04th week of 2016 patent applcation highlights part 28 |
Patent application number | Title | Published |
20160024520 | CLONING AND USE OF THE MS9 GENE FROM MAIZE - Nucleotide sequences, amino acid sequences, and an associated promoter sequence are useful in methods of controlling male fertility in plants. Recombinant expression cassettes, vectors, plant cells, and plants comprise a disclosed nucleotide sequence and may encode a disclosed amino acid sequence. The recombinant expression cassettes are useful in controlling fertility, especially male fertility of annual crops. The promoter is useful in driving expression of an operably-linked heterologous polynucleotide. Constructs comprising homology to the promoter sequence are useful for downregulation of a polynucleotide associated with the promoter. | 2016-01-28 |
20160024521 | RETROGRADE DELIVERY OF SDF-1 FOR TREATMENT OF MYOCARDIAL INFARCTION - Described herein are methods of treating a subject with a cardiomyopathy by administering to the subject's heart, via percutaneous retrograde coronary sinus perfusion, a pharmaceutical composition that comprises a DNA plasmid encoding SDF-1 and a pharmaceutically acceptable carrier or diluent. | 2016-01-28 |
20160024522 | NOVEL NUCLEIC ACID MOLECULES - There is provided inter alia a DNA construct which comprises a tRNApyl coding sequence and a RNA polymerase III promoter sequence which is capable of acting to express functional tRNApyl sufficiently to support nonsense suppression in a eukaryotic expression system. | 2016-01-28 |
20160024523 | Using Truncated Guide RNAs (tru-gRNAs) to Increase Specificity for RNA-Guided Genome Editing - CRISPR-Cas genome editing uses a guide RNA, which includes both a complementarity region, which binds the target DNA by base-pairing, and a Cas9-binding region, to direct a Cas9 nuclease to a target DNA. Further disclosed are methods for increasing specificity of RNA-guided genome editing using CRISPR/Cas9 systems by using truncated guide RNAs (tru-gRNAs). | 2016-01-28 |
20160024524 | Increasing Specificity for RNA-Guided Genome Editing - Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems. | 2016-01-28 |
20160024525 | REVERSE GENETICS USING NON-ENDOGENOUS POL I PROMOTERS - Expression of a transgene is driven in a host cell using a pol I promoter which is not endogenous to an organism from the same taxonomic order from which the host cell is derived. | 2016-01-28 |
20160024526 | METHODS AND PHARMACEUTICAL COMPOSITION FOR THE TREATMENT AND THE PREVENTION OF CARDIOMYOPATHY DUE TO ENERGY FAILURE - The present invention relates to a method for preventing or treating cardiomyopathy due to energy failure in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a vector which comprises a nucleic acid sequence of a gene that can restore energy failure. More particularly, the invention relates to a method for preventing or treating a cardiomyopathy associated with Friedreich ataxia in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a vector which comprises a frataxin (FXN) encoding nucleic acid. | 2016-01-28 |
20160024527 | Compositions and Methods for Enhancing Bioenergetic Status in Female Germ Cells - Compositions and methods comprising bioenergetic agents for restoring the quality of aged oocytes, enhancing oogonial stem cells or improving derivatives thereof (e.g., cytoplasm or isolated mitochondria) for use in fertility-enhancing procedures, are described. | 2016-01-28 |
20160024528 | SOMATIC CELL NUCLEAR TRANSFER METHODS - The present invention provides methods for making reconstructed diploid human oocytes comprising the diploid genome of a human somatic cell, and also methods for making human nuclear transfer embryos, human embryonic stem cells, and human differentiated cells therefrom. The present invention also provides reconstructed human oocytes, human nuclear transfer embryos, human embryonic stem cells, and differentiated cells made using such methods, as well as compositions and kits useful in performing such methods. | 2016-01-28 |
20160024529 | METHODS OF IN VIVO ENGINEERING OF LARGE SEQUENCES USING MULTIPLE CRISPR/CAS SELECTIONS OF RECOMBINEERING EVENTS - The present invention provides a method for making a large nucleic acid having a defined sequence in vivo. The method combines recombineering techniques with a CRISPR/Cas system to permit multiple insertions of defined sequences into a target nucleic acid at one time, double stranded cleavage of target nucleic acids in which the defined sequences were not successfully inserted, and selection of successful recombinant cells. The method further includes repeating the process one or more times, using a successful recombinant from one round as the host cell for the next round. | 2016-01-28 |
20160024530 | Cytochrome P450S and Uses Thereof - The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds. | 2016-01-28 |
20160024531 | METHODS USING PERACIDS FOR CONTROLLING CORN ETHANOL FERMENTATION PROCESS INFECTION AND YIELD LOSS - A process for the use of peracid compositions to eliminate and/or control the growth of undesirable bacteria, including contaminating bacteria, in the fermentation production of alcohol is disclosed. Beneficially, the peracid compositions and methods of use of the same do not interfere or inhibit the growth or replication of yeast and have low or no adverse environmental impact. | 2016-01-28 |
20160024532 | ATP DRIVEN DIRECT PHOTOSYNTHETIC PRODUCTION OF FUELS AND CHEMICALS - Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-l-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate. | 2016-01-28 |
20160024533 | ELECTRO-AUTOTROPHIC SYNTHESIS OF HIGHER ALCOHOLS - The disclosure provides a process that converts CO | 2016-01-28 |
20160024534 | Increased Heterologous Fe-S Enzyme Activity in Yeast - Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol. | 2016-01-28 |
