04th week of 2012 patent applcation highlights part 42 |
Patent application number | Title | Published |
20120021446 | Evaluation Peptide For Use In Quantification Of Protein Using Mass Spectrometer, Artificial Standard Protein, And Method For Quantifying Protein - Disclosed is an evaluation peptide for evaluating the efficiency of a pretreatment in the quantification of a protein using a mass spectrometer, having high reliability and high general versatility. Also disclosed is an artificial standard protein comprising the evaluation peptide. Further disclosed is a method for quantifying a protein utilizing the artificial standard protein. Specifically disclosed is a method for selecting a peptide which consists of an amino acid sequence not agreeing with that in a naturally occurring protein and a variant thereof and capable of being detected by mass spectrometry and which has an amino acid that can be recognized by a protein-digesting enzyme, and using the peptide as an evaluation peptide for use in the quantification of a protein by a mass spectrometer. | 2012-01-26 |
20120021447 | ANALYTICAL ROTORS AND METHODS FOR ANALYSIS OF BIOLOGICAL FLUIDS - Devices for generating discrete flow of liquids in response to a driving force, for example centrifugal microfluidic devices for generating discrete flow in response to a constant driving force. The device includes a supply structure for supplying liquid at an inflow rate to a discretization structure in response to a driving force. The discretization structure is shaped to define an outlet and a level to which the discretization structure fills with liquid flowing from the supply structure before dispensing the liquid at an outflow rate through the outlet in response to the driving force. The device is arranged such that the outflow rate from the discretization structure is greater than the inflow rate into the discretization structure, thereby periodically emptying the discretization structure to create a discretized flow from the outlet. The devices find applications in liquid mixing, for example for diluting samples, such as blood plasma samples. | 2012-01-26 |
20120021448 | METHOD FOR EVALUATING STATE OF CELLS - Provided are a novel marker of the state of cells and the differentiation level of the same which is usable as a substitute for a gene or a protein; a means for quantitatively understanding this marker; and a means for understanding the state of specific cells and the differentiation level of the same using this marker. A method for determining a sugar chain category to be used for evaluating the state of cells. The quantitative profile of N-linked sugar chains of cells before a change in the state thereof is obtained. Next, the quantitative profile of N-linked sugar chains contained in the cells after the change in the state thereof is obtained. Sugar chains showing variations in the contents thereof between these two quantitative profiles are extracted and the individual sugar chains contained in the extracted sugar chains are classified into the following categories based on sugar chain type: either the high-mannose type or the non-high-mannose type; (1) the presence/absence of a bisect; (2) the number fucose residues (0, 1, or 2 or more); (3) the presence/absence of sialic acid; and (4) the degree of branching (2 or 3 or more). From these five categories, a sugar chain category appropriate for evaluating the state of cells is determined. A method for evaluating the state of cells and the differentiation of cells using the thus determined sugar chain category. | 2012-01-26 |
20120021449 | DEVICE FOR AUTOMATICALLY ANALYZING MICROORGANISMS AND METHOD FOR AUTOMATICALLY ANALYZING MICROORGANISMS - After culturing blood, a culture liquid determined as positive is transplanted into a plate medium and a bacterial cell suspension that is directly usable for identifying and testing antibiotics-sensitivity is prepared without forming colonies. Provided are a device for automatically analyzing microorganisms and a method therefor whereby blood culture and an identification and antibiotics-sensitivity test can be continuously operated. A device for automatically analyzing microorganisms which is configured so that a blood culture test and an identification and antibiotics-sensitivity test can be automatically and continuously conducted, wherein means for pretreating a cultured blood sample comprises a mechanism for removing culture liquid components in the course of the blood culture and a mechanism for controlling the microbial concentration (bacterial cell count) to a constant level after the blood culture. | 2012-01-26 |
20120021450 | METHODS FOR MAGNETIC RESONANCE IMAGING - In certain aspects the present invention provides methods and compositions related to contrast agents for magnetic resonance imaging. In certain variations, contrast agents provided herein are generated in situ via genetic instructions and become potent upon sequestering available metal atoms. Exemplary contrast agents include metal-binding proteins. | 2012-01-26 |
20120021451 | SAMPLE ANALYSIS APPARATUS AND SAMPLE ANALYSIS METHOD - An analysis apparatus includes a plurality of separation channels, a detector, and a control unit. In each separation channel, a particular component contained in a sample is separated from other components. The detector detects the separated particular component. The control unit controls an analysis step and a preprocessing step. The analysis step includes a separation step of performing the separation and a detection step of performing the detection in each separation channel. The preprocessing step is performed to put each separation channel into a state in which it can perform the analysis step. The control unit simultaneously carries out at least parts of the preprocessing steps of at least two of the plurality of separation channels. | 2012-01-26 |
