39th week of 2015 patent applcation highlights part 24 |
Patent application number | Title | Published |
20150267163 | METHOD FOR INDUCING BACTERIA TO ENTER INTO VIABLE BUT NONCULTURABLE STATE - A method induces bacteria to enter into viable, but nonculturable state. The method includes subjecting a target bacterium to high pressure carbon dioxide treatment so as to make it enter into the viable, but nonculturable state, which can be demonstrated experimentally. The viable but nonculturable state can be entered quickly, such as within one hour. | 2015-09-24 |
20150267164 | THREE-DIMENSIONAL CELL CULTURING - The present invention is related to methods and materials for culturing and transporting stem cells in a three-dimensional culture. The materials comprise cellulose nanofibrils in a form of three-dimensional discontinuous entities in a biocompatible hydrogel. | 2015-09-24 |
20150267165 | METHOD FOR GENERATING PRIMATE TROPHOBLASTS - The first method to cause a culture of human and other primate stem cells to directly and uniformly differentiate into a committed cell lineage is disclosed. Treatment of primate stem cells with a single protein trophoblast induction factor causes the cells to transform into human trophoblast cells, the precursor cells of the placenta. Several protein factors including bone morphogenic protein 4 (BMP4), BMP2, BMP7, and growth and differentiation factor 5 can serve as trophoblast-inducting factors. | 2015-09-24 |
20150267166 | FGF HAVING ENHANCED STABILITY - Methods are provided that exploit thermostable FGF-1 proteins for support of human pluripotent stem cell cultures. Also provided are compositions containing thermostable FGF-1 for culturing of human pluripotent stem cells. | 2015-09-24 |
20150267167 | MULTIPOTENT ADULT STEM CELLS AND METHODS FOR ISOLATION - The invention provides isolated stem cells of non-embryonic origin that can be maintained in culture in the undifferentiated state or differentiated to form cells of multiple tissue types. Also provided are methods of isolation and culture, as well as therapeutic uses for the isolated cells. | 2015-09-24 |
20150267168 | CELLULAR TEST SYSTEMS FOR THE DETERMINATION OF THE BIOLOGICAL ACTIVITIES OF NEUROTOXIN POLYPEPTIDES - The present invention pertains to a method for the generation of neurotoxin-sensitive, neuronal differentiated cells comprising the steps of: a) cultivating tumor cells which are able to differentiate into neuronal cells in a culture medium under conditions and for a time which primes said tumor cells for neuronal differentiation; and b) cultivating the tumor cells primed for neuronal differentiation of a) in a differentiation medium having an osmolality of 100 to 270 mOsm/kg, and comprising (i) B27 supplement and/or (ii) N2 supplement, for at least 3 days, thereby obtaining neurotoxin-sensitive, neuronal differentiated cells. The invention further relates to neurotoxin-sensitive, neuronal differentiated cells obtainable by the method of the invention. In addition, the invention encompasses a method for determining the activity of a neurotoxin polypeptide comprising the steps of: a) contacting the neurotoxin-sensitive, neuronal differentiated cells obtainable by the method of the invention with a neurotoxin polypeptide; b) cultivating the neurotoxin-sensitive, neuronal differentiated cells of step a) for 3 to 74 hours or 72 hours under conditions which allow for the neurotoxin polypeptide to exert its biological activity; and c) determining the activity of the neurotoxin polypeptide in the said cells after cultivation according to step b). Finally, the invention provides for a medium comprising OptiMEM, FBS, B27 supplement, and N2 supplement. | 2015-09-24 |
20150267169 | CELLS EXHIBITING NEURONAL PROGENITOR CELL CHARACTERISTICS AND METHODS OF MAKING THEM - Disclosed are cells exhibiting neuronal progenitor cell characteristics, and methods of making them from marrow adherent stem cells by regulating cellular pathways in the marrow adherent stem cells that are associated with glial transdifferentiation of the marrow adherent stem cells. | 2015-09-24 |
20150267170 | Renovation and Repopulation of Decellularized Tissues and Cadaveric Organs by Stem Cells - A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed. | 2015-09-24 |
20150267171 | ISOLATION OF ADULT MULTIPOTENTIAL CELLS BY TISSUE NON-SPECIFIC ALKALINE PHOSPHATASE - The present invention relates to the use of tissue non-specific alkaline phosphatase (TNAP) as a marker for identifying and/or isolating adult multipotential cells. The present invention also relates to cell populations enriched by methods of the present invention and therapeutic uses of these cells. | 2015-09-24 |
20150267172 | CULTURE MEDIUM ADDITIVE FOR USE IN SERUM-FREE CULTURING OF ANIMAL CELL, KIT AND USE THEREOF - Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium. | 2015-09-24 |
20150267173 | METHOD FOR ACTIVATING OCT4 FOR INDUCTION OF PLURIPOTENT STEM CELLS - The invention relates to a method for inducing pluripotent stem cells, wherein a mammalian cell is contacted with a compound characterized by a general formula 1 wherein R | 2015-09-24 |
20150267174 | REPROGRAMMING PEPTIDE AND USE THEREOF - The present invention provides a production method of a pluripotent spheroid cell, comprising introducing a reprogramming peptide containing a nuclear localization signal into a differentiated cell; a preparation for induction of a pluripotent spheroid cell from a differentiated cell, which contains the reprogramming peptide, and the like. According to the present invention, a somatic cell can be reprogrammed without using reprogramming factors including transcription factors such as Oct3/4, Sox2 and the like, and low-molecular-weight compounds that promote reprogramming such as HDAC inhibitors and the like, and an induced pluripotent stem cell-like cell, a reprogrammed cell or a transdifferentiated cell can be obtained from a somatic cell. | 2015-09-24 |
20150267175 | Host Cells with Artificial Endosymbionts - The present invention is directed generally to eukaryotic host cells comprising artificial endosymbionts and methods of introducing artificial endosymbionts into eukaryotic host cells. The invention provides artificial endosymbionts that introduce a phenotype to host cells that is maintained in daughter cells. The invention additionally provides eukaryotic host cells containing magnetotactic bacteria. | 2015-09-24 |