20160024535 | ELECTROCHEMICAL DETOXIFICATION METHOD OF WOOD-BASED HYDROLYSATE FOR PRODUCING BIOCHEMICALS OR BIOFUELS, AND DETOXIFIED WOOD-BASED HYDROLYSATE - Provided is a method for producing a saccharified solution of wood-based biomass having toxicity reduced or removed, comprising the steps of: preparing a saccharified solution in which wood-based biomass has been pretreated by hydrolysis; and reducing toxicity in the saccharified solution using an electrochemical detoxification method. The method is capable of efficiently removing the toxicity of compounds which are produced during the pretreatment process and inhibit growth of microorganisms and fermentation. In addition, the method is capable of increasing the efficiency of production by minimizing sugar loss and additional costs during the toxicity removal process. | 2016-01-28 |
20160024536 | GENETICALLY ENGINEERED AND ACID-RESISTANT YEAST CELL WITH ENHANCED ERG5 ACTIVITY AND METHOD OF PRODUCING LACTATE BY USING THE YEAST CELL - Provided is a recombinant acid resistance yeast cell that is genetically engineered to increase ERG5 activity and a method of producing lactate by using the yeast cell. | 2016-01-28 |
20160024537 | GENETICALLY ENGINEERED AND STRESS RESISTANT YEAST CELL WITH ENHANCED MSN2 ACTIVITY AND METHOD OF PRODUCING LACTATE USING THE SAME - Provided is a yeast cell having a stress tolerance, wherein the yeast cell has enhanced MSN2 activity, a method of producing the yeast cell, and a method of producing lactate by using the same. | 2016-01-28 |
20160024538 | Hydroxylated Triacylglycerides - Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants. | 2016-01-28 |
20160024539 | YEAST HAVING IMPROVED PRODUCT PRODUCTIVITY AND METHOD OF PRODUCING PRODUCT - A genetically modified yeast cell that is capable of consuming glucose at an increased rate and a method of efficiently producing pyruvate or pyruvate-derived products by using the yeast cell. | 2016-01-28 |
20160024540 | Process and Intermediates for the Preparation of Pregabalin - The invention provides processes for the manufacture of a compound of formula (I | 2016-01-28 |
20160024541 | Compositions and Methods of Producing Methionine - Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed. | 2016-01-28 |
20160024542 | METHOD FOR PRODUCING BACTERIOCHLOROPHYLL A - A fed-batch fermentation method is for production of bacteriochlorophyll a (Bchla) from | 2016-01-28 |
20160024543 | Microorganisms and Methods for Producing Sialylated and N-Acetylglucosamine-Containing Oligosaccharides - The invention provides compositions and methods for engineering bacteria to produce sialylated and N-acetylglucosamine-containing oligosaccharides, and the use thereof in the prevention or treatment of infection. | 2016-01-28 |
20160024544 | MICROBIAL-DERIVED CHONDROITIN SULFATE - Described is chondroitin sulfate obtained from microbial sources, and related compositions and methods. | 2016-01-28 |
20160024545 | METHODS OF CONVERTING LIGNING AND USES THEREOF - Method for creating valuable products from lignocellulosic biomass comprising sequential pretreatment of lignocellulosic biomass with ionic liquid followed by hydrothermal processing of the lignin. | 2016-01-28 |
20160024546 | Methods for Amplification of Nucleic Acids Utilizing a Circularized Template Prepared from a Target Nucleic Acid - The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid. | 2016-01-28 |
20160024547 | MANUFACTURING METHODS FOR PRODUCTION OF RNA TRANSCRIPTS - Described are methods for production of RNA transcripts using a non-amplified, linearized DNA template in an in vitro transcription reaction. Enzymatic | 2016-01-28 |
20160024548 | DNA POLYMERASES AND RELATED METHODS - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects of a variety of polymerase and reverse transcriptase inhibitors. Therefore, the mutant polymerases are useful in a variety of disclosed methods in the presence of such inhibitors. | 2016-01-28 |
20160024549 | BACTERIAL DETECTION CARTRIDGE - Described herein are methods and apparatus for rapid detection of microorganisms in biological samples (e.g. blood) for analysis to determine the presence or absence of infectious microorganisms in the samples. The apparatus includes a cartridge with a lid and a tray, a mechanism for isolating a bulk sample into multiple smaller samples, and a sensor disposed on the tray to determine the presence or absence of microorganisms. The cartridge lid includes projections that, in a first position, allow for sample to distribute evenly in the cartridge tray and, in a second position, isolate the sample into multiple smaller volume samples. The apparatus and method shorten the time-to-detection of a microorganism in a sample and reduce the steps required from sample collection to microorganism detection. | 2016-01-28 |
20160024550 | BACTERIAL IDENTIFICATION - The present invention provides for separation of bacterial species and serotypes using electrophoretic methods. | 2016-01-28 |
20160024551 | MULTI-TIERED, HIGH THROUGH-PUT SCREEN FOR COMPOUNDS EFFECTIVE AGAINST BACTERIAL BIOFILM COMPOUNDS EFFECTIVE FOR INHIBITING AND ERADICATING BACTERIAL BIOFILM - A high through-put screening method for identifying agents effective for inhibiting biofilm formation and/or killing established biofilm are disclosed. The method includes three tiers, and each tier includes three specific biological process assays. The tier levels are a primary screen, a confirmation screen, and a dose-response screen, and the biological process assays include as says for total bacterial growth, bacterial metabolic activity, and biofilm formation. | 2016-01-28 |
20160024552 | BIOSENSING SYSTEM WITH EXTENDED LIFETIME VIA COFACTOR RECYCLING - The present disclosure relates to biosensing systems and biosensing elements having increased storage capability and increased functional lifetimes through using compositions and methods for recycling cofactors. | 2016-01-28 |
20160024553 | MEASUREMENT METHOD FOR HUMAN PANCREATIC LIPASE ACTIVITY - A measurement method for human pancreatic lipase activity in a sample, includes a contact step of bringing a bile acid that makes a pH for giving a maximum value of human pancreatic lipase activity to be lower than 7.7, a diglyceride and a colipase into contact with the sample at pH 7.4 or lower; and a detection step of detecting a signal amount varying in accordance with the human pancreatic lipase activity in the sample, and the bile acid is a bile acid containing: one of or two or more of a-type bile acids selected from the group consisting of GDCA, GCDCA, TDCA, TCDCA and salts thereof; and/or a combination of one of or two or more of b-1-type bile acids selected from the group consisting of GCA, GUDCA, TCA, TUDCA and salts thereof, and one of or two or more of b-2-type bile acids selected from the group consisting of DCA, CDCA and salts thereof. | 2016-01-28 |
