12th week of 2013 patent applcation highlights part 43 |
Patent application number | Title | Published |
20130071831 | METHOD, SYSTEM, AND COMPUTER READABLE STORAGE FOR PODCASTING AND VIDEO TRAINING IN AN INFORMATION SEARCH SYSTEM - A method and system for enhancing search skills of human guides in an information search system. The method allows human guides to be trained when they are not particularly busy fielding search queries from information seekers. The method can include determining whether a guide is idle and presenting to a human guide at least one video or audio source for training the human guide. The video training can include a demonstration of the most effective method for providing search results for an information search query, thereby enabling a guide to learn effective strategies and skills for conducting information searching. The training can contribute to the likelihood of the guide being promoted to a level of a more experienced human guide, thereby allowing greater effectiveness in fielding search queries for information seekers. | 2013-03-21 |
20130071832 | Methods and Systems for Demonstrating Differences Between and Benefits of Products - Disclosed are methods and systems of demonstrating advantages and benefits of premium animal care products, the methods comprising the steps of: presenting a first demonstration aid wherein the first demonstration aid demonstrates a first feature or characteristic; presenting a second demonstration aid wherein the second demonstration aid demonstrates a second feature or characteristic; describing to an audience the first feature or characteristic; describing to an audience the second feature or characteristic; and comparing the first and second features or characteristics to demonstrate the advantages and benefits of the animal care product. The methods and systems can include methods and systems of demonstrating reduced hair ingestion and hairball related effects; methods and systems of demonstrating improvements in skin and coat; and methods and systems of demonstrating better absorption and/or retention of food and nutrients, and production of less waste. | 2013-03-21 |
20130071833 | MEANS AND METHODS FOR DISTINGUISHING FECV AND FIPV - The invention provides methods and means for distinguishing FECV and FIPV, and methods and means for determining whether FIPV is present in a sample. Further provided are primers and probes for detecting FIPV specific nucleic acid sequences encoding a spike protein, antibodies for detecting a FIPV, and an immunogenic composition and use thereof for eliciting an immune response against a feline coronavirus, preferably a FIPV. | 2013-03-21 |
20130071834 | COMPOSITIONS AND METHODS USEFUL FOR HCV INFECTION - The present invention provides compositions comprising cells that can effectively produce HCV after HCV infection, compositions for culturing the cells, methods for making the composition and methods for infecting the cells in the composition with HCV. The present invention also provides methods for assaying HCV production and methods for evaluating compounds that affect the production of HCV. | 2013-03-21 |
20130071835 | Qualitative and Quantitative Analytical Method for Analyzing the Activity Type of an Enzyme that is Activated by Proteolysis - The present invention relates to a qualitative and quantitative analytical method for analyzing the activity type of an enzyme that is activated by proteolysis. The method of the present invention is advantageous in that the activity type of an enzyme present in a specimen is analyzed using an enzyme activity inhibitor and a binder to enable the analysis to be performed in a quicker and more accurate manner as compared to enzyme-activity analysis methods using a simple binder (for example, an antibody). The method of the present invention can enable the analysis of the activity type of an enzyme present in a specimen in a simple manner using less equipment than enzyme-activity analysis methods using a simple binder. The method of the present invention involves simultaneously using the inhibitor and the binder in analyzing the activity type of a target enzyme or an enzyme to enable quick and specific analysis, and high-throughput drug screening can be performed at a high speed due to the above-described characteristics of the method of the present invention. In addition, the method of the present invention can be applied to point-of-care testing (POCT) for the clinical monitoring of the effects and dosage of a drug in a drug administration target (for example, an animal or human). | 2013-03-21 |
20130071836 | COLON CANCER BIOMARKER DISCOVERY - The present application discloses an epigenetic marker for colon cancer. | 2013-03-21 |
20130071837 | Method and System for Characterizing or Identifying Molecules and Molecular Mixtures - A system and method for identifying a material passing through a nanopore filter wherein an electrical signal is detected as a result of the passage and that signal is processed in real-time using mathematical and statistical tools to identify the molecule. A carrier molecule is preferably attached to one or more molecule(s) under consideration using a non-covalent bond and the pore in the nanopore filter is sized so that the molecule rattles around in the pore before being discharged without passing through the filter pore. The present invention includes not only a method and system for identifying the molecule(s) under consideration but also a kit for setting up the filter as well as mathematical tools for analyzing the signals from the sensing circuitry for the molecule(s) under consideration. | 2013-03-21 |
20130071838 | PEPTIDE NUCLEIC ACID PROBES FOR ANALYSIS OF CERTAIN STAPHYLOCOCCUS SPECIES - The present invention relates to peptide nucleic acid (PNA) probes, PNA probe sets and methods for the analysis of certain | 2013-03-21 |
20130071839 | SYSTEMS AND METHODS FOR DETECTING BIOMARKERS OF INTEREST - In some embodiments, a strand displacement system is provided. Such a system may include a first nucleic acid catalyst molecule; a nucleic acid gate molecule, wherein the first nucleic acid catalyst molecule binds the nucleic acid gate molecule forming a nucleic acid gate-catalyst complex and releases an output molecule; and a nucleic acid sink molecule. The nucleic acid sink molecule sequesters a putative second nucleic acid catalyst, wherein the second nucleic acid catalyst differs from the first nucleic acid catalyst molecule by at least one nucleotide. In some aspects, the first nucleic acid catalyst may include a biomarker of interest or a nucleic acid aptamer which binds an amino acid-based biomarker of interest. | 2013-03-21 |
