27th week of 2012 patent applcation highlights part 45 |
Patent application number | Title | Published |
20120171674 | Detection of Cells Expressing T1R2 Taste Receptor - Binding assays for identifying compounds that modulate human T1R2 polypeptide associated taste are disclosed. These assays detect the specific binding of compounds to a human T1R2 polypeptide or the modulation of the specific binding of a compound that specifically binds to a human T1R2 polypeptide. The binding assays may include the use of detectable labels, e.g., radionuclides, enzymes, fluorophases, and the like. Compounds identified in these binding assays have putative application as T1R2 taste modulators, particularly sweet taste, and potentially are useful additives in compositions for human or animal consumption. | 2012-07-05 |
20120171675 | METHOD FOR ISOLATING NUCLEIC ACIDS - The invention describes a method of and kits for isolating and/or purifying nucleic acids, more specifically short-chain nucleic acids such as miRNA, from a nucleic acid-containing starting material, characterized by the following method steps of:
| 2012-07-05 |
20120171676 | HIGHLY SENSITIVE RAPID ISOTHERMAL METHOD FOR THE DETECTION OF POINT MUTATIONS AND SNPs, A SET OF PRIMERS AND A KIT THEREFOR - The present invention refers to a method for detecting a point mutations of a nucleotide sequence by an improve- ment of the LAMP (loop amplification mediated polymerization) amplification method, as well as to a set of primers and kit there- for. As a non limitative embodiment, the invention refers to the G1849T mutation of the JAK2 gene. | 2012-07-05 |
20120171677 | COMPENSATION FOR SPECTRAL CROSSTALK IN MULTIPLEX NUCLEIC ACID AMPLIFICATION - A method includes performing a nucleic acid amplification of a nucleic acid sample using a detection probe, wherein the nucleic acid amplification occurs over one or more interrogation periods, and, from the nucleic acid amplification, acquiring amplification data that indicates an amount of nucleic acid present for each of the one or more interrogation periods. The method also includes, based on the amplification data, determining a crosstalk correction value associated with a spectral neighbor to the probe to reduce spectral crosstalk from the spectral neighbor; and applying the crosstalk correction value to amplification data collected from multiplex nucleic acid amplifications of nucleic acid samples. | 2012-07-05 |
20120171678 | APPARATUS FOR THERMAL CYCLING - This invention provides a system for performing PCR, and real time PCR in particular, with great speed and specificity. The system employs a heat block containing a liquid composition to rapidly transfer heat to and from reaction vessels. The system makes use of the reflective properties of the liquid metal to reflect signal from the PCR into the vessel and out the top. In this way, the signal can be measured by an optical assembly in real time without removing the vessels from the heat block. | 2012-07-05 |
20120171679 | Method for Rapid Detection and Identification of Bioagents in Epidemiological and Forensic Investigations - The present invention provides methods for rapid forensic investigations by identification of bioagents associated with biowarfare and acts of terrorism or crime. The methods are also useful for epidemiological investigations by genotyping of bioagents. | 2012-07-05 |
20120171680 | SINGLE-MOLECULE PCR FOR AMPLIFICATION FROM A SINGLE NUCLEOTIDE STRAND - A method, apparatus and system for performing single molecule PCR for amplification from single stranded polynucleotides. | 2012-07-05 |
20120171681 | OLIGONUCLEOTIDES, METHODS AND KITS FOR DETECTING AND IDENTIFYING VANCOMYCIN-RESISTANT ENTEROCOCCUS - The present invention relates to methods for detecting the presence or absence of vancomycin resistant enterococci (VRE) in a sample using a multiplex polymerase chain reaction (mtx-PCR) assay. Furthermore, the present invention relates to oligonucleotide primers and kits for the detection of VRE. | 2012-07-05 |
20120171682 | METHOD FOR DETECTING, IDENTIFYING AND/OR QUANTIFYING CARBON NANOTUBES - The present invention relates to a method and a kit for detecting, optionally identifying and optionally quantifying at least one carbon nanotube possibly included in a sample, including the steps consisting in: (a) subjecting said sample to conditions enabling the amplification of a nucleotide sequence using primers capable of amplifying said nucleotide sequence, the possibly included carbon nanotube having been functionalized by said nucleotide sequence prior to step (a), and (b) detecting, optionally identifying and optionally quantifying the amplification product possibly obtained after step (a). | 2012-07-05 |
20120171683 | ANALYSIS OF FRAGMENTED GENOMIC DNA IN DROPLETS - Method of analyzing genomic DNA. Genomic DNA including a target may be obtained. The genomic DNA may be fragmented volitionally to produce fragmented DNA. The fragmented DNA may be passed through a droplet generator to generate aqueous droplets containing the fragmented DNA. An assay may be performed on the droplets to determine a level of the target. In some embodiments, the droplets may contain the genomic DNA at a concentration of at least about five nanograms per microliter, the droplets may be generated at a droplet generation frequency of at least about 50 droplets per second, the droplets may have an average volume of less than about 10 nanoliters per droplet, the droplets may generated at a flow rate of greater than about 50 nanoliters per second, or any combination thereof. | 2012-07-05 |
20120171684 | DDAO COMPOUNDS AS FLUORESCENT REFERENCE STANDARDS - According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I): | 2012-07-05 |
20120171685 | BUFFERS FOR THE STABLE STORAGE OF NUCLEIC ACIDS - Provided herein are buffers for the stabilization of nucleic acid molecules. The buffers find particular use for the stabilization of trace amounts of nucleic acid molecules in a variety of environments, including repeated freeze/thaw cycles. For example, in some embodiments, provided herein are compositions comprising tris(hydroxymethyl)aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), polyadenylic acid, and a synthetic DNA oligonucleotide. | 2012-07-05 |