20150267176 | TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR (TALE) - LYSINE-SPECIFIC DEMETHYLASE 1 (LSD1) FUSION PROTEINS - Fusion proteins comprising a DNA binding domain, e.g., a TAL effector repeat array (TALE) or zinc finger array, and a catalytic domain comprising a sequence that catalyzes histone demethylation, and methods of use thereof. | 2015-09-24 |
20150267177 | NOVEL METHANOL DEHYDROGENASE ENZYMES FROM BACILLUS - The present invention relates to a nucleic acid molecule, which encodes a polypeptide having alcohol dehydrogenase activity, in particular methanol dehydrogenase activity, comprising having a nucleotide sequence selected from the group consisting of: (i) a nucleotide sequence as set forth in any one of SEQ ID NOs: 1 (mdh2-MGA3), 3 (mdh3-MGA3), or 5 (mdh2-PB1); (ii) a nucleotide sequence having at least 90% sequence identity, more particularly at least 91, 92, 93, 94, 95, 96, 97, 98 or 99% sequence identity, with a nucleotide sequence as set forth in any one of SEQ ID NOs: 1, 3 or 5; (iii) a nucleotide sequence which is degenerate with any one of the nucleotide sequences of SEQ ID NOs: 1, 3 or 5; (iv) a nucleotide sequence which is a part of the nucleotide sequence of any one of SEQ ID NOs: 1, 3 or 5, or of a nucleotide sequence which is degenerate with a sequence of SEQ ID NOs: 1, 3 or 5; (v) a nucleotide sequence encoding all or part of a polypeptide whose amino acid sequence is set forth in any one of SEQ ID NOs: 2 (Mdh2-MGA3), 4 (Mdh3-MGA3) or 6 (Mdh2-PB1); and (vi) a nucleotide sequence encoding all or part of a polypeptide which has an amino acid sequence having at least 90% sequence identity, preferably at least 91, 92, 93, 94, 95, 96, 97, 98 or 99% sequence identity, with an amino acid sequence as set forth in any one of SEQ ID NOs: 2, 4 or 6; or a nucleic acid molecule comprising a nucleotide sequence which is complementary to the nucleotide sequence of any one of (i) to (vi). Also provided are recombinant constructs, vectors and host cells comprising such a nucleic acid molecule and polypeptides encoded thereby. Such molecules may advantageously be used in the genetic modification of host cells, for example to introduce or modify methanol dehydrogenase activity. | 2015-09-24 |
20150267178 | PROTEIN HAVING FLAVIN ADENINE DINUCLEOTIDE-DEPENDENT GLUCOSE DEHYDROGENASE ACTIVITY - The present invention provides a protein derived from a thermophilic filamentous fungus having flavin adenine dinucleotide-dependent glucose dehydrogenase activity. The present invention provides an enzyme for glucose measurement that has better thermal stability than that of conventional enzymes while being unaffected by dissolved oxygen, not requiring the addition of a coenzyme, and maintaining the excellent properties of FADGDH in terms of having excellent substrate specificity (particularly low reactivity with maltose). | 2015-09-24 |
20150267179 | Polypeptides Having Peroxygenase Activity - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from | 2015-09-24 |
20150267180 | HPPD VARIANTS AND METHODS OF USE - Compositions and methods for conferring herbicide tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. | 2015-09-24 |
20150267181 | Phosphodeoxyribosyl Transferase Mutant Enzymes and Uses Thereof - The present invention relates to enzyme mutants obtained from the nucleoside deoxyribosyltransferase (NDT) of | 2015-09-24 |
20150267182 | NEW DNA POLYMERASES WITH INCREASED SUBSTRATE SCOPE - The present invention relates to DNA polymerases displaying increased substrate scope such as improved reverse transcriptase and DNA polymerase activities, as well as improved activities for incorporating and extending modified nucleotides. In particular, the present invention relates to DNA polymerases derived from wild-type | 2015-09-24 |
20150267183 | Alpha-amylase variant with altered properties - The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase. | 2015-09-24 |
20150267184 | Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains. | 2015-09-24 |
20150267185 | STREPTOCOCCUS BACTERIOPHAGE LYSINS FOR DETECTION AND TREATMENT OF GRAM POSITIVE BACTERIA - The present invention provides methods, compositions and articles of manufacture useful for the prophylactic and therapeutic amelioration and treatment of gram-positive bacteria, including | 2015-09-24 |
20150267186 | METHOD FOR DELIVERING AGENTS INTO CELLS USING BACTERIAL TOXINS - The invention provides compositions and methods for delivering a bioactive moiety comprising at least one non-natural component into a cell cytosol of a eukaryotic cell. The bioactive moiety is linked to an A component of a bacterial toxin, a functional wild-type or modified fragment thereof, or an A component surrogate or mimetic. For delivery, the cell is contacted with the linked bioactive moiety and a corresponding B component of the bacterial toxin or a functional fragment thereof. | 2015-09-24 |
20150267187 | Designer Ubiquitin Ligases for Regulation of Intracellular Pathogenic Proteins - The present invention relates to a designer or recombinant ubiquitin ligase molecule that includes an antibody fragment that is specific for a toxin active fragment, wherein the toxin active fragment is an enzymatically active fragment of one or more toxins or toxin serotypes; and an E3-ligase domain that comprises an E3-ligase or polypeptide that facilitates E2-mediated ubiquitination of the toxin active fragment. In an embodiment, the composition further includes a delivery system that allow the designer ubiquitin ligase to enter the cell. The present invention further includes methods for treating an individual intoxicated with a toxin by administering the designer ubiquitin ligase of the present invention. | 2015-09-24 |
20150267188 | TARGET SUBSTANCE PURIFICATION DEVICE, NUCLEIC ACID PURIFICATION DEVICE, TARGET SUBSTANCE GENERATING METHOD, AND NUCLEIC ACID AMPLIFICATION METHOD - A target substance purification device includes: a capillary that is elongated in a longitudinal direction and includes a first plug of oil, a plug of an elution liquid that undergoes phase separation from oil and elutes the target substance from a magnetic body detachably retaining the target substance, and a second plug of oil inside the capillary; a magnetic force applicator that applies a magnetic force to the capillary to retain and control the movement of the magnetic body along the longitudinal direction of the capillary; and a liquid sending mechanism that moves the elution liquid in the longitudinal direction of the capillary while the magnetic force applicator restricts movement of the magnetic body along the longitudinal direction of the capillary. | 2015-09-24 |