20120021452 | Belt Agitation System For Culture Bottles - A specimen culture container agitation assembly includes an endless belt having a multitude of container-receiving clips attached thereto, each of which is adapted for retaining a specimen culture container to the belt. The belt is oriented vertically. The assembly further includes upper and lower pulleys over which the belt is driven. A drive motor drives the belt around the pulleys. Agitation of the specimen culture container is achieved by rotation of the belt over the pulleys and the retaining of the specimen culture container by the clip. | 2012-01-26 |
20120021453 | LABEL-FREE CELL SORTING USING NEAR INFRARED EMISSION - Disclosed are methods and systems for identifying and sorting cells based on a near-infrared emission pattern of the cell in response to excitation at 630±nm. The NIR emission pattern can be used for monitoring and sorting of cells in a label-free manner, and thus provides a positive method for selecting cells, such as stem cells, for use in therapy. | 2012-01-26 |
20120021454 | RAPID FRET-BASED DIAGNOSIS OF BACTERIAL PATHOGENS - The invention comprises a substrate for detection of micro-organisms, wherein said substrate comprises a set of molecular markers linked, optionally with linker molecules or moeieties, to a di-, or tripeptide consisting of amino acids X1 and X2, or X1, X2 and X3, in which one of them, for example X1, is a D-amino acid and the others, for example X2 and X3, may be any D- or L-amino acid. Said substrate preferably is used for the detection of | 2012-01-26 |
20120021455 | Detector Arrangement for Blood Culture Bottles With Colorimetric Sensors - A detector arrangement for a blood culture bottle incorporating a colorimetric sensor which is subject to change of color due to change in pH or CO | 2012-01-26 |
20120021456 | METHOD AND APPARATUS FOR DETECTING THE PRESENCE OF ANISOTROPIC CRYSTALS AND HEMOZOIN PRODUCING PARASITES IN LIQUID BLOOD - An apparatus and method for detecting the presence of anisotropic crystals within a biologic fluid sample is provided. The method includes the steps of: a) disposing the sample within a sample chamber in a sample layer having a height riot greater than about fifteen microns (15μ); b) disposing the sample layer within the sample chamber between a polarizing filter and an analyzing filter; a) disposing the polarizing filter, sample chamber, and analyzing filter in a configuration relative to a light source such that polarized light passes through the sample, and subsequently impinges on the analyzing filter; and d) wherein polar orientations of the polarizing filter and the analyzing filter are such that the polarized light will not pass through the analyzing filter, and light passing through an anisotropic crystal disposed within the sample will pass through the analyzing filter and appear as a point of light. | 2012-01-26 |
20120021457 | PROTEIN CONCENTRATES AND ISOLATES, AND PROCESSES FOR THE PRODUCTION THEREOF FROM MACROALGAE AND/OR MICROALGAE - Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to the removal of fiber from macroalgae and/or microalgae using low g-force centrifugation. | 2012-01-26 |
20120021458 | THERMOTOLERANT TRANSGLUTAMINASE ORIGINATING IN ACTINOMYCES - The present invention provides transglutaminases with improved heat resistance. Specifically, the present invention provides mutant transglutaminase proteins with improved heat resistance as obtained by introducing appropriate mutations into transglutaminases, which results in the incorporation of a disulfide bond. | 2012-01-26 |
20120021459 | FEED MEDIA - The invention provides stable feed media containing pyruvate and methods for stabilizing feed media by adding pyruvate. The invention further provides methods for producing proteins using such media and proteins produced through the use of such methods. | 2012-01-26 |
20120021460 | MATERIALS AND METHODS FOR ISOTHERMAL NUCLEIC ACID AMPLIFICATION - A method for isothermal amplification of a target nucleic acid sequence is disclosed. The target nucleic acid is amplified by an enzyme with helicase activity and an enzyme with reverse transcriptase activity and DNA-dependant DNA polymerase activity. Also disclosed is a kit for isothermal amplification of a target nucleic acid sequence, including HPV nucleic acids. The kit comprises a first enzyme with helicase activity and a second enzyme having both reverse transcriptase activity and DNA-dependant DNA polymerase activity. | 2012-01-26 |
20120021461 | ISOTHERMAL STRAND DISPLACEMENT AMPLIFICATION - A method for isothermal DNA amplification comprising: providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing Xanthosine, a second primer at least partially complementary to a region of DNA and containing Xanthosine, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises Xanthosine in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near Xanthosine; and amplifying the DNA substantially without thermal cycling. | 2012-01-26 |
20120021462 | RECOMBINASE POLYMERASE AMPLIFICATION - This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods allow amplification of DNA up to hundreds of megabases in length. | 2012-01-26 |
20120021463 | METHOD AND APPARATUS FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES BY USING THERMAL CONVECTION - The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region. | 2012-01-26 |
20120021464 | THERMOSTABLE REVERSE TRANSCRIPTASES AND USES THEREOF - The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions. | 2012-01-26 |
20120021465 | RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 2012-01-26 |
20120021466 | Microorganisms of Corynebacterium with Improved 5'-Inosinic Acid Productivity, and Method for Producing Nucleic Acids Using Same - The present invention relates to a microorganisms of the genus | 2012-01-26 |
20120021467 | METHOD OF PRODUCING XYLITOL AND ARABINOSE AT SAME TIME FROM HEMICELLULOSE HYDROLYSATES - The present invention relates to a method of producing xylitol and arabinose at same time from hemicellulose hydrolysates, material rich in xylan is hydrolyzed by acid or enzymes to obtain hydrolysate mostly containing xylose and arabinose, then inoculated in | 2012-01-26 |
20120021468 | PRODUCTION OF L-RIBOSE AND OTHER RARE SUGARS - The invention provides methods and compositions for the production of L-ribitol and other rare sugars using a mannitol-1-dehydrogenase or a polyol-1-dehydrogenase. | 2012-01-26 |
