38th week of 2014 patent applcation highlights part 138 |
Patent application number | Title | Published |
20140272984 | Microfluidic Flow Monitoring - The present invention relates to systems and methods of monitoring velocity or flow in channels, especially in microfluidic channels. In some embodiments, the present invention relates to systems and methods of monitoring velocity or flow rate in systems and methods for performing a real-time polymerase chain reaction (PCR) in a continuous-flow microfluidic system. | 2014-09-18 |
20140272985 | Compositions and Methods for Intramolecular Nucleic Acid Rearrangement - Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a “reflex method”. In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided. | 2014-09-18 |
20140272986 | DIAGNOSTIC TEST FOR PREDICTING METASTASIS AND RECURRENCE IN CUTANEOUS MELANOMA - The invention as disclosed herein in encompasses a method for predicting the risk of metastasis of a primary cutaneous melanoma tumor, the method encompassing measuring the gene-expression levels of at least eight genes selected from a specific gene set in a sample taken from the primary cutaneous melanoma tumor; determining a gene-expression profile signature from the gene expression levels of the at least eight genes; comparing the gene-expression profile to the gene-expression profile of a predictive training set; and providing an indication as to whether the primary cutaneous melanoma tumor is a certain class of metastasis or treatment risk when the gene expression profile indicates that expression levels of at least eight genes are altered in a predictive manner as compared to the gene expression profile of the predictive training set. | 2014-09-18 |
20140272987 | GLATIRAMER ACETATE RESPONSE BIOMARKER mRNA POTENCY ASSAY - The invention describes assays for determining the potency of a test lot of glatiramer acetate (GA) by quantitating and comparing the levels of mRNA response biomarkers produced in mouse T-cells in response to stimulation with the test lot or a reference standard lot of GA, wherein the T-cells are obtained from mice immunized with the test lot or the reference standard lot of GA. Methods for identifying mRNA response biomarkers useful in the assays of the invention also are described. | 2014-09-18 |
20140272988 | TRANS-SPLICING TRANSCRIPTOME PROFILING - The present invention provides a method of identifying mRNA transcripts in the transcriptome of a cell comprising i) delivering into the cell a donor expression vector comprising nucleotides in a sequence encoding a trans-splicing barcode cassette, wherein the trans-splicing barcode cassette comprises a) a first portion, the nucleotide sequence of which encodes an intron comprising as part of its 3′ end, or followed at its 3′ end by a splice-site nucleotide sequence; followed at its 3′ end by, b) a second portion, the nucleotide sequence of which encodes a barcode polynucleotide; followed at its 3′ end by c) a third portion, which encodes a nucleotide identification element sequence, ii) exposing the cell to conditions such that the cell produces multiple copies of the trans-splicing barcode cassette encoded by the donor expression vector, which multiple copies of the trans-splicing barcode cassette each splice the barcode polynucleotide onto a mRNA transcript of the cell, thereby forming multiple mRNA transcripts of the cell, each spliced to the barcode polynucleotide; and iii) identifying the multiple mRNA transcripts that are spliced to the barcode polynucleotides, thereby identifying mRNA transcripts in the transcriptome of the cell. | 2014-09-18 |
20140272989 | REAGENT WELLS CONTAINING LYOPHILIZED REAGENTS - System, apparatuses, and methods for performing automated reagent-based analysis are provided. Also provided are methods for automated attachment of a cap to a reaction receptacle, and automated removal of a cap from a capped reaction receptacle. | 2014-09-18 |
20140272990 | SILICON AND GERMANIUM DYES FOR USE IN GENETIC IDENTITY - Si- and Ge-based dyes and methods of using same. | 2014-09-18 |
20140272991 | CALIBRATION METHOD, APPARATUS AND COMPUTER PROGRAM PRODUCT - Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The approach employs a single fixed data point in combination with a single adjustment calibrator amplified on the instrument that is to be calibrated for approximating a complete calibration curve. | 2014-09-18 |
20140272992 | Method and Apparatus for Rapidly and Cyclically Heating and Cooling a Fluid Sample During PCR Testing - Methods and apparatuses rapidly and cyclically heating and cooling a fluid sample during PCT testing to reduce the duration for full amplification of target DNA using most PCR procedures. Instead of heating and cooling static target solution in a reaction tube, the target solution reciprocates (alternately flows) back and forth within an elongate, thick-walled, small-bore tube. Sections of the tube are maintained at different temperatures so that an ascending/descending temperature gradient is maintained along the length of the tube. As the target solution flows within and along the tube from section to section, it rapidly achieves thermal equilibrium with the tube at each section, thereby rapidly thermally cycling the target solution. | 2014-09-18 |
20140272993 | METHOD OF SEQUENCING A FULL MICRORNA PROFILE FROM CEREBROSPINAL FLUID - The present invention provides methods of purifying RNA from a biological sample comprising two separate aqueous extractions of the organic phase obtained by mixing the biological sample with a first solution comprising guanidinium isothiocyanate and beta-mercaptoethanol and a second solution comprising phenol, chloroform, and isoamyl alcohol. Also provided are methods of sequencing RNA purified from a biological sample and methods of diagnosing Alzheimer's disease and Parkinson's disease in a subject by determining whether a plurality of miRNAs has deregulated expression in biological sample from the subject. | 2014-09-18 |
20140272994 | METHODS AND REAGENTS THAT SPECIFICALLY DETECT, DISTINGUISH AND QUANTIFY IFN-LAMBDA2 mRNA FROM IFN-LAMBDA3 mRNA IN HUMANS - The present invention provides a method of specifically detecting IFN-λ2 mRNA or IFN-λ3 mRNA. There is provided a qRT-PCR method specifically detecting, discriminating and quantifying IFN-λ2 and IFN-λ3 mRNA in a biological sample obtained from a human. There is provided qRT-PCR methods and primers and probes that specifically detect IFN-λ2 mRNA but not IFN-λ3 mRNA and vice versa in humans in order to detect, quantify and discriminate IFN-λ2 mRNA and IFN-λ3 mRNA. | 2014-09-18 |
20140272995 | SILICONE SURFACTANTS FOR EMULSION ASSAYS - System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals. | 2014-09-18 |
20140272996 | DROPLET GENERATOR WITH COLLECTION TUBE - A system, including methods and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include (1) a droplet generation component configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid, and (2) a droplet reservoir component configured to receive the droplet emulsion from the droplet generation component and then to be separated from the droplet generation component, so that subsequent assay steps may be conveniently performed using the droplet reservoir component. In some examples, the droplet reservoir component may be an industry standard PCR tube or a strip of interconnected PCR tubes. | 2014-09-18 |
20140272997 | Sample Preparation Device and Methods of Use - A device for isolating DNA from a sample containing cells, including a cartridge having an entrance port and an exit port, a membrane disposed between the entrance port and the exit port, and a plurality of channels between the membrane and the exit port. Additionally, systems and methods for isolating DNA from a sample containing cells and also systems and methods for amplifying and isolating single-stranded DNA from a sample containing DNA. | 2014-09-18 |