20150267189 | METHODS AND PRODUCTS FOR EXPRESSING PROTEINS IN CELLS - The present invention relates in part to nucleic acids encoding proteins, therapeutics comprising nucleic acids encoding proteins, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods and products for altering the DNA sequence of a cell are described, as are methods and products for inducing cells to express proteins using synthetic RNA molecules. Therapeutics comprising nucleic acids encoding gene-editing proteins are also described. | 2015-09-24 |
20150267190 | TRANSLATIONAL ENHANCER-ELEMENT DEPENDENT VECTOR SYSTEMS - A translation enhancer-driven positive feedback vector system is disclosed which is designed to facilitate identification of a Translational Enhancer Element (TEE) and to provide a means for overexpression of gene products. The system exploits both transcriptional and translational approaches to control the expression levels of genes and/or gene products. Methods are also disclosed for screening libraries of random nucleotide sequences to identify translational elements and for overproduction of proteins, which have uses in both research and industrial environments. | 2015-09-24 |
20150267191 | COMPOSITIONS AND METHODS FOR LABELING OF AGENTS - The invention provides, inter alia, methods for uniquely labeling populations of agents of interest using random combinations of oligonucleotides. The oligonucleotides may comprise a unique nucleotide sequence and/or one or more non-nucleic acid detectable moieties. | 2015-09-24 |
20150267192 | NUCLEASE RESISTANT POLYNUCLEOTIDES AND USES THEREOF - The invention provides, among other things, methods of mRNA stabilizing mRNA and nuclease resistant mRNA prepared in accordance with such methods. In certain embodiments, the nuclease resistant mRNA encodes a functional protein, such as enzyme, and is characterized by its resistance to nuclease digestion, increased half-life and/or its ability to produce increased amounts of the functional protein (e.g., enzyme) encoded thereby. | 2015-09-24 |
20150267193 | MicroRNAs and Methods of Using Same - MicroRNAs (miRs) and methods and uses thereof for modulating Wnt signaling pathways are described herein. More particularly, miRs and compositions thereof and methods for treating a cancer associated with an elevated or hyper-activated Wnt signaling pathway using same, as well as miRs for use in treating cancers associated with an elevated or hyper-activated Wnt signaling pathway and for use in the preparation of a medicament for treating a cancer associated with an elevated or hyper-activated Wnt signaling pathway in a subject are described herein | 2015-09-24 |
20150267194 | DOUBLE-STRANDED OLIGONUCLEOTIDE MOLECULES TO DDIT4 AND METHODS OF USE THEREOF - Provided herein are double stranded nucleic acid molecules, compositions comprising same and methods of use thereof for the treatment of a subject wherein expression of DDIT4 is associated with the etiology or progression of a disease or disorder in the subject. The compounds are preferably chemically synthesized and modified dsRNA molecules. | 2015-09-24 |
20150267195 | OLIGOMERIC COMPOUNDS COMPRISING BICYCLIC NUCLEOSIDES AND USES THEREOF - The present invention provides oligomeric compounds. Certain such oligomeric compounds are useful for hybridizing to a complementary nucleic acid, including but not limited, to nucleic acids in a cell. In certain embodiments, hybridization results in modulation of the amount activity or expression of the target nucleic acid in a cell. In certain embodiments, the present invention provides compounds comprising oligonucleotides. In certain embodiments, such oligonucleotides comprise a region having a gapmer sugar motif. In certain embodiments, oligonucleotides comprise one or more type of modified sugar moieties and/or naturally occurring sugar moieties arranged along an oligonucleotide or region thereof in a defined pattern or sugar modification motif. | 2015-09-24 |
20150267196 | BIODEGRADABLE HYDROGEL FOR POLYNUCLEOTIDE DELIVERY - A composition includes a biodegradable hydrogel that includes a hydrogel forming base polymer and a plurality of physiologically degradable ester linkages, amide linkages, azide-alkyne cycloaddition linkages, acrylate-thiol linkages, urethane linkages, and/or methacrylate-thiol linkages, a polynucleotide coupling polymeric molecule; and a polynucleotide. The polynucleotide is released under physiological conditions in a spatial and/or temporally controlled or predetermined manner from the composition. | 2015-09-24 |
20150267197 | METHODS FOR MODULATING C9ORF72 EXPRESSION - Disclosed herein are methods for reducing expression of C90RF72 mRNA and protein in an animal with C90RF72 specific inhibitors. Such methods are useful to treat, prevent, or ameliorate neurodegenerative diseases in an individual in need thereof. Such C90RF72 specific inhibitors include antisense compounds. Examples of neurodegenerative diseases that can be treated, prevented, and ameliorated with the administration C90RF72 specific inhibitors include amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), corticalbasal degeneration syndrome (CBD), atypical Parkinsonian syndrome, and olivopontocerellar degeneration (OPCD). | 2015-09-24 |
20150267198 | METHODS FOR TREATING APOLIPOPROTEIN E4-ASSOCIATED DISORDERS - The present disclosure provides a method of increasing the functionality of a GABAergic interneuron in the hilus of the hippocampus of an individual having at least one apolipoprotein E4 (apoE4) allele. The method generally involves reducing tau levels in the interneuron. | 2015-09-24 |