20120171686 | METHODS FOR THE IDENTIFICATION OF MICRORNA AND THEIR APPLICATIONS IN RESEARCH AND HUMAN HEALTH - The press invention concerns a method for prediction and identification of microRNA precursors (pre-microRNA) and microRNA molecules using data processing programs and databases. The invention also pertains to the isolated form of these pre-microRNAs, microRNA molecules and derived nucleic acids there of. The invention also relates to recombinant vector, host cell, support, pharmaceutical composition or kit comprising such microRNA molecules or there of derivated molecules. The invention also applies to the use of such microRNA molecules and/or their identified targets in research, prognostic, diagnostic tools/methods as well as for therapeutic applications. | 2012-07-05 |
20120171687 | Response Prediction in Cancer Treatment - Methods comprising detecting whether the p53 gene is present in native form on DNA molecules in tumor cells or cell-free tumor DNA in a sample of body fluid or a tissue sample of the tumor patient or whether the p53 gene on said DNA molecules in said tumor cells or cell-free tumor DNA has one or more mutations. In some specific cases, these methods involve determining the p53 status of the tumor patient. Kits and compositions for the practice of such methods are also disclosed. | 2012-07-05 |
20120171688 | Gene Expression Markers for Colorectal Cancer Prognosis - A method of predicting clinical outcome in a subject diagnosed with colorectal cancer comprising determining evidence of the expression of one or more predictive RNA transcripts or their expression products in a biological sample of cancer cells obtained from the subject. | 2012-07-05 |
20120171689 | FUIDI HERD MANAGEMENT SCHEMA - The invention is a herd management schema based upon the inventor's analysis of the natural history of bovine infection due to | 2012-07-05 |
20120171690 | Transgenic Animals for Analyzing CYP3A4 Cytochrome P450 Gene Regulation - The invention relates to the generation of non-human transgenic animals comprising a reporter construct for producing a detectable amount of a reporter molecule operably linked to a transcriptional regulatory nucleic acid molecule from the human CYP3A4 gene located between the initiation of transcription site of the gene and a position located 13,000 nucleotides upstream from the site. The invention also relates to the use of these animals for determining the effect of a compound, particularly, but not exclusively, a xenobiotic or steroid, on the regulation of expression of the CYP3A4 gene in a human. | 2012-07-05 |
20120171691 | METHOD FOR MONITORING THE BISULFITE-MEDIATED CONVERSION OF DNA - The present invention relates to a method for monitoring the progression of the bisulfite-mediated conversion of DNA during DNA methylation analysis. The method is based on the reaction of the enzyme uracil-DNA-glycosylase (UNG) with at least one labeled DNA reporter molecule, the reporter molecule comprising at least one unmethylated cytosine residue in its sequence. After bisulfite-mediated conversion of unmethylated cytosine residues in uridin residues UNG removes the uracil bases from the DNA backbone, thus making it susceptible to heat-induced hydrolytic cleavage. Finally, the labels released from the DNA reporter molecule during this fragmentation process are detected. | 2012-07-05 |
20120171692 | Composition For Use In Identification Of Bacteria - The present invention provides oligonucleotide primers and compositions and kits containing the same for rapid identification of bacteria by amplification of a segment of bacterial nucleic acid followed by molecular mass analysis. | 2012-07-05 |
20120171693 | Methods for Generating Novel Stabilized Proteins - The disclosure provides methods for identifying and producing stabilized chimeric proteins. | 2012-07-05 |
20120171694 | PREDICTIVE MARKERS AND BIOMARKER PANELS FOR OVARIAN CANCER - Methods are provided for predicting the presence, subtype and stage of ovarian cancer, as well as for assessing the therapeutic efficacy of a cancer treatment and determining whether a subject potentially is developing cancer. Associated test kits, computer and analytical systems as well as software and diagnostic models are also provided. | 2012-07-05 |
20120171695 | SPONTANEOUSLY CONTRACTING FISH CELL AGGREGATES, USE THEREOF AND METHOD FOR THE PRODUCTION THEREOF - The invention relates to an in vitro method for producing spontaneously contracting fish cell aggregates, to the fish cell aggregates obtained thereby, and to the use thereof, in particular for testing biological active substances and pharmaceuticals. The in vitro method according to the invention for producing contracting fish cell aggregates comprises the following steps: a) mechanical comminution and/or partial enzymatic digestion of fish embryos or fish larvae; b) transfer of the comminuted and/or partially digested fish tissue into an enzyme-free medium and removing the supernatant by centrifuging to obtain a cell pellet; c) re-suspension of the cell pellet in a cell culture medium; d) culturing the cells, with the medium being changed at least once, until spontaneously contracting cell aggregates form. | 2012-07-05 |
20120171696 | Gene Defects And Mutant ALK Kinase In Human Solid Tumors - In accordance with the invention, novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have now been identified in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule-Associated Protein-Like 4 (EML-4) and TRK-Fusion Gene (TFG). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of this new fusion protein enables methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides. | 2012-07-05 |
20120171697 | Method of Determining Inhibitors of Coagulation - A homogeneous method of determining inhibitors of proteolytically active coagulation factors (anticoagulants) in a sample, in particular direct thrombin and factor Xa inhibitors, and also a test kit to be used in such a method. Use is made of ligands which bind to the proteolytically active coagulation factor but are not cleaved by the latter and compete with the anticoagulant to be determined. | 2012-07-05 |
20120171698 | METHOD AND DEVICE FOR RAPID PARALLEL MICROFLUIDIC MOLECULAR AFFINITY ASSAYS - Disclosed are methods and devices for rapid parallel molecular affinity assays performed in a microfluidic environment. The invention exploits hydrodynamic addressing to provide simultaneous performance of multiple assays in parallel using a minimal sample volume flowing through a single channel. | 2012-07-05 |