20140272998 | DIAGNOSTIC MICRORNA PROFILING IN CUTANEOUS T-CELL LYMPHOMA (CTCL) - The present invention relates to the field of cancer-diagnostics. In particular the invention relates to a microRNA expression signature that allows discriminating skin samples of cutaneous T-cell lymphomas (CTCL) from non-malignant (inflammantory) skin samples by use of quantitative polymerase chain reaction performed on reverse transcribed miRNA. miR-155, miR-326, miR-663b, miR-203 and miR-205 are shown to be differentially expressed. | 2014-09-18 |
20140272999 | PURIFICATION OF NUCLEIC ACID - The present invention relates to a simple and efficient method to isolate and purify nucleic acids, preferably genomic DNA, from complex samples compared with available methods, by using a ligand which relies on hydrogen bonding to purify the nucleic acids. Preferably the ligand is bound to magnetic beads/particles. More closely the method comprises adding a sample comprising nucleic acid to a polymer having neutral charge; reversibly binding said nucleic acid to said polymer by hydrogen bonding under pH conditions <5; washing said polymer; and eluting said nucleic acid from said polymer under conditions of pH >5. The method is very suitable for sample preparation of nucleic acids, for example for PCR applications. | 2014-09-18 |
20140273000 | METHODS FOR ANALYZING BLOOD TO DETECT DISEASES ASSOCIATED WITH ABNORMAL PROTEIN AGGREGATION - A method of detecting a disease associated with abnormal protein aggregation in a subject is provided, the method comprising (a) contacting leukocytes from the subject with a probe that binds to pathogenic protein aggregates, and (b) detecting the probe bound to the pathogenic protein aggregates, wherein the presence of pathogenic protein aggregates in the leukocytes is indicative that the subject has a disease associated with abnormal protein aggregation. In one embodiment, the disease is Alzheimer's disease or mild cognitive impairment. | 2014-09-18 |
20140273001 | FELINE BITTER TASTE RECEPTORS AND METHODS - A family of novel feline bitter taste receptors, referred to as feline TAS2R (fTAS2R), are disclosed herein. Isolated polynucleotides encoding the novel feline bitter taste receptors and chimeric polypeptides are also disclosed, as are expression vectors and host cells for expression of the novel feline bitter taste receptors. Methods of identifying compounds that bind to the novel feline bitter taste receptors and modulate their activity are disclosed. | 2014-09-18 |
20140273002 | NANOSPLASMONIC IMAGING TECHNIQUE FOR THE SPATIO-TEMPORAL MAPPING OF SINGLE CELL SECRETIONS IN REAL TIME - A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging. | 2014-09-18 |
20140273003 | SUPER-RESOLUTION FLUORESCENCE LOCALIZATION MICROSCOPY - The present invention relates generally ultrasensitive assays for use in diagnostics and in methods of drug screening and personalizing therapy for an individual patient. Specifically, the present invention relates to improved imaging and computational methods for detecting molecular phenotypes. | 2014-09-18 |
20140273004 | MOLECULES AND METHODS FOR ITERATIVE POLYPEPTIDE ANALYSIS AND PROCESSING - Reagents and methods for the digital analysis of proteins or peptides are provided. Specifically provided herein are proteins for identifying the N-terminal amino acid or N-terminal phosphorylated amino acid of a polypeptide. Also, an enzyme for use in the cleavage step of the Edman degradation reaction and a method for using this enzyme are described. | 2014-09-18 |
20140273005 | Magnecytometer For The Detection Of One Or More Analytes - A system and method for analyzing a sample of liquid having an NMR signal in response to a magnetic field for the presence of an analyte. Included is an NMR device having a testing section that is adapted to contain a liquid and apply a magnetic field to the liquid. A complex comprised of a conjugate having a field gradient bound to the analyte that is of sufficient magnitude to quench the NMR signal of the liquid when in the test section whereby the presence of the complex is determined by the absence of the NMR signal. | 2014-09-18 |
20140273006 | METHOD OF THERAPY SELECTION FOR PATIENTS WITH CANCER - The present invention provides methods for detecting, measuring and quantitating the expression and activation states of signal transduction analytes in cells such as tumor cells. The invention also provides methods for selecting therapy, optimizing therapy, monitoring therapeutic efficacy, and/or detecting therapeutic resistance. Accordingly, the methods are useful for improving cancer therapy selection and disease monitoring. | 2014-09-18 |
20140273007 | DEVICES AND METHODS FOR SCREENING NIPPLE ASPIRATE FOR MARKERS INDICATIVE OF RISK OF DEVELOPING BREAST CANCER - A diagnostic test system, apparatus and method for identifying individuals at risk for developing or having breast cancer by detection of at least one marker associated with increased breast cancer risk in nipple aspirate fluid is provided. A testing device for screening nipple aspirate fluid for the presence of biomarkers associated with breast cancer and methods of identifying individual biomarkers and biomarker panels for evaluating the risk that an individual has developed or will develop breast cancer by assaying nipple aspirate fluid is described. | 2014-09-18 |
20140273008 | METHODS TO ASSESS ENZYME ACTIVITY USING MASS SPECTROMETRIC IMMUNOASSAY - Methods to assess biological enzyme activity directly in a complex clinical sample. To such a sample, e.g., plasma, an exogenous synthetic peptide substrate for an enzyme present in the sample and to be assessed is added. The sample then undergoes mass spectrometric immunoassay (MSIA) to monitor the mass spectral profile of the immuno-purified exogenous synthetic peptide substrate directly from the sample. Using the resulting profile, parameters such as enzyme activity and effect of enzyme modulators, including therapeutics administered to an individual for a condition involving the enzyme, may be determined. | 2014-09-18 |
20140273009 | COMPOSITIONS AND METHODS FOR PERFORMING ASSAYS - The disclosure relates to compositions for use in assays, the compositions comprising at least one latent fluorophore including at least one enzyme-reactive quenching group and a conjugative group; and a support connectable to the latent fluorophore by the conjugative group. The disclosure further relates to methods of measuring the presence and/or concentration of an analyte, as well as methods of measuring the relative activity of at least two enzymes. | 2014-09-18 |
20140273010 | ACID-CLEAVABLE AND CLICKABLE AFFINITY CAPTURE PROBE - The present disclosure relates to biological probes useful for detecting the presence of a target molecule. The biological probes are capable of forming complexes with the target molecule that are stable to reduction, oxidation and hydrolysis. | 2014-09-18 |
20140273011 | DETECTION METHODS OF NADP(H) USING MBFP - Provided is a detection method of NADP(H) from the change of a fluorescence intensity by a reaction between metagenome-derived blue fluorescent protein (mBFP) and NADPH. More particularly, the present invention relates to methods for detecting NADP(H) using mBFP or his-mBFP, or methods for detecting NADP(H) for measuring an activity of NADP(H) dependent dehydrogenase or oxidoreductase. | 2014-09-18 |