20150267199 | METHODS AND COMPOSITIONS FOR MODULATING ANGIOGENESIS - The present invention provides compositions comprising antisense nucleic acids that reduce miR-126 levels in an endothelial cell. The present invention provides compositions comprising a target protector nucleic acid. The present invention provides methods of modulating angiogenesis in an individual, the methods generally involving administering to the individual an effective amount of an agent that increases or that decreases the level of miR-126 in endothelial cells of the individual. | 2015-09-24 |
20150267200 | RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA) - The present invention concerns methods and reagents useful in modulating gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to synthetic chemically modified small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating RNA interference (RNAi) against target nucleic acid sequences. The small nucleic acid molecules are useful in the treatment of any disease or condition that responds to modulation of gene expression or activity in a cell, tissue, or organism. | 2015-09-24 |
20150267201 | IMMUNOSTIMULATORY PLASMIDS - The present invention relates to immunomodulator compositions and methods of use as well as methods of making. The immunomodulator compositions comprise immunostimulatory plasmids, or DNA sequence, capable of eliciting an immune response in a recipient subject. Further, the immunostimulatory plasmids, or DNA sequence, do not contain antibiotic resistance coding sequence to help reduce the potential of horizontal transfer of antibiotic resistance in a population. | 2015-09-24 |
20150267202 | ANTISENSE ANTIVIRAL COMPOUND AND METHOD FOR TREATING ss/RNA VIRAL INFECTION - The invention provides antisense antiviral compounds and methods of their use and production in inhibition of growth of viruses of the Flaviviridae, Picornaviridae, Caliciviridae, Togaviridae, Arteriviridae, Coronaviridae, Astroviridae and Hepeviridae families in the treatment of a viral infection. The antisense antiviral compounds are substantially uncharged morpholino oligonucleotides having a sequence of 12-40 subunits, including at least 12 subunits having a targeting sequence that is complementary to a region associated with stem-loop secondary structure within the 5′-terminal end 40 bases of the positive-sense RNA strand of the virus. | 2015-09-24 |
20150267203 | RNA SILENCING IN ANIMALS AS AN ANTIVIRAL DEFENSE - The present invention provides recombinant DNA constructs for inactivation of viral or endogenous genes in a cell, wherein the construct comprises viral sequence sufficient to activate RNA silencing. In another aspect, the invention provides methods for identifying RNA silencing suppressors by sequence analysis and functional tests. In yet another aspect, the invention provides a method for identifying inhibitors of RNA silencing suppressors. In still other aspects, the invention comprises methods for identifying genes in the antiviral RNA silencing pathway, enhancers of the antiviral pathway, and methods of treating or preventing viral infections using enhancers of the pathway. | 2015-09-24 |
20150267204 | TREATMENT OF SIRTUIN (SIRT) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO A SIRTUIN (SIRT) - The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of a Sirtuin (SIRT), in particular, by targeting natural antisense polynucleotides of a Sirtuin (SIRT). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of Sirtuins (SIRT)s. | 2015-09-24 |
20150267205 | METHODS AND COMPOSITIONS FOR REGULATION OF ZINC FINGER PROTEIN EXPRESSION - The present disclosure is in the field of genome engineering, particularly regulating targeted modification of the genome. | 2015-09-24 |
20150267206 | MULTIPROTEIN EXPRESSION CASSETTES - Methods for expressing multiple proteins by constructing transformation vectors that include multiprotein expression cassettes and transforming hosts with vectors and by engineering hosts expressing multiprotein units are provided. Multiprotein units that include multiple proteins fused to modified inteins capable of effecting splicing of the multiprotein units are described. Expression cassettes that include nucleic acids encoding multiprotein units and hosts including the expression cassettes are also provided. | 2015-09-24 |
20150267207 | METHODS OF HOST CELL MODIFICATION - Described herein are novel methods of inserting nucleic acid sequences into host cells. Also described herein are genetically stable host cells comprising inserted nucleic acid sequences and methods of using such host cells in the generation of proteins. | 2015-09-24 |
20150267208 | PURIFICATION PROCESS OF NASCENT DNA - A method for initiating the replication of a deoxyribonucleic acid molecule includes inserting into the DNA at least one nucleic acid molecule representing a multicellular DNA replication origin. The replication origin contains at least nine nucleotides and contains at least three uninterrupted origin repeating elements (ORE), each ORE having the sequence N | 2015-09-24 |
20150267209 | System and Method for Visualization of Optimized Protein Expression - The present disclosure describes a novel recombinant gene expression system and a method of its use. The recombinant gene expression system is adapted to enable a user to monitor the successful expression of a protein in real time by allowing a small portion (e.g., less than 10%) of the target protein to be expressed as a fusion with a readily detectable reporter. The recombinant gene expression system further allows a user to select a clone that exhibits high, medium or low expression by providing a random ribosomal binding site having the nucleotide sequence N | 2015-09-24 |
20150267210 | ELECTROCOMPETENT CELLS AND PREPARATION THEREOF - The present invention provides a method of preparing electrocompetent cells from Gram-negative bacteria characterized in that the bacteria are prepared under room temperature. Also provided are electrocompetent Gram-negative bacteria and kits which comprise the electrocompetent bacteria. | 2015-09-24 |
20150267211 | METHODS OF PRODUCING 6-CARBON CHEMICALS VIA CoA-DEPENDENT CARBON CHAIN ELONGATION ASSOCIATED WITH CARBON STORAGE - This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6; backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria. | 2015-09-24 |
20150267212 | PICHIA PASTORIS STRAINS FOR PRODUCING PREDOMINANTLY HOMOGENEOUS GLYCAN STRUCTURE - Disclosed herein are novel | 2015-09-24 |
20150267213 | STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY - strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the | 2015-09-24 |