20130071840 | ENHANCED DETECTION OF NON-SOMATIC CIRCULATING NUCLEIC ACIDS - Methods, equipment and software for the detection of abnormal nucleic acid or fetal nucleic acid sequences circulating in the subject's blood or other body fluids. A scan of a subject's circulating nucleic acid (CNA) is compared to the CNA Data On Normal Healthy Population or to a scan of the subject's own Somatic Nucleic Acid (SNA). Abnormal peaks are identified by subtracting out the “normal” or somatic peaks. The resultant anomalous peaks are compared to anomalous CNA data to identify the abnormalities, or the anomalous peaks of the subject's CNA are isolated, amplified, and sequenced or probed to determine the nature of the anomaly. | 2013-03-21 |
20130071841 | TRANSLOCATION AND MUTANT ROS KINASE IN HUMAN NON-SMALL CELL LUNG CARCINOMA - In accordance with the invention, a novel gene translocation, (4p15, 6q22), in human non-small cell lung carcinoma (NSCLC) that results in a fusion proteins combining part of Sodium-dependent Phosphate Transporter Isoform NaPi-3b protein (SLC34A2) with Proto-oncogene Tyrosine Protein Kinase ROS Precursor (ROS) kinase has now been identified. The SLC34A2-ROS fusion protein is anticipated to drive the proliferation and survival of a subgroup of NSCLC tumors. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ROS kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of the new fusion protein enables new methods for determining the presence of these mutant ROS kinase polypeptides in a biological sample, methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides, which are also provided by the invention. | 2013-03-21 |
20130071842 | Hypermethylation Biomarkers for Detection of Head and Neck Squamous Cell Cancer - Differentially methylated oral squamous cell carcinoma (OSCC) biomarkers, identified in-vitro and validated in well-characterized surgical specimens, have shown poor clinical correlation in cohorts with different risk profiles. To overcome this lack of relevance we used the HumanMethylation27 BeadChip, publicly available methylation and expression array data, and Quantitative Methylation Specific PCR to uncover differential methylation in OSCC clinical samples with heterogeneous risk profiles. A two stage-design consisting of Discovery and Prevalence screens was used to identify differential promoter methylation and deregulated pathways in patients diagnosed with OSCC and head and neck squamous cell carcinoma. This Phase I Biomarker Development Trial identified a panel of differentially methylated genes in normal and OSCC clinical samples from patients with heterogeneous risk profiles. This panel may be useful for early detection and cancer prevention studies. | 2013-03-21 |
20130071843 | METHODS, PROBE SETS, AND KITS FOR DETECTION OF DELETION OF TUMOR SUPPRESSOR GENES BY FLUORESCENCE IN SITU HYBRIDIZATION - Methods, probe sets, kits, and compositions for gene deletion assays are disclosed. In some embodiments, the methods relate to preparing probes for a deletion assay, performing a deletion assay, or optimizing a deletion assay. In some embodiments, the methods and probe sets can provide reduced artifactual deletion frequency, for example, when analyzing samples subject to truncation artifacts. In some embodiments, the methods and probe sets can distinguish between small and large deletions. | 2013-03-21 |
20130071844 | METHOD FOR DETECTING TARGET BASE SEQUENCE USING COMPETITIVE PRIMER - Disclosed is a method of detecting a target base sequence having a polymorphic base, the method including: (a) a step of adding to a nucleic acid sample having a target nucleic acid that includes a base sequence including the target base sequence: at least one type of detection primer, at least one type of competitive primer, and at least one type of common primer; (b) a step of annealing the detection primer and the competitive primer to the target nucleic acid in a competitive manner, thereby synthesizing an extension product A; (c) a step of annealing the common primer to the extension product A obtained in the step (b) or in the following step (d), thereby synthesizing an extension product B; (d) a step of annealing the detection primer or the competitive primer to the extension product B obtained in the previous step (c), thereby synthesizing the extension product A; and (e) a step of detecting the extension product A or the extension product B. | 2013-03-21 |
20130071845 | IDENTIFICATION OF HUMAN MOUSE DOUBLE MINUTE 2 HOMOLOG NUCLEOTIDE SEQUENCES - The invention is directed to isolated genomic polynucleotide fragments that encode human carboxypeptidase M and human mouse double minute 2 homolog, vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human carboxypeptidase M and human mouse double minute 2 homolog and to diagnose, treat, prevent and/or ameliorate a pathological disorder. | 2013-03-21 |
20130071846 | KIT AND METHOD - The present invention relates to a kit comprising a DNA-dependant DNA polymerase and at least one natural deoxynucleoside and to a kit comprising a DNA-dependent DNA polymerase and a detection system comprising a DNA template molecule, a DNA primer molecule, and a fluorescent moiety capable of being displaced from,or bound to, dsDNA synthesized by said DNA-dependent polymerase. It further relates to A method for measuring deoxynucleoside kinase activity in a sample characterized by; (i) contacting the sample, in a container, with a reaction mix comprising a DNA-dependent DNA polymerase, at least one deoxynucleoside and a detection system comprising a DNA template molecule, a DNA primer molecule, and a fluorescent moiety capable of being incorporated in, displaced from,or bound to dsDNA synthesized by said DNA dependent polymerase; (ii) incubating said container; (iii) measuring the signal from the fluorescent moiety;and correlating the signal from the fluorescent moietyto the deoxynucleoside kinase activity in the sample. | 2013-03-21 |
20130071847 | METHODS AND MATERIALS FOR DETERMINING THE SOURCE OF WASTE - A method for identifying the source of animal waste is provided. The method includes taking DNA samples from a known group of animals, conducting DNA analysis on the DNA samples to prepare a genetic profile for each animal from the group, preparing a database of the genetic profiles, collecting a specimen of waste from an unknown source, conducting DNA analysis on the specimen, and comparing the DNA analysis from the specimen to the database to determine the source of the waste. | 2013-03-21 |
20130071848 | One-step method for quantitative determination of uracil in DNA by real-time PCR - Uracil may occur in DNA due to either cytosine deamination or thymine replacing incorporation. Its quantitative characterization is important in assessing DNA damages in cells with perturbed thymidylate metabolism or within different DNA segments involved in immunoglobulin gene diversification. The archaeal DNA polymerase from | 2013-03-21 |
20130071849 | FRET-LABELED COMPOUNDS AND USES THEREFOR - FRET-labeled compounds are provided for use in analytical reactions. In certain embodiments, FRET-labeled nucleotide analogs are used in place of naturally occurring nucleoside triphosphates or other analogs in analytical reactions comprising nucleic acids, for example, template-directed nucleic acid synthesis, DNA sequencing, RNA sequencing, single-base identification, hybridization, binding assays, and other analytical reactions. | 2013-03-21 |