20160024554 | RAPID, LOW-SAMPLE-VOLUME CHOLESTEROL AND TRIGLYCERIDE ASSAYS - Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures. | 2016-01-28 |
20160024555 | Method for Generating A Three-Dimensional Nucleic Acid Containing Matrix - Methods of making a three-dimensional matrix of nucleic acids within a cell is provided. | 2016-01-28 |
20160024556 | ENRICHMENT AND NEXT GENERATION SEQUENCING OF TOTAL NUCLEIC ACID COMPRISING BOTH GENOMIC DNA AND cDNA - The present invention relates to methods of enriching and sequencing a mixture of nucleic acids comprising genomic DNA sequences and cDNA sequences obtained from the test sample. | 2016-01-28 |
20160024557 | SIMULTANEOUS DETECTION OF TARGET PROTEIN AND TARGET NUCLEIC ACIDS IN A SINGLE CELL - Methods and reagents for detection and analysis of nucleic acids are provided. The methods employ proximity extension assays for detection of a target nucleic acids of interest, e.g., a target RNA. The method can additionally be used in multiplex assays with a protein proximity extension assay to detect protein. | 2016-01-28 |
20160024558 | NUCLEIC ACID BINDING PROTEINS AND USES THEREOF - Compositions, systems and methods employing nucleic acid binding proteins for use in the regulation and/or modulation of nucleic acid based reactions, including transcription, translation, modification, digestion, and hybridization reactions. Such compositions are employed in controlling a variety of different reaction types involving nucleic acids. | 2016-01-28 |
20160024559 | SPECIMEN COLLECTION WITH REAGENT LINED COLLECTORS - The present disclosure relates to specimen collection devices comprising stabilizing compositions for the collection, preservation, transport, and analysis of evidence samples for forensic analysis. | 2016-01-28 |
20160024560 | DETECTION OF NEISSERIA GONORRHOEAES - Methods and compositions for detection of | 2016-01-28 |
20160024561 | COMPOSITIONS AND METHODS FOR THE DETECTION AND ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS - Provided herein are compositions and methods useful for the detection of MTB. In particular, provided herein are kits, reagents, reaction mixtures, and methods involving such for nucleic acid amplification and detection procedures, which specifically and sensitively detect MTB in samples. | 2016-01-28 |
20160024562 | POLYNUCLEOTIDES FOR THE AMPLIFICATION AND DETECTION OF CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOEAE - Polynucleotides useful for detecting | 2016-01-28 |
20160024563 | METHOD FOR PERFORMING A MELTING CURVE ANALYSIS - The present invention related to methods for improving probe-based melting curve analysis methods. The improvement comprises the inclusion of a compound (A) which is a water-soluble polyanionic co-polymer comprising maleic acid, preferably poly(acrylic acid-co-maleic acid) (PAMA) in the analytical sample that is subjected to the melting curve analysis. | 2016-01-28 |
20160024564 | METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING - Disclosed herein are methods for determining the copy number of a chromosome in a fetus in the context of non-invasive prenatal diagnosis. In an embodiment, the measured genetic data from a sample of genetic material that contains both fetal DNA and maternal DNA is analyzed, along with the genetic data from the biological parents of the fetus, and the copy number of the chromosome of interest is determined. In an embodiment, the maternal serum is measured using a single-nucleotide polymorphism (SNP) microarray, along with parental genomic data, and the determination of the chromosome copy number is used to make clinical decisions pertaining to the fetus. | 2016-01-28 |
20160024565 | FRAGMENT COMPLEMENTATION OF BASED ASSAYS - The present disclosure provides, among other things, methods and compositions for detecting and/or quantifying analytes using fragment complementation technologies. In accordance with various embodiments of the present disclosure, a kit can include a) a capture probe immobilized on a surface, wherein the capture probe can associate with an analyte in a sample, thereby forming at least one captured analyte; b) a detection element including a target interacting probe associated with a first subunit of a detectable entity, wherein the target interacting probe can associate with the captured analyte so that a first complex is formed; and c) a second subunit that can complement the first subunit and generate a detectable entity, wherein the presence and/or amount of the analyte is indicated by detecting level or activity of the detectable entity. | 2016-01-28 |
20160024566 | COMPOSITIONS, METHODS, AND KITS FOR AMPLIFYING NUCLEIC ACIDS - The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided. | 2016-01-28 |
20160024567 | ELECTRONIC LABEL FREE DETECTION OF DNA COMPLEXES USING NANOGAP - A technique for electrical detection of a molecule is provided. A fluidic bridge is formed between a nanopipette and a fluid cell, where the molecule is in the nanopipette. A voltage difference is applied between the nanopipette and the fluid cell, where the fluid cell contains an electrolyte solution. Entry of the molecule into the fluidic bridge is determined by detecting a fore pulse. The fluidic bridge between the nanopipette and the fluid cell is broken to form a nanogap. In response to waiting a time interval, the fluidic bridge is reformed between the nanopipette and the fluid cell to close the nanogap. The molecule is determined to exit the nanopipette by detecting an after pulse. | 2016-01-28 |
20160024568 | COMPOSITIONS AND METHODS OF NUCLEIC ACID-TARGETING NUCLEIC ACIDS - This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof. Genome engineering can refer to altering the genome by deleting, inserting, mutating, or substituting specific nucleic acid sequences. The altering can be gene or location specific. Genome engineering can use nucleases to cut a nucleic acid thereby generating a site for the alteration. Engineering of non-genomic nucleic acid is also contemplated. | 2016-01-28 |