20120171699 | ANTIBODIES FOR THE DETECTION OF INTEGRIN COMPLEXES IN FFPE MATERIAL - The invention relates to antibodies that are capable to bind the extracellular domain of integrin. Another object of the invention concerns the use of said antibodies for detecting integrins in archival formalin fixed paraffin embedded (FFPE) tissue. The invention also relates to methods for preparing monoclonal rabbit antibodies, wherein the immunogen is an insect expression culture-derived recombinant extracellular integrin domain, and another method for screening anti-integrin antibodies that discriminate between closest integrin homologues and that are especially suited for immunohistochemistry in FFPE material. | 2012-07-05 |
20120171700 | CELL IDENTIFYING APPARATUS AND CELL IDENTIFYING METHOD - A cell identifying apparatus includes a capillary through which a sample flow passes, an irradiating unit that irradiates irradiation light, a light receiving unit that receives transmitted light and outputs a transmitted light signal based on the transmitted light, a measuring unit that receives the transmitted light signal output from the light receiving unit and measures the change of the transmitted light signal caused by cells, an identifying unit that identifies whether the cells are peripheral blood cells on the basis of a value of an extreme point in the change of the transmitted light signal, the change being caused by the cells and measured by the measuring unit, a sorting unit that sorts the cells in a predetermined vessel, on the basis of the identification result of the identifying unit, and a supplying unit that supplies the sample flow to the capillary. | 2012-07-05 |
20120171701 | IDENTIFICATION OF REGULATORY T CELLS VIA THE GLOBAL GENE REGULATOR SATB1 - A method for the identification of regulatory T cells based on the diminished abundance or even absence of the global gene regulator SATB1 in such regulatory T cells. In particular, the invention relates to a method utilizing ligands that specifically bind to SATB1 for identifying regulatory T cells which are cells showing a reduced binding to said ligand. Such method is suitable for quality determination of a regulatory T cell population. A kit or diagnostic composition for such method is also disclosed. | 2012-07-05 |
20120171702 | QUANTITATIVE AND SELF-CALIBRATING CHEMICAL ANALYSIS USING PAPER-BASED MICROFLUIDIC SYSTEMS - A method of determining the concentration of a test fluid sample using a paper-based microfluidic system having a plurality of hydrophilic testing zones, including: a) depositing said test fluid sample on at least one said testing zone; b) depositing a plurality of standard fluid samples or reactives of differing known concentrations on other said testing zones; c) introducing an indicator solution to each said test zone to thereby react with the deposited fluid sample and result in a colour intensity change which is a function of the fluid sample concentration; and d) comparing the differences in colour intensity between the test fluid sample and the standard fluid samples or reactives to thereby determine the concentration of said test fluid sample. | 2012-07-05 |
20120171703 | IMIDAZO[1,2-ALPHA]PYRAZIN-3(7H)-ONE DERIVATIVES BEARING A NEW ELECTRON-RICH STRUCTURE - The present invention relates to compound of formula I: and their use as chemiluminescent and/or bioluminescent reagents. | 2012-07-05 |
20120171704 | Elisa for a naturally-occurring soluble truncated form of IL-23 receptor - A naturally-occurring soluble truncated IL-23Rα protein (i.e., Δ9 IL-23Rα) is shown to be present in a biological sample and can serve as a diagnostic tool for autoimmune diseases. There is provided an enzyme-linked immunosorbent assay (ELISA) and test kit for the serological detection of the soluble truncated form of IL-23Rα protein. More particularly, antibody-sandwich ELISA method and kits for Δ9 IL-23Rα as an antigen were developed to detect Δ9 IL-23Rα levels in biological samples from a mammal and a human patient and are used as a diagnostic index. The present disclosed ELISA has utility as a diagnostic tool to detect Crohn's disease in patients using EDTA-plasma. | 2012-07-05 |
20120171705 | COMBINATION OF SPLA2 ACTIVITY AND OXPL/APOB CARDIOVASCULAR RISK FACTORS FOR THE DIAGNOSIS/PROGNOSIS OF A CARDIOVASCULAR DISEASE/EVENT - The use of a combination of sPLA2 activity and OxPL/apoB cardiovascular risk factors for the diagnosis/prognosis of a cardiovascular disease/event or for the monitoring of a cardiovascular disease. | 2012-07-05 |
20120171706 | GLUCOSE MEASUREMENT USING A CURRENT SOURCE - A blood glucose meter comprises a blood sample test strip, a constant current source, a precision timer, a digital processor and memory, and an analog measurement circuit, e.g., voltage comparator, analog-to-digital converter (ADC), etc., that eliminates the complex analog front end and other related circuits of present technology glucose meters. When a blood sample is applied to the blood sample test strip a charge, Q, develops from the reaction between an enzyme in the test strip and the blood sample. The constant current source injects a constant current value, I, into the charge, Q, on the blood sample test strip over a precisely measured time determined by when the excess charge, Q, has been removed from the test strip. The amount of charge, Q, is determined by Q=I*T, the charge, Q, is then converted into a blood glucose level for display. | 2012-07-05 |
20120171707 | ASSAY FOR INHIBITORS OF SUMO AND SUMO-TARGETED UBIQUITIN LIGASE PATHWAY - The present invention provides a method for determining a putative agent that inhibits the SUMO or the SUMO-targeted ubiquitin ligase pathway comprising the steps of contacting mutant MOT1 cells with the putative agent under conditions (1) permitting mutant MOT1 cell growth in the presence of a SUMO or SUMO-targeted ubiquitin ligase pathway inhibitor and (2) not permitting mutant MOT1 cell growth in the absence of a SUMO or SUMO-targeted ubiquitin ligase pathway inhibitor, and measuring cell growth. | 2012-07-05 |
20120171708 | MODIFIED FLAVIN ADENINE DINUCLEOTIDE-DEPENDENT GLUCOSE DEHYDROGENASE - Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent. | 2012-07-05 |
20120171709 | Method for Identifying Bacteria from the Bacillus Cereus Group - The present invention relates to the field of microbiological testing of food. It relates to a method for identifying bacteria of the Bacillus cereus group, comprising the steps consisting in: | 2012-07-05 |
20120171710 | Method for Grass Species Identification - The present invention relates to the use of at least one peptide comprising or consisting of a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5, for determining the presence of extracts from at least one grass species in a composition. | 2012-07-05 |