20120021469 | AMIDASES AND METHODS OF THEIR USE - The disclosure relates to engineered amidase polypeptides and processes of using the polypeptides for chiral resolution of amino acid amide compounds. The disclosure further relates to the polynucleotides that encode the engineered amidase polypeptides and related vectors, host cells, and methods for making the engineered amidase polypeptides. | 2012-01-26 |
20120021470 | Chimeric PUFA Polyketide Synthase Systems and Uses Thereof - Disclosed are chimeric polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from | 2012-01-26 |
20120021471 | BIOABSORBABLE POLYMER CONTAINING 2-HYDROXYACID MONOMERS - Genetically engineered organisms for production of PHA copolymers containing 2-hydroxyacid monomers and the methods of making and using thereof have been developed. The copolymers containing 2-hydroxyacid monomers can be synthesized via biosynthesis by the action of a PHA polymerase in a living cell. By changing the genetic background of the cells, one can control specific metabolic pathways allowing control of the level of glycolic acid co-monomer in the PHA polymer. | 2012-01-26 |
20120021472 | Method for the Production of Glutaconate - The present invention relates to a novel method for the biocatalytic production of unsaturated dicarboxylic acids by cultivating a recombinant microorganism co-expressing a glutaconate CoA-transferase and a 2-hydroxyglutaryl-CoA dehydratase system. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as a method of preparing polyamide or polyester copolymers making use of said dicarboxylic acids as obtained by said biocatalytic production method. | 2012-01-26 |
20120021473 | PROCESSES FOR PRODUCING CARBOXYLIC ACIDS FROM FERMENTATION BROTHS CONTAINING THEIR AMMONIUM SALTS - Processes for making SA from either a clarified DAS-containing fermentation broth or a clarified MAS-containing fermentation broth that include distilling the broth under super atmospheric pressure at a temperature of >100° C. to about 300° C. to form an overhead that comprises water and ammonia, and a liquid bottoms that includes SA, and at least about 20 wt % water; cooling the bottoms to a temperature sufficient to cause the bottoms to separate into a liquid portion and a solid portion that is substantially pure SA; and separating the solid portion from the liquid portion. A method also reduces the broth distillation temperature and pressure by adding an ammonia separating and/or water azeotroping solvent to the broth. | 2012-01-26 |
20120021474 | BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID - The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production. | 2012-01-26 |
20120021475 | METHOD FOR PRODUCING (+)-ZIZAENE - A method of producing (+)-zizaene by contacting at least one polypeptide with farnesyl pyrophosphate (FPP) in vitro or in vivo to produce (+)-zizaene, a compound which can be used as precursor for diverse compounds useful in the fields of perfumery and flavoring. An amino acid sequence of a polypeptide useful in the method, a nucleic acid encoding the polypeptide of the invention, an expression vector containing the nucleic acid and a non-human host organism or a cell transformed to be used in the method of producing (+)-zizaene are also disclosed. | 2012-01-26 |
20120021476 | Trace Element Solution For Biogas Methods - The invention relates to a trace element solution for the supplementing of nutrients for an anaerobic fermentation, in particular a biogas process, comprising at least one trace element and at least two complexing agents. Complexing agents are used which (1) are able to transport the trace elements in complexed form across the cell membrane and which (2) release the trace elements in the cell. Where applicable, the complexing agents are biologically decomposable. | 2012-01-26 |
20120021477 | Method for the Fixation of CO2 and for Treating Organic Waste by Coupling an Anaerobic Digestion System and a Phytoplankton Microorganism Production System - The invention relates to a CO | 2012-01-26 |
20120021478 | MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF AROMATICS, 2,4-PENTADIENOATE AND 1,3-BUTADIENE - The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene. | 2012-01-26 |
20120021479 | Systems of Hydrogen Production in Bacteria - This invention relates to engineered bacterial systems such as engineered cyanobacterial systems and to methods of using these bacterial systems to generate hydrogen. | 2012-01-26 |
20120021480 | Use of Enzymes Having Silicase Activity - The present invention relates to the use of polypeptides having silicase activity for the modification or synthesis of silica, silicones and other silicium (IV) compounds. The present invention also relates to the use of polypeptides having silicase activity for the modification of glass, sand, asbestos, computer chips, glass wool, fiber glass, optical fibers and silicones, for the removal of sand from oil-sands, for the removal of asbestos, and for sandblasting. | 2012-01-26 |
20120021481 | ELECTROMECHANICAL LYSING OF ALGAE CELLS - Methods and electroporation devices for electrical treatment of algal cell cultures for release of lipids and proteins are described herein. The method of the present invention exploits the differences in electrical time constants for the media inside the cell and outside the cell to produce a net force to cause cellular lysis and extract cellular components. The method of the present invention can be used in the treatment of flocculated as well as unflocculated algal cell cultures. The device of the present invention provides efficient cell lysing in a low-energy cost set-up. | 2012-01-26 |
20120021482 | METHODS FOR ISOLATING VERY SMALL EMBRYONIC-LIKE (VSEL) STEM CELLS - The presently disclosed subject matter provides methods of isolating populations of stem cells that from bone marrow, peripheral blood, and/or other sources. Also provided are methods of using the stem cells for treating tissue and/or organ damage in a subject. | 2012-01-26 |
20120021483 | METHOD FOR PRODUCING LIPASE POWDER COMPOSITIONS - The present invention provides a method for producing a ground product of a lipase powder composition comprising an immobilized lipase in which a lipase derived from | 2012-01-26 |