20160024569 | NANOFLUIDIC DEVICES FOR THE RAPID MAPPING OF WHOLE GENOMES AND RELATED SYSTEMS AND METHODS OF ANALYSIS - Devices and methods generate an ordered restriction map of genomic DNA extracted from whole cells, nuclei, whole chromosomes, or other sources of long DNA molecules. The devices have a fluidic microchannel that merges into a reaction nanochannel that merges into a detection nanochannel at an interface where the nanochannel diameter decreases in size by between 50% to 99%. Intact molecules of DNA are transported to the reaction nanochannel and then fragmented in the reaction nanochannel using restriction endonuclease enzymes. The reaction nanochannel is sized and configured so that the fragments stay in an original order until they are injected into the detection nanochannel. Signal at one or more locations along the detection nanochannel is detected to map fragments in the order they occur along a long DNA molecule. | 2016-01-28 |
20160024570 | RAMAN CLUSTER TAGGED MOLECULES FOR BIOLOGICAL IMAGING - This invention provides nucleoside polyphosphate analogs each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs; and methods for determining the sequence of a single-stranded DNA or RNA using said nucleoside polyphosphate analogs. This invention also provides methods for detecting the interaction of a plurality of predetermined compounds, at least one of which having attached thereto a tag comprising a plurality of Raman-scattering moieties. | 2016-01-28 |
20160024571 | Scalable Characterization of Nucleic Acids by Parallel Sequencing - A method for detecting the presence or absence of a target polynucleotide in a sample is described. | 2016-01-28 |
20160024572 | Massively Multiplexed RNA Sequencing - A method for parallel sequencing target RNA from samples from multiple sources while maintaining source identification is provided. The method includes providing samples of RNA comprising target RNA from two or more sources; labeling, at the 3′ end, the RNA from the two or more sources with a first nucleic acid adaptor that comprises a nucleic acid sequence that differentiates between the RNA from the two or more sources; reverse transcribing the two or more sources to create a single stranded DNA comprising the nucleic acid sequence that differentiates between the RNA from the two or more sources; amplifying the single stranded DNA to create DNA amplification products that comprise the nucleic acid sequence that differentiates between the RNA from the two or more sources; sequencing the DNA amplification products thereby parallel sequencing target RNA from samples from multiple sources while maintaining source identification. | 2016-01-28 |
20160024573 | PHOTOCLEAVABLE DEOXYNUCLEOTIDES WITH HIGH-RESOLUTION CONTROL OF DEPROTECTION KINETICS - Provided herein are new classes of photocleavable deoxynucleotides that allow for more precise control over deprotection kinetics compared to previously described compounds. The compounds further feature more favorable solubility properties. The nucleotides find use in methods such as next-generation sequencing. A series of molecules are provided with defined organic substituents that allow fine tuning of the deprotection kinetics when irradiated with an appropriate light source. | 2016-01-28 |
20160024574 | DNA SEQUENCING WITH NON-FLUORESCENT NUCLEOTIDE REVERSIBLE TERMINATORS AND CLEAVABLE LABEL MODIFIED NUCLEOTIDE TERMINATORS - This invention provides a process for sequencing nucleic acids using 3′ modified deoxynucleotide analogues or 3′ modified deoxyinosine triphosphate analogues, and 3′ modified dideoxynucleotide analogues having a detectable marker attached to a base thereof. | 2016-01-28 |
20160024575 | CIRCULATING SMALL NONCODING RNA MARKERS - This application describes small noncoding RNA markers that can be found in a biological sample taken from an individual. The level of such markers are useful for determining the individual's health status, especially in comparison with others. Methods and kits for the use of these markers are provided as well. | 2016-01-28 |
20160024576 | NUCLEIC ACID CONSTRUCTS AND METHODS OF USE - The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like. | 2016-01-28 |
20160024577 | Methods for Identifying Stem Cells Based on Nuclear Morphotypes - Methods for identifying stem cells and other cells specific to embryogenesis and carcinogenesis, classifying tissue samples, diagnosing precancerous and cancerous or atherosclerotic lesions, testing the value of anticancer agents, discovering macromolecules specifically expressed in particular cell types, using stem cells in restorative tissue therapy as well as methods for preparing tissue samples so heteromorphic nuclear morphotypes remain intact are disclosed. | 2016-01-28 |
20160024578 | METHOD FOR IDENTIFYING THE QUANTITATIVE CELLULAR COMPOSITION IN A BIOLOGICAL SAMPLE - The present invention provides an epigenetic haemogram, also referred to as an epigenetic blood cell count that identifies the quantitative, comprehensive picture of cellular composition in a biological sample, wherein advantageously a normalization standard is used. The normalization standard is a nucleic acid molecule comprising at least one marker-region being specific for each of the blood cells to be detected, and at least one control-region being cell-unspecific, wherein said regions are present in the same number of copies on said molecule and/or a natural blood cell sample of known composition. Furthermore, the present invention relates to a kit and the use of a kit for performing the epigenetic assessment of comprehensive, quantitative cellular composition of a biological sample. The biological sample is derived from e.g. a mammalian body fluid, including peripheral, capillary or venous blood samples or subfractions thereof, such as peripheral blood mononuclear cells or peripheral blood monocytes, or a tissue sample, organ sample, or from frozen, dried, embedded, stored or fresh body fluids or tissue samples. | 2016-01-28 |