20120171711 | NOVEL METHOD FOR ISOLATING TRICHINELLA OR OTHER PARASITES FROM ORGANIC TISSUE - The present invention relates to a method for the detection of essentially intact encapsulated or non-encapsulated parasites in meat, comprising the maceration of the meat with an alkaline digestion solution which contains a digestive enzyme that is active in an alkaline environment. Further, uses of a serine endopeptidase in a method for the detection of essentially intact encapsulated or non-encapsulated parasites in meat are described. The present invention also describes serine endopeptidases for use in a diagnostic method for the detection of essentially intact encapsulated or non-encapsulated parasites in meat. Finally, a kit is disclosed which comprises the enzymes and alkaline digestion solutions of the present invention. | 2012-07-05 |
20120171712 | Process for Realising a Device for Collecting and Transferring Samples for Molecular Biology - A process for realising a device ( | 2012-07-05 |
20120171713 | SINGLE CHIP HAVING THE CHEMICAL SENSOR AND ELECTRONICS ON THE SAME DIE - A semiconductor die includes a chemical sensor, a digital to analog converter, and microcontroller formed therein. The chemical sensor detects the presence of a chemical and outputs an analog signal to the digital to analog converter. The analog to digital converter converts the analog signal to a digital signal. The analog to digital converter outputs the digital signal to the microcontroller. Microcontroller calculates a value of the concentration of the selected chemical. | 2012-07-05 |
20120171714 | METHOD AND PARTICLE ANALYZER FOR MEASURING A LOW CONCENTRATION PARTICLE SAMPLE - A method and particle analyzer for measuring a low concentration particles sample disclosed. | 2012-07-05 |
20120171715 | SEMICONDUCTOR BIOSENSORS - The present application relates to semiconductor devices, in particular to a device for monitoring a cell signal such as an electrical signal produced by living cells in response to external stimulation, optionally in real time, comprising (a) at least one discrete area comprising a high electron mobility transistor (HEMT); and (b) non-excitable cells attached to said HEMT (HEMT element) for example, fibroblasts, HEK, CHO cell lines, keratinocytes, etc. Preferably, the HEMT is an AlGaN/GaN FET. Accordingly, the device can be applied in uses and methods for monitoring a cell signal such as an electrical signal produced by living cells in response to external stimulation, optionally in real time. Likewise, the device can be applied for screening compounds that reverse, protect from and/or shield cells from external stimuli which cause damage to cells. Also, kits comprising the device are disclosed. | 2012-07-05 |
20120171716 | Measurement of Mitochondrial Membrane Potential to Assess Organ Dysfunction - The present invention relates to the field of organ transplantation. More specifically, the present invention provides methods for predicting organ function after transplantation. In certain embodiments, the method comprises measuring mitochondrial membrane potential from a biopsy sample from the donor organ. The present invention is also applicable to organ dysfunction in general. More specifically, the methods of the present invention may be useful in formulating prognoses for patients with acute or chronic organ dysfunction due to ischemia, infection, drug injury or age. In this rapid procedure, only small samples of tissue are required, enabling the clinical application of mitochondrial function previously thought impractical. | 2012-07-05 |
20120171717 | METHOD OF SELECTING SAFE PLURIPOTENT STEM CELLS - Provided is a method of selecting highly safe pluripotent stem cells that do not exhibit differentiation resistance, comprising the steps of (1) inducing a pluripotent stem cell to differentiate, (2) culturing the cell under conditions for maintaining undifferentiated state, (3) detecting the generation of an undifferentiated cell by the cultivation, and comparing the finding with a control, and (4) selecting a pluripotent stem cell whose detected value is not more than a control generation value. | 2012-07-05 |
20120171718 | Submerged Perfusion Bioreactor - A device for biological purposes such as cell culturing, enzymatic reactions or filtering of fluid has a body with first and second surfaces. The body is delimited by a rim and an aperture in the center of the body. The aperture is covered at the first and second surface by first and second plates. The first and/or second plate has an inlet orifice allowing liquid medium into the aperture. Rotating means are arranged in the aperture between the first and second plate. At least one recessed portion is a cavity in the rim of the body having a first outlet orifice allowing the liquid medium to flow out of the body. At least one outlet channel connects the circular aperture with the recessed portion. Liquid is pumped into the aperture of the device and pumped through at least one outlet channel. | 2012-07-05 |
20120171719 | USE OF SACCHAROMYCES CEREVISIAE SUC2 GENE IN YARROWIA LIPOLYTICA FOR SUCROSE UTILIZATION - Disclosed herein are transformed | 2012-07-05 |
20120171720 | Method of Making Ribosomes - Methods for making in vitro assembled ribosomal subunits and in vitro assembled ribosomes are provided. Methods of transcribing synthetic rRNA and including the synthetic RNA in a synthetic ribosome are provided. Single vessel methods of transcribing synthetic rRNA, forming a synthetic ribosome that includes the synthetic RNA, and allowing the synthetic ribosome to translate a protein are also provided. Methods of screening for novel, synthetic ribosomal subunits and/or ribosomes are provided. Synthetic replicons and methods of making synthetic replicons are also provided. | 2012-07-05 |
20120171721 | Host Cell Protein Knock-Out Cells for Production of Therapeutic Proteins - The present invention relates to methods and means for making Vitamin K-dependent protein compositions which are devoid or substantially devoid of protein contaminants. In particular, methods and means useful for the reduction or elimination of protein contaminants also being Vitamin K-dependent proteins are described. | 2012-07-05 |