20140273012 | DIAGNOSTIC TEST DEVICE WITH IMPROVED STRUCTURE - The present disclosure relates to diagnostic test devices that provide increased comfort and ease of use. The diagnostic test device can include a test member, such as a lateral flow assay test strip. The test device can further comprise a housing that comprises a substantially arch shaped handle. The housing of the test device can comprise a base member that is attached to a curved lower surface of the housing and that can improve stability of the device in an upward facing position as well as enable angled positioning of the device relative to a flat, horizontal surface. The curvature of the test device in particular can provide secure handling of the device while also improving ease and comfort of use thereof. The disclosure further relates to methods of determining the presence of an analyte in a fluid sample and methods for evaluating a test result of a personal use diagnostic test device. | 2014-09-18 |
20140273013 | IMMUNOASSAYS AND METHODS OF DETECTING AND MEASURING INTACT FIBROBLAST GROWTH FACTOR 23, AND C-TERMINAL AND N-TERMINAL FRAGMENTS THEREOF - Immunoassays and methods for detecting and quantifying biological levels of intact fibroblast growth factor (FGF)-23, as well as the N-terminal and C-terminal fragments thereof in a biological sample. The relative amounts or ratios of FGF-23 relative the N-terminal and C-terminal fragments can also be determined. The systems and methods deploy antibodies that are specific to antigenic regions formed upon either the N-terminal or C-terminal regions of FGF-23 and are systematically applied such that intact FGF-23 and the fragments thereof can be detected and quantified. In certain embodiments, dissimilar labels conjugated to tracer antibodies or labeled antibodies specific to N-terminal and/or C-terminal tracer antibodies are utilized to facilitate detection and quantification of both whole length FGF-23 and any fragments thereof. | 2014-09-18 |
20140273014 | ELECTROCHEMICAL METHODS AND DEVICES FOR AMENDING URINE SAMPLES FOR IMMUNOSENSOR DETECTION - The present invention is directed to methods and devices for amending undiluted and partially diluted urine samples in a manner suitable for performing immunoassays for target analytes, for example NGAL. Generally, the urine sample is treated with reagents including at least one of buffer materials, water soluble proteins, urease, and other interferent mitigants. These reagents control the pH of the urine sample in a manner suitable for immuno-binding reactions and ameliorate interferences, particularly during the detection step. | 2014-09-18 |
20140273015 | BINDING COMPOUNDS TO HUMAN Beta 1-ADRENORECEPTOR (Beta 1-AR) AND THEIR USE IN MEASUREMENT OF AUTO-ANTI- Beta 1-AR ANTIBODIES - The present disclosure relates to binding compounds/antibodies that bind to the second extracellular loop of the human β1-adrenoreceptor (β1-AR-ECII) that are produced by/obtainable from a host cell/hybridoma with a deposit number selected from the group consisting of DSM ACC3121, DSM ACC3174, DSM ACC3175, DSM ACC3176 and DSM AC-C3177. The binding compounds/antibodies are particularly useful in determination of auto-anti-β1-AR antibodies in an in vitro cell based assay system in order to characterize and to identify auto-antibodies directed against the β1-AR-ECII in a biological sample. Further aspects of the disclosure are nucleic acid molecules encoding said binding compounds/antibodies, vectors, host cells, methods for producing the binding compounds/antibodies of the disclosure as well as a kit comprising the binding compounds/antibodies of the present disclosure. | 2014-09-18 |
20140273016 | DEVICE AND METHOD FOR THE DETECTION AND DIAGNOSIS OF AGGRESSIVE AND METASTATIC CANCER AND CANCER STEM CELLS EMPLOYING PODOCALYXIN AND TRA BIOMARKERS - The invention relates to devices and methods to detect podocalyxin and TRA molecules in a biological sample obtained from a patient and therefore, to detect the presence of podocalyxin-expressing and TRA-expressing cancer stem cells in a patient. The invention further relates to devices and methods to determine a diagnosis of aggressive or metastatic cancer by employing podocalyxin and TRA as biomarkers. | 2014-09-18 |
20140273017 | PRO-ANGIOGENIC AND ANTI-ANGIOGENIC HEMATOPOIETIC PROGENITOR CELL POPULATIONS - Methods for identifying active angiogenesis and vasculopathy are described. More particularly, the present disclosure relates to cellular biomarkers, and to methods of screening cellular biomarkers for identifying active angiogenesis. | 2014-09-18 |
20140273018 | FLOW-THROUGH CELL COUNTING ASSAY - A method for quantitatively measuring white blood cell (leukocyte) count and/or white blood cell (leukocyte) subsets count involves adding specific antibodies labeled with a marker to the biological fluid sample, capture of white blood cells from a fluid sample by a retainer, removal of other than leukocyte cells and other interfering substances by washing or using specific magnetic beads, and reading the result. The device for use in the present method includes a retainer for leukocyte cells and an absorption pad for taking up all excess washing solution flowing past the sample. | 2014-09-18 |
20140273019 | Methods of Evaluating BAFF - The present disclosure provides compositions and methods relating to the evaluation of BAFF in a biological sample from a subject. | 2014-09-18 |
20140273020 | SYSTEM FOR RAPIDLY DETECTING INFECTIOUS AGENTS USING A HYBRIDOMA-BASED BIOSENSOR - A system for detecting infectious agents in biological samples in real time that includes a sample to be tested for at least one specific infectious agent; and at least one biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; wherein the biosensor emits a detectable signal when it reacts with the specific infectious agent; wherein the biosensor is a hybridoma cell that naturally expresses an endogenous anti-target antigen specific IgM; and wherein the hybridoma cell has been converted to a B cell receptor biosensor by introducing a detectable reporter gene into the cell. | 2014-09-18 |
20140273021 | VITAMIN D ASSAYS - The disclosure relates to methods for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid sample. The disclosure further relates to kits for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid. | 2014-09-18 |
20140273022 | BIOMARKERS FOR DETECTING THE PRESENCE OF BACTERIA - In some aspects, provided are methods relating to the use of bacterial N-methyl-2 superfamily proteins as a biomarker for the presence of bacteria in a sample. The invention also relates to novel methods of diagnosis of the presence of bacteria in a liquid or solid sample, detection of bacterial infections in humans or animals, and use of antibodies or other specific binding molecules capable of binding to N-methyl-2 superfamily proteins. | 2014-09-18 |
20140273023 | GEMCITABINE IMMUNOASSAY - The present invention comprises novel analogs of gemcitabine and novel gemcitabine immunogens teased out of, i.e., derived from, the 5′-hydroxy position of gemcitabine. The invention also comprises unique monoclonal antibodies generated using gemcitabine linked immunogens as well as unique conjugates and tracers which antibodies, conjugates, and tracers are useful in immunoassays for the quantification and monitoring of gemcitabine in biological fluids. | 2014-09-18 |
20140273024 | SYSTEM AND METHOD FOR DETERMINING RISK OF DIABETES BASED ON BIOCHEMICAL MARKER ANALYSIS - A method for predicting risk of gestational diabetes mellitus (GDM) in a pregnant individual includes measuring one or more biochemical markers in a blood sample obtained from the pregnant individual to determine one or more biomarker levels, where the one or more measured biochemical markers includes at least one of PAI-2 and sTNFR1, identifying, for each of the one or more measured biochemical markers, a difference between the measured biomarker level and a corresponding predetermined control level, and, responsive to the identifying, determining a prediction corresponding to a relative risk of the pregnant individual having or developing GDM. | 2014-09-18 |