20160024579 | NON-INVASIVE DETERMINATION OF FETAL INHERITANCE OF PARENTAL HAPLOTYPES AT THE GENOME-WIDE SCALE - The present invention provides a method, device and a computer program for haplotyping single cells, such that a sample taken from a pregnant female, without directly sampling the fetus, provides the ability to non-invasively determine the fetal genome. The method can be performed by determining the parental and inherited haplotypes, or can be performed merely on the basis of the mother's genetic information, obtained preferably in a blood or serum sample. The novel device allows for sequence analysis of single chromosomes from a single cell, preferably by partitioning single chromosomes from a metaphase cell into long, thin channels where a sequence analysis can be performed. | 2016-01-28 |
20160024580 | POLYMORPHISM OF THROMBOSPONDIN-1 AS A BIOMARKER FOR SUSCEPTABILTIY TO DRY EYE - Embodiments of the invention relate to single nucleotide polymorphisms (SNPs) in Thrombospondin 1 gene (THB-S1) that are herein linked to an increased risk for dry eye (also known as keratoconjunctivitis). The polymorphisms disclosed herein are directly useful for the diagnosis of an increased risk for dry eye, as well as for the determination of patient treatment pre- and post-surgery (e.g. pre and post refractive eye surgery) or in a patient having a condition associate with dry eye (e.g. diabetes, lupus, scleroderma, Sjogren's syndrome, thyroid disorders, vitamin A deficiency, and rheumatoid arthritis). Computer systems and medium are also enclosed. The SNPs can be present in unique combinations, or as specific haplotypes indicative of dry eye. The SNPs linked to an increased risk for dry eye include rs1478604, rs2228261, and rs2292305. | 2016-01-28 |
20160024581 | METHODS AND COMPOSITIONS FOR ASSESSING RENAL STATUS USING URINE CELL FREE DNA - The present invention relates to non-invasive tools and methods for evaluating renal status and renal health using urine cell free DNA. | 2016-01-28 |
20160024582 | MICROARRAY FOR EVALUATING EYE DISEASE, AND EVALUATION METHOD OF EYE DISEASE - The purpose of the present invention is to provide a method for objectively evaluating the state of eye disease in a test organism. Provided is a microarray for evaluating the state of eye disease. Further, this method for evaluating the state of eye disease in a test organism is characterized by detecting, from a sample taken from the test organism, at least one gene from a prescribed gene group and comparing the obtained detection result with a control. | 2016-01-28 |
20160024583 | METHODS FOR EVALUATING COPD STATUS - The invention in some aspects provides methods of determining the likelihood that a subject has COPD based on the expression of informative-genes. In other aspects, the invention provides methods for determining an appropriate diagnostic intervention plan for a subject based on the expression of informative-genes. Related compositions and kits are provided in other aspects of the invention. | 2016-01-28 |
20160024584 | METHODS AND SYSTEMS FOR INFERRING BOVINE TRAITS - Methods, compositions, and systems are provided for managing bovine subjects in order to maximize their individual potential performance and edible meat value, and to maximize profits obtained in marketing the bovine subjects. The methods and systems draw an inference of a trait of a bovine subject by determining the nucleotide occurrence of at least one bovine SNP that is identified herein as being associated with the trait. The inference is used in methods of the present invention to establish the economic value of a bovine subject, to improve profits related to selling beef from a bovine subject; to manage bovine subjects, to sort bovine subjects; to improve the genetics of a bovine population by selecting and breeding of bovine subjects, to clone a bovine subject with a specific trait, to track meat or another commercial product of a bovine subject; and to diagnose a health condition of a bovine subject. Methods are also disclosed for identifying additional SNPs associated with a trait, by using the associated SNPs identified herein. | 2016-01-28 |
20160024585 | METHODS OF PREDICTING RESPONSIVENESS OF A CANCER TO AN AGENT AND METHODS OF DETERMINING A PROGNOSIS FOR A CANCER PATIENT - The present disclosure provides biomarkers, and methods of using such biomarkers, that are predictive for efficacy of and resistance to an EGFR targeting agent, such as cetuximab, or prognostic with respect to cancer survival. | 2016-01-28 |
20160024586 | METHODS OF DETECTING CANCER - Methods of detecting cancer are provided. Methods of detecting changes in the levels of one or more small RNAs associated with cancer are also provided. Compositions and kits are also provided. | 2016-01-28 |
20160024587 | MARKERS ASSOCIATED WITH WNT INHIBITORS - The invention provides methods of monitoring differential gene expression of biomarkers to determine patient sensitivity to Wnt inhibitor, methods of determining the sensitivity of a cell to an Wnt inhibitor by measuring biomarkers, methods of screening for candidate Wnt inhibitor, Wnt inhibitor for use in head and neck squamous cell carcinoma. | 2016-01-28 |
20160024588 | OSTEOSARCOMA-ASSOCIATED RISK MARKERS AND USES THEREOF - Provided herein are methods and compositions for identifying subjects, including canine subjects, as having an elevated risk of developing cancer or having an undiagnosed osteosarcoma. These subjects are identified based on the presence of germ-line risk markers. | 2016-01-28 |
20160024589 | METHODS OF IDENTIFYING RESPONSES TO MAP KINASE INHIBITION THERAPY - The invention provides methods and devices for determining molecular signatures in a cancer that predict response to a MARPK pathway inhibitor and methods of use of such signatures. | 2016-01-28 |
20160024590 | FALZ FOR USE AS A TARGET FOR THERAPIES TO TREAT CANCER - The disclosure provides methods for predicting and/or determining whether a subject has cancer based on the level of expression of BPTF. The disclosure also provides methods for determining whether a cancer in a subject is progressing or regressing based upon the change of expression levels of BPTF between two time points. The disclosure further provides methods to treat a subject with a cancer by administering a polynucleotide comprising an inhibitory BPTF nucleic acid and/or an agent that inhibits the expression or activity of BPTF. | 2016-01-28 |