20120171722 | PERSONALIZED PRODUCTION OF BIOLOGICS AND METHOD FOR REPROGRAMMING SOMATIC CELLS - The invention provides a method for producing polypeptide protein products and nucleic acid products having reduced levels of antigenicity in an animal being treated with a biologic product. Somatic cells are isolated from an animal, transformed into pluripotent stem cells, transfected with a nucleic acid(s) of interest, and re-differentiated towards somatic cells known to be high level producers of the desired nucleic acid product. The invention can be used to derive a general cell line to treat populations, racial specific cell lines to treat ethnic groups, or patient specific cell lines to treat individuals. Additionally, the invention provides a method to allow induced pluripotent stem cells to be re-differentiated towards their somatic cell of origin so that the cells can be used to therapeutically treat an animal without resulting teratoma formation. | 2012-07-05 |
20120171723 | FGF HOMOLOGS COMPOSITIONS AND USES THEREOF - The present invention relates to zFGF5 compositions and methods of using the compositions to proliferate chondrocytes and their progenitors, and to induce deposition of cartilage. zFGF5 compositions are disclosed for treating disorders associated with chondrocytes, such as cartilage injuries and defects. In addition, methods for treating neurological disorders, such as stroke, are disclosed, and methods for using zFGF5 compositions to stimulate growth of cells associated with neurological injury and disease are disclosed. | 2012-07-05 |
20120171724 | High yield suspension cell line, system, and method for making same - A system and method of adapting host cells to suspension cell culture and a suspension cell line produced thereby are disclosed. The method includes the serial replating of substantially undiluted culture cells onto a surface area until cell clumps are visualized and then, upon cell clumping, moving the cells into a suspension culture system. | 2012-07-05 |
20120171725 | Amplicon Rescue Multiplex Polymerase Chain Reaction for Amplification of Multiple Targets - Disclosed is a method for amplifying and detecting polynucleotides which can provide sensitive, specific detection of multiple targets from a clinical specimen within a relatively short time. | 2012-07-05 |
20120171726 | RAPID WHOLE GENOME AMPLIFICATION - The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes. | 2012-07-05 |
20120171727 | NOVEL GENOME WALKING METHOD FOR CLONING OF UNKNOWN DNA SEQUENCES ADJACENT TO KNOWN SEQUENCE - A method and a kit for cloning a nucleotide sequence adjacent to a known nucleotide sequence by PCR are disclosed. The method includes 1) preparing a DNA fragment with cohesive ends by cleaving a DNA that contains the known first nucleotide sequence and the second nucleotide sequence adjacent thereto using a restriction enzyme; 2) modifying the 3′ end of the DNA fragment with a nucleotide analogue to block further elongation of the DNA fragment; 3) linking the cohesive ends of the 3′ end-modified DNA fragment with a linker, adapter or cassette having a cohesive end sequence complementary thereto; and 4) performing PCR using a known first nucleotide sequence-specific primer, and a linker, adapter or cassette sequence-specific primer. | 2012-07-05 |
20120171728 | PROCESS FOR AMPLIFYING DNA USING TETRATETHYLENE GLYCOL, KIT OF PARTS THEREFOR AND USE THEREOF - Disclosed is a process for amplifying DNA, a process for preparing and amplifying DNA, a kit of parts for DNA amplification and DNA preparation, and the use thereof, all of which are characterized by the use of tetraethylene glycol. Moreover, the use of tetraethylene glycol in a reaction solution by carrying out a DNA amplification and a reaction solution containing tetraethylene glycol for carrying out a DNA amplification are disclosed. | 2012-07-05 |
20120171729 | METHODS FOR THE SYNTHESIS OF POLYADENYLIC ACID - The present invention provides compositions, kits, and methods for synthesizing polyadenylic acid using polynucleotide phosphorylase, adenosine diphosphate, a buffering agent, and a divalent metal cation. In certain embodiments, the adenosine diphosphate is present at a concentration between about 5.0 mM and about 100 mM, and the buffering agent has a pH around 8.0. In some embodiments, the prepared polyadenylic acid compositions are free of detectable nucleic acid. | 2012-07-05 |
20120171730 | METHOD AND SUBSTANCES FOR PREPARATION OF N-SUBSTITUTED PYRIDINIUM COMPOUNDS - Methods for the synthesis of N-substituted pyridinium compounds by using an N-heteroaryl substituted pyridinium salt (Zincke salt) and reacting it with a nucleophilic amine are provided. Novel purine-substituted pyridyl compounds, which may be useful reagents in the above reaction, are also disclosed. | 2012-07-05 |
20120171731 | ALPHA-AMYLASE BLEND FOR STARCH PROCESSING AND METHOD OF USE THEREOF - The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a | 2012-07-05 |
20120171732 | Non-pressurised pre-treatment, enzymatic hydrolysis and fermentation of waste fractions - The present invention relates to a process for production of fermentation products, including bioethanol by non-pressurised pre-treatment, enzymatic hydrolysis and fermentation of waste fractions containing mono- and/or polysaccharides, having a relatively high dry matter content. The process in its entirety, i.e. from non-pressurised pre-treatment over enzymatic hydrolysis and fermentation to sorting of fermentable and non-fermentable solids can be processed at a relatively high dry matter content in a single vessel or similar device using free fall mixing for the mechanical processing of the waste fraction. | 2012-07-05 |
20120171733 | MICROALGAE FERMENTATION USING CONTROLLED ILLUMINATION - Bioreactors and methods for cultivating microalgae are provided herein. The bioreactor and methods include features and modifications to improve heterotrophic growth efficiency by providing a light signal. | 2012-07-05 |
20120171734 | EXTRACTION OF EXTRACELLULAR TERPENOIDS FROM MICROALGAE COLONIES - The invention provides methods of extracting and quantifying extracellular terpenoid hydrocarbons, e.g., botryococcenes, methylated squalenes, and carotenoids, from terpenoid-producing and secreting green microalgae. | 2012-07-05 |
20120171735 | PRODUCTION OF TRIACYLGLYCERIDES, FATTY ACIDS, AND THEIR DERIVATIVES - The invention relates to production of triacylglycerols in cells. | 2012-07-05 |
20120171736 | POWDERY LIPASE PREPARATION AND USE THEREOF - The present invention discloses a powdery lipase preparation in which the particles constituting the powdery lipase have 3,000 to 40,000 pores/mm | 2012-07-05 |