20150267214 | PLASTID TRANSFORMATION USING LINEAR DNA VECTORS - The present disclosure provides methods of plastid transformation using linear DNA vectors and plant tissues having substantially non-degraded plastid DNA. Also provided are linear DNA vectors useful for the methods provided herein, transplastomic plants or plant parts obtained by the methods provided herein, and progenies of such transplastomic plants or plant parts. | 2015-09-24 |
20150267215 | SOYBEAN GAPD PROMOTER AND ITS USE IN CONSTITUTIVE EXPRESSION OF TRANSGENIC GENES IN PLANTS - The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean eukaryotic glyceraldehyde-3-phosphate dehydrogenasegene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a constitutive manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same. | 2015-09-24 |
20150267216 | PROCESSES FOR PRODUCING HYDROCARBON PRODUCTS - The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels. | 2015-09-24 |
20150267217 | GENES INVOLVED IN ASYMMETRIC CELL DIVISION - Described are methods of isolating genes involved in the process of asymmetric cell division. Further disclosed are genes isolated with this method, and their use in controlling root formation, preferably lateral root formation. | 2015-09-24 |
20150267218 | ISOLATED POLYNUCLEOTIDES, POLYPEPTIDES AND METHODS OF USING SAME FOR INCREASING ABIOTIC STRESS TOLERANCE, BIOMASS AND YIELD OF PLANTS - Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 529, 475-528, 530-770, 6179-9796, 9798-10421, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 314, 1-313, 315-474, 771-6178, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing abiotic stress tolerance, yield, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant. | 2015-09-24 |
20150267219 | HEAT-RESISTANCE RICE GENE OSZFP, SCREENING MARKER AND SEPARATION METHOD THEREOF - Provided in the present invention are a separated endogenous rice gene resistant to high temperature (hereinafter referred to as the OsZFP gene for short) and a polypeptide encoded thereby, optimizing rice cells comprising the heat-resistant gene of the present invention or the polypeptide encoded thereby, and the plant cell preparation method thereof. Further provided are new methods and technologies for breeding new varieties of heat-resistant crops, comprising the related regulatory sequence for heat-resistance and a closely linked molecular marker denoting the heat-resistant gene and the sequence thereof. | 2015-09-24 |
20150267220 | Maize RING-H2 Genes and Methods of Use - The present invention relates to the field of plant molecular biology, more particularly to the regulation of genes that increase drought tolerance and yield. Provided herein are methods finding use in agriculture for increasing drought tolerance in dicot and monocot plants. Methods comprising introducing into a plant cell a polynucleotide that encodes a maize XERICO polypeptide operably linked to a promoter that drives expression in a plant are provided. Methods are further provided for maintaining or increasing yield in plants under drought conditions by introducing into a plant cell a polynucleotide encoding a maize XERICO poly-peptide and a polynucleotide encoding an abscisic acid (ABA)-associated polypeptide. Also provided are transformed plants, plant tissues, plant cells, and seeds thereof. | 2015-09-24 |
20150267221 | TRANSGENIC MAIZE EVENT MON 87419 AND METHODS OF USE THEREOF - The invention provides recombinant DNA molecules that are unique to the maize MON 87419 event and transgenic maize plants, plant parts, seeds, cells, and agricultural products containing the MON 87419 event as well as methods of using and detecting the maize MON 87419 event. Transgenic maize plants containing the MON 87419 event exhibit tolerance to dicamba and glufosinate herbicides. | 2015-09-24 |
20150267222 | Methods and Compositions for Plant Pest Control - The present invention provides methods and compositions to improve fungal disease resistance and/or nematode resistance in various crop plants. The present invention also provides for combinations of compositions and methods to improve fungal disease resistance and/or nematode resistance in various crop plants. Powdery mildews are fungal diseases that affect a wide range of plants including cereals, grasses, vegetables, ornamentals, weeds, shrubs, fruit trees, broad-leaved shade and forest trees, that is caused by different species of fungi in the order Erysiphales. | 2015-09-24 |
20150267223 | METHODS AND COMPOSITIONS FOR REGULATING HIV INFECTION - Methods and compositions for regulating HIV infection and/or replication in which an anti-HIV transgene is integrated into the genome of a cell using a nuclease. | 2015-09-24 |
20150267224 | Biosynthesis of 1-Alkenes in Engineered Microorganisms - Various 1-alkenes, including 1-nonadecene and 1-octadecene, are synthesized by the engineered microorganisms and methods of the invention. In certain embodiments, the microorganisms comprise recombinant 1-alkene synthases. The engineered microorganisms may be photosynthetic microorganisms such as cyanobacteria. | 2015-09-24 |
20150267225 | PROCESSES AND SYSTEMS FOR THE PRODUCTION OF FERMENTATION PRODUCTS - The present invention relates to processes and systems for the production of fermentation products such as alcohols. The present invention also provides methods for separating feed stream components for improved biomass processing and productivity. | 2015-09-24 |
20150267226 | RECOMBINANT MICROORGANISM WITH ABILITY TO PRODUCE GLYCEROL, 3-HP, OR ACRYLIC ACID AND METHOD OF PRODUCING GLYCEROL, 3-HP, OR ACRYLIC ACID BY USING THE SAME - A recombinant microorganism having the ability to produce glycerol 3-HP, or acrylic acid, in which glycerol is produced from dihydroxyacetone phosphate (DHAP) via dihydroxyacetone (DHA) in a biosynthetic pathway, and a method of producing glycerol, 3-hydroxypropioninc acid (3-HP), or acrylic acid by using the recombinant microorganism. | 2015-09-24 |
20150267227 | Vanillin Synthase - The invention relates to methods for producing vanillin and related compounds. The methods involve use of a vanillin synthase capable of catalyzing side chain cleavage of ferulic acid to form vanillin. The invention also relates to host organisms expressing such vanillin synthases useful in the methods. | 2015-09-24 |
