Entries |
Document | Title | Date |
20080199929 | Method and System For Collecting Cells Following Laser Microdissection - A method of collecting target regions from a target object is described. The method in one embodiment comprises mounting a negatively-charged membrane on a first side of a substrate, mounting a target object on the membrane, positioning a collection material adjacent to the target object, and passing a laser beam from a second side of the substrate, through the substrate, the membrane, and the target object, to dissect target regions from the prepared tissue section, whereby the dissected target regions adhere to the collection material. In another embodiment, the present invention is a system for collecting target regions from a target object. In one embodiment, the system comprises a substrate having a first side and a second side, a negatively-charged membrane adhered to the first side of the substrate, and a collection material mountable adjacent to the membrane. In another embodiment, the system further comprises an inverted microscope, a stage for holding the substrate over the microscope, a generator operable to generate a laser beam to pass through the substrate from the second side and to dissect target regions from a target object mounted on the membrane, whereby the dissected target regions adhere to the collection material. In the preferred embodiments, the target objects are tissues and the target regions are cells. | 08-21-2008 |
20080199930 | METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER - A method and apparatus for rapid disruption of cells or viruses using beads and a laser are provided. According to the method and apparatus for rapid disruption of cells or viruses using beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC. | 08-21-2008 |
20080206828 | Device For Introducing Substance Into Cell, Cell Clamping Device and Flow Path Forming Method - A device for introducing a substance into a cell which can realize a high-efficient external substance introduction by means of electro-poration not depending on a cell size, a cell clamping device capable of clamping a cell at many locations, and a flow path forming method capable of efficiently forming a flow path. The device for introducing a substance into a cell ( | 08-28-2008 |
20080213853 | Magnetofluidics - Magnetofluidic systems and techniques. In one aspect, a magnetofluidic device includes a superhydrophobic surface and a fluid sample in physical contact with the superhydrophobic surface, the fluid sample comprising a collection of particles coated with a passivating layer. The particles are magnetically active in that they respond to an applied magnetic field. | 09-04-2008 |
20080220491 | Method and device for electroporation of biological cells - Described are methods for electrical treatment of biological cells, in particular using electrical field pulses, involving the steps: arrangement of the cells ( | 09-11-2008 |
20080227168 | METHODS AND MATERIALS FOR EXTRA AND INTRACELLULAR DELIVERY OF CARBON NANOTUBES - The present invention includes compositions and methods to deliver carbon nanostructures that include agents for delivery to cells, wherein the carbon nanostructure and the agent are made soluble by coating the carbon nanostructure with one or more polymers, e.g., low band gap conductive polymers. | 09-18-2008 |
20080233625 | Dietary supplements for regulating the central nervous system - Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells have been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength for a period of time sufficient to increase the capability of said plurality of yeast cells to regulate the central nervous system. Also included are methods of making such compositions. | 09-25-2008 |
20080233626 | Enhanced broad-spectrum UV radiation filters and methods - The present invention obtains from the discovery that combining traditional UV pigments, organic chemicals, or both, combined with NA, creates a broad spectrum UV absorbing additive that is much more efficient than using any of the ingredients by themselves. Methods for producing NA-coated particles as a UV protection additive to paints, fiberglass, plastic, polymers, siloxanes/silicates/reactive silanols, sealants or other film forming coatings or penetrating fluids and solid articles are contemplated in this invention as well as the coatings, sealants and other protectants and the coated and/or finished articles themselves. | 09-25-2008 |