20120171737 | PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS - Mutants of various polyhydroxyalkanoate (PHA) synthases capable of synthesizing a lactate polymer (PLA) and a lactate copolymer (PLA copolymer), and a method of preparing a lactate polymer and a lactate copolymer using the same are provided. More specifically, a mutant of polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8, and a method of preparing lactate polymer and lactate copolymer using the mutant of synthase are provided. The polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8 can have an activity of synthesizing a lactate polymer and a lactate copolymer by an amino acid sequence mutation affecting an activity of synthesizing a lactate polymer, and can produce a lactate polymer and a copolymer that have different features, respectively, by using the mutants of the synthase. | 2012-07-05 |
20120171738 | ENZYMATICALLY CATALYZED METHOD OF PREPARING MONO-ACYLATED POLYOLS - The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacyl-glycerol lipase mutants, as for example derived from | 2012-07-05 |
20120171739 | Xylitol Producing Microorganism Introduced with Arabinose Metabolic Pathway and Production Method of Xylitol Using the Same - The present invention relates to an efficient production method of xylitol by using the xylitol producing microorganism introduced with arabinose metabolic pathway to inhibit the production of arabitol, the byproduct, and instead to use arabinose only for cell metabolism in xylose/arabinose mixed medium. More precisely, to express efficiently L-arabinose isomerase (araA), L-ribulokinase (araB) and L-ribulose-5-phosphate 4-epimerase (araD) in | 2012-07-05 |
20120171740 | DE-FATTED SOY PRODUCTION PROCESS AND VALUE ADDED BY-PRODUCTS FROM DE-FATTED SOY FLOUR - An improved process for producing de-fatted soy utilizing a de-fatted soy flour and for producing value added by-products from de-fatted soy flour wherein soybeans are de-hulled and the de-hulled stream ground to a flour consistency. The ground soy flour is mixed with water and other additives to produce a vitamin and mineral enriched stream that is then filtered to various value added by-products. In a preferred embodiment the vitamin and mineral enriched stream is filtered through a 0.1-1.0 micron membrane to produce a de-fatted soy product stream and a fatted soy product stream. The fatted soy product stream can be dried to produce dry, less than 12% water by weight, product B for use in cosmetics and pharmaceutical products. The de-fatted soy product can be filtered through reverse osmosis (RO) filtration unit to obtain a vitamin and mineral enriched product stream that can be dried to powder form and used as a food supplement additive I. If desired the full fatted soy flour or the de-fatted soy product stream can be combined with whole stillage from an ethanol process and used in producing various other value added products. | 2012-07-05 |
20120171741 | Renewable Xylenes Produced from Bological C4 and C5 Molecules - The present invention is directed to a method for preparing renewable and relatively high purity p-xylene from biomass, and from C | 2012-07-05 |
20120171742 | Method and Apparatus for Treating Fermentable Substances - The present invention relates to a method for treating fermentable organic substances, the method comprising the anaerobic fermentation thereof in a fermentation tank ( | 2012-07-05 |
20120171743 | THERMOTOLERANT ACTINOMADURA STRAIN CAPABLE OF DEGRADING POLYESTERS - A new thermotolerant | 2012-07-05 |
20120171744 | MATERIALS FOR MAGNETIZING CELLS AND MAGNETIC MANIPULATION - A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field. | 2012-07-05 |
20120171745 | Method for Selectively Damaging and Killing Tumor Cells and Apparatus Therefor - A method and an apparatus capable of selectively damaging and killing tumor cells includes a step of irradiating tumor cells with a UV pulse flash having continuous emission spectra ranging at least from 230 to 270 nm, outside a living body of a human or a living body of a non-human animal or in a living body of a non-human animal. The UV pulse flash may have an accumulated irradiation amount per unit area that is achieved at a distance of 8 cm from a light source having an integrated output of, for example, 90, 180-7100 or 14200 J. | 2012-07-05 |
20120171746 | METHOD AND SYSTEM FOR MANIPULATION OF CELLS - The invention is directed to a method for the manipulation of at least one cell, the method comprising the steps of depositing a metal onto the surface of a substrate, placing the at least one cell at or near the surface of the substrate, and irradiating the surface of the substrate with at least one laser pulse. The inventive method is characterized by the formation of surface structures with a size of one micrometer or less on the surface of the substrate prior to depositing the metal thereon. The invention is also directed to a system for the manipulation of at least one cell, the system comprising a substrate with surface structures having a size of 1 micrometer or less, wherein a metal is deposited on the surface structures, and wherein the system further comprises a laser for irradiating the surface structures. | 2012-07-05 |
20120171747 | SIGNAL AMPLIFICATION MICROSPHERES, THEIR USE IN ONE-STEP AND MULTI-STEP ANALYTICAL AMPLIFICATION PROCEDURES AND METHODS FOR THEIR PRODUCTION - The present invention relates to microspheres comprising protein signal precursor molecules, or a carrier protein bonded to signal precursor molecules, wherein said signal precursor molecules are activatable to generate a detectable signal whilst remaining bonded to the carrier protein. Also disclosed is a method of making such microspheres comprising the steps of mixing protein molecules with a matrix former in solution; adding a reducing reagent to the mixture; removing the reducing reagent; and removing the matrix former to leave microspheres of protein molecules. Also disclosed are bioassay methods using the microspheres to provide signal amplification, including an amplification cycling procedure. | 2012-07-05 |
20120171748 | Halomonas Strain WDG195-Related Alpha-Amylases, And Methods Of Use, Thereof - Compositions and methods relating to an alpha-amylase enzyme obtained from | 2012-07-05 |
20120171749 | Methods for monitoring multiple gene expression - The present invention relates to methods for monitoring differential expression of a plurality of genes in a first | 2012-07-05 |
20120171750 | PURIFICATION OF VACCINIA VIRUSES USING HYDROPHOBIC INTERACTION CHROMATOGRAPHY - The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production. | 2012-07-05 |