20140273025 | SYSTEM AND METHOD FOR DETERMINING RISK OF PRE-ECLAMPSIA BASED ON BIOCHEMICAL MARKER ANALYSIS - A method for predicting risk of pre-eclampsia in a pregnant individual includes measuring one or more biochemical markers including an RBP4 biochemical marker in a blood sample obtained from the pregnant individual to determine one or more biomarker levels including an RBP4 biomarker level, identifying, for each of the one or more measured biochemical markers, a difference between the measured biomarker level and a corresponding predetermined control level, and, responsive to the identifying, determining a prediction corresponding to a relative risk of the pregnant individual having or developing pre-eclampsia. | 2014-09-18 |
20140273026 | Methods for Detecting Ehrlichia Infection - The present invention provides an isolated and purified heat shock protein 60 (Hsp60) peptide having the amino acid sequence of SEQ ID NO:2. The instant invention is also directed to a vaccine against | 2014-09-18 |
20140273027 | Serological Methods and Diagnostic Tests for Syphilis Antibodies - A non-treponemal diagnostic test for syphilis infection includes initially dissolving cholesterol in an organic solvent and further diluting the dissolved cholesterol in an ethanol solution comprising cardiolipin and lecithin, permitting a volume of the antigen solution to evaporate in place within a container, rinsing the coated container with buffered saline, stabilizing the antigen coating by overcoating the antigen coating with an inert protein dissolved in buffered saline, decanting the overcoat solution, air-drying the container, and sealing the container in vapor-proof pouches with desiccant, providing an enzyme-labeled conjugate component of a syphilis infection test that is formulated to be compatible with a lipid nature of the cholesterol, the cardiolipin, and the lecithin VDRL antigens, providing a sample diluent that is formulated to be compatible with the lipid nature of the VDRL antigens, and providing a wash fluid that is formulated to be compatible with the lipid nature of the VDRL antigens. | 2014-09-18 |
20140273028 | PREGNANCY TESTING - A method of determining the stage (gestation) of a pregnancy—comprising the steps of quantifying the amount of the hormone hPL or a fragment thereof in a body fluid sample derived from a human female selected from a blood, plasma, serum and/or urine sample, and establishing the stage of pregnancy corresponding thereto. The disclosure also extends to a device adapted to detect the levels/amount of hPL or a fragment thereof in a body fluid sample, derived from a human female, selected from a blood, plasma, serum and/or urine sample, for establishing the stage of pregnancy. | 2014-09-18 |
20140273029 | METHODS AND COMPOSITIONS FOR ENHANCING IMMUNOASSAYS - Embodiments of the methods, compositions, and systems provided herein relate to enzymatic enhancement of immunoassays using photonic sensor arrays. | 2014-09-18 |
20140273030 | HUMAN BIOMARKER TEST FOR MAJOR DEPRESSIVE DISORDER - Materials and methods related to diagnosing depression disorders, using a multi-parameter biomarker system and algorithms related thereto. | 2014-09-18 |
20140273031 | IN-VITRO METHOD FOR MONOCLONAL ANTIBODY PRODUCTION - A method of producing an IgG type monoclonal antibody in vitro is described. The method includes the steps of: (A) providing B-cells obtained from a transgenic non-human mammal whose genome comprises a disruption of an Act1 gene; (B) contacting the B cells with an antigen under conditions which result in formation of an antibody specific to the antigen by the B cells; (C) fusing the B cells with one or more immortal cells, to produce one or more hybridomas which express IgG type monoclonal antibodies that specifically bind to the antigen; (D) selecting a hybridoma which expresses an antibody specific to the antigen; and (E) obtaining monoclonal antibody produced by the selected hybridoma. Kits for carrying out the method of producing an IgG type monoclonal antibody are also described. | 2014-09-18 |
20140273032 | COMPOSITIONS AND METHODS FOR DIAGNOSIS OF SCHIZOPHRENIA - Provided is a method for diagnosis of schizophrenia, which comprises: detecting G72 gene product in a body fluid sample from a subject by an assay to determine G72 expression level; comparing said G72 expression level to a baseline G72 expression; and relating the G72 expression level to the patient's risk of schizophrenia by assigning an increased risk of schizophrenia when said G72 expression level is greater than said baseline G72 expression. Through detecting G72 expression level in a peripheral sample, the method can be simply performed by an in vitro assay and accurately predict or diagnose a subject with schizophrenia. | 2014-09-18 |
20140273033 | BIOMARKERS FOR MULTIPLE SCLEROSIS - Described is the diagnosis of neurological disorders, more specifically, the diagnosis of multiple sclerosis. A biomarker panel is provided that can be used to detect if a subject has multiple sclerosis. Also described are methods of identification of such biomarkers. | 2014-09-18 |
20140273034 | ELECTROCHEMICAL METHODS AND DEVICES FOR AMENDING URINE SAMPLES FOR IMMUNOSENSOR DETECTION - The present invention is directed to methods and devices for amending undiluted and partially diluted urine samples in a manner suitable for performing immunoassays for target analytes, for example NGAL. Generally, the urine sample is treated with reagents including at least one of buffer materials, water soluble proteins, urease, and other interferent mitigants. These reagents control the pH of the urine sample in a manner suitable for immuno-binding reactions and ameliorate interferences, particularly during the detection step. | 2014-09-18 |
20140273035 | ASSAY WITH INCREASED DYNAMIC RANGE - Provided herein are assays and kits useful for avoiding “prozone phenomenon” or “hook effect” and which expand the range of accurately measurable analyte concentrations. | 2014-09-18 |
20140273036 | LUMINESCENT DETECTION OF INORGANIC PHOSPHATE AND COUPLED REACTIONS - Luminescent detection of inorganic phosphate is carried out in an assay through the intermediary enzymatic production of ADP. ADP is converted to ATP which is used in a luminescent reaction. The assay can be used to monitor coupled enzyme reactions which use or generate inorganic phosphate and the modulation of such reactions. | 2014-09-18 |
20140273037 | COMPOSITIONS AND METHODS DIRECTED TO CRISPR/CAS GENOMIC ENGINEERING SYSTEMS - The invention relates to engineered CRISPR/Cas9 systems for genomic modification in mammalian cells. The present specification describes the design and testing of a polynucleotide encoding the | 2014-09-18 |
20140273038 | BIOLUMINESCENT METAL ION ASSAY - A method for determining metal ions, both qualitatively and quantitatively, is disclosed. The method utilizes emission from fluorescence resonance energy transfer from a luciferase-carbonic anhydrase conjugate or fusion protein to an acceptor ligand in the presence of metal ion bound to the protein to measure free metal ion concentrations down to picomolar concentration ranges. The method is relatively insensitive to contaminants, and so can be used to measure metal ion concentrations in cells, body fluids or environmental samples without extensive sample preparation. | 2014-09-18 |
