Class / Patent application number | Description | Number of patent applications / Date published |
530400000 | Metal containing, e.g., chromoproteins, ferritin, ferredoxins, etc. | 21 |
20080242847 | Position dependent recognition of GNN nucleotide triplets by zinc fingers - The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity. | 10-02-2008 |
20080306248 | Conjugate of Biomacromolecule with Bioreductive and Preparative Method Thereof - Conjugate of biomacromolecule with bioreductive which can be useful for treating tumor is provided. The biomacromolecule is selected from apo-transferrin, Fe-transferrin, Ru-transferrin, Ti-transferrin, Ga-transferrin, Pt-transferrin, somatostatin, EGF, folacin acid or transcobalamin, and the bioreductive agent is selected from quinones, aromatic nitrogen oxides, fatty nitrogen oxides, heterocyclic nitro compound, transition metal compound. Such conjugate can selectively target the tumor cells, and lower the toxicity of medicines and survivability of tumor cells, so that the conjugate can be used for delivery of anti-tumor compounds or treating tumors. | 12-11-2008 |
20090131640 | PHOTOLUMINESCENT METAL COMPLEXES FOR PROTEIN STAINING - A method of staining a poly(amino acid) by contacting a poly(amino acid) with a complex of at least one of transition metal ion, and a plurality of donor ligands each of which is fully coordinated to the transition metal ion and is either a nitrogen donor ligand or a cyclometalated donor ligand, such that at least one of the donor ligands is a cyclometalated donor ligand. Donor ligands in a single complex can be the same or different. Nitrogen donor ligands will contain heteroaryl ring systems having from 10 to 40 ring atoms, where from 2 to 8 ring atoms are N, O, S, or combinations thereof, such that at least two ring atoms are N, wherein each nitrogen donor ligand is substituted with from 0 to 4 R | 05-21-2009 |
20090306350 | NEW PURIFICATION METHOD OF LACTOFERRIN - The invention relates to methods for purifying lactoferrin, stabilizing it in solution and improving its activity. In one embodiment of the present invention, it is provided methods for lactoferrin purification employing hydrophobic and/or hydrophilic adsorbent under specific conditions for maintaining or preserving lactoferrin protein stability. It is also provided a process to remove inhibitor of lactoferrin activity. | 12-10-2009 |
20100029910 | NANOGRAPHITE STRUCTURE/METAL NANOPARTICLE COMPOSITE - The present invention makes it possible to efficiently recognize carbon nanotubes, carbon nanohorns or modifiers thereof and to support functional compounds by fusing the ability of ferritin molecules capable of forming nanoparticles of inorganic metal atoms or inorganic metal compounds. In addition, because ferritin molecules are capable of forming two-dimensional crystals at the interface, the present invention makes it possible to align carbon nanotubes, carbon nanohorns with the use of the molecular arrangement ability of ferritin fused with nanographite structure recognition peptides. A nanographite structure/metal nanoparticle composite is constructed, wherein a nanoparticle of an inorganic metal atom or an inorganic metal compound is retained in an interior space of a protein in which a nanographite structure recognition peptide is fused or chemically bound to a surface of a cage protein such as ferritin, and wherein a plurality of nanoparticles of an inorganic metal atom or an inorganic metal compound are supported on a nanographite structure with the use of affinity of the nanographite structure recognition peptide to the nanographite structure. | 02-04-2010 |
20100121038 | Template-directed assembly of receptor signaling complexes - Transmembrane receptors in the signaling pathways of bacterial chemotaxis systems influence cell motility by forming noncovalent complexes with the cytoplasmic signaling proteins to regulate their activity. The requirements for receptor-mediated activation of CheA, the principal kinase of the | 05-13-2010 |
20110118449 | FERRITIN 2 FOR THE HOST IMMUNIZATION AGAINST TICKS - Ferritin 2 is a tick secreted protein, which serves in the tick plasma as a non-heme iron transporter from the gut to the peripheral tissues. It can be exploited as an antigen for raising up host antibodies, which can reversely block this antigen in the tick and the attached tick will not have functional mechanism of the non-heme iron transport from the gut to the peripheral tissues. This mechanism is essential for the further tick development and its blocking eventually prevents transmission of tick-borne pathogens. | 05-19-2011 |