20120171751 | NOVEL MICROBE, LIPID MODIFYING AGENT, PROCESS FOR PRODUCING 2-ACYL-LYSOPHOSPHOLIPID, PROCESS FOR PRODUCING DIACYLGLYCEROL, PROCESS FOR PRODUCING CERAMIDE, AND METHOD OF DEGUMMING OIL OR FAT - A new supply source of enzymes useful for modification of phospholipids for example, and a method for producing 2-acyl lysophospholipid, a method for producing monoacylglycerol, and a method for producing ceramide, as well as a new method for degumming fat and oil. A novel microorganism is provided, which belongs to | 2012-07-05 |
20120171752 | Water/Carbonate Stripping For CO2 Capture Adsorber Regeneration And CO2 Delivery To Photoautotrophs - The invention provides systems and methods for the delivery of carbon to photoautotrophs. The invention utilizes low energy regeneration of adsorbent for CO | 2012-07-05 |
20120171753 | SYSTEM AND METHOD FOR TREATING MATERIALS IN POOLS - System and method are provided for treating materials in a liquid in a pool having a bottom, using at least one of a stirring device, a brushing device and a wiping device, and an automatic moving arrangement configured for moving any of these devices in the pool. The stirring device comprises at least one wing having a longitudinal axis and at least two bottom contacting elements spaced from each other along the longitudinal axis of the wing so that, when the wing is in such close proximity to the bottom of the pool that the contacting elements contact its bottom, the contact of the wing with the bottom is prevented by the contacting elements. | 2012-07-05 |
20120171754 | STABILIZED ENZYMATIC COMPOSITION - The invention relates to compositions comprising enzymes and enzyme stabilizing agents. This is achieved by using an enzyme-stabilizing component which comprises a calcium compound with a bidentate ligand and a phenylboronic acid derivative. Enzyme-containing compositions comprising such an enzyme-stabilizing component are advantageously stable in storage. | 2012-07-05 |
20120171755 | FABRICATION OF HOLLOW NANONEEDLES - The present invention provides a method of fabricating a device having hollow nanoneedles which extend through a supporting membrane. The device can be used as a molecular delivery system. | 2012-07-05 |
20120171756 | SYSTEMS AND DEVICES FOR ISOTHERMAL BIOCHEMICAL REACTIONS AND/OR ANALYSIS - An isothermal reaction and analysis system may include a receiver to receive sample holders, a thermal control subsystem to control a temperature of the receiver, an excitation subsystem, a detection subsystem and an analysis subsystem. Excitation sources and/or detectors are positioned to enhance data collection. Sample holders may include filters, selectively blocking and passing wavelengths or bands of electromagnetic radiation. | 2012-07-05 |
20120171757 | CARTRIDGE AND SENSOR-DISPENSING INSTRUMENT - A disposable cartridge adapted to be used with a sensor-dispensing instrument comprises a housing, test sensors, a mechanical mechanism and moveable seals. The housing forms at least one opening therethrough. The test sensors are stacked in the housing. The test sensors are adapted to assist in testing at least one analyte. The mechanical mechanism is adapted to urge the test sensors in a first direction. One of the test sensors is positioned for ejection from the cartridge. The moveable seals is adapted to be in a closed position that seals the at least one opening so as to provide a substantially moisture-proof and a substantially air-tight cartridge, and one of the moveable seals is adapted to be in an open position that allows one of the test sensors to be moved therethrough. | 2012-07-05 |
20120171758 | Device for extracting nucleic acid from a sample - The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample. | 2012-07-05 |
20120171759 | INTEGRATED APPARATUS FOR PERFORMING NUCLEIC ACID EXTRACTION AND DIAGNOSTIC TESTING ON MULTIPLE BIOLOGICAL SAMPLES - The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides. | 2012-07-05 |
20120171760 | NON-INVASIVE SENSING OF BIOPROCESS PARAMETERS - A system and method for measuring at least one bioprocess parameter utilizes a barrier that separates an external sensor from a culture medium. The barrier allows analytes to diffuse in and out of the culture vessel, thereby allowing the bioprocess parameter to be measured non-invasively by the external sensor. | 2012-07-05 |
20120171761 | INDUSTRIALIZED ALGAE CULTURING METHOD AND SYSTEM THEREOF - An industrialized algae culturing method is provided, which comprises the following steps of: placing algae and a culture solution into a pipeline photosynthesis unit for photosynthesis; introducing the photosynthesized algae and the culture solution into a gas intake/venting unit by means of a powered liquid transport unit to remove oxygen from and replenish carbon dioxide into the culture solution; and introducing the photosynthesized algae and the culture solution into the pipeline photosynthesis unit for recycling therein the gas intake/venting unit has a first sealing member and a second sealing member, and the first sealing member and the second sealing member keep the gas intake/venting unit sealed off the external environment during a process of gas intake or venting, thereby keeping the culture solution clean and improving the quality of the algae. Furthermore, the present invention also provides an industrialized algae culturing system. | 2012-07-05 |
20120171762 | Stem and progenitor cell compositions recovered from bone marrow or cord blood; system and method for preparation thereof - The invention includes compositions of stem and progenitor cells recovered from bone marrow or cord blood containing most of the viable CD34+ cells and substantially depleted of red blood cells resident in the original sample, without any xenobiotic additives to aid cell separation. The invention also includes a system and method for preparing the compositions. The system includes a bag set and a processing device, which utilizes an optical sensor, microcontroller, servo motor, accelerometer, load cell, and battery. The system and method utilize centrifugation to stratify the cells into layers and then separate and transfer the stem cells into a stem cell bag. The processing device's microcontroller receives input from the device's accelerometer, load cell and optical sensor to direct the metering valve in the bag set to open and close to permit the transfer of as many stems cells as possible with as few red cells as possible. | 2012-07-05 |