20140273039 | ASSAY FOR CLOSTRIDIUM BOTULINUM NEUROTOXIN - The present invention relates to a method of determining Botulinum toxin (BoNT) based on a luminescence assay. The present application further relates to a peptide that is susceptible to proteolytic cleavage by BoNT which is suitable for that method. | 2014-09-18 |
20140273040 | METHOD FOR PRODUCING RECOMBINANT PROTHROMBIN, VECTOR DNA, AND REAGENT KIT - The present invention provides a method for producing recombinant prothrombin. The method comprises: providing a vector DNA into which a gene encoding a tag and a gene encoding prothrombin are incorporated, wherein the tag is selected from the group consisting of MBP, SUMO, and NusA; and expressing a tag fusion type prothrombin in a lepidopteran insect or cultured cells of the lepidopteran insect. | 2014-09-18 |
20140273041 | BOTTLED GLUCOSE SENSOR WITH NO HANDLING - A device and system for automatic handling of a sensor strip by a part of meter includes a sensor strip having a first section, a second section, and an intermediate section. The sensor strip includes at least a first opening about a first end thereof and a second opening about a second end thereof. A meter part includes a pair of pivoting catches configured to engage and grasp a sensor strip from a container containing a plurality of sensor strips. The sensor strip may thus be removed from a container for testing without need for manual handling of the strip by a user. | 2014-09-18 |
20140273042 | PREDICTIVE CALIBRATION - A portable medical monitor system generates a current calibration curve for generating the estimate of the level of an analyte, such as glucose, being monitored. The current calibration curve is based on at least two measured data values of the level being monitored. The system determines a transformation function based on the calibration curve and at least one preceding calibration curve such that the transformation function produces a predictive calibration curve, and generates an estimated level value of the level being monitored, based on sensor output from a sensor associated with the portable medical monitor system, in accordance with the predictive calibration curve. | 2014-09-18 |
20140273043 | TRI-COLOR DUAL GLUCOSE AND OXYGEN SENSORS AND METHODS OF PREPARING AND USING THEM - The present disclosure relates to an optical fluorescence sensor comprising a probe for sensing glucose, an intra-reference probe, and a matrix. The present disclosure also relates to an optical fluorescence dual sensor comprising a probe for sensing glucose, a probe for sensing oxygen, an intra-reference probe, and a matrix. The present disclosure additionally relates to methods of preparing these sensors and methods of using them. | 2014-09-18 |
20140273044 | METHOD FOR REPLACING BIOMARKERS OF PROTEIN KINETICS FROM TISSUE SAMPLES BY BIOMARKERS OF PROTEIN KINETICS FROM BODY FLUIDS AFTER ISOTOPIC LABELING IN VIVO - Provided herein are method for measuring the rate of synthesis, breakdown, transport, or other kinetic parameters of a protein in a tissue of medical interest, without requiring physical sampling of the tissue, by a measurement of the protein in a body fluid. Methods may include selecting one or more target proteins in a tissue; administering an isotope-labeled molecule to a subject for a period of time sufficient for said isotope-labeled molecule to enter into and label the one or more target proteins to produce one or more isotope-labeled target proteins; collecting a volume of a body fluid, wherein the volume comprises one or more isotope-labeled target proteins that escaped or were released from the tissue; enriching or isolating the one or more isotope-labeled target proteins from the volume; performing a mass spectrometric measurement of the isotopic content, rate of incorporation, and/or pattern or rate of change in isotopic content and/or pattern of isotope labeling of the one or more enriched or isolated isotope-labeled target proteins; and calculating at least one kinetic parameter of the one or more enriched or isolated isotope-labeled target proteins, where the kinetic parameter of the one or more isotope-labeled target proteins from the volume of a body fluid reflects the corresponding kinetic parameter of the one or more target proteins in the tissue; and inferring the at least one kinetic parameter of the one or more target proteins in the tissue based on the corresponding at least one kinetic parameter of the one or more target proteins in the body fluid. | 2014-09-18 |
20140273045 | Modular Biochemical Signaling Laboratory Breadboard for Disease Research, Drug Discovery, Cell Biology, and Other Applications - A “breadboard” approach by which a biochemical signaling process, pathway, or network under study is separated or segmented into interconnected smaller portions, at least one of which can to a degree of approximation be accurately emulated with a replica microscale and/or nanoscale fluidic implementation whose constituent species can be closely controlled and at least one aspect of whose behavior can be measured. Control and measurement information interfaces with a computer that executes algorithms comprising one or more of a control process, control event-script, experiment, data recording, and mathematical model. A model can be used to simulate the actions, behavior, or other aspects of another portion of the biochemical signaling process, pathway, or network. Replica constituents can include enzymes, other proteins, lipids, ions, peptides, and other materials provided under controlled conditions and timing, as well as varying degrees of competitive species, drugs, environmental influences, and substitute or representative molecular crowding. | 2014-09-18 |
20140273046 | METHOD AND DEVICE FOR RAPID DETECTION OF BACTERIAL ANTIBIOTIC RESISTANCE/SUSCEPTIBILITY - Described herein is a method and a device for expediting delivery of an agent to a damaged bacterial cell. In one embodiment, the methods and devices are useful for screening candidate antibiotics. In another embodiment, the methods and devices described herein are used to determine susceptibility of bacteria to an antibiotic. The methods also provide a method for determining an appropriate antibiotic to treat an individual having a bacterial infection. | 2014-09-18 |
20140273047 | Method of detecting Mycobacterium tuberculosis complex by cell filtrate protein 10-loaded detonation nanodiamond - A method of detecting | 2014-09-18 |
20140273048 | SYSTEMS AND METHODS FOR ANALYZING ANIMAL FEED - The present invention relates to systems and methods for analyzing animal feeds. In particular, the present disclosure relates to in vitro systems and methods for analyzing animal feed for metabolism of nutrients and energy sources. | 2014-09-18 |
20140273049 | DIAGNOSTIC MARKER FOR DIGESTIVE ORGAN CANCER AND INSPECTION METHOD FOR DIGESTIVE ORGAN CANCER - A diagnostic marker for digestive organ cancer according to the present invention is used for determining whether or not to have a digestive organ cancer. The diagnostic marker for digestive organ cancer contains at least one of N-binding type sugar chains released from a glycoprotein contained in blood and represented by the following formulas (1) to (6). This makes it possible to provide a diagnostic marker for digestive organ cancer capable of being used for an inspection method of easily determining whether or not to have a digestive organ cancer at an early stage, and an inspection method for digestive organ cancer of easily determining whether or not to have a digestive organ cancer at an early stage. | 2014-09-18 |
20140273050 | METHODS OF MEASURING CELL VIABILITY IN TISSUE ENGINEERED PRODUCTS - This invention provides methods of measuring the viability of cultured cells by detecting one or more cell death-stable proteins or enzyme activities. Methods provided by the invention correlate viability to relative levels of enzyme activity in cell-containing and non-cell-containing fractions of a cell culture. | 2014-09-18 |