20120116061 | METHOD OF ARRAYING FERRITIN - A method of selectively arraying ferritin and inorganic particles on a silicon oxide substrate at regions having vanadium, niobium or tantalum. An aspect of the method includes steps of: preparing a solution which contains ferritin modified at an N-terminal part of a subunit with a peptide set out in SEQ ID NO: 1, and from 0.01 v/v % to 10 v/v % of a nonionic surfactant and having a pH of from 7.4 to 8.2; and a binding step of bringing the solution in contact with regions of the substrate having vanadium, niobium, or tantalum to selectively array peptide-modified ferritin to vanadium, niobium or, tantalum portion. The method may also include a step of selectively arraying ferritin modified with the peptide set out in SEQ ID NO: 1, and the inorganic particles contained in ferritin at the vanadium, niobium, or tantalum portion by removing the solution. | 05-10-2012 |
20120130053 | SOLID PHASE GOLD NANOPARTICLE SYNTHESIS - A method of synthesizing ligand-conjugated gold nanoparticles (AuNPs) is disclosed. The method comprises: a) providing an amine-modified silica particle; b) providing a solution comprising Au | 05-24-2012 |
20130245240 | Bio-Hybrid Material, Production Method Therefor, and Stent - Provided is a bio-hybrid material that does not cause elution of nickel ions and has an excellent endothelialization ability, a production method therefor, and a stent. The bio-hybrid material ( | 09-19-2013 |
20130289252 | COMPOSITIONS AND METHODS FOR THE DELIVERY OF NITRIC OXIDE - H-NOX proteins are mutated to exhibit improved or optimal kinetic and thermodynamic properties for blood gas NO delivery. The engineered H-NOX proteins comprise mutations that impart altered NO or O | 10-31-2013 |
20130289253 | REVERSIBLE PROTEIN MULTIMERS, METHODS FOR THEIR PRODUCTION AND USE - Some aspects of this invention are based on the recognition that reversible protein multimers in which monomeric proteins are conjugated to a carrier molecule via chelation complex bonds are stable under physiological conditions and can be dissociated in a controlled manner under physiological, nontoxic conditions. Accordingly, such protein multimers are useful for a variety of in vitro, ex vivo, and in vivo application for research, diagnostics, and therapy. Some aspect of this invention provide reversible MHC protein multimers, and methods of using such multimers in the detection and/or isolation of specific T-cells or T-cell populations. Because reversible MHC multimers can efficiently be dissociated, the time of MHC binding to T-cell receptors, and, thus, T-cell receptor-mediated T-cell activation can be minimized. The use of reversible MHC multimers as provided herein, accordingly, allows for the detection and isolation of bona fide antigen-specific CD8+ T cells without inducing activation dependent cell death, including rare, therapeutically valuable T-cells expressing T-cell receptors binding tumor antigens with high affinity. Methods for the production and use of reversible multimers are also provided. | 10-31-2013 |
20140011983 | SMALL MOLECULE CONJUGATES WITH DIMETAL SPECIES FOR PROTEIN INHIBITION - Methods for targeting a protein by providing an inhibitor covalently linked to a rhodium(II) complex, introducing the inhibitor to the target protein and allowing the inhibitor and protein to interact. The rhodium(II) complex covalently linked to the inhibitor binds the target protein both inorganically and organically and forms stabilizing secondary contacts between the rhodium(II) complex and the protein. | 01-09-2014 |
20140058069 | PARTICLES AND OTHER SUBSTRATES USEFUL IN PROTEIN PURIFICATION AND OTHER APPLICATIONS - The present invention generally relates to particles, including microgel particles, for purifying proteins and other species. In one aspect, the particles comprise a metal-chelating moiety, which may be distributed substantially evenly throughout the particle in certain embodiments. In some cases, the particles may be porous, and in some embodiments, the particles may be made sufficiently small, for example, in order to form a microgel containing the particles. Such particles may be useful, for example, in binding metal ions (for example, nickel ions) using the metal-chelating moieties. In some embodiments, such particles may also be used to bind certain analytes (for example, proteins) containing tags which attract metal ions, for example, histidine tags. Accordingly, in certain embodiments, the particles may be used for binding or trapping proteins. In some cases, this process is reversible; for example, upon exposure of the particles to a histidine competitor, proteins or other analytes containing the histidine tags may be released form the particles. Other aspects of the invention are generally directed to methods of using such particles, methods of forming such particles, kits including such particles, or the like. | 02-27-2014 |
20140194602 | Metamaterial Optical Elements Self-Assembled on Protein Scaffolds - A genetically modified cowpea mosaic virus (CPMV) protein capsid serves as a scaffold for metal nanoparticles, preferably gold nanospheres, of 15 nm to 35 nm, creating plasmonic nanoclusters. The self-assembled nanoclusters gave rise to a 10-fold surface-averaged enhancement of the local electromagnetic field. Other viral capsids or virus-like proteins may also serve as such scaffolds. | 07-10-2014 |