20150267228 | GENETICALLY ENGINEERED YEAST - A genetically modified | 2015-09-24 |
20150267229 | COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS - The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric 4-hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium. | 2015-09-24 |
20150267230 | RECOMBINANT ENZYME SYSTEMS FOR EFFICIENT PRODUCTION OF ITACONATE IN CELLS - Described herein is a fusion polypeptide containing an aconitase and a cis-aconitate decarboxylase. Also described are a genetically modified cell expressing the fusion polypeptide and a method of using the cell to produce itaconate. | 2015-09-24 |
20150267231 | BIOTECHNOLOGICAL 2-HYDROXYISOBUTYRIC ACID PRODUCTION - The present invention relates to a knallgas bacterium or acetogenic bacterium expressing a 2-hydroxyisobutyryl-coenzyme A mutase, a method for the production of 2-hydroxyisobutyric acid, comprising contacting in an aqueous medium the knallgas bacterium or acetogenic with a gas mixture comprising hydrogen and carbon dioxide and the use of the knallgas bacterium or acetogenic bacterium for the production of 2-hydroxyisobutyric acid. | 2015-09-24 |
20150267232 | IN VIVO AND IN VITRO CARBENE INSERTION AND NITRENE TRANSFER REACTIONS CATALYZED BY HEME ENZYMES - This invention relates to the use of heme-containing enzymes to catalyze carbene and nitrene insertion and transfer reactions with greater selectivity, mild reaction conditions, and convenient production. | 2015-09-24 |
20150267233 | COMPOSITIONS AND METHODS FOR PRODUCING BENZYLISOQUINOLINE ALKALOIDS - The present invention relates to host cells that produce compounds that are characterized as benzylisoquinolines, as well as select precursors and intermediates thereof. The host cells comprise one, two or more heterologous coding sequences wherein each of the heterologous coding sequences encodes an enzyme involved in the metabolic pathway of a benzylisoquinoline, or its precursors or intermediates from a starting compound. The invention also relates to methods of producing the benzylisoquinoline, as well as select precursors and intermediates thereof by culturing the host cells under culture conditions that promote expression of the enzymes that produce the benzylisoquinoline or precursors or intermediates thereof. | 2015-09-24 |
20150267234 | BRANCHED ALPHA-GLUCAN, ALPHA-GLUCOSYLTRANSFERASE WHICH FORMS THE GLUCAN, THEIR PREPARATION AND USES - The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched α-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows:
| 2015-09-24 |
20150267235 | Method of Producing Glycolipids - A protein selected from the following (a) to (c), a gene encoding the protein, a transformant in which the gene is subjected to deletion, mutation or repression of gene expression, and a method of producing a glycolipid using the transformant: | 2015-09-24 |
20150267236 | NOVEL USE OF GLUCOSYLTRANSFERASE GENE - The isolation and characterization of a glucosyltransferase gene from | 2015-09-24 |
20150267237 | CELL CULTURE COMPOSITIONS AND METHODS FOR POLYPEPTIDE PRODUCTION - Cell culture media, such as chemically defined cell culture media, are provided, as are methods of using the media for cell growth (i.e., cell culture) and polypeptide (e.g., antibody) production. Compositions comprising polypeptides produced by the methods are also provided. | 2015-09-24 |
20150267238 | Cell Culture Medium And Process For Protein Expression, Said Medium And Process Comprising A Pam Inhibitor - The present invention is related to a cell culture medium for the expression of a protein, which medium comprises a PAM inhibitor, or a physiological equivalent thereof, and to a cell culture process for the expression of a protein, in which process a PAM inhibitor, or a physiological equivalent thereof, is used (FIG. | 2015-09-24 |
20150267239 | Biosensor Chip Having Precise Count Function and Method of Sensing Amount of Cells - Disclosure is a biosensor chip having precise count function comprising: a substrate, a plurality of ground wire waveguide layers, a signal waveguide layer and a protective layer. Wherein, the plurality of ground wire waveguide layers are located on two sides of the substrate, the signal waveguide layer is located on the substrate and between the plurality of ground wire waveguide layers, wherein the signal waveguide layer has a recess which forms a cell sensing region; and the protective layer covers a portion of the ground wire waveguide layers and a portion of the signal waveguide layer to expose the recess. | 2015-09-24 |
20150267240 | IDENTIFICATION AND ANALYSIS OF FETAL TROPHOBLAST CELLS IN CERVICAL MUCUS FOR PRENATAL DIAGNOSIS - A method of retrieving fetal cells from an endocervical sample by removing the mucus from the endocervical sample by disassociating fetal cells and maternal cells in the endocervical sample; and isolating disassociated fetal cells from other cells in the endocervical sample. Also provided is a method of retrieving fetal cells from an endocervical sample, by obtaining a mixture of disassociated cells prepared by the above method, treating the cells with a fetal-specific antibody, identifying cells that have bound to the fetal-specific antibody, and isolating the identified cells. The disassociated cell prepared by the above method can be analyzed and used for a variety of purposes including, but not limited to, the identification of fetal cells among cervical cells, determination of fetal cell density to predict high risk pregnancy, genetic analysis of fetal cells, and determination of growth factor or other biomarker expression to predict obstetrical disorders. | 2015-09-24 |
20150267241 | METHOD FOR IDENTIFYING COMPOUND FOR INHIBITING AN ACTIVITY OF A HISTONE LYSINE DEMETHYLASE - The present invention relates to a method for identifying a compound that inhibits an activity of a histone lysine demethylase. | 2015-09-24 |
20150267242 | ACYL-COA DEHYDROGENASE ASSAYS - A method for conducting medium-chain acyl-CoA dehydrogenase (MCAD) and very-long-chain acyl-CoA dehydrogenase (VCAD) enzymatic activity assays is provided. The method may include, but is not limited to, preparing a sample; preparing an enzyme-specific substrate/reagent mixture; mixing an aliquot of the prepared sample with an aliquot of the enzyme-specific substrate/reagent mixture; reading absorbance in the range of about 600 nm; incubating the prepared sample and enzyme-specific substrate/reagent mixture; and reading absorbance in the range of about 600 nm at various time intervals. | 2015-09-24 |