20140273051 | CHEMICAL SENSING APPARATUS HAVING MULTIPLE IMMOBILIZED REAGENTS - An apparatus sensing two or more reactants or analytes in a sample is provided. The apparatus has an one or more light sources emitting energy with two or more detection targets having an immobilized reagent within the target surface. One or more detectors are provided where the two or more detection targets having immobilized reagent thereon are in communication with the sample and the immobilized reagent interacts with the sample. Energy is incident on the targets from the at least one light source such that the energy is changed by the interaction and the change is in turn detected by the at least one detector and associated with a measurement of the level of the reactant or analyte in the sample. A method of making a sensing apparatus and a method of sensing using the sensing apparatus are also disclosed. | 2014-09-18 |
20140273052 | CHEMICAL SENSING APPARATUS HAVING MULTIPLE IMMOBILIZED REAGENTS - A sensor system in a water treatment system has a housing, controller, one or more light sources, one or more sensors and one or more targets having an immobilized reagent thereon. Light source emits light energy into the housing that is incident upon the target with the immobilized reagent and the reagent being in contact with water from the system. The immobilized reagent interacts with a reactant in the water such that the interaction changes the state of the reagent. When energy from the light source is incident on the target with the immobilized reagent the energy shows a change detectable by the sensor such that the changed energy is detectable by and collected at the sensor and data on the energy is communicated to the controller. The data is then correlated as a representation of a desired variable to be measured for the water in the water treatment system. | 2014-09-18 |
20140273053 | CELL CHIPS - Disclosed is a cell chip and methods for the use thereof, wherein the cell chip includes a substrate made of an opaque material and having a plurality of insertion holes formed therein, a filler made of a transparent material and inserted into each of the insertion holes so as to protrude from the substrate, and a biomatrix which is formed on the filler and immobilizes a biomaterial. Also, another substrate having a plurality of wells which store a fluid is further provided thus forming a 3D cell chip. | 2014-09-18 |
20140273054 | COUPLED ENZYME-BASED METHOD FOR ELECTRONIC MONITORING OF BIOLOGICAL INDICATOR - A sterilization indicator system and method of using the system to determine efficacy of a sterilization process. The system includes a vial having a first compartment containing spores of one or more species of microorganism; a second compartment containing a growth medium with a disaccharide, an oligosaccharide or a polysaccharide in which the vial is free of monosaccharide; an enzyme, capable of acting upon the monosaccharide to yield reaction products and electron transfer, disposed on two or more electrodes adapted to carry an electrical signal resulting from the electron transfer, the pair of electrodes positioned to contact the combined contents of the first compartment and the second compartment during incubation; and an apparatus linked or linkable to the electrodes and adapted to detect and measure the electrical signal resulting from electron transfer when the enzyme acts upon the monosaccharide. | 2014-09-18 |
20140273055 | NONINVASIVE METHOD FOR MEASURING OXIDATIVE STRESS AND OXIDATIVE DAMAGE FROM SKIN: OXIDATIVE STRESS AND OXIDATIVE DAMAGE BIOMARKERS - A noninvasive method for diagnosing skin health in a subject comprising collecting a skin sample/epithelial cell sample from the subject; detecting a level of one or more biomarkers selected from the group consisting of Myeloperoxidase and oxidized lipids in the epithelial cell sample/skin cell sample; diagnosing the subject as having oxidative stress and/or oxidative damage based on the level of a detected biomarker. Further, a noninvasive method for evaluating the efficacy of products for skin health. | 2014-09-18 |
20140273056 | Device And Method For Extracting A Targeted Fraction From A Sample - A device and a method for isolating a target from a sample are provided. The target is bound to solid phase substrate to form a target bound solid phase substrate. The device includes a first plate having a first region for receiving at least a portion of the sample. A second plate is spaced from the first plate by a distance and has a first region for receiving a reagent. A force attracts the target bound solid phase substrate toward the first region of the second plate such that the target bound solid phase substrate in the portion of the sample are drawn through the air gap and into the reagent by the force. | 2014-09-18 |
20140273057 | METHODS OF CELL CULTURE - Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using DMSO, are described. | 2014-09-18 |
20140273058 | Integrated Membrane for Preservation of Biomolecules - An apparatus and method for moiety specific sample extraction from a complex sample matrix in a flow through process is provided. The present invention relates to an integrated membrane for separation and preservation of biomolecules. Classes of biomolecules, for example, those in blood, can be separated and preserved. For blood separation, the apparatus includes a housing having a matrix portion, and a fluid collection portion disposed within an inner cavity thereof. The housing also includes an aperture permitting access to the inner cavity. The apparatus also includes a matrix disposed within the matrix portion. The matrix includes a first layer for collecting substantially all cells from the fluid, a second layer for protein adsorption, and a third layer for nucleic acid adsorption. Fluid enters the housing through the aperture, passes through the matrix, and into the fluid collection portion. The method includes passing fluid through a matrix; collecting substantially all cells from the fluid in a layer of the matrix; adsorbing protein from the fluid in a layer of the matrix; and adsorbing nucleic acid from the fluid in a layer of the matrix. | 2014-09-18 |
20140273060 | Reagents, Systems and Methods for Analyzing White Blood Cells - Aspects of the invention include WBC analysis reagents, systems and methods that can be used for analyzing a sample of whole blood to identify, classify, and/or quantify white blood cells (WBC) and WBC sub-populations in the sample. The WBC analysis reagents of the present disclosure generally include at least one membrane-permeable fluorescent dye, a WBC protecting reagent, and a surfactant. In some embodiments, the WBC reagents include a suitable amount of an osmolality adjusting component to adjust the osmolality of the WBC reagent into a desired range. | 2014-09-18 |
20140273061 | Reagents, Systems and Methods for Analyzing Erythrocytes and Platelets - Aspects of the invention include hematology analysis reagents, systems and methods that can be used to preserve blood cell morphology and integrity as well as provide sample integrity and optical clarity to facilitate optical analysis of blood samples. In some embodiments, the reagents include a non-phosphate organic buffer and a sphering surfactant. The pH and osmolality of the reagents may be adjusted to desired ranges. In addition, the reagents can be simply diluted with de-ionized water prior to use. | 2014-09-18 |