20130071850 | WAVEGUIDE-BASED OPTICAL SCANNING SYSTEMS - A scanning sensor system, methods of use and kits for detecting a biologically active analyte are provided. The scanning senor system includes a light source, a detector, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, and at least one adapter configured to couple with the substrate and provide optical communication between the light source, the waveguides of the substrate, and the detector. | 2013-03-21 |
20130071851 | MOVING MICRODROPLETS IN A MICROFLUIDIC DEVICE - The present invention relates to a system and method for moving samples, such as fluid, within a microfluidic system using a plurality of gas actuators for applying pressure at different locations within the microfluidic. The system includes a substrate which forms a fluid network through which fluid flows, and a plurality of gas actuators integral with the substrate. One such gas actuator is coupled to the network at a first location for providing gas pressure to move a microfluidic sample within the network. Another gas actuator is coupled to the network at a second location for providing gas pressure to further move at least a portion of the microfluidic sample within the network. A valve is coupled to the microfluidic network so that, when the valve is closed, it substantially isolates the second gas actuator from the first gas actuator. | 2013-03-21 |
20130071852 | Controls for Detecting Methicillin Resistant Staphylococcus Aureus (MRSA) - The invention relates to the quality control of | 2013-03-21 |
20130071853 | METHODS FOR IDENTIFYING COMPOSITIONS THAT ALTER WILDTYPE EXPRESSION OF GENES AND PROTEINS IN A PLANT CELL - A method for identifying compounds that selectively alter wildtype gene expression of a plant cell includes comparing a first expression profile from a plant cell or tissue contacted with a first test compound, an additional expression profile from the same plant cell or tissue contacted with an additional test compound, and a combined compound expression profile from the same plant cell or tissue contacted with the first test compound and the additional test compound, wherein each plant cell or tissue was contacted for a time sufficient to produce an alteration in the expression of at least one gene or gene product in the plant's cells or tissues; and wherein each expression profile comprises the expression level or pattern of at least one gene or gene product of the plant cell or tissue; and identifying a significant, unexpected alteration in the level or pattern of expression between the genes or gene products of the combined compound profile and those of the additive alterations of the first profile and the additional profile. | 2013-03-21 |
20130071854 | METHODS FOR THE IDENTIFICATION AND CHARACTERIZATION OF HDAC INTERACTING COMPOUNDS - The present invention relates to methods for the identification and characterization (e.g. selectivity profiling) of HDAC interacting compounds using protein preparations derived from cells endogenously expressing HDACs or cell preparations containing said HDACs. | 2013-03-21 |
20130071855 | FLC AS BIOMARKER - The invention provides a method of identifying a subject likely to have liver disease, or for determining the prognosis of a subject previously identified as having a liver disease comprising detecting an amount of free light chains in a sample from the subject, wherein a higher amount of FLC is associated with an increased likelihood of the subject having a liver disease or an increased likelihood of having a poor prognosis of a liver disease. Assay kits for use in such methods are also provided. | 2013-03-21 |
20130071856 | METHOD AND BIOMARKER FOR EVALUATING METASTASIS, AND SIRNA COMPOUND FOR INHIBITING METASTASIS - A method and biomarker for evaluating metastasis, and an siRNA compound for inhibiting metastasis. The method of the present invention includes: providing a sample of a subject, which includes a normal tissue and a tissue to be detected; detecting expression of a biomarker of the normal tissue and the tissue to be detected, respectively, wherein the biological marker is SERPINA1; and comparing the expression of the biological marker of the normal tissue and the tissue to be detected. When the expression of the biological marker of the tissue to be detected is higher than the normal tissue, it represents that the subject is at a risk of suffering from metastasis. | 2013-03-21 |
20130071857 | Biomarkers Of Gastric Cancer And Use Thereof - A set of biomarkers indicative of early gastric cancer and method of diagnosing gastric cancer at an early stage by directing the these biomarkers in a blood sample. Detection of over-expression of one or more protein biomarks in the group consisting albumin, T-kininogen I, α-2-HS glycoprotein, α-1-antitrypsin, afamin and γ-actin and/or detection of under-expression of one or more protein biomarks in the group consisting stress 70 protein, apolipoprotein A-I, apolipoprotein A-IV, transthyretin and murinoglobulin is indicative of the presence of gastric cancer. | 2013-03-21 |
20130071858 | AUTOMATED STAINING SYSTEM AND REACTION CHAMBER - An apparatus including a reagent cartridge and a reaction chamber, the reagent cartridge having a reagent capsule removably positioned therein for dispensing of a reagent onto the reaction chamber. A system including a linearly translatable mounting assembly having a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge, a linearly translatable bulk reagent dispensing assembly having a plurality of bulk reagent dispensing nozzles coupled thereto and a receiving assembly positioned beneath the mounting assembly and the bulk reagent dispensing assembly, the receiving assembly including a plurality of reaction stations. A method including determining an inventory of an automated sample processing system, downloading a processing protocol from a central controller to the automated sample processing system, operating the automated sample processing system based on the processing protocol and independently of the central controller and dispensing a reagent from the automated sample processing system. | 2013-03-21 |
20130071859 | Methods of Determining Patient Response by Measurement of HER-3 - The invention provides methods of measuring and/or quantifying the presence and/or amount of Her-3 and/or Her-3 in a complex in a sample. The invention also provides antibodies specific for Her-3. | 2013-03-21 |
20130071860 | METHODS FOR AUTOIMMUNE DISEASE DIAGNOSIS, PROGNOSIS, AND TREATMENT` - In one aspect, the present invention provides methods for the classification, diagnosis, prognosis, theranosis, and/or prediction of an outcome of an autoimmune disease in a subject. | 2013-03-21 |