20150267243 | INDUCIBLE RECONSTITUTION AND REAL-TIME QUANTITATIVE KINETIC SYSTEM FOR THE STUDY OF INTRAMEMBRANE ENZYMES - The present invention relates to the field of enzymes. More specifically, the present invention provides an inducible reconstitution and real-time quantitative kinetic system for the study of intramembrane enzymes. In a specific embodiment, a method of screening for modulators of an intramembrane protease comprises the steps of (a) contacting in a mixture the protease and a substrate with a lipid under acidic or basic conditions to form a membrane comprising the lipid bilayer, protease and the substrate; (b) contacting a test agent with the membrane mixture; (c) adjusting the pH to physiological conditions; (d) assaying substrate cleavage by the protease; and (e) comparing the assayed substrate cleavage to a reference that does not include the test agent, wherein an increase or a decrease of substrate cleavage by the protease relative to the reference identifies the test agent as a modulator of the intramembrane protease. | 2015-09-24 |
20150267244 | Method for Detecting Endotoxins and/or 1,3- -D-Glucans in a Sample - Detection of endotoxins and/or 1,3-β-D-glucans in a sample includes making contact with the sample using an amoebocyte lysate and at least one type of particles having a surface made of at least one metal or metalloid oxide. This makes it possible to considerably increase the sensitivity of lysate detection. | 2015-09-24 |
20150267245 | PRESERVATION OF BIOLOGICAL MATERIALS IN NON-AQUEOUS FLUID MEDIA - The invention provides compositions and methods for preserving a biological material—such as a protein, a nucleic acid or a biological sample, or any combination thereof—in a substantially water-free, nonionic or ionic organic solvent. Improved preservation, including for example the stability and/or the solubility of the biological material in the substantially water-free fluid medium, is achieved with compositions comprising one or more substances (e.g., an antioxidant) described in the disclosure, and/or a metal salt. The biological material is soluble and stable, and retains its function and activity, when it is preserved in the substantially water-free fluid medium at ambient temperature or higher for extended periods of time. Therefore, the composition comprising the biological material does not need to be refrigerated or frozen during shipping or storage. | 2015-09-24 |
20150267246 | MICROFLUIDIC PROCESS FOR TREATING AND ANALYSING A SOLUTION CONTAINING A BIOLOGICAL MATERIAL AND CORRESPONDING MICROFLUIDIC CIRCUIT - The subject matter of the present invention is a microfluidic process for treating and analysing a solution containing a biological material, comprising a step of introducing the solution into microchannels of a microfluidic circuit ( | 2015-09-24 |
20150267247 | Microdroplet Formation by Wells in a Microfluidic Device - A method of forming microdroplets is provided that includes forming a well plate, using lithography, where the well plate includes a microchannel and a microwell in a surface of the fluid channel, flowing a first fluid into the microchannel, where the microchannel and the microwell are filled with the first fluid, and flowing a second fluid into the microchannel, where the first fluid is displaced from the microchannel, where the first fluid remains in the microwell, where a microdroplet of the first fluid is formed. | 2015-09-24 |
20150267248 | INTEGRATED DEVICE FOR REAL TIME QUANTITATIVE PCR - A method for real-time quantitative detection of single-type, target nucleic acid sequences amplified using a PCR in a microwell, comprising introducing in the microwell a sample comprising target nucleic acid sequences, magnetic primers, and labelling probes; performing an amplification cycle to form labelled amplicons; attracting the magnetic primers to a surface through a magnetic field to form a layer including labelled amplification products and free magnetic primers; and detecting the labelled amplification products in the layer with a surface-specific reading method. | 2015-09-24 |
20150267249 | DETERMINATION OF REDUCED GUT BACTERIAL DIVERSITY - The present invention relates to a method for determining whether a subject has a reduced gut bacterial diversity. This method comprises the step of determining the presence or absence in a gut DNA sample of at least one gene from at least one bacterial species from Table 1 or Table 2, respectively. | 2015-09-24 |
20150267250 | COMPOSITIONS AND METHODS FOR DIAGNOSING COLON DISORDERS - The present invention relates to methods and compositions for diagnosing, monitoring, prognosticating, analyzing, etc., polymicrobial diseases. The present invention also relates to the microbial community present in the digestive tract and lumen in normal subjects, and subjects with digestive tract diseases, especially diseases of the colon, such as inflammatory bowel disease, including ulcerative colitis, Crohn's syndrome, and pouchitis. The present invention especially relates to compositions and methods for diagnosing and prognosticating the mentioned diseases and conditions, e.g., to determine the presence of the disease in a subject, to determine a therapeutic regimen, to determine the onset of active disease, to determine the predisposition to the disease, etc. | 2015-09-24 |
20150267251 | MULTIPLEX LABELING OF MOLECULES BY SEQUENTIAL HYBRIDIZATION BARCODING - The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. | 2015-09-24 |
20150267252 | PCR ASSAY FOR ANIMAL ORIGIN OF HEPARIN - The invention provides methods and reagents for use in distinguishing heparin sodium of porcine origin from heparin sodium of bovine origin using quantitative polymerase chain reaction. | 2015-09-24 |