20080299636 | SYSTEM AND METHOD FOR INACTIVATING MICROORGANISMS WITH A FEMTOSECOND LASER - The invention relates to a method of inactivating or diminishing the activity of microorganisms and an apparatus implementing that method. Microorganism activity is diminished through exciting the vibrational state of a microorganism with pulses of radiation femtoseconds in width. The wavelength of the pulses is in a range of the electromagnetic spectrum to which water is substantially transparent such as visible light. The pulses cause the microorganisms to vibrate such that their activities become diminished. A laser produces the pulses. A harmonic generator then acts upon the pulses to produce a scattering effect that is used to irradiate the microorganisms. One such laser is a titanium sapphire laser. One example of a harmonic generator is a nonlinear crystal such as a BBO crystal. The apparatus may also have a focusing lens such as a microscope objective that focuses the beam on a microorganism. The invention is effective for the inactivation of viruses or bacteria. The present invention may be utilized in order for extracting nucleic acid from microorganisms. The invention may be used in the manufacturing of vaccines. The invention enables the selective inactivation of target viruses and bacteria without causing cytotoxicity in mammalian cells. | 12-04-2008 |
20090017516 | TARGET PHYSIOLOGICAL FUNCTION INACTIVATOR USING PHOTOSENSITIZER-LABELED FLUORESCENT PROTEIN - An object of the present invention is to provide a method of generating reactive oxygen species in a light irradiation-dependent manner, so as to inactivate any target physiological function. The present invention provides a target physiological function inactivator which consists of a photosensitizer-labeled fluorescent protein, wherein fluorescence resonance energy transfer (FRET) from the fluorescent protein to the photosensitizer occurs as a result of light irradiation, so that the photosensitizer can be excited to generate reactive oxygen species. | 01-15-2009 |
20090023194 | DEVICE AND METHOD FOR THE CULTIVATION AND GENERATION OF BIOLOGICAL MATERIAL IN A NUTRIENT MIST - The present invention relates to a device and a method for the cultivation and generation of biological material. | 01-22-2009 |
20090061496 | Encapsulation of bacteria and viruses in electrospun fibers - A method of preserving organisms in viable form, the method comprising: suspending organisms in a solution of electrospinnable polymer; drawing droplets of said solution through a spinneret; applying an electrostatic field to said droplets under electrospinning conditions; so as to form fibers having a diameter no greater than about 5 μm within which distinct organisms are encapsulated in viable form. | 03-05-2009 |
20090137017 | GENERATION AND ISOLATION OF ANTIGEN-SPECIFIC T CELLS - The present invention relates generally to methods for generating, expanding, and isolating antigen-specific T cells. Compositions of antigen-specific T cells activated and expanded by the methods herein are further provided. | 05-28-2009 |
20090137018 | MAGNETIC THREE-DIMENSIONAL CELL CULTURE APPARATUS AND METHOD - A culture apparatus and method for growing cells and tissue in a three-dimensional configuration harnesses magnetic, paramagnetic, ferromagnetic and diamagnetic forces. The cells or tissue are grown with magnetized core particles and are suspended via magnetic forces in a native, non-restricted, three-dimensional configuration while being maintained in a normal gravity (1 g) growth environment in the absence of rotational alteration of the gravity vector. | 05-28-2009 |
20090215143 | Controlled alteration of pores using fluid flow and fabrication of articles thereby - The invention relates to microscopic structures and methods of making and using the structures. A method of forming a microscopic structure of a material includes obtaining a solution ( | 08-27-2009 |
20090239281 | Moving A Small Object in A Direction - For directional motion of a small object, a combination of at least two different properties is imparted to the object. At least one of the properties being responsive to an external influence to produce a force or torque acting on the object. At least another of the properties is responsive to the force or torque to cause a directional motion of the object. | 09-24-2009 |
20090280549 | Method for Treating Body Fluid - The invention provides a body fluid treatment method for selective ex vivo killing of malignant lymphoma cells, leukemia cells or activated macrophages in a body fluid, the body fluid treatment method comprising: an addition step wherein a compound represented by formula (I) below is added to a body fluid containing malignant lymphoma cells, leukemia cells or activated macrophages that has been removed from the body, to yield an addition mixture; and an excitation step wherein the addition mixture is irradiated with excitation light to excite the compound. According to the invention, there is provided a method for selective ex vivo killing of malignant lymphoma cells, leukemia cells or activated macrophages in a body fluid while avoiding adverse effects on normal cells. | 11-12-2009 |
20090311765 | Alginate poly-L-Lysine encapsulation as a technology for controlled differentiation of embryonic stem cells - Alginate polyelectrolyte encapsulation is used for the controlled differentiation of embryonic stem cells. An isolated cell population is provided. The cell population includes a single cell suspension of ES cells encapsulated within an alginate polyelectrolyte microenvironment. The encapsulated ES cells are capable of differentiating within said microenvironment into hepatocyte lineage cells in the absence of embryoid body intermediates or growth factor supplementation. | 12-17-2009 |