20140273062 | Cell Pack for the Growth and Manipulation of Three Dimensional Cell Cultures - Described herein is a sealed cell pack with a permeable membrane for growth and manipulation of three-dimensional cell cultures. This allows a cell culture to be removed from the laboratory and subjected to real world insults before being returned to culture conditions for continued growth and study. One application is for use in the study of the direct effects of blast waves on neuronal cells and methods for mitigating this response. | 2014-09-18 |
20140273063 | TISSUE ENGINEERED MODEL - A tissue engineered model (TEM) structure, an apparatus and method for making a TEM structure, and methods of using a TEM structure are disclosed. In an embodiment, the TEM structure includes at least one TEM segment. Each TEM segment includes a frame defining a bounded area, the frame having a height, a first edge, and a second edge opposite the first edge, each of the first edge and the second edge defining a perimeter of the bounded area, and the height defining a distance between the first edge and the second edge; a membrane affixed to the first edge about a perimeter of the frame; and a solidified gel and cell matrix disposed within the bounded area within the frame, wherein the solidified gel and cell matrix substantially fills a volume defined by the bounded area and the height of the frame. | 2014-09-18 |
20140273064 | Portable Blood Count Monitor - This disclosure describes the development of a sample preparation, measurement, and analysis method that permits accurate characterization of red blood cells, platelets, and white blood cells, including a 3-part differential of the white blood cells count, to be performed on small volumes of a biological sample. This method is compatible with compact and portable instrumentation that permits the sample collection to be performed in a subject's home and analysis to be performed elsewhere by transmission of the data to a laboratory or doctor's office. | 2014-09-18 |
20140273065 | NONINVASIVE METHOD FOR MEASURING ANTIMICROBIAL PEPTIDES FROM SKIN AS AN OBJECTIVE MEASUREMENT OF NATURAL PROTECTION FROM MICROBES - A noninvasive method for measuring skin health in a subject comprising collecting an epithelial cell/skin cell sample from the subject; detecting a level of one or more antimicrobial peptide biomarkers in the epithelial cell sample/skin cell sample; diagnosing the subject as having a defense mechanism due to microbial attack on skin or a measure of the system being in balance with environment homeostasis based on the level of one or more antimicrobial peptide biomarkers selected from the group consisting of Histone H2B type 1-M, Histone H2A, Histone H4, Protein S100-A7, Protein S100-A8, Protein S100-A9, Cathepsin G, Neutrophil defensin 3 (Defensin, alpha 3) (HNP-3), Dermcidin, Ribonuclease 7 (RNase 7), human beta-defensin -2 (nBD-2) and Beta-defensin 103 (hBD-3) and listed in Table 1 wherein the AMP families include bactericidal permeability-increasing protein (BPI), Defensin, Histone, Pore-forming toxin, S100 protein, Cytotoxin, Serine Protease 51, Dual Oxidase, transcription regulation, and mixtures thereof. Further, a noninvasive method for evaluating the efficacy of products for skin health. | 2014-09-18 |
20140273066 | AUTOMATED CELL COLLECTION AND SMEARING - The present invention provides systems, devices, and methods for cell collection and/or cell smearing (e.g., on a slide). In particular, provided herein is an cell collection device, a translating slide holder, systems comprising such devices, and methods of use thereof. | 2014-09-18 |
20140273067 | FLOWCELL SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES - The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. In some aspects the system comprises an analyzer which may be a visual analyzer. In one aspect, this disclosure relates to a particle imaging system comprising a flowcell through which a sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis of samples. Other compositions, methods and features of this disclosure are disclosed herein. | 2014-09-18 |
20140273068 | AUTOFOCUS SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES - Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. A contrast pattern for autofocusing is provided on the flowcell, for example at an edge of a rear illumination opening. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Blood cell images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data. | 2014-09-18 |
20140273069 | PREDICTING HUMAN DEVELOPMENTAL TOXICITY OF PHARMACEUTICALS USING HUMAN STEM-LIKE CELLS AND METABOLOMICS - The invention provides biomarker profiles of metabolites and methods for screening chemical compounds including pharmaceutical agents, lead and candidate drug compounds and other chemicals using human stem-like cells (hSLCs) or lineage-specific cells produced therefrom. The inventive methods are useful for testing toxicity, particularly developmental toxicity and detecting teratogenic effects of such chemical compounds. Specifically, a more predictive developmental toxicity model, based on an in vitro method that utilizes both hSLCs and metabolomics to discover biomarkers of developmental toxicity is disclosed. | 2014-09-18 |
20140273070 | ASPIRATION-FREE WELL PLATE APPARATUS AND METHODS - A well plate includes a including a top portion, a bottom portion and a membrane disposed between the top portion and the bottom portion. The top portion defines a sample well in fluid communication with an opening defined by the membrane and in fluid communication with a reservoir defined by the bottom portion. The well plate is configured to be used in a centrifugation process of a test sample including a sample material and a wash liquid. The test sample configured to be received within the sample well and the reservoir. The membrane configured to filter the wash liquid from the test sample during the centrifugation process such that the wash liquid can pass from the reservoir, through the membrane and can be captured within a collection chamber while the sample material remains within the reservoir. | 2014-09-18 |
20140273071 | AUTOMATED SPECIMEN TRANSFER SYSTEM AND METHOD - An automated sample processing system includes a sample input adapted to receive at least one sample container holder manually provided by a user, a consumable input adapted to receive one or more unused consumable supplies manually provided by a user, a waste output adapted to receive used consumable supplies, an automated processing apparatus, and a sample output, wherein the automated processing apparatus includes a decapper adapted to remove a lid from the at least one sample container, at least one agitator adapted to agitate and thereby homogenize the contents of the at least one sample container, a sample collector adapted to remove fluid specimen from the at least one sample container and transfer the specimen to at least one output carrier, and a capper adapted to replace the lid on the at least one sample container. | 2014-09-18 |
20140273072 | STERILIZATION INDICATOR INCLUDING A SIMPLIFIED GENETICALLY ENGINEERED BIOLOGICAL INDICATOR - A sterilization indicator, including a first compartment containing a genetically engineered biological indicator, and a second compartment containing an enzyme substrate, the second compartment adapted to maintain the enzyme substrate separate from the biological indicator during sterilization, and to permit the enzyme substrate to contact the biological indicator after the biological indicator has been exposed to the sterilization medium; in which the genetically engineered biological indicator comprises at least one test organism and at least one reporter gene suitable for producing an indicator enzyme, the reporter gene being taken up by the test organism; and the test organism is free of any active or activatable repressor gene that would inhibit expression of the reporter gene if present in the test organism or sterilization indicator, and the indicator enzyme and the enzyme substrate are selected such that enzymatic action of the indicator enzyme upon the enzyme substrate yields a detectable signal. | 2014-09-18 |
