Class / Patent application number | Description | Number of patent applications / Date published |
435280000 | Resolution of optical isomers or purification of organic compounds or composition containing same | 13 |
20090280558 | Process For Dynamic Kinetic Resolution (DKR) Of Racemic Compounds In (Hydro) Fluorocarbon Solvents - A process for preparing a third compound stereo-selectively which process comprises reacting a substrate comprising a first enantiomeric compound with a racemisation catalyst to give a second enantiomeric compound, and concurrently reacting the second enantiomeric compound in the presence of a biological catalyst to give the third compound, wherein the process is performed in a solvent comprising at least one (hydro) fluorocarbon. The biological catalyst is preferably an enzyme. The substrate preferably comprises a racemate of the first and second enantiomeric compounds. The second enantiomeric compound may be reacted with a reagent, such as acyl donor, to form the third compound in the presence of the biological catalyst. | 11-12-2009 |
20100075404 | PROCESSES FOR THE DIGESTION OF COLANIC ACID - The present disclosure generally relates to processes employing polypeptides having colanic acid-degrading activity. The processes generally involve contacting a biological material with a polypeptide capable of digesting colanic acid. Additional process steps, such as chromatographic separation steps, are also described. | 03-25-2010 |
20100173392 | ROOM TEMPERATURE ELUTION OF NUCLEIC ACIDS - The present invention provides compositions and methods for efficient recovery of nucleic acids, in particular DNA, from storage media. The present invention provides a method for using an alkaline elution buffer and mechanical agitation to elute nucleic acids from dried samples on storage media at room temperature. In particular, the present invention provides a method for room temperature recovery of DNA, including double stranded DNA (dsDNA) and single stranded (ssDNA), from storage media. The eluted DNA is suitable for numerous downstream applications | 07-08-2010 |
20100240116 | LOVASTATIN ESTERASE ENZYME IMMOBILIZED ON SOLID SUPPORT, PROCESS FOR ENZYME IMMOBILIZATION, USE OF IMMOBILIZED ENZYME, BIOCATALYTIC FLOW REACTOR AND PROCESS FOR PREPARATION AND/OR PURIFICATION OF SIMVASTATIN - The invention relates to the lovastatin esterase enzyme immobilized on a solid support insoluble in water, the enzyme being covalently bound to a solid support activated with an at least difunctional coupling reagent, the immobilized lovastatin esterase exhibiting at least 5 times higher the hydrolytic activity towards lovastatin and salts thereof, in the presence of simvastatin and/or salts thereof, than towards simvastatin and salts thereof. The invention also relates to a process for immobilization of the lovastatin esterase enzyme on a solid support insoluble in water, and use of the enzyme immobilized on a solid support for preparation and/or isolation and/or purification of simvastatin, and also to a process for preparation and/or purification of simvastatin comprising treating the solution of the simvastatin salt containing residual content of the lovastatin salt with the lovastatin esterase enzyme until hydrolysing lovastatin to form the triol; separating the triol; and isolating simvastatin substantially free from lovastatin, wherein the solution of the simvastatin salt containing residual content of the lovastatin salt, is brought into a contact with the lovastatin esterase enzyme immobilized on a solid support insoluble in water. Also, the invention relates to a biocatalytic flow reactor with a bed, comprising a body ( | 09-23-2010 |
20100248337 | PROCESS FOR THE PRODUCTION OF (s)-5-CHLORO-2-ISOPROPYLPENT-4-ENOIC ACID ESTERS - A process for preparing a compound of formula (1) in enantiomerically enriched form, which comprises selective hydrolysis of the corresponding racemic compound (2) catalysed by an enzyme derived from a non-mammalian source and having esterase or lipase activity, wherein R is methyl or C | 09-30-2010 |
20110065168 | Preparation of Pregabalin and Related Compounds - Materials and methods for preparing (S)-(+)-3-aminomethyl-5-methyl-hexanoic acid and structurally related compounds via enzymatic kinetic resolution are disclosed. | 03-17-2011 |