20090311766 | APPARATUS AND METHOD FOR HYDROLYZING BIOLOGICAL MATERIAL - Apparatus and method for hydrolyzing biological material for safe disposal thereof without the necessity of incineration or use of disinfectants are described. An alkaline solution having a concentration and an amount effective for hydrolyzing the biological material is brought into contact therewith by means of rotating paddles which both pound the biological material into small pieces and thoroughly mix the alkaline solution with the material under pressure and at elevated temperature. Following the hydrolysis of the biological material, a chosen portion of the water is removed from the alkaline solution and from the liquefied biological material, such that the resulting product solidifies when cooled. The present safe disposal of the biological material does not require incineration thereof, the addition of disinfectants thereto, or the discharge of liquid effluent containing processed biological material into the sewage system. | 12-17-2009 |
20090325256 | METHOD FOR CELL PATTERNING - Provided is a method for cell patterning, using an electrode substrate including a plurality of electrodes and a cell culture substrate disposed so as to face the electrode substrate, the method comprising the steps of:
| 12-31-2009 |
20100021982 | LIGHT-SENSITIVE CONSTRUCTS FOR INDUCING CELL DEATH AND CELL SIGNALING - A light-sensitive G-protein coupled receptor includes a light sensitive extracellular domain and a hetorologous intracellular domain capable of modulating an intracellular signaling pathway. | 01-28-2010 |
20100028967 | Method of changing property or function of substance and method of eliminating biofunction of cell - There are provided a method of changing a property or function of a substance by fragmenting a protein contained in the substance floating in a gas, and a method of eliminating a biofunction of a cell by applying to the cell either an electric discharge or a particle produced by the electric discharge, or both simultaneously, to fragment a protein contained in the cell. | 02-04-2010 |
20100028968 | BIOMECHANICAL TREATMENT FOR OBESITY AND DIABETES - Methods of maintaining or improving the metabolic state of a subject, e.g., a human, are disclosed. The methods can include providing to the subject a low magnitude, high frequency mechanical signal on a periodic basis and for a time sufficient to maintain or improve the subject's metabolic state. The subject can be diagnosed as having or can be at risk of developing, an obesity-related medical condition, e.g., type 2 diabetes, cardiovascular disease, hypertension, rheumatoid arthritis, and breast cancer. The methods can include a step of identifying a suitable subject by evaluating a physiological parameter that reflects the metabolic state of the subject, e.g., visceral fat content, subcutaneous fat content, body mass index, and blood pressure. | 02-04-2010 |
20100035321 | SYSTEMS, DEVICES, AND, METHODS FOR RELEASING BIOMASS CELL COMPONENTS - Systems, devices, and methods for releasing one or more cell components from a photosynthetic organism. A bioreactor system is operable for growing photosynthetic organisms. Some of the methods include contacting the photosynthetic organism with an energy-activatable sensitizer, and activating the energy-activatable sensitizer, thereby releasing a cellular component from at least one of, for example, a membrane structure, tubule, vesicle, cisterna, organelle, cell compartment, plastid, or mitochondrion, associated with the photosynthetic organisms. | 02-11-2010 |
20100041122 | CENTRIFUGAL FORCE-BASED SYSTEM FOR DETECTION/TREATMENT OF MEMBRANE-ENCASED STRUCTURES - Membrane-encased structures such as biological cells, liposomes, and vesicles, are conveyed through one or more channels in a rotating disk for individual exposure to optical elements or to electrodes, for purposes of transfection or flow cytometry. The rotation of the disk serves either to provide centrifugal force to urge the cells against one wall of the channel and in certain embodiments to move the cells through the channels, or to draw cells at preselected times or intervals into the exposure zone, or all three. | 02-18-2010 |
20100081185 | System and method for photodynamic cell therapy - The invention can be characterized as method for stimulating or inhibiting gene expression by photomodulating living cells using a source of narrowband multichromatic electromagnetic radiation. The cells may include, among others, nerve cells, skin cells, retinal cells, heart cells, stem cells, brain cells, cells found in human organs, cells found in hair follicles, and cells found in the human eye or retina. Photomodulation may be enhanced using topically or orally administered compositions. The source of narrowband multichromatic electromagnetic radiation may include at least one light emitting diode (LED) that can emit radiation having a wavelength of from about 300 nm to about 1600 nm. | 04-01-2010 |