20140256918 | METHOD FOR IMMOBILIZING MEMBRANE PROTEINS ON SURFACES - Disclosed herein are methods for immobilizing membrane proteins or membrane protein complexes on analytical surfaces, which in some aspects comprise: obtaining a membrane protein or membrane protein complex comprising a capture moiety; immobilizing the membrane protein or membrane protein complex on the analytical surface by means of the capture moiety; and stabilizing at least one of the secondary, tertiary, or quaternary structures of the immobilized membrane protein or membrane protein complex by crosslinking with a crosslinking reagent. Also disclosed are analytical surfaces, which in some aspects comprise: a membrane protein or membrane protein complex comprising a capture moiety, wherein the membrane protein or membrane protein complex is immobilized on the analytical surface by means of the capture moiety, and wherein at least one of the secondary, tertiary, or quaternary structures of the membrane protein or membrane protein complex is stabilized by crosslinking. | 09-11-2014 |
20150307545 | ADJUVANTING MATERIAL - The present invention provides an adjuvanting material, the adjuvanting material comprising a lipid dendritic cell targeting moiety to which is covalently linked a metal chelating group. Further, the present invention provides an immunogenic composition comprising (a) a lipid dendritic cell targeting moiety to which is covalently linked a metal chelating group; (b) an antigen comprising a metal affinity tag; and optionally (c) metal ions, whereby the antigen is linked to the lipid dendritic cell targeting moiety via the interaction between the metal affinity tag and the metal chelating group. | 10-29-2015 |
20160017059 | ADSORBENT - An adsorbent of the present invention includes a support; and a protein having an adsorption site for adsorbing a target substance, in which the protein includes a reversible binding site and a covalent binding site, the support includes an orientation-controlling site forming a reversible bond with the reversible binding site, and an immobilization site forming a covalent bond with the covalent binding site, the covalent binding site includes a nucleophilic functional group, and the immobilization site includes a functional group capable of reacting with the nucleophilic functional group by a nucleophilic substitution or a nucleophilic addition. Thus, it is possible to provide an adsorbent in which the utilization efficiency of the adsorption site of the protein is improved. As a result, the protein amount can be reduced, and the step for storing a culture solution can be simplified due to the faster purification step. | 01-21-2016 |
530401000 | Cytochromes, e.g., chlorophyl, orgotein, etc. | 3 |
20110130550 | PROTEIN MONOMER, PROTEIN POLYMER OBTAINED FROM SAID MONOMER, AND DEVICE THAT CONTAINS THEM - A protein polymer having a larger molecular weight is provided by regularly arranging a protein having a large molecular weight. The protein polymer having a large molecular weight can be obtained using a protein monomer represented by formula (I) or a salt thereof: | 06-02-2011 |
20120277414 | PROTEIN PHOTOELECTRIC TRANSDUCER AND TIN-SUBSTITUTED CYTOCHROME c - There are provided a novel protein which has extremely high stability with respect to light irradiation, and is capable of maintaining a photoelectric conversion function for a long time, and a protein photoelectric transducer which uses the protein, and is capable of being stably used for a long time. A tin-substituted horse-heart cytochrome c is obtained by substituting tin for iron as a central metal of a heme of a horse-heart cytochrome c. A tin-substituted bovine-heart cytochrome c is obtained by substituting tin for iron as a central metal of a heme of a bovine-heart cytochrome c. A protein made of the tin-substituted horse-heart cytochrome c or the tin-substituted bovine-heart cytochrome c is immobilized on an electrode to form a protein-immobilized electrode. A protein photoelectric transducer is formed with use of the protein-immobilized electrode. | 11-01-2012 |
20130072668 | METHOD FOR PRODUCING HYDROXYLATED ADAMANTANE USING CYTOCHROME P450 - Disclosed is a method for producing a hydroxylated form of a compound having an adamantane skeleton, which is useful as an intermediate for functional resins and pharmaceutical products, with high yield and at low cost. Specifically, a hydroxylated form of a compound having an adamantane skeleton can be obtained by using cytochrome P450. More specifically, an N-(5-hydroxy-2-adamantyl)-benzamide derivative can be produced by hydroxylating an N-(2-adamantyl)-benzamide derivative. | 03-21-2013 |