20130071861 | METHODS AND KITS FOR DETECTING ITA IN A BIOLOGICAL SAMPLE - Methods for detecting invasive trophoblast antigen (ITA) in biological samples comprise screening the samples for ITA using antibodies that bind to the ITA. The methods are useful to detect pregnancy, trophoblastic diseases, and Down's syndrome in fetuses of pregnant women. Some methods include screening the samples with a plurality of capture antibodies that specifically bind ITA. Chemiluminescent immunoassays are disclosed. The methods may be practiced with the diagnostic kits of the invention. | 2013-03-21 |
20130071862 | FUSION PROTEINS AND THEIR USE IN THE DIAGNOSIS AND TREATMENT OF LEISHMANIASIS - The present invention relates generally to a fusion protein made from a synthetic gene construct comprising of elements derived from the | 2013-03-21 |
20130071863 | Sulfur-Containing Amino Acid Derivative - The present invention provides a method of measuring an endogenous low-molecular-weight compound specifically and conveniently with high sensitivity. Using the particular sulfur-containing amino acid derivative, a method of measuring an endogenous low-molecular-weight compound specifically and conveniently with high sensitivity can be provided. | 2013-03-21 |
20130071864 | COLORECTAL CANCER MARKER VITRONECTIN AND METHOD FOR ANALYZING VITRONECTIN CONCENTRATION IN BLOOD SAMPLE - The present invention provides a tumor screening marker that can be actually used in clinical practice to detect colorectal cancer, and a tumor progression marker that can complement CEA or CA | 2013-03-21 |
20130071865 | Devices And Methods For Detecting Amniotic Fluid In Vaginal Secretions - The present invention relates to a diagnostic method for the detection of small quantities of amniotic fluid in the vagina. More specifically, the invention relates to the detection of PAMG-1 in the vagina using anti-PAMG-1 antibodies. | 2013-03-21 |
20130071866 | METHOD FOR TESTING THE SEVERTIY OF AN ILLNESS - An object of the present invention is directed to a method for assaying the severity of an illness in real time and is to provide a testing method capable of assessing the severity of an illness in more detail than the conventional APACHE II and SOFA scores. The established method can accurately measure an ATP level in a sample, thereby accurately and quickly deducing the “state of intracellular energy required for living organisms” from the ATP level, and by extension, determining the severity of an illness. The present invention further provides a novel biomarker ATP-lactate energy risk score (A-LES) value that is capable of determining the severity of an illness by the reevaluation, with the ATP concentration as an index (specifically, on the basis of a lactic acid level (mM)/ATP concentration (mM) ratio), of the level of lactic acid that accumulates in the sample due to the breakdown of in vivo metabolic balance accompanied by the increased severity of the illness. The present invention also provides a novel biomarker ATP-ketone energy risk score (A-KES) value that is capable of determining the severity of an illness by the reevaluation of a ketone body level in the sample with the ATP concentration as an index (specifically, on the basis of a ketone body level (mM)/ATP concentration (mM) ratio). | 2013-03-21 |
20130071867 | Method And Apparatus For Performing Mass Spectrometry - The invention feature devices and methods for preserving and processing samples comprising a fluid having deuterated compounds. The device of the present invention comprises a housing defining a first chamber and a second chamber. The first chamber is heated to an elevated temperature and receives the sample and performs a digestion process on the sample at the elevated temperature. The second chamber is cooled to a low temperature and receives the deuterated digested sample from the first chamber and performs one or more separation steps to isolate an analyte. The device of further comprises conduit means for containing and moving the sample into the first chamber to form a digested sample. The conduit means moves the digested sample from the first chamber to the second chamber to separate the sample to form at least one analyte. The analyte is maintained at the low temperature to preserve its deuterated form. | 2013-03-21 |
20130071868 | MUTANT LACTATE OXIDASE WITH INCREASED STABILITY AND PRODUCT, METHODS AND USES INVOLVING THE SAME - The present disclosure relates to a mutant lactate oxidase having increased stability, a nucleic acid encoding the mutant lactate oxidase, an expression vector comprising the nucleic acid, a host cell comprising the nucleic acid or the expression vector, a method of determining lactate in a sample, the use of the mutant lactate oxidase for determining lactate, a device for determining lactate in a sample using the mutant lactate oxidase and a kit for determining lactate comprising the mutant lactate oxidase. | 2013-03-21 |
20130071869 | Analysis Compensation Including Segmented Signals - A biosensor system determines analyte concentration from an output signal generated from a light-identifiable species or a redox reaction of the analyte. The biosensor system compensates at least 50% of the total error in the output signal with a primary function and may compensate a portion of the residual error with at least one residual function. An SSP function may serve as the primary function, first residual function, or second residual function. Preferably, when the SSP function serves as the first residual function, the SSP function compensates at least 50% of the residual error remaining after primary compensation. Preferably, when the SSP function serves as the second residual function, the SSP function compensates at least 50% of the residual error remaining after primary and first residual compensation. The error compensation provided by the primary, first residual, and second residual functions may be adjusted with function weighing coefficients. | 2013-03-21 |
20130071870 | Method for Determining the Stability of Organic Methyleneamines in the Presence of Semicarbazide-Sensitive Amine Oxidase - The present invention provides methods for determining the stability of methyleneamine, methyleneamine-like compounds or compounds containing a methyleneamine moiety in the presence of semicarbazide-sensitive amine oxidase (SSAO) or a biological sample containing SSAO activity. The disclosed methods may be configured in an assay format for high throughput screening applications. | 2013-03-21 |
20130071871 | ENZYMATIC DETERMINATION OF LITHIUM IONS USING PHOSPHOGLUCOMUTASE - Kits and methods for measuring lithium ions using phosphoglucomutase are disclosed. | 2013-03-21 |
20130071872 | DEVICE AND METHOD FOR SINGLE-USED MATRIX SAMPLING - A single-used matrix sampling device for the single-used, disposable or autoclavble packed-bed bioreactor is provided herein. The present invention includes a tube means and a matrix collecting means. The matrix collecting means configured for collecting the cell culture matrix includes a receptacle and a pulling means connected to the receptacle or the matrix collecting means could be scoop-like in configuration; the tube means is configured for preventing the matrix collecting means from being contaminated while mechanically or pneumatically sampling; and the tube means could be sealed to enclose the sampled matrix collecting means after sampling. A single-used matrix sampling method for a single-used, disposable or autoclavble packed-bed bioreactor is also provided. The present invention could be utilized to aseptically collect the cell culture matrix from the packed-bed bioreactor without requiring to face the risks of contamination. | 2013-03-21 |