20120171763 | DISSOCIATION METHOD AND DISSOCIATION AGENT FOR AVIDIN AND BIOTIN DERIVATIVES - A method for dissociating avidin or streptavidin efficiently from a biotin derivative, thereby making it possible to isolate a target material efficiently under a mild condition in a short period, and a dissociation agent for use thereof, are provided. | 2012-07-05 |
20120171764 | METHOD OF CELL CULTURES AND DEVICE FOR IMPLEMENTING IT - The invention generally provides a cell culture vessel having at least one first zone and at least one second zone, wherein the first zone is a transfer zone for a culture medium which essentially contains no cells and the second zone is a cell culture zone. The invention further includes methods utilizing the cell culture vessel. | 2012-07-05 |
20120171765 | Factor VII or VIIa - Like Molecules - Conjugates of Factor VII (FVII) and Factor VIIa (FVIIA) are provided, as are methods for preparing them. Methods for producing novel polypeptides contributing to the production of such conjugates are provided. Methods of treatment by administering a FVII or FVIIa conjugate are provided. | 2012-07-05 |
20120171766 | MEDIUM COMPOSITION COMPRISING NEUROPEPTIDE Y FOR THE GENERATION, MAINTENANCE, PROLONGED UNDIFFERENTIATED GROWTH OF PLURIPOTENT STEM CELLS AND METHOD OF CULTURING PLURIPOTENT STEM CELL USING THE SAME - The present invention relates to a medium composition comprising neuropeptide Y, effective for proliferation and maintenance of undifferentiated pluripotent stem cells, and a method for culturing undifferentiated pluripotent stem cells using the same. The present invention improves the culture conditions for undifferentiated pluripotent stem cells, and ultimately, the present invention can be effectively used for the development of large-scale culture systems, thereby acquiring clinically applicable pluripotent stem cells. Further, the present invention relates to a dedifferentiation medium composition comprising neuropeptide Y (NPY), and a method for inducing dedifferentiation (or reprogramming) using the same. The present invention improves the culture conditions for dedifferentiation and contributes to develop technology of producing clinically applicable induced pluripotent stem cells, thereby being used for the development of stem cell therapy. | 2012-07-05 |
20120171767 | COMPOSITIONS AND METHODS FOR GROWING HUMAN EMBRYONIC CELLS - Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing secreted products obtained from the culture medium of human embryonic gem (EG) cell derivatives, such as embryoid body-derived cells. Substrates include compounds such as collagen I, fibronectin, or superfibronectin, or extracellular matrix, typically human derived. | 2012-07-05 |
20120171768 | COLLAGEN-BASED MATRICES FOR VESSEL DENSITY AND SIZE REGULATION - Collagen based-matrices and methods of their use are described. More particularly, collagen-based matrices and methods for regulating vessel density and size within collagen-based matrices are described. | 2012-07-05 |
20120171769 | CELL ATTACHMENT COATINGS AND METHODS - Cell attachment coatings for articles such as implantable medical devices and cell culture vessels are disclosed. The coatings include an intermediate coater layer which includes a sulfonated component that is bonded in the coating by reacted aryl ketone functional groups. The coating also include a second coated layer including an immobilized ECM protein or peptide that includes an active portion of an ECM protein that is able to serve as an outer layer to contact cells during use. The coatings promoted enhanced cell binding and growth. | 2012-07-05 |
20120171770 | BIOENGINEERED SILK PROTEIN-BASED NUCLEIC ACID DELIVERY SYSTEMS - Nucleic acid transfer is achieved using a silk-based delivery system which releases nucleic acids from silk-based complexes. The silk-based complexes, which are composed, for example, of plasmid DNA (pDNA) and recombinant silk containing polycation and specific polypeptides sequences, can show high biocompatibility, high delivery efficiency, cell selectivity and controlled release of nucleic acid for nucleic acid transfection. | 2012-07-05 |
20120171771 | MODIFIED IPS CELLS HAVING A MUTANT FORM OF A HUMAN IMMUNODEFICIENCY VIRUS (HIV) CELLULAR ENTRY GENE - Methods and composition for generation of genetically modified induced pluripotent stem cells and hematopoietic cell derived therefrom are provided. For example, in certain aspects those cells comprise a modified gene structure related to HIV cellular entry, such as CCR5 mutants. | 2012-07-05 |
20120171772 | TAGGED JOINT COMPOUND AND METHOD OF IDENTIFICATION - At least one of these or other problems is reduced using a building material that is uniquely identifiable. A method of making an identifiable gypsum-based building product includes selecting a tagging material occurring naturally in a component of the building material and choosing a carrier substance having a high concentration of the tagging material. By selecting an amount of the carrier substance and adding it to the building material, a building product having a unique product characteristic is created. In a second embodiment of the invention, a second tagging material is combined with the first tagging material to create a unique product. In yet another embodiment of the invention, a supplemental amount of the tagging material is added to the gypsum-based composition in addition to the carrier substance. Some tagging materials are useful in a gypsum-based composition to produce a visual confirmation of the presence of the tagging material. | 2012-07-05 |
20120171773 | Temperature Control Of Enrichment And Separation Columns In Chromatography - A method of analyzing samples includes loading a sufficient quantity of the sample onto a trap column to overload the trap column; heating an analytical column and the trap column to a greater temperature than the analytical column; and pumping a solvent, to the trap column, having a solvent composition profile that, in cooperation with a temperature differential, causes at least some of the components to elute sequentially from the trap column to the analytical column and focus on the analytical column prior to eluting from the analytical column; or optionally: loading a small-molecule sample onto a cooled portion of an analytical column; heating the analytical column; and pumping a solvent, to the heated analytical column, to elute the components from the analytical column. Chromatographic separation includes: a trap column; a separation column; a trap-column heater; a separation-column heater; a solvent pump unit; and a control unit can be used. | 2012-07-05 |