20160024591 | METHODS AND COMPOSITIONS FOR CORRELATING GENETIC MARKERS WITH RISK OF AGGRESSIVE PROSTATE CANCER - The present invention provides a method of identifying a subject as having an increased risk of having or developing aggressive prostate cancer, comprising detecting in the subject the presence of various genetic markers associated with an increased risk of having or developing aggressive prostate cancer. | 2016-01-28 |
20160024592 | SINGLE-CELL ANALYSIS AS A SENSITIVE AND SPECIFIC METHOD FOR EARLY PROSTATE CANCER DETECTION - Certain embodiments are directed to methods of measuring single cell levels of biomarkers associated with prostate cancer. | 2016-01-28 |
20160024593 | METHODS AND COMPOSITIONS RELATED TO T-CELL ACTIVITY - Embodiments concern methods and composition related to anergic T-cells in patients, such as cancer patients. T cell anergy is a hyporesponsive state induced by TCR engagement in the absence of costimulation (Schwartz, 2003). Anergy induction was initially observed in vitro using chemically-fixed antigen presenting cells (APCs). Subsequently, it was found that anergy could be induced by immobilized anti-CD3 mAb or calcium ionophores (such as ionomycin) in vitro, and by superantigen and soluble antigenic peptide in vivo. Indirect evidence has suggested that T cell dysfunction in the tumor microenvironment and establishment of transplant tolerance is partially due to T cell anergy (Gajewski et al., 2011). | 2016-01-28 |
20160024594 | BIOMARKERS FOR RADIATION TREATMENT - The methods described herein allow for the classification of patients into groups for receiving optimized radiation treatment based on patient specific biomarker signature. The biomarker signature includes markers that have been shown to correlate with TGF-β expression and to be associated with tumor aggressiveness, radioresistance and poor prognosis. The markers play a key role in the epithelial-mesenchymal transition. The methods described herein provide the dual benefits of anti-tumor efficacy plus normal tissue protection when combining TGF-β inhibitors with ionizing radiation to treat cancer patients. | 2016-01-28 |
20160024595 | CHARACTERIZATION OF MELANOMA USING A MOLECULAR SIGNATURE - The present disclosure provides methods for characterizing melanoma in a subject by analyzing genes or gene expression products obtained from the subject. The present disclosure provides methods for distinguishing melanoma in situ from invasive melanoma. The disclosure includes non-invasive methods for obtaining genes or gene expression products from a pigmented skin lesion of a subject. | 2016-01-28 |
20160024596 | Prognostic and Predictive Gene Signature for Non-Small Cell Lung Cancer and Adjuvant Chemotherapy - The application provides methods of prognosing and classifying lung cancer patients into poor survival groups or good survival groups and for determining the benefit of adjuvant chemotherapy by way of a multigene signature. The application also includes kits and computer products for use in the methods of the application. | 2016-01-28 |
20160024597 | miRNAs AS THERAPEUTIC TARGETS IN CANCER - Methods for modulating expression of a component of a cell, comprising contacting the cell with a nucleic acid comprising an miR-140 nucleic acid sequence in an amount sufficient to modulate the cellular component are provided. Overexpression of miR-140 inhibits cell proliferation in both U-2 OS (wt-p53) and HCT 116 (wt-p53) cell lines. Cells transfected with miR-140 are more resistant to chemotherapeutic agent methotrexate. mi-140 expression is related to HDAC4 protein expression. The claimed methods reduce the protein expression level of HDAC4 without degrading the target mRNA. | 2016-01-28 |
20160024598 | COMPOSITIONS AND METHODS TO SELECT FOR REDUCED GRAIN MOISTURE IN MAIZE - The invention relates to methods and compositions for identifying and selecting maize plants that have reduced grain moisture. Maize plants generated by the methods of the invention are also a feature of the invention. | 2016-01-28 |
20160024599 | COMPOSITIONS ASSOCIATED WITH SOYBEAN REPRODUCTIVE GROWTH AND METHODS OF USE - Molecular markers associated with soybean reproductive stage, methods of their use, and compositions having one or more marker loci are provided. Methods comprise detecting at least one marker locus, detecting a haplotype, and/or detecting a marker profile. Methods may further comprise crossing a selected soybean plant with a second soybean plant. Isolated polynucleotides, primers, probes, kits, systems, etc., are also provided. | 2016-01-28 |
20160024600 | COINCIDENCE REPORTER GENE SYSTEM - Disclosed is a nucleic acid comprising a nucleotide sequence encoding (i) two or more reporters comprising a first reporter and a second reporter that is different from the first reporter; and (ii) one or more ribosomal skip sequences, wherein a ribosomal skip sequence is positioned between the first and second reporters, wherein the first and second reporters are stoichiometrically co-expressed from the nucleotide sequence and the nucleic acid does not comprise a cytomegalovirus-immediate early (CMV-IE) promoter. Also disclosed are methods of screening test compounds for ability to modulate a biological activity of interest using the nucleic acid, as well as related recombinant expression vectors, host cells, and populations of cells. | 2016-01-28 |
20160024601 | HIV VIRAL LOAD TESTING - Methods of testing HIV viral load are described. The methods comprise detecting HIV viral RNA in a sample of leukocyte-depleted blood. Such methods can be carried out on low-volume samples obtained without the need for venipuncture or a centrifuge. The methods are particularly suited for HIV viral load testing in resource-limited settings. Methods for monitoring HIV infection are also described, as well as kits for carrying out the methods. | 2016-01-28 |