20130071873 | ARRAY MICROINJECTION APPARATUSES AND METHODS - An array microinjection apparatus comprises a substrate ( | 2013-03-21 |
20130071874 | SYSTEM AND METHOD FOR ANTI-CANCER DRUG CANDIDATE EVALUATION - The disclosure provides a method of evaluating the ability of an anti-cancer drug candidate to induce apoptosis in a known cancer cell line by placing a single-cell suspension of a known cancer cell line in a well of a plate, adding at least one drug candidate to the well in an amount sufficient to achieve a target drug candidate concentration, measuring the optical density at selected time intervals for a selected duration of time, determining a kinetic units value from the optical density and time measurements, and correlating the kinetic units value with an ability of the anti-cancer drug candidate to induce apoptosis in the cancer cell line if the kinetic units value is positive. A similar method may be used to evaluate the ability of an anti-cancer drug candidate to induce apoptosis in a cancer type. | 2013-03-21 |
20130071875 | METHOD AND SYSTEM FOR THE DETECTIONS OF BIOLOGICAL OBJECTS - The present invention provides a method for identifying biological objects on a substrate; the method comprises: (i) illuminating the substrate carrying the biological objects with a light beam; (ii) acquiring at least one optical image of the illuminated substrate; the light beam comprises a wavelength band, the wavelength band is selected to induce at least one optical aberration in the optical image of the illuminated substrate; the optical aberration is predetermined as characterizing the biological objects; and (iii) processing said at least one optical image to provide a value or a combination of values indicative of presence or absence of said at least one optical aberration; said value or combination of values permitting detection or identification of the biological objects on said substrate. The invention also provides a processing unit, a system and a database for use in connection with the method of the invention. | 2013-03-21 |
20130071876 | Method for Utilizing Soft X-Ray Microimaging for Cancer Cell Image Recognition - This invention discloses a method for utilizing soft X-ray microimaging for cancer cell image recognition. The method comprises the steps of 1) sample preparation; 2) pathological examination; 3) soft X-ray imaging; and 4) analysis and recognition. This invention applies soft X-ray microimaging for cancer cell image recognition, successfully obtains the soft X-ray microscopic image of a cancer cell by scanning the cancer cell with synchrotron radiation soft X-ray microimaging, provides recognition steps and experimental data, and establishes a method for utilizing soft X-ray microimaging for cancer cell image recognition. This invention creates a method for analyzing soft X-ray microscopic images, provides a novel synchrotron radiation soft X-ray pathological diagnosis method for cancer diagnosis, and provides an extremely valuable basis for the creation and clinical application of soft X-ray pathology in the 21 | 2013-03-21 |
20130071877 | Chimeric isoprenoid synthases and uses thereof - Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide. | 2013-03-21 |
20130071878 | TWO HELIX BINDERS - Provided herein are isolated polypeptides derived from the staphylococcal protein A protein B domain comprising a pair of anti-parallel alpha helices that are capable of binding a target. Also provided are nucleic acid sequences encoding such two helix binders, vectors containing the nucleic acid sequences encoding for two helix binders, and host cells transformed with vectors containing the nucleic acid sequences encoding for the two-helix binders. Also provided are methods of using the two helix binders. | 2013-03-21 |
20130071879 | AMPLIFICATION METHODS TO MINIMISE SEQUENCE SPECIFIC BIAS - Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimising GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilising flow cells. | 2013-03-21 |
20130071880 | Dna amplification apparatus and method - The invention relates to a method for the amplification of DNA and apparatus for such amplification. The method comprises steps typically found in amplification methods but utilises an optical procedure to detect denaturation of DNA or attain | 2013-03-21 |
20130071881 | METHODS FOR PRODUCING ANTIBODY-PRODUCING CELLS THAT PRODUCE DESIRED POLYPEPTIDES - An objective of the present invention is to provide methods for introducing DNAs encoding a desired amino acid sequence into a region comprising a DNA encoding an antibody variable region of antibody-producing cells. The present inventors developed methods for efficiently introducing DNAs encoding a desired amino acid sequence into the antibody variable region gene locus of DT40-SW, which is a mutant line of the DT40 chicken B cell line which has the ability to spontaneously introduce mutations. This allows mutagenesis of introduced DNAs to modify the polypeptides to have superior functions. In particular, the present inventors revealed the nucleotide sequence of the antibody H chain variable region gene locus of the DT40 cell line. Based on this finding, the present inventors successfully constructed targeting vectors that allow efficient substitution of the antibody H chain variable region gene locus of the DT40 cell line with a gene encoding a desired polypeptide. | 2013-03-21 |
20130071882 | COLLECTIVE CHIRALITY OF BINARY PLASMONIC NANOPARTICLES JANUS ASSEMBLIES - Multiple properties of plasmonic assemblies are determined by their geometrical organization. This patent focuses on the formation of Janus structure of the asymmetric assembly structure of the gold nanorods and gold nanoparticles. Chiral structure of gold nanorods and gold nanoparticles can be obtained through the characterization of optical spectra of the Janus structure. And it opens the door for the explanation of the mechanism of the chirality, plays a strong guiding role in the negative refractive material above and has good application prospects. | 2013-03-21 |
20130071883 | METHODS AND ORGANISMS FOR UTILIZING SYNTHESIS GAS OR OTHER GASEOUS CARBON SOURCES AND METHANOL - The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO | 2013-03-21 |
20130071884 | PLANTS EXPRESSING CELL WALL DEGRADING ENZYMES AND EXPRESSION VECTORS - Vectors for expression of proteins in plants are described. The proteins may be enzymes and the enzymes can be but are not limited to cell wall degrading enzymes. A number of plants designed to express specific cell wall degrading enzymes are provided. The plants may have industrial and/or agricultural applications. Methods and materials for making the expression vectors and for making the plants are provided. Processes for which the plants could be used in industrial and agricultural applications are also provided. | 2013-03-21 |