20120021484 | Recombinant FcRn and Variants Thereof for Purification of Fc-Containing Fusion Proteins - The invention is directed to methods of purifying Fc-containing molecules using a soluble neonatal Fc receptor (sFcRn). Native FcRn binds Fc-containing proteins at or below about pH 6.5 and releases them at or above about pH 7 and provides a much milder approach for capturing and purifying Fc-containing proteins, in particular, therapeutic Fc-containing proteins. Other embodiments of the invention provide modifications to alter the pH for binding and elution to the sFcRn, to modulate Fc-containing protein binding affinity, to affect sFcRn linkage to a support surface, or to improve the stability of sFcRn to conditions utilized in the methods of the invention. | 2012-01-26 |
20120021485 | HYBRID ALPHA-AMYLASES - Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like ?-amylase, e.g., a | 2012-01-26 |
20120021486 | ENZYME-BASED NANOSCALE DECONTAMINATING COMPOSITES - The invention relates to decontaminating composites, and methods, compositions, and kits comprising the same. In some aspects, the invention relates to a decontaminating composite, comprising a perhydrolase associated with a carbon nanotube, that is useful for producing peracids. | 2012-01-26 |
20120021487 | METHOD OF TREATING GAUCHER DISEASE - Therapeutic compositions and methods for treatment of late-onset Gaucher disease are described herein. The compositions comprise compounds having activity as pharmacological chaperones for mutant forms of the beta-glucocerebrosidase. Methods of treatment involve providing therapeutically effective amounts of such compositions to subjects in need thereof. | 2012-01-26 |
20120021488 | THERMOTOLERANT NON-K12 ESCHERICHIA COLI PHYTASE AND ITS PRODUCTION - This invention relates to cloning and sequencing of thermotolerant phytase gene from Non-K12 | 2012-01-26 |
20120021489 | MICROBIAL ENZYMES AS DETERGENT ADDITIVES - Provided in part herein are compositions that include isolated microbial enzymes, such as amylases, lipases, and proteases that are useful as detergent additives. Also provided are methods of isolating amylases, lipases, and proteases from microbes, as well as methods of using these enzymes as detergent additives, and for stain removal. | 2012-01-26 |
20120021490 | GLUCANASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature. | 2012-01-26 |
20120021491 | IDENTIFICATION OF ANTIGEN OR LIGAND-SPECIFIC BINDING PROTEINS - The present invention discloses novel methods for the generation, expression and screening of diverse collections of binding proteins such as antibodies or fragments thereof in vertebrate host cells in vitro, for the identification and isolation of ligand- or antigen-specific binding proteins. The methods disclosed herein allow the expression of diverse collections of binding proteins from at least one vector construct, which optionally can give rise to collections of diverse binding proteins upon transfer and expression into vertebrate host cells in situ. | 2012-01-26 |
20120021492 | Cultivation and dispensing of bacteria - An apparatus | 2012-01-26 |
20120021493 | ORGANIC BIOFILM SUBSTRATA AS A MICROBIAL INOCULUM DELIVERY VEHICLE FOR BIOAUGMENTATION OF PERSISTENT ORGANIC POLLUTANTS IN CONTAMINATED SEDIMENTS AND SOILS - A system and methods for removal of persistent organic pollutants (POPs) from an environment, where the system includes an inert and organic biofilm substrata as biofilm media for dual use: 1) inoculation of microorganisms to degrade POPs and 2) accumulation of POPs on the substrata, effective in maintaining bioavailable concentrations for sustaining microbial activity. Microorganisms capable of degrading or transforming POPs are actively associated with the substrata as a biofilm. Application of this delivery vehicle will enhance the microbial degradation of POPs, while simultaneously adsorbing hydrophobic POPs from the environment making them bioavailable for the microorganisms located in the formed biofilms and additionally lowering the aqueous concentration of POPs that have detrimental effects towards fish and mammals as they bioaccumulate through the food chain. | 2012-01-26 |
20120021494 | ENHANCED PHOTOSYNTHESIS AND PHOTOCATALYSIS WATER TREATMENT/BIOMASS GROWTH PROCESS - A process utilizing enhanced photosynthesis and photocatalysis to purify water and/or utilize CO | 2012-01-26 |
20120021495 | GENERATION OF MATERIALS WITH ENHANCED HYDROGEN CONTENT FROM ANAEROBIC MICROBIAL CONSORTIA INCLUDING DESULFUROMONAS OR CLOSTRIDIA - An isolated microbial consortia is described. The consortia may include a first-bite microbial consortium that converts a starting hydrocarbon that is a complex hydrocarbon into two or more first-bite metabolic products. The consortia may also include a downstream microbial consortium that converts a starting hydrocarbon metabolic product into a downstream metabolic product. The downstream metabolic product has a greater mol. % hydrogen than the starting hydrocarbon. The first-bite microbial consortium or the downstream microbial consortium includes one or more species of | 2012-01-26 |
20120021496 | METHOD FOR CIRCULATORY CULTIVATING PHOTOSYNTHETIC MICROALGAE - A circulatory photobioreactor is provided. The circulatory photobioreactor comprises a first cultivating part, a second cultivating part and a pump part connecting the first cultivating part and the second cultivating part. The first cultivating part comprises a culture tank in which culture media are supplied and a first light source coupled to the culture tank, which illuminates the inside of the culture tank. The second cultivating part comprises a culture pipe placed outside of the culture tank and supplied with cultures from the culture tank and a second light source coupled to the culture pipe, which illuminates the inside of the culture pipe. The pump part is connected to both the first cultivating part and the second cultivating part in order to circulate the culture solution between them. | 2012-01-26 |