20160024602 | Methods of Evaluating a Cellular Sample for Latent Cellular Replication Competent HIV-1, and Compositions and Kits for Use in Practicing the Same - Methods of evaluating a cellular sample for latent cellular replication competent HIV-1 are provided. Aspects of the methods include contacting a cellular sample with an HIV-1 inducing compound to produce an activated cellular sample; and assessing plasma viral load in the activated cellular sample to evaluate the cellular sample for latent cellular replication competent HIV-1. Also provided are devices and kits that find use in practicing the methods. | 2016-01-28 |
20160024603 | NOVEL EXTREME THERMOPHILIC BACTERIA OF THE GENUS CALDICELLULOSIRUPTOR - A method for converting lignocellulosic biomass material to a carboxylic acid comprising the step of contacting the lignocellulosic biomass material with a microbial culture for a period of time at an initial temperature and an initial pH, thereby producing an amount of a carboxylic acid; wherein the microbial culture comprises an extremely thermophilic bacteria strain selected from the group consisting of | 2016-01-28 |
20160024604 | METHOD FOR HEATING A BLAST FURNACE STOVE - A method for heating a blast furnace stove having a combustion chamber with a combustion zone therein, includes causing combustion gases to flow through and be exhausted from refractory material in the stove for heating said material, supplying and adding an oxidant comprising an oxygen content of at least 85% into the combustion chamber for recirculating the combustion gases including said oxidant into the combustion zone, wherein the recirculating the combustion gases is from a location inside the combustion chamber, but outside an area of the combustion chamber occupied by the combustion zone, and the supplying the oxidant to the combustion zone is at high velocity through at least one lance, thereby entraining the combustion gases into the combustion zone, diluting a mixture of the fuel and the oxidant with the recirculated combustion gases, and flamelessly combusting said mixture in the combination zone. | 2016-01-28 |
20160024605 | Method for Producing a Profile and a Manufacturing System for Producing a Profile - A method for producing a profile includes method steps of: providing a workpiece; shaping the workpiece; joining the workpiece; coating the workpiece; heating the workpiece; and at least partially hardening the workpiece; wherein the coating method step is carried out temporally after the joining method step and temporally before the heating method step. | 2016-01-28 |
20160024606 | SUB-ZERO TREATMENT DEVICE - The present invention provides a sub-zero treatment device capable of uniformly cooling a cooling target object and reducing the amount of liquid refrigerant used for cooling the cooling target object. The sub-zero treatment device has an exhaust member extending from a through-hole provided in a cooling tank constituting a cooling target object mounting chamber through to the interior of the cooling target object mounting chamber, and having an exhaust port, wherein the exhaust port is disposed in an exhaust port positioning space, which is the space located in the upper half of the cooling target object mounting chamber and having a width in the transverse direction that is equal to the maximum width in the transverse direction of the suction port. | 2016-01-28 |
20160024607 | System and Method for Producing a Hardened and Tempered Structural Member - System and methods relating to in-line heat-treating, hardening and tempering of material, such as for example, coiled steel into a roll-formed, hardened and tempered structural member having uniform or different targeted properties in selected zones of the structural member. The different targeted properties may be achieved by heating and/or cooling the material subject to certain parameters. | 2016-01-28 |
20160024608 | PROCESSING OF HOT STAMPED PARTS - A method of manufacturing a steel part including hot stamping followed by trimming, piercing, or flanging, without delayed fracture and without the need for annealing, is provided. The method includes heating a blank formed of a steel material, forming the blank between a pair of dies, and quenching the blank. The temperature drop in select areas of the blank is reduced, which limits the amount of martensite formed in the select areas, but allows martensite to form in other areas. The dies can be formed with modified materials or modified cooling channels to limit the amount of martensite formed in the select areas of the blank. The select areas are softer than the other areas and can be subsequently trimmed, pierced, or flanged without the delayed fractures. | 2016-01-28 |
20160024609 | ROLLER HEARTH FURNACE AND METHOD FOR THE HEAT TREATMENT OF METAL SHEETS - The invention relates to a roller hearth furnace for the heat treatment of coated metal sheets as well as to a corresponding method. The objective of the invention is to put forward a roller hearth furnace that allows the alternating processing of AlSi-coated metal sheets and metal sheets with zinc alloy coatings for hot-working, whereby considerably less effort is involved for the alternating procedure than is the case in the state of the art. The roller hearth furnace according to the invention for the heat treatment of coated metal parts is characterized in that it has at least a first zone and a second zone, whereby in the first zone, a temperature below the melting temperature of the AlSi deposits or a temperature of more than approximately 900° C. [1652° F.] can be maintained, whereas in the second zone, a temperature of more than approximately 870° C. [1598° F.] can be reached. | 2016-01-28 |
20160024610 | HIGH STRENGTH STEEL SHEET EXCELLENT IN DELAYED FRACTURE RESISTANCE AND LOW TEMPERATURE TOUGHNESS, AND HIGH STRENGTH MEMBER MANUFACTURED USING THE SAME - A high strength steel sheet containing chemical components of, in mass %, C: 0.20 to 0.42%, Si: 0.06 to 0.5%, Mn: 0.2 to 2.2%, Cr: 0.1 to 2.5%, B: 0.0005 to 0.01%, O: 0.0020 to 0.020%, Al: 0.001 to 0.03%, Ti: 0.001 to 0.05%, N: 0.1% or less, P: 0.03% or less, S: 0.02% or less, and the balance: Fe and inevitable impurities. In steel, 5×10 | 2016-01-28 |