20130071885 | ACYL AMINO ACIDS - Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides. In certain embodiments, an acyl amino acid produced using compositions and/or methods of the present invention comprises cocoyl glutamate. | 2013-03-21 |
20130071886 | MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL - The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO. | 2013-03-21 |
20130071887 | ENZYMATIC CLEAVAGE OF STEVIOSIDE TO PRODUCE STEVIOL - This invention is directed to a process for cleaving glycoside residues from stevioside to produce steviol using a commercially available enzyme mixture containing pectinase, CYTOLASE PCL5®. In preferred methods, the reaction takes place in the presence of a yeast culture. Also described are combinations of | 2013-03-21 |
20130071888 | METHOD FOR PRODUCING 1,5-PENTANEDIAMINE - A method of producing 1,5-pentanediamine includes culturing coryneform bacterium having a gene encoding lysine decarboxylase in its chromosome, which coryneform bacterium maintains lysine decarboxylase activity of not less than 50 mU/mg protein during culturing and the gene encoding lysine decarboxylase is linked downstream of a promoter that functions during the logarithmic growth phase. | 2013-03-21 |
20130071889 | PRODUCTION METHOD FOR BIOFUEL - The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S | 2013-03-21 |
20130071890 | Production of PHA using Biogas as Feedstock and Power Source - Methods for producing bioplastics from biogas include techniques for the production of PHB using a dirty biogas (e.g., methane from landfill, digester) as both a power source for the process and as feedstock. Biogas is split into two streams, one for energy to drive the process, another as feedstock. Advantageously, the techniques may be implemented off the power grid with no dependence upon agricultural products for feedstock. | 2013-03-21 |
20130071891 | IN SITU EXPRESSION OF LIPASE FOR ENZYMATIC PRODUCTION OF ALCOHOL ESTERS DURING FERMENTATION - Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity. | 2013-03-21 |
20130071892 | PROCESS FOR PRODUCTION OF POLYHYDROXYALKANOIC ACID USING GENETICALLY MODIFIED MICROORGANISM HAVING ENOYL-CoA HYDRATASE GENE INTRODUCED THEREIN - The present invention intends to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] with a high 3-hydroxyhexanoic acid fraction using a vegetable oil as a basic raw material. In accordance with the present invention, there is provided a method of producing a microorganism that produces poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a high 3-hydroxyhexanoic acid fraction using a vegetable oil as a basic raw material, by introducing a gene encoding R-hydratase that converts a fatty acid β-oxidation system intermediate to a monomer, (R)-3-hydroxyacyl-CoA [R-3HA-CoA], into a recombinant | 2013-03-21 |
20130071893 | METHODS FOR PRODUCING 3-HYDROXYPROPIONIC ACID AND OTHER PRODUCTS - This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid. | 2013-03-21 |
20130071894 | GLUCOSE VALVE AND OTHER METABOLITE VALVES - Aspects of the invention relate to the design and construction of Metabolite Valves, such as Glucose Valves, that can be used to divert metabolites from endogenous pathways toward alternative pathways in a cell. | 2013-03-21 |
20130071895 | L-LACTATE PRODUCTION IN CYANOBACTERIA - A process of producing L-lactate as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting said culture to light, wherein said cell is capable of expressing a nucleic acid molecule, wherein the expression of said nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into L-lactate and wherein said nucleic acid molecule is under the control of a regulatory system which responds to light or to a change in the concentration of a nutrient in said culture. | 2013-03-21 |
20130071896 | METHOD FOR CONTROLLING UNDESIRABLE BYPRODUCTS FORMATION CAUSED BY CONTAMINATING ORGANISMS IN THE PRODUCTION OF ETHANOL FROM SYNGAS - A method of operating a fermentation zone for the production of ethanol from syngas uses a crotonate derivative to prevent or reverse the effects of butyrigen contamination. The crotonate compound works in continuous fermentation processes to reduce or eliminate contamination from butyrate and butanol in the syngas derived ethanol product. | 2013-03-21 |
20130071897 | METHOD FOR CONTROLLING UNDESIRABLE BYPRODUCTS FORMATION CAUSED BY CONTAMINATING ORGANISMS IN THE PRODUCTION OF ETHANOL FROM SYNGAS - A method of operating a fermentation zone for the production of ethanol from syngas uses a crotonate-like compound to prevent or reverse the effects of butyrogen contamination. The crotonate-like compound works in continuous fermentation processes to reduce or eliminate contamination from butyrate and butanol in the syngas derived ethanol product. | 2013-03-21 |
20130071898 | HOST CELLS AND METHODS FOR PRODUCTION OF ISOBUTANOL - Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity. | 2013-03-21 |
20130071899 | Process for Producing Aliskiren - A new route of synthesis of the compound Aliskiren of formula (I), used in the treatment of hypertension, is described. | 2013-03-21 |
20130071900 | PROCESS FOR PROCESSING A LIGNOCELLULOSIC MATERIAL - Calcium-containing lignocellulosic material is converted at a temperature in the range from equal to or more than 120° C. to equal to or less than 210° C. with an aqueous acid solution containing one or more inorganic acids and having a pH in the range from equal to or more than 1.8 to equal to or less than 4.0 to produce a mixture, containing pretreated lignocellulosic material and aqueous acid solution, having an overall pH in the range from equal to or more than 3.0 to equal to or less than 4.5. The process allows reduction in the formation of insoluble salts. | 2013-03-21 |
20130071901 | PROCESS FOR THE CONVERSION OF A XYLAN-CONTAINING FEED - A xylan-containing feed is contacted, in the first reactor essentially operated at plug flow, with an aqueous solution of an acid at a temperature in the range from equal to or more than 140° C. to equal to or less than 210° C. to produce an intermediate product. Then, the intermediate product is contacted, in the second reactor which comprises a continuously stirred tank reactor, with an aqueous solution of an acid at a temperature in the range from more than 130° C. to equal to or less than 200° C. to produce a final product. | 2013-03-21 |
20130071902 | METHOD AND SYSTEM FOR COLLECTING WATER FROM AN AQUATIC CELL - Methods and systems for collecting, purifying, and/or extracting ethanol produced during anaerobic metabolism by aquatic plants is provided. The system includes a cell containing water and an aquatic plant, an ethanol extraction assembly in fluid communication with the cell for removing ethanol from the water. Ethanol is released by the aquatic plant by initiating an anaerobic process in the plant such as by regulating the photosynthesis inducing light that reaches the aquatic plant. | 2013-03-21 |