20150267253 | MEMBRANE-INTEGRATED VIRAL DNA-PACKAGING MOTOR PROTEIN CONNECTOR BIOSENSOR FOR DNA SEQUENCING AND OTHER USES - Compositions and methods are disclosed that exploit the unprecedented modification of double-stranded DNA virus DNA-packaging motor protein connector polypeptides to render them capable of stable incorporation into lipid membranes as a self-assembled homodocamer that forms an aperture through which conductance can occur when an electrical potential is applied across the membrane. The aperture permits use of the modified protein as a biosensor, for dsDNA sequencing, SNP detection and highly sensitive affinity capture and fingerprinting of analytes, and also finds use in electropotential-driven solute translocation, such as for liposomal loading to form therapeutic nanoparticles (e.g., gene delivery) and bioreactors, and for other uses. The aperture can further be used in optical detection of dsDNA or other acceptor labeled analytes in a fluorophore donor labeled single pore channel. | 2015-09-24 |
20150267254 | MICRORNA PROFILES IN THE DIAGNOSIS OF MULTIPLE SCLEROSIS - The invention provides a method of determining if a patient is afflicted with multiple sclerosis (MS) using microRNA (miRNA) profiles of specific miRNAs that are present in the cerebrospinal fluid (CSF). This method can also be used to discriminate different forms of MS. The invention further comprises a kit for diagnosing or monitoring MS based upon the miRNA profiles according to the invention, and also relates to the use of said miRNA profiles in the diagnosis or monitoring of MS. | 2015-09-24 |
20150267255 | METHOD OF DETECTING CHROMOSOMAL ABNORMALITIES - The invention relates to a method of detecting chromosomal abnormalities, in particular, the invention relates to the diagnosis of fetal chromosomal abnormalities such as trisomy 21 (Down's syndrome) which comprises sequence analysis of cell-free DNA molecules in plasma samples obtained from maternal blood during gestation of the fetus. | 2015-09-24 |
20150267256 | METHOD FOR THE SIMULTANEOUS AMPLIFICATION OF A PLURALITY OF DIFFERENT NUCLEIC ACID TARGET SEQUENCES - The present invention relates to a method for the simultaneous amplification of a plurality of different nucleic acid target sequences comprising the steps of providing a plurality of different nucleic acid polymers as templates, each template comprising a specific target sequence and a primer annealing sequence located downstream of the target sequence, and amplifying the template by a polymerase dependent amplification reaction using a primer oligonucleotide comprising a primer sequence which is at least essentially complementary to the primer annealing sequence. The method is characterized in that for the polymerase dependent amplification reaction a set of primer oligonucleotides is used, said set comprising at least two primer oligonucleotides which are able to anneal to the primer annealing sequence of the same template and which differ from each other in the efficiency for the polymerase dependent amplification reaction to take place. | 2015-09-24 |
20150267257 | LEUKOCYTE MicroRNAS FOR USE IN DIAGNOSIS AND TREATMENT OF ENDOMETRIOSIS - Methods for diagnosis and treatment of endometriosis are described. Methods utilize the recognition that leukocyte miRNAs can be dramatically dysregulated subjects suffering from endometriosis. Accordingly, leukocyte miRNAs, as well as polynucleotides encoding the miRNAs can be utilized in the diagnosis and treatment of endometriosis. | 2015-09-24 |
20150267258 | BIOMARKERS FOR DETERMINING EFFECTIVE RESPONSE OF TREATMENTS OF HEPATOCELLULAR CARCINOMA (HCC) PATIENTS - This invention is directed to the use of one or more biomarkers defined as KRAS or NRAS gene for predicting the pharmaceutical efficacy or clinical response of MEK protein kinase inhibitor and/or Sorafenib or Regorafenib to be administered to a Hepatocellular carcinoma (HCC) patient. Further the invention is directed to in-vitro methods for identifying mutated-type KRAS or NRAS gene in HCC patient and kits thereof. | 2015-09-24 |
20150267259 | METHOD OF PROGNOSIS AND STRATIFICATION OF OVARIAN CANCER - A method for the prognosis of overall survival or prediction of therapeutic outcome for a patient suffering from epithelial ovarian cancer (EOC), comprising: a. providing a metabolism response sample from the patient, b. determining the expression level of microRNA family lethal-7b (let-7b) in the sample; c. using the expression level of the let-7b to obtain the prognosis of overall survival or prediction of therapeutic outcome for the patient. | 2015-09-24 |
20150267260 | NUCLEIC ACID AMPLIFICATION AND USE THEREOF - The invention features compositions and methods that are useful for the measurement of the quantity of a nucleic acid target in a sample. | 2015-09-24 |
20150267261 | PHOSPHOLIPASE C GAMMA 2 AND RESISTANCE ASSOCIATED MUTATIONS - Described herein is a mutation that confers resistance to the treatment with a BTK inhibitor. Described herein is a modified PLCγ2 polypeptide that is modified at amino acid position 742, 845, or 1140 and the modified PLCγ2 polypeptide exhibits decreased inhibition (e.g., resistance) to a covalent and/or irreversible BTK inhibitor. Described herein are diagnostic methods for detecting the modified polypeptide and nucleic acid encoding the modified polypeptide and applications of the methods thereof. Described herein are compositions, combinations, and kits containing the modified polypeptide and methods of using the modified polypeptide. Also described herein are methods of using the modified polypeptide as screening agents for the identification and design of inhibitors of PLCγ2. | 2015-09-24 |
20150267262 | APPLICATION OF QUANTUM DOTS FOR NUCLEAR STAINING - Embodiments of a system, method, and kit for visualizing a nucleus are disclosed. A tissue sample is pretreated with a protease to permeabilize the nucleus, and then incubated with a nanoparticle/DNA-binding moiety conjugate. The DNA-binding moiety includes at least one DNA-binding molecule. The conjugate binds to DNA within the nucleus, and the nanoparticle is visualized, thereby visualizing the nucleus. Computer and image analysis techniques are used to evaluate nuclear features such as chromosomal distribution, ploidy, shape, size, texture features, and/or contextual features. The method may be used in combination with other multiplexed tests on the tissue sample, including fluorescence in situ hybridization. Kits for performing the method include a protease enzyme composition, a nanoparticle/DNA-binding moiety conjugate, and a reaction buffer. | 2015-09-24 |