20160024611 | METHOD FOR RECYCLING IRON-CONTAINING BY-PRODUCTS DISCHARGED FROM COAL-BASED MOLTEN IRONMAKING PROCESS, SYSTEM THEREFOR, AND REDUCED IRON AGGLOMERATION SYSTEM - There is provided a method and system for recycling by-products containing large amounts of useful components and discharged in the form of dust and sludge from a coal-based molten iron making process to reuse the by-products in a reduced iron agglomeration process. The system includes: a fluidized reduction furnace reducing fine iron ore; a reduced iron tank connected to the fluidized reduction furnace through a reduced iron discharge pipe for storing the reduced iron and supplying the reduced iron in an agglomeration system; an agglomeration system agglomerating the reduced iron transferred from the reduced iron tank; and a transfer unit transferring compactions of by-products discharged from a molten iron making process through a by-product supply pipe. The compactions of the by-products are supplied to at least one selected from the group consisting of the fluidized reduction furnace, the reduced iron supply pipe, and the reduced iron tank. | 2016-01-28 |
20160024612 | Method And Device For Obtaining Pure, Additive-Free Scrap Iron From A Mixture Of Comminuted Scrap Metal - A method and a device for obtaining scrap iron from a mixture of comminuted scrap metal. The device and the method are characterized in that individual steps are carried out in order to remove iron from additives adhering to the iron. Emphasis is placed on copper and copper-containing additives. Comminuted scrap metal is divided into small and large scrap parts using a sieve device. The small scrap parts are transported to a scrap sorting machine, and the large material parts are returned to the macerator. In the scrap sorting machine, copper-containing material is sorted out, and iron which still contains copper is guided to a special over-belt magnet via a vibrating trough, the over-belt magnet shaking and rocking the copper-containing material over the belt section such that nonmagnetic additives drop down from the iron, and iron with a copper content of only 0.01% to a maximum of 0.1% is obtained. | 2016-01-28 |
20160024613 | Use of Cationic Surfactants In the Cyanidation of Refractory Carbonaceous Ores for Recovery of Metals - A process for recovery of precious metals from ores or concentrates containing refractory carbonaceous material by cyanidation leaching. The process involves addition to the ores or concentrates at least one cationic surfactant before or during the addition of cyanide-containing solution. The agent enables the recovery of precious metals by cyanidation from high preg-robbing carbonaceous ores and improves the recovery of precious metals by cyanidation from medium to low preg-robbing carbonaceous ores. The agent also prevents froth and foaming formation during the cyanidation process. | 2016-01-28 |
20160024614 | HYDROMETALLURGICAL PLANT FOR NICKEL OXIDE ORE AND METHOD FOR OPERATING THE HYDROMETALLURGICAL PLANT - A hydrometallurgical plant according to the invention includes: a leaching facility provided with leaching treatment tanks in a plurality of lines to perform a leaching treatment; a preliminary neutralization facility provided with neutralization treatment tanks in two stages to perform pH adjustment of leach slurry; and solid-liquid separation facility made up of a single line to perform solid-liquid separation of leach slurry which is pH-adjusted and discharged from the preliminary neutralization facility, in which the preliminary neutralization facility is provided with neutralization treatment tanks of a first stage in a plurality of lines so as to correspond to the respective leaching treatment tanks, and is configured such that leach slurries which are pH-adjusted in the neutralization treatment tanks of the first stage are merged in a neutralization treatment tank | 2016-01-28 |
20160024615 | SODIUM TUNGSTATE PRODUCTION METHOD - A method of producing sodium tungstate of this invention including allowing cemented carbide scrap containing tungsten to react with a molten salt containing sodium sulfate and having added thereto a metal oxide, to thereby obtain sodium tungstate. | 2016-01-28 |
20160024616 | A method for re-extraction of rare-earth metals from organic solutions and preparing concentrate of rare-earth metals - The present invention relates to a method for recovery of rare earth metals (REM) from diluted aqueous acidic solutions comprising steps of liquid-phase extraction of REM into an organic phase and re-extraction of REM from the organic phase. The re-extraction is carried out by precipitation of REM as a solid phase in the form of a poorly soluble salt of a strong acid (pK | 2016-01-28 |
20160024617 | METHODS FOR GAS-PHASE THERMOCHROMATOGRAPHIC SEPARATIONS OF FISSION AND ACTIVATION PRODUCTS - Methods are provided for characterizing samples containing chemical elements such as rare earth elements, actinides, and heavy transition metals by treating the samples to form volatile complexes of the elements (e.g., β-diketonate complexes or other organic ligand complexes of the elements) and then analyzing the complexes, for example, via gas-phase thermochromatography. Also provided are methods for separating and/or recovering such chemical elements. The methods produce less waste and can be performed more rapidly than conventional liquid extraction methods and can provide separated elements of high purity (e.g., 99.9999% purity). | 2016-01-28 |
20160024618 | SHAPED METAL BODY AND METHOD FOR PRODUCING A SHAPED METAL BODY - Method for manufacturing a multi-layered shaped metal body includes introducing gas into a molten metal in order to form a flowable metal foam, introducing the flowable metal foam into a mold containing therein at least one insert element comprising a freely shearing chain mail of loosely interlinked rings, and cooling the flowable metal foam in the mold to form a shaped metal body. | 2016-01-28 |
20160024619 | CORRODIBLE DOWNHOLE ARTICLE - A magnesium alloy suitable for use as a corrodible downhole article. The alloy has a corrosion rate of at least 50 mg/cm | 2016-01-28 |