; and detecting the complex instantaneously, wherein the presence of the complex on the solid surface indicates the presence of the biomarker. | 2015-10-01 |
20150275282 | ISOTHERMAL AMPLIFICATION UNDER LOW SALT CONDITION - Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (T | 2015-10-01 |
20150275283 | MODIFIED CYTOSINE POLYNUCLEOTIDE OLIGOMERS AND METHODS - Disclosed are modified cytosine bases that provide enhanced base-pairing affinity for guanine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified cytosine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection. | 2015-10-01 |
20150275284 | CLONAL AMPLIFICATION OF NUCLEIC ACID ON SOLID SURFACE WITH TEMPLATE WALKING - Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided. | 2015-10-01 |
20150275285 | COMPOSITIONS AND METHODS OF NUCLEIC ACID PREPARATION AND ANALYSES - The present invention provides methods of generating single-stranded polynucleotides comprising use of adaptor sequence(s), single-stranded polynucleotide amplification and a primer comprising BJSA. Methods of analysing one or more regions on a desired polynucleotide using probes and single-stranded polynucleotides are also provided. Also provided are kits and compositions useful for these methods. | 2015-10-01 |