20120021497 | HYDRODYNAMIC EXTRACTION OF OILS FROM PHOTOSYNTHETIC CULTURES - The presently described invention relates to the method of extraction of oils from photosynthetic cultures using hydrodynamic cavitation technology for the production of biofuels or other products. This method is referred to herein as Hydrodynamic extraction. | 2012-01-26 |
20120021498 | PHOTOSYNTHETIC REACTOR FOR CULTIVATING MICROORGANISMS, AND METHOD FOR CULTIVATING MICROORGANISMS - A photosynthetic reactor ( | 2012-01-26 |
20120021499 | Method for removing pollutants in pollutants-contaminated soil - A method for removing pollutants in pollutants-contaminated soil is disclosed. The method includes following steps. A biodiversity system with microbial biodiversity characterization is used to induce growth and reproduce of at least one dominant synergistic flora for petroleum degradation. Most of the petroleum in the soil can be degraded when the temperature of the biodiversity system is below 30-50° C. The remained petroleum in the soil is than rapidly washed out from the soil through forming a petroleum-in-water emulsion by biosurfactant synthesized by the microorganisms in the biodiversity system. | 2012-01-26 |
20120021500 | BIOGAS PRODUCING SYSTEM - The present invention relates to a method for producing biogas by anaerobic digestion of organic matter. The method comprises: feeding organic matter suitable for biogas production to a first tank reactor | 2012-01-26 |
20120021501 | Cell Separation Method And Apparatus - Disclosed herein are apparatus and methods for isolating a fraction of interest from a physiological fluid sample. A sample holder for isolating a fraction of interest from a physiological fluid sample includes a flexible compartment and a rigid exoskeleton that supports the flexible compartment. The flexible compartment may have at least one reservoir with a height to volume ratio of about 0.1 cm/mL to about 5 cm/mL. An automated device for extracting a fraction of interest from the sample includes a sample holder with a flexible compartment supported by a rigid exoskeleton, a support for the sample holder connected to one or more fluid extraction devices, and a motor for moving the extraction device relative to the sample holder. The automated device may include an optical sensor and may include a clamp for clamping the flexible compartment. | 2012-01-26 |
20120021502 | SENSOR FOR FAST DETECTION OF E-COLI - A method of fabricating biochip sensor comprising providing a precursor; depositing the precursor on a substrate to form a coating; and rapid melting/quenching treatment of the coating with an energy source to form micro/nanotextured surface with enhanced reflectance for fast chemiluminescence response of | 2012-01-26 |
20120021503 | BIOMASS HYDROTHERMAL DECOMPOSITION APPARATUS, TEMPERATURE CONTROL METHOD THEREOF, AND ORGANIC RAW MATERIAL PRODUCTION SYSTEM USING BIOMASS MATERIAL - A biomass hydrothermal decomposition apparatus that feeds a solid biomass material | 2012-01-26 |
20120021504 | AERATED COMPOSTER AND WASTE COLLECTION BIN - An aerated compost and waste collection bin is disclosed. The bin includes a body portion having a bottom, at least one sidewall from the bottom, and an open top forming a container. A perforated tube extends upwards from the bottom of the body portion. At least one lateral vent tube is connected to the perforated tube and the sidewall of the body portion. The lateral vent tube allows air to circulate from outside the body portion of the bin through to the perforated tube. | 2012-01-26 |
20120021505 | CIRCULATORY PHOTOBIOREACTOR - A circulatory photobioreactor is provided. The circulatory photobioreactor comprises a first cultivating part, a second cultivating part and a pump part connecting the first cultivating part and the second cultivating part. The first cultivating part comprises a culture tank in which culture media are supplied and a first light source coupled to the culture tank, which illuminates the inside of the culture tank. The second cultivating part comprises a culture pipe placed outside of the culture tank and supplied with cultures from the culture tank and a second light source coupled to the culture pipe, which illuminates the inside of the culture pipe. The pump part is connected to both the first cultivating part and the second cultivating part in order to circulate the culture solution between them. | 2012-01-26 |
20120021506 | Regulatory sequences for expressing gene products in plant reproductive tissue - Expression cassettes causing specific regulatory control of transgene expression in plants, wherein the expression cassettes include regulatory sequences from the MADS gene family for expression of recombinant gene products in the reproductive tissue of plants for the purpose of generating abiotic stress tolerant plants. | 2012-01-26 |
20120021507 | BIOREACTOR AND USES THEREOF - Disclosed are bioreactor systems and methods to transfer a gaseous composition into a liquid composition in a closed environment. Also disclosed are bioreactor systems and methods for high-density culture of microorganisms and stably culture of activated sludge. | 2012-01-26 |
20120021508 | Method and Device for Identification of Nucleated Red Blood Cells from a Maternal Blood Sample - A method for concentrating and isolating nucleated cells, such as a maternal and fetal nucleated red blood cells (NRBC's), in a maternal whole blood sample. The invention also provides methods and apparatus for preparing to analyze and analyzing the sample for identification of fetal genetic material as part of prenatal genetic testing. The invention also pertains to methods and apparatus for discriminating fetal nucleated red blood cells from maternal nucleated red blood cells obtained from a blood sample taken from a pregnant woman. | 2012-01-26 |