20110070630 | METHOD FOR DERACEMIZATION OF ENANTIOMER MIXTURES - The invention relates to a process for enzymatic deracemization of enantiomer mixtures of secondary alcohols by a combination of oxidation and reduction reactions by means of stereoselective alcohol dehydrogenases and the cofactors thereof, wherein one enantiomer of an optically active secondary alcohol is in a formal sense selectively oxidized to the corresponding ketone, which is subsequently reduced selectively to the optical antipode, while the reduced form of the cofactor is provided for the reduction reaction by means of an additional enzyme, characterized in that two alcohol dehydrogenases with opposite stereoselectivity and different cofactor selectivity and the two corresponding, different cofactors are used for the oxidation and reduction reactions, and the oxidized and reduced cofactors are interconverted in a parallel enzymatic reaction with the additional enzyme, the direction of the deracemization toward one of the two enantiomers being controllable by the selection of the two alcohol dehydrogenases or using the selectivity difference of the additional enzyme for the two cofactors. | 03-24-2011 |
20120028340 | KINETIC RESOLUTION OF (4S) -- 4- PHENYL -- 3- [(5RS)-5-(4-FLUROPHENYL)-5-HYDROXYPENTANOYL] --1,3-OXAZOLIDIN-2-ONE TO THE (5S) ISOMER VIA LIPASECATALYZED ENANTIOSELECTIVE ESTERIFICATION OF THE (5R) ISOMER - A process for synthesis of 4S-phenyl-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-1,3 oxazolidin 2-one comprising resolution of 4S-phenyl-3-[(5RS)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-1,3 oxazolidin 2-one by selective esterification of 4S-phenyl-3-[(5R)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-1,3 oxazolidin 2-one using appropriate esterification reagent in an organic solvent in presence of Lipase enzyme at a temperature ranging from 0° to 100° C., and further isolation. | 02-02-2012 |
20120064611 | Process For The Preparation of (3R)-Hydroxybutyl (3R) -Hydroxybutyrate By Enzymatic Enantioselective Reduction Employing Lactobacillus Brevis Alcohol Dehydrogenase - A process for producing a compound which is (3R)-hydroxybutyl(3R)-hydroxybutyrate of formula (I) which process comprises submitting, to enantioselective reduction, a compound of the following formula (II), (III) or (IV). | 03-15-2012 |
20130122574 | METHOD FOR PURIFYING A TARGET NUCLEIC ACID - The present invention pertains to method for purifying at least a target nucleic acid from a sample, said method comprising at least the following steps: a) incubating the sample with at least one protein-degrading compound; b) binding the target nucleic acid to a solid phase; c) eluting the target nucleic acid from the solid phase; d) incubating the eluted target nucleic acid with at least one protein-degrading compound; e) binding the target nucleic acid again to a solid phase; f) optionally eluting the bound target nucleic acid from the solid phase. It was surprisingly found that performing a second protein digestion step after the target nucleic acid was bound and eluted from a solid phase before the nucleic acids are rebound to a solid phase is very efficient in reducing remaining protein contaminations in the isolated nucleic acid. | 05-16-2013 |
20140154784 | Method and Process for the Degradation of Cyclic Ethers in Ethoxylate-Containing Actives - The invention provides a method and process for the degradation of cyclic ethers that are often found in liquid streams comprising cleaning actives, through the addition of cyclic ether-degrading enzymes or enzyme-producing microbes. The cleaning actives that may be successfully treated by the methods described are selected from among anionic surfactants, nonionic surfactants, solvents, derivatives thereof, as well as mixtures of any of the foregoing. | 06-05-2014 |
20140212957 | PRODUCTION OF OPTICALLY PURE PROPANE-1,2-DIOL - A method for producing optically pure propane-1,2-diol, including the method steps: a. hydrogenation of lactides, metal-catalysed heterogenous catalysis being carried out in the presence of hydrogen, a crude product containing propane-1,2-diol being produced, and b. dynamic, kinetic racemate resolution, propane-1,2-diol of an optical purity in the range of ≧99% e.e. being produced. | 07-31-2014 |
20150336999 | PROCESS FOR THE PRODUCTION OF SOPHOROSE STARTING FROM SOPHOROLIPIDS - The present invention concerns a process for the production of sophorose starting from sophorolipid, in which a step for the hydrolysis of sophorolipid is carried out in an aqueous medium in the presence of an acidic compound. | 11-26-2015 |