20130071903 | METHOD FOR HEATING A FEEDSTOCK - The present invention provides a method for producing a pretreated or hydrolyzed lignocellulosic feedstock. The method comprises feeding a lignocellulosic feedstock to a plug formation device and forming a feedstock plug therein. The plug or segments thereof are fed into an elongate chamber that comprises steam addition means for direct steam addition and a rotating shaft mounted co-axially within the chamber having one or more disintegrating elements mounted on it. Disintegrated feedstock particles are produced in the elongate chamber by the disintegrating elements. The disintegrated feedstock particles are heated by contact with the steam introduced through the steam addition means. The disintegrated feedstock particles are then treated in a reactor to produce the pretreated or hydrolyzed lignocellulosic feedstock. Further provided is a feedstock composition comprising disintegrated feedstock particles. Also provided are methods for reducing erosion on equipment by maintaining the discharge consistency from the plug formation device below 35 wt %. | 2013-03-21 |
20130071904 | ACETATE-RESISTANT YEAST STRAIN FOR THE PRODUCTION OF A FERMENTATION PRODUCT - The present invention provides methods and compositions for fermentations comprising acetate-resistant yeast. The present invention provides methods for use of acetate-resistant yeast for the production of fermentation products. | 2013-03-21 |
20130071905 | PLASMA IRRADIATION DEVICE FOR SUBSTANCE INTRODUCTION AND SUBSTANCE INTRODUCTION METHOD USING PLASMA IRRADIATION DEVICE - Disclosed is a liquid culture medium for substance introduction, which is capable of increasing the survival rate of cells after substance introduction as much as possible when the cells are irradiated with plasma for the purpose of introducing a target substance into each of the cells. Specifically disclosed is a liquid culture medium for substance introduction, which is used for the purpose of introducing a predetermined target substance into a cell and enables introduction of the target substance into the cell by having the cell in the liquid culture medium, which contains the target substance, irradiated with a plasma jet. The liquid culture medium contains a damage preventing component that prevents the cell from damage due to the plasma jet. | 2013-03-21 |
20130071906 | CELL CULTURE MEDIUM FOR ADAMTS PROTEIN EXPRESSION - The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein. | 2013-03-21 |
20130071907 | BILE RESISTANT BACILLUS COMPOSITION SECRETING HIGH LEVELS OF PHYTASE | 2013-03-21 |
20130071908 | COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE - The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. | 2013-03-21 |
20130071909 | Heterotrophic Cultivation of Hydrocarbon-Producing Microalgae - The invention discloses novel methods of producing hydrocarbons through heterotrophic cultivation of | 2013-03-21 |
20130071910 | STORAGE-STABLE LIQUID DETERGENT OR CLEANING AGENT CONTAINING PROTEASE AND LIPASE - The object is to improve the storage stability of a liquid washing or cleaning agent comprising a protease and lipase with regard to lipolytic activity. This is achieved through the use of a protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence set out in SEQ ID NO. 1 and that has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the sequence corresponding to SEQ ID NO. 1. | 2013-03-21 |
20130071911 | METHOD FOR PAPER TREATMENT WITH ENZYME - Exemplary embodiments provide methods for treating a xerographic printed substrate with an enzyme solution to increase the surface free energy of the substrate. Other exemplary embodiments provide methods for making an adhesive medium with an enzyme-treated substrate. | 2013-03-21 |
20130071912 | METHODS AND APPARATUS OF CONTROLLING SURFACE WETTABILITY OF XEROGRAPHIC PRINTS - Exemplary embodiments provide methods and apparatus of using an enzyme-surfactant solution to control surface wettability of a xerographic print. | 2013-03-21 |
20130071913 | USE OF AMYLASE VARIANTS AT LOW TEMPERATURE - The present invention relates to the use of alpha-amylase variants having improved activity relative to the parent enzyme at low temperature, including improved washing and/or dishwashing performance and/or increased stability at low temperature. The invention further relates a method for doing laundry, dish wash and/or cleaning such as institutional cleaning and to compositions for use at low temperature. | 2013-03-21 |
20130071914 | METHOD FOR STUDYING FLOATING, LIVING CELLS - Disclosed is a method of studying floating living cell by confining the cells floating in an aqueous medium in picowells located on a bottom surface of a top wall of a chamber. Disclosed is also a picowell-bearing device for the study of cells comprising a chamber having at least one picowell on a bottom surface of the chamber. Also disclosed is a picowell-bearing device for the study of cells comprising a chamber having at least one picowell on an internal surface and at least one moveable wall, a force applicator configured to removeably apply a substantially uniformly distributed force around the periphery of the moveable wall in a direction to retain the moveable wall secured to the chamber. | 2013-03-21 |
20130071915 | MICROWELL STRUCTURES FOR CHEMICALLY-SENSITIVE SENSOR ARRAYS - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 2013-03-21 |
20130071916 | SYNTHETIC COATING FOR CELL CULTURE - A method for coating a surface of a cell culture article includes dissolving a polymer having a covalently attached polypeptide in an aqueous solution to produce a polymer solution. The polymer is formed from monomers selected to form a polymer having a linear backbone, wherein the polymer is crosslink free. The weight percentage of the polypeptide relative to the polymer conjugated to the polypeptide is sufficiently high to render the polymer conjugated to the polypeptide water soluble. The aqueous solution is substantially free of organic solvents. The method further includes (i) disposing the polymer solution on the surface of the cell culture article to produce a coated article; and (ii) subjecting the coated article to sufficient heat or electromagnetic radiation to attach the polymer conjugated to a polypeptide to the surface of the cell culture article. | 2013-03-21 |
20130071917 | CAPILLARY FOR APPARATUS OF INSULATED ISOTHERMAL POLYMERASE CHAIN REACTION - A capillary for apparatus of insulated isothermal polymerase chain reaction is mounted onto a test tube holder. The capillary includes a tube which has an elongated tubular housing space that is cut to form an axial cross section with a distance between left and right edges greater than the distance between front and rear edges. When reactants are filled in the elongated tubular housing space and heated to generate convection, the left and right edges of the axial cross section form a narrower passage to reduce flowing speed of heat flow in the convection and increase cycling duration of the convection. Hence reaction duration of the reactants is prolonged, and reaction efficiency of the apparatus of insulated isothermal polymerase chain reaction also is increased. | 2013-03-21 |