20120021509 | ISOLATING METHOD FOR UMBILICAL CORD BLOOD-DERIVED PLURIPOTENT STEM CELLS EXPRESSING ZNF281 - The present invention relates to a method for isolating pluripotent/multipotent stem cells derived from umbilical cord blood, characterized by culturing monocytes isolated from umbilical cord blood in a culture vessel containing fibronectin and then harvesting stem cells from the culture, the umbilical cord blood-derived pluripotent/multipotent stem cells isolated thereby; and a cell therapeutic agent containing the pluripotent/multipotent stem cells derived from umbilical cord blood or cells differentiated therefrom. The present invention also relates to a novel culture media for stem cells, a culture method for stem cells which is characterized by culturing and proliferating stem cells in the culture media, and a method for increasing stemness of stem cells which is characterized by a sphere culture or a three-dimensional culture of stem cells. | 2012-01-26 |
20120021510 | METHOD FOR IMPROVED SINGLE CELL CLONING - The present invention relates to methods for the cultivation of a population of cells in a serum free cell culture medium, wherein the population of cells has a cell concentration of less than 100 cells/ml, wherein a serum free cell culture medium containing recombinant albumin and recombinant transferrin is used. | 2012-01-26 |
20120021511 | PREPRIMITIVE STREAK AND MESENDODERM CELLS - This disclosure relates to compositions comprising human preprimitive streak cells and/or human mesendoderm cells as well as methods for their production. Additionally, disclosed herein are methods of identifying factors useful in the further differentiation of preprimitive streak and mesendoderm cell types. | 2012-01-26 |
20120021512 | Interleukin-1 Alpha Antibodies and Methods of Use - Fully human monoclonal Abs includes (i) an antigen-binding variable region that exhibits very high binding affinity for IL-1α and (ii) a constant region that is effective at both activating the complement system though C1q binding and binding to several different Fc receptors. | 2012-01-26 |
20120021513 | SMALL MOLECULES SUPPORTING PLURIPOTENT CELL GROWTH AND METHODS THEREOF - The present invention relates to compositions and methods for maintaining undifferentiated pluripotent stem cell cultures. | 2012-01-26 |
20120021514 | HETEROGENEOUS POLYMERIC MICELLES FOR INTRACELLULAR DELIVERY - Compositions comprising a heterogeneous polymeric micelle and an agent (e.g., a polynucleotide) associated with the micelle are disclosed, together with methods for intracellular delivery of such agent. | 2012-01-26 |
20120021515 | OLIGOMERIC COMPOUNDS AND METHODS - The present invention provides oligomeric compounds and uses thereof. In certain embodiments, such oligomeric compounds are useful as antisense compounds. Certain such antisense compounds are useful as RNase H antisense compounds, as RNAi compounds, and/or as modulators of splicing. | 2012-01-26 |
20120021516 | NOVEL STRUCTURALLY DESIGNED shRNAs - Provided is an improved design of shRNA based on structural mimics of miR-451 precursors. These miR-451 shRNA mimics are channeled through a novel small RNA biogenesis pathway, require AGO2 catalysis and are processed by Drosha but are independent of DICER processing. This miRNA pathway feeds active elements only into Ago2 because of its unique catalytic activity. These data demonstrate that this newly identified small RNA biogenesis pathway can be exploited in vivo to produce active molecules. | 2012-01-26 |
20120021517 | Bacterial Mediated Delivery of Nuclear Protein Into Pluripotent and Differentiated Cells - A modified | 2012-01-26 |
20120021518 | GROWTH FACTOR HOMOLOG ZVEGF4 - Polypeptide growth factors, methods of making them, polynucleotides encoding them, antibodies to them, and methods of using them are disclosed. The polypeptides comprise an amino acid segment that is at least 70% identical to residues 52-179 of SEQ ID NO:2 or residues 258-370 of SEQ ID NO:2. Multimers of the polypeptides are also disclosed. The polypeptides, multimeric proteins, and polynucleotides can be used in the study and regulation of cell and tissue development, as components of cell culture media, and as diagnostic agents. | 2012-01-26 |
20120021519 | EFFICIENT INDUCTION OF PLURIPOTENT STEM CELLS USING SMALL MOLECULE COMPOUNDS - The disclosure features a method of producing a reprogrammed cell (e.g. an induced pluripotent stem cell or an undifferentiated cell) from a differentiated (e.g. somatic) cell. In some embodiments, the methods includes contacting a differentiated (e.g. somatic cell) with a TGFBR1 inhibitor or anti-TGF-β-antibody to produce a reprogrammed cell (e.g. pluripotent stem cell or undifferentiated cell). Embodiments of the present invention relate to a reprogrammed cell and methods and compositions for producing a chemically produced reprogrammed cell or populations thereof. | 2012-01-26 |
20120021520 | RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 2012-01-26 |
20120021521 | RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 2012-01-26 |
20120021522 | Method for the Evolutionary Design of Biochemical Reaction Networks - The present invention relates to methods for achieving an optimal function of a biochemical reaction network. The methods can be performed in silico using a reconstruction of a biochemical reaction network of a cell and iterative optimization procedures. The methods can further include laboratory culturing steps to confirm and possibly expand the determinations made using the in silico methods, and to produce a cultured cell, or population of cells, with optimal functions. The current invention includes computer systems and computer products including computer-readable program code for performing the in silico steps of the invention. | 2012-01-26 |
20120021523 | METHOD AND SYSTEM FOR CRYSTALLIZATION AND X-RAY DIFFRACTION SCREENING - An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm. | 2012-01-26 |
20120021524 | MICROSENSOR FOR MERCURY - Methods and devices for detecting a concentration of one or more element in hydrocarbon and/or natural gas in a oil and gas field application. The device including a microstructure having a low thermal mass suspended within a channel, the microstructure includes a supporting layer and a insulating layer; a controllable thermal device in communication with the supporting layer of the microstructure, wherein the controllable thermal device is controllably heated to one or more release temperature of the one or more element; a sensing layer arranged on the insulating layer to absorb molecules of the one or more element from hydrocarbon and/or natural gas; a detecting and measuring resistance device in communication with the sensing layer for measuring the resistance changes caused by absorption of molecules of the one or more element onto the sensing layer at a first temperature and a second temperature, and storing the data on a processor. | 2012-01-26 |