20140273073 | STERILIZATION INDICATOR OF OXIDATIVE STERILANTS - A sterilization indicator for oxidative sterilants, comprising a first compartment comprising spores of one or more microorganism species, wherein the spores have been pretreated with and comprises a compound comprising a transition metal ion that is reactive with an oxidative sterilant; a second compartment comprising a growth medium and adapted to combine contents of the first compartment with contents of the second compartment for incubation after the sterilization indicator has been exposed to an oxidative sterilant; and an agent disposed in the growth medium and selected to indicate viability of the spores after the sterilization indicator has been exposed to the oxidative sterilant. | 2014-09-18 |
20140273074 | YEAST SCREENS FOR TREATMENT OF HUMAN DISEASE - Screening methods for identifying substances that provide therapeutic value for various diseases associated with protein misfolding are provided. Genetic and chemical screening methods are provided using a yeast system. The methods of the invention provide a rapid and cost-effective method to screen for compounds that prevent protein misfolding and/or protein fibril formation and/or protein aggregation which includes numerous neurodegenerative diseases including Parkinson's disease, Alzheimer's disease, Huntington's disease as well as non-neuronal diseases such as type 2 diabetes. | 2014-09-18 |
20140273075 | METHODS, SYSTEMS AND DEVICES FOR DETERMINING WHITE BLOOD CELL COUNTS FOR RADIATION EXPOSURE - Systems and methods are provided for imaging at least one eye with optical components and processing the image of the at least one eye to determine radiation exposure. An image of one or more eyes may be received. The systems and methods may distinguish blood cell types in the image; quantify circulating levels of a selected blood cell type; output a number of blood cells per volume of the selected blood cell type; and determine a radiation dose based on the number of blood cells per volume of the selected blood cell type. | 2014-09-18 |
20140273076 | DYNAMIC RANGE EXTENSION SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES - For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category. | 2014-09-18 |
20140273077 | DEVICE FOR THE ASEPTIC EXPANSION OF CELLS - A closed system suitable for the aseptic culturing therapeutic cells comprises a vessel comprising: a gas permeable portion suitable for supporting cell growth and allowing delivery of gases to the cells during culturing, a vent comprising a conduit having an exterior orifice and an interior orifice spaced apart therefrom, the vent extending from the exterior of the system into the internal volume of the vessel and terminating therein with the interior orifice, wherein the interior orifice is arranged such that during filling and emptying of liquid medium it is not susceptible to blockage by liquid, the exterior orifice is adapted to connect to an aseptic filter thereby allowing passage of gases through the filter into the vessel or out of the vessel, as required to achieve the entry and exit of fluids and cells into the vessel, a port or ports adapted to allow introduction of fluids and cells aseptically into the vessel, and a port or ports adapted to allow fluids to exit the system without exposing the system to the external environment and adapted such that cells grown therein may exit the system under gravity when the system is orientated to put the cells in fluid communication with the exit port and the latter is opened. The present system facilitates the aseptic manufacturing of cells for use in therapy, without the need for a clean-room environment because no open processing steps are required. | 2014-09-18 |
20140273078 | APPARATUS FOR WORKING ON HISTOLOGICAL SAMPLES - The invention relates to an apparatus for treatment of histological samples. The apparatus includes at least a working station, in which the sample gets into contact with a liquid reagent. Further, the apparatus includes a purification device, which blows off at least a part of the reagent adhering to the sample and/or the sample holding arrangement by means of a gas flow, or which sucks off at least a part of the reagent adhering to the sample and/or a sample holding arrangement. | 2014-09-18 |
20140273079 | BIOLOGICAL SPECIMEN HANDLING APPARATUS AND METHOD - In accordance with one aspect of the invention, a mold is provided for receiving a tissue specimen and molten embedding material that improves the ease of withdrawing the embedded tissue specimen. The mold has a barrier coating intimately bonded to one or more surfaces of the mold that repels the attraction of the embedding material to the one or more mold surfaces and produces a positive meniscus of the embedding material in the mold. The barrier coating permits the mold to be re-used without needing to manually re-apply a release agent to the mold before using the mold. Further, the barrier coating permits the use of more aggressive draft angles than conventional molds by reducing the frictional engagement between the embedding material and the mold cavity surfaces. | 2014-09-18 |
20140273080 | METHOD FOR METABOLOMIC SAMPLE PREPARATION BASED ON IONIC LIQUID DISPERSIVE LIQUID-LIQUID MICROEXTRACTION - Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided. | 2014-09-18 |
20140273081 | METHOD AND COMPOSITION FOR STAINING AND SAMPLE PROCESSING - The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents, one or more permeabilizing agents, and one or more fixing agents. The particle contrast agent composition can include Crystal Violet, New Methylene Blue, Saponin, and Gluteraldehyde. | 2014-09-18 |
20140273082 | METHOD AND COMPOSITION FOR STAINING AND PROCESSING A URINE SAMPLE - The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300. | 2014-09-18 |
20140273083 | METHOD FOR PROCESSING AND EMBEDDING TISSUE - A method for treating a tissue sample including placing at least one tissue sample on an cassette which has: a retaining member, a base and at least one biasing element; placing the at least one tissue sample in the tissue cassette; attaching the base and the retaining member to retain the tissue sample; processing the tissue sample in the tissue cassette with one or more solvents; and embedding the tissue sample in a paraffin to form an portion of paraffin in which the tissue sample is embedded in the tissue cassette, wherein the embedding comprises adding molten paraffin to the interior area of the tissue cassette and allowing the paraffin to become solid. | 2014-09-18 |
20140273084 | TISSUE CASSETTE WITH RETRACTABLE MEMBER - An apparatus for holding a tissue sample having a retaining member with a first tissue engaging surface and at least one biasing element. The first tissue engaging surface is moveably attached to the retaining member. The apparatus also has a base comprising a second tissue engaging surface which is configured to engage the retaining member to form an interior area with the first and second tissue engaging surfaces facing each other. The apparatus also has a retracting member connected to the retaining member which is configured to retract the first tissue engaging surface and compress the biasing element to form a gap between the tissue sample and one of the first tissue engaging surface and the second tissue engaging surface. | 2014-09-18 |