20130071918 | CELL CULTURE CONTAINER AND METHOD FOR FABRICATING THE SAME - The present invention relates to a cell culture container, which enhances the efficiency of proliferation and differentiation of various cells including stem cells. The cell culture container according to an exemplary embodiment of the present invention includes a cell culture surface for adhering adult stem cells thereon to proliferate and differentiate the adult stem cells, and the cell culture surface includes a protrusion having a lotus leaf surface reproduction structure, which is disposed on the cell culture surface. | 2013-03-21 |
20130071919 | METHOD OF SELECTING INDUCED PLURIPOTENT STEM CELL - The present invention provides a method of selecting a highly safe induced pluripotent stem cell, which includes comprehensively detecting the sequence of an expression vector used for induction of the induced pluripotent stem cell, in the nucleic acid in the cell, and a kit used for the method. | 2013-03-21 |
20130071920 | Mesenchymal Stem Cell Attractant and Method for Attracting Mesenchymal Stem Cell - The present invention aims to provide a mesenchymal stem cell attractant capable of attracting a mesenchymal stem cell; and others. The present invention provides a mesenchymal stem cell attractant containing at least one selected from the group consisting of an extract of a | 2013-03-21 |
20130071921 | ANTIBODIES SPECIFIC FOR DKK-1 AND THEIR USES - The present invention provides antibodies and fragments thereof that bind to Dkk-1 and, in particular, to humanized antibodies and fragments thereof that bind to Dkk-1 and, even more particularly to fully humanized antibodies and immunologically functional fragments that bind to Dkk-1. Also provided are antibodies and fragments thereof which compete with the binding of an anti-mouse Dkk-1 monoclonal antibody for binding to Dkk-1 | 2013-03-21 |
20130071922 | Anti-C5 Alpha Antibodies - The present invention refers to recombinant antibodies of human origin specific for the C5 component of the activated complement and characterised by the ability to inhibit the conversion of the C5 alpha chain to C5a and C5b. Moreover the present invention refers to the nucleotide sequences coding for such antibodies and to the therapeutic use of both polypeptide and nucleotide sequences, in particular for the therapy of diseases involving tissue damage deriving from uncontrolled activation of the complement system. | 2013-03-21 |
20130071923 | HUMAN GM-CSF ANTIGEN BINDING PROTEINS - Antigen binding proteins that bind to human GM-CSF protein are provided. Nucleic acids encoding the antigen binding protein, vectors, and cells encoding the same are also provided. The antigen binding proteins can inhibit binding of GM-CSF to GM-CSFR, inhibit GM-CSF-induced proliferation and signaling of myeloid lineage cell lines and inhibit GM-CSF-induced activation of human monocytes. | 2013-03-21 |
20130071924 | CELLS EXHIBITING NEURONAL PROGENITOR CELL CHARACTERISTICS AND METHODS OF MAKING THEM - Disclosed are cells exhibiting neuronal progenitor cell characteristics, and methods of making them from marrow adherent stem cells by regulating cellular pathways in the marrow adherent stem cells that are associated with glial transdifferentiation of the marrow adherent stem cells. | 2013-03-21 |
20130071925 | CELL CULTURE MEDIUM - The present invention relates to a cell culture medium for culturing human cells comprising an anti-coagulated total blood material wherein the hemoglobin level is from about 8 to about 16 g/dl. More particularly, the invention provides a cell culture medium in which cells present in the blood are disrupted and the insoluble remnants of the lysated cells are removed. Further, the invention provides a method for the preparation of a cell culture medium for culturing human cells, according to the invention. | 2013-03-21 |
20130071926 | COMPOSITIONS AND METHODS FOR GROWING HUMAN EMBRYONIC CELLS - Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing secreted products obtained from the culture medium of human embryonic germ (EG) cell derivatives, such as embryoid body-derived cells. Substrates include compounds such as collagen I, fibronectin, or superfibronectin, or extracellular matrix, typically human derived. | 2013-03-21 |
20130071927 | MEDIA AND METHODS FOR CELL CULTURE - The present invention provides a cell passaging medium comprising at least one agent capable of detaching from a surface a cell that is culture in vitro on said surface, and a water-soluble polymer capable of protecting the detached cell. The present invention also provides a cell culturing medium comprising one or more cell culture protectants capable of protecting cells in culture. The present invention further relates to the use of said media in methods for culturing cells in vitro or for deriving monolayer cell cultures of mammalian stem cells. | 2013-03-21 |
20130071928 | NOVEL SHRNA MOLECULES AND METHODS OF USE THEREOF - The present invention relates to certain novel shRNA molecules and methods of use thereof. According to certain embodiments of the present invention, methods for reducing the expression level of a target gene are provided. Such methods generally comprise providing a cell with one or more precursor nucleic acid sequences that encode two or more RNA molecules. A first RNA molecule comprises a double stranded sequence, which includes a guide strand sequence that is complementary to a portion of an mRNA transcript encoded by the target gene. In addition, a second RNA molecule comprises a second double stranded sequence, which includes a second guide strand sequence that is partially complementary to a portion of the mRNA transcript encoded by the target gene. Preferably, the second guide strand sequence comprises one or more bases that are mismatched with a nucleic acid sequence of the mRNA transcript encoded by the target gene. | 2013-03-21 |
20130071929 | Remedy for Chronic Inflammation and Antibody to be Used Therein - The invention provides a remedy for chronic inflammation and an anti-TNIIIA2 antibody to be used therein. The remedy includes an antibody recognizing TNIIIA2, that is a peptide derived from a partial sequence A2 of a human tenascin-C fibronectin III-like repetitive sequence and having the amino acid sequence RSTDLPGLKAATHYTITIRGVC (SEQ ID NO: 1). | 2013-03-21 |
20130071930 | POLYESTERS, METHODS OF MAKING POLYESTERS AND USES THEREFOR - Polyester compositions and functionalized polyester compositions are provided along with methods of making the compositions as well as methods of using the compositions, for example as a tissue engineering bioscaffold and as a drug-delivery vehicle. | 2013-03-21 |