20120021525 | OPTICS COLLECTION AND DETECTION SYSTEM AND METHOD - Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described. | 2012-01-26 |
20120021526 | METHOD FOR ANALYSIS OF ISOTOPES IN BODILY FLUIDS - Biological fluid samples containing proteins (e.g., blood plasma or saliva) are prepared for isotopic analysis by precipitating the proteins while leaving the isotopic ratio unaffected. This precipitation can involve adding metal ions, salts, organic solvents, or organic polymers. The sample is then centrifuged to allow transfer of the supernatant for isotopic analysis, e.g. by tunable diode laser absorption spectrometry to obtain a quantitative measure of the | 2012-01-26 |
20120021527 | LAB-ON-A-CHIP FOR ALKALINITY ANALYSIS - Described is a lab-on-a-chip device and a method employing a lab-on-a-chip device for determining the concentration of species present in water. | 2012-01-26 |
20120021528 | Method for Adding an Apparent Non-Signal Line to a Rapid Diagnostic Assay - A test device and method for determining the presence or absence of one or more analytes in a fluid sample, the test device including a support or member bearing a mark thereon, and a matrix or member containing a capture zone. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (−) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (−) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user. | 2012-01-26 |
20120021529 | Micro-Valve and Micro-Fluidic Device Using Such - A micro-valve ( | 2012-01-26 |
20120021530 | HETEROGENEOUS FOLDAMERS CONTAINING alpha, beta, and/or gamma-AMINO ACIDS - Disclosed are isolated, unnatural polypeptides containing cyclically-constrained β-amino acid residues and cyclically-constrained γ-amino acid residues. The compounds are unnatural and because they contain rotationally constrained residues that are not amenable to enzymatic degradation, the compounds are useful to probe protein-protein and other large molecule interactions. | 2012-01-26 |
20120021531 | Assay Reader, Device and Method of Measuring HCG - Disclosed is a method for determining a quantitative estimate of the length of time since conception in a female mammalian subject, the method comprising: a)providing a liquid sample suspected of containing hCG; b)measuring, by means of an assay or assay device, an analyte measurement signal, whose value is dependent upon the level of hCG; c)comparing the measured signal value to an analyte threshold, wherein said analyte threshold corresponds to a time since conception; d)providing an quantitative estimate of the length of time since conception based upon the comparison in step (c). | 2012-01-26 |
20120021532 | Methods of Detecting and Treating Pulmonary Disease and Markers Thereof - The present invention relates to the treatment of pulmonary diseases. More specifically, the invention relates to new methods of detecting and treating chronic obstructive pulmonary disease (COPD). In particular, the invention relates to a method of measuring one or more lipid metabolites in human body fluids as an indicator/biomarker of the progress of chronic obstructive pulmonary disease. The present invention also relates to a method of detecting and/or monitoring chronic obstructive pulmonary disease in a subject, the method comprising measuring the level of at least one lipid metabolite in a sample from the subject, wherein said level is indicative of COPD. The present invention also relates to a method of assessing the efficacy of a COPD treatment in a subject, the method comprising a step of measuring the level of at least one lipid metabolite in a sample from the subject, wherein said level is indicative of COPD severity or status. | 2012-01-26 |
20120021533 | SENSITIVE METHOD TO ANALYSE FREE PEG-MALEIMIDE - The present invention relates generally to a novel method using HPLC and fluorescence detection of free PEG-mal in PEGylated proteins and PEG-mal raw materials by adding a fluorescent label to the free PEG-mal. | 2012-01-26 |
20120021534 | MARKERS FOR BRAIN DAMAGE - Methods to identify markers for brain damage using fresh brain tissue and methods and compositions for detecting these markers are disclosed. | 2012-01-26 |
20120021535 | MAGNETIC STACK WITH OXIDE TO REDUCE SWITCHING CURRENT - A magnetic stack having a ferromagnetic free layer, a metal oxide layer that is antiferromagnetic at a first temperature and non-magnetic at a second temperature higher than the first temperature, a ferromagnetic pinned reference layer, and a non-magnetic spacer layer between the free layer and the reference layer. During a writing process, the metal oxide layer is non-magnetic. For magnetic memory cells, such as magnetic tunnel junction cells, the metal oxide layer provides reduced switching currents. | 2012-01-26 |
20120021536 | METHOD AND SYSTEM FOR APPLICATION OF AN INSULATING DIELECTRIC MATERIAL TO PHOTOVOLTAIC MODULE SUBSTRATES - A method and related system are provided for depositing a dielectric material into voids in one or more of the semiconductor material layers of a photovoltaic (PV) module substrate. A first side of the substrate is exposed to a light source such that light is transmitted through the substrate and any voids in the semiconductor material layers on the opposite side of the substrate. The light transmitted through the voids is detected and a printer is registered to the pattern of detected light to print a dielectric material and fill the voids. | 2012-01-26 |
20120021537 | METHODS OF EVALUATING EPITAXIAL GROWTH AND METHODS OF FORMING AN EPITAXIAL LAYER - A method of evaluating an epitaxial growing process includes forming a mold layer on each of a plurality of substrates, forming a photoresist pattern on each mold layer, the photoresist pattern having opening portions, a total area of a bottom portion of the opening portions being different for each substrate, patterning each mold layer to expose a surface portion of the substrate to form an evaluation pattern on each substrate, evaluation patterns including opening portions corresponding to the opening portion in the photoresist pattern, determining substrate opening ratios for each substrate based on the opening portions in the evaluation pattern thereon, the substrate opening ratios being different for each substrate, performing a selective epitaxial process on each substrate to form an epitaxial layer, and evaluating characteristics of the epitaxial layer for each substrate to determine an optimal substrate opening ratio. | 2012-01-26 |
20120021538 | PLASMA PROCESSING METHOD AND STORAGE MEDIUM - There is provided a plasma processing method performing a plasma etching process on an oxide film of a target substrate through one or more steps by using a processing gas including a CF-based gas and a COS gas. The plasma processing method includes: performing a plasma etching process on the oxide film of the target substrate according to a processing recipe; measuring a concentration of sulfur (S) remaining on the target substrate (residual S concentration) after the plasma etching process is performed according to the processing recipe; adjusting a ratio of a COS gas flow rate with respect to a CF-based gas flow rate (COS/CF ratio) so as to allow the residual S concentration to become equal to or smaller than a predetermined value; and performing an actual plasma etching process according to a modified processing recipe storing the adjusted COS/CF ratio. | 2012-01-26 |
20120021539 | IN-LINE METROLOGY SYSTEM - A metrology system for gauging and spatially mapping a semiconductor material on a substrate can be used in controlling deposition and thermal activation processes. | 2012-01-26 |
20120021540 | SEMICONDUCTOR DEVICE AND METHOD FOR MANUFACTURING SEMICONDUCTOR DEVICE - A semiconductor device includes a plurality of semiconductor integrated circuits bonded to a structure body in which a fibrous body is impregnated with an organic resin. The plurality of semiconductor integrated circuits are provided at openings formed in the structure body and each include a photoelectric conversion element, a light-transmitting substrate which has stepped sides and in which the width of the projected section on a first surface side is smaller than that of a second surface, a semiconductor integrated circuit portion provided on the second surface of the light-transmitting substrate, and a chromatic color light-transmitting resin layer which covers the first surface and part of side surfaces of the light-transmitting substrate. The plurality of semiconductor integrated circuits include the chromatic color light-transmitting resin layers of different colors. | 2012-01-26 |
20120021541 | LIGHT EMITTING DEVICE AND METHOD OF FABRICATING THE SAME - A method of fabricating a light emitting device initially forms a copper clad ceramic board of the light emitting device using hot-pressing technique at high temperature and photolithography process. Next, a circuit of the light emitting device is formed using die bonding and wire bonding/flipchip processes. Finally, the light emitting device is sealed using transfer molding or injection molding process. | 2012-01-26 |
20120021542 | METHOD OF PACKAGING LIGHT EMITTING DEVICE - A method of packaging a light emitting device includes the following steps: providing a base; forming a mask on the base, the mask defining a plurality of holes therein; positioning a certain amount of glue in each of the holes; securing a film on the mask, a plurality of light emitting elements being positioned on the film, and each of the light emitting elements being positioned in a corresponding hole and adhered by the glue in the corresponding hole; separating the light emitting elements from the film and removing the film from the mask; and removing the mask from the base. | 2012-01-26 |
20120021543 | LIGHT EMITTING DEVICES WITH IMPROVED LIGHT EXTRACTION EFFICIENCY - Light emitting devices with improved light extraction efficiency are provided. The light emitting devices have a stack of layers including semiconductor layers comprising an active region. The stack is bonded to a transparent lens having a refractive index for light emitted by the active region preferably greater than about 1.5, more preferably greater than about 1.8. A method of bonding a transparent lens to a light emitting device having a stack of layers including semiconductor layers comprising an active region includes elevating a temperature of the lens and the stack and applying a pressure to press the lens and the stack together. Bonding a high refractive index lens to a light emitting device improves the light extraction efficiency of the light emitting device by reducing loss due to total internal reflection. Advantageously, this improvement can be achieved without the use of an encapsulant. | 2012-01-26 |
20120021544 | DISPLAY DEVICE, METHOD FOR MANUFACTURING DISPLAY DEVICE, AND SOI SUBSTRATE - A manufacturing method is provided which achieves an SOI substrate with a large area and can improve productivity of manufacture of a display device using the SOI substrate. A plurality of single-crystalline semiconductor layers are bonded to a substrate having an insulating surface, and a circuit including a transistor is formed using the single-crystalline semiconductor layers, so that a display device is manufactured. Single-crystalline semiconductor layers separated from a single-crystalline semiconductor substrate are applied to the plurality of single-crystalline semiconductor layers. Each of the single-crystalline semiconductor layers has a size corresponding to one display panel (panel size). | 2012-01-26 |
20120021545 | METHOD OF MANUFACTURING VERTICAL LIGHT EMITTING DIODE - A method of manufacturing a vertical light emitting diode includes: providing a first substrate; forming a lapping stop layer on the first substrate, the lapping stop layer being harder than the first substrate; depositing an epitaxial layer on the lapping stop layer; bonding a second substrate on the epitaxial layer; and removing the first substrate from the lapping stop layer. | 2012-01-26 |