20100093051 | LIGHT REGULATED TRANSCRIPTION SYSTEM FOR USE IN PROKARYOTIC ORGANISMS - A process for regulating transcriptional activity of a gene is provided. The process comprises introducing genetic material into an organism, placing a gene downstream of a light-regulated promoter within the organism having a light responsive element and subjecting the organism to an exogenous light source. The genetic material is selected from a photosynthetic bacterium and the light source is configured to control the level of transcriptional activity of the gene. | 04-15-2010 |
20100099159 | CAGED NUCLEOTIDES AND OLIGONUCLEOTIDES AND THEIR APPLICATION - There is disclosed nucleotides and nucleotide analogs having a protecting or “caging” group. There is further disclosed oligonucleotides and oligonucleotides analogs formed having a protecting or “caging” group. There is further disclosed a method for decaging the nucleotides and nucleotide analogs having a protecting or “caging” group and oligonucleotides and oligonucleotide analogs having a caging group. | 04-22-2010 |
20100105120 | PHOTOCLEAVABLE SENSE-ANTISENSE COMPLEX - There is disclosed a photocleavable sense-antisense nucleobase polymer complex capable of modulating gene expression comprising an unnatural antisense nucleobase polymer that targets an mRNA, and a photocleavable sense nucleobase polymer noncovalently bound to the antisense nucleobase polymer, wherein the photocleavable sense nucleobase polymer comprises a plurality of nucleobase polymers connected by a photocleavable linkage. There is also disclosed a method for controlling the time and spatial position of gene expression comprising selecting a target mRNA, introducing the photocleavable sense-antisense nucleobase polymer complex into a cell, and selectively irradiating the cell with light. | 04-29-2010 |
20100120113 | APPARATUS AND METHOD FOR MICROMANIPULATION OF MICROSCALE OBJECTS USING LASER LIGHT DELIVERED THROUGH A SINGLE OPTICAL FIBER AND AXICON LENS - A single optical fiber having a distal end is optically coupled to the laser and distilling terminated with an axicon lens optically coupled to the single optical fiber to form a microscopic distal tip to provide a spatially shaped elongated laser focused spot for microprocessing and/or microdissection of a microscale object. A pulsed or continuous laser beam or superposition of pulsed and continuous laser beams is generated, controllably spatially shaped, selectively oriented, selectively moved via movement of a single optical fiber terminated with the axicon lens, and the oriented, spatially shaped laser beam applied via the single optical fiber terminated with the axicon lens to a living or nonliving microscopic object for manipulation, micro-dissection, alteration/ablation, and excitation of the living or nonliving microscopic object. | 05-13-2010 |
20100124775 | PROCESS FOR TREATING CULTURED CELLS - According to the present invention, a process for treating cultured cells is provided, which comprises the steps of: a) providing planar, two-dimensional objects (“flakes”), wherein these objects comprise a material which, upon application of an extrinsic stimulus, is transferred from the planar state into a rolled state, b) providing cells on said flakes, and c) transferring the flakes from the planar state into a rolled state by application of said extrinsic stimulus. | 05-20-2010 |
20100136644 | METHODS FOR IMPROVING THE CULTIVATION OF AQUATIC ORGANISMS - The present invention relates to electrochemical methods for altering a biological characteristic of an aquatic organism such as an algae or bacterium. The invention includes alterations to biological characteristics such as growth rate, membrane permeability and buoyancy. | 06-03-2010 |
20100136645 | METHOD FOR PREPARING A CELL-DERIVED EXTRACELLULAR MATRIX SCAFFOLD - The present invention relates to a method for fabricating a cell-derived extracellular matrix scaffold, more particularly, to a method for fabricating a cell-derived extracellular matrix scaffold, the method comprising the steps of obtaining a chondrocyte/extracellular matrix (ECM) membrane from chondrocytes derived from animal cartilage, obtaining a pellet-type scaffold-free construct by culturing after centrifuging the obtained chondrocytes/extracellular matrix (ECM) membrane and freeze-drying the obtained pellet-type construct. The cell-derived ECM scaffold according to the invention is a porous scaffold fabricated using cartilage tissue engineered by culturing chondrocytes in an in vitro scaffold-free system, which is not reduced in size during the cultivation and thus useful for cartilage regeneration. | 06-03-2010 |
20100136646 | Selective Inactivation Of Microorganisms With A Femtosecond Laser - A method is provided for selectively inactivating microorganisms with femtosecond pulsed lasers. Under proper laser conditions, irradiation of the femtosecond pulsed laser causes inactivation of pathogenic microorganisms, for example, viruses, bacteria and protozoa, without causing cytotoxicity in mammalian cells. Pathogenic microorganism activity is diminished through an impulsive stimulated Raman scattering process, that is, through the excitation of the low-energy vibrational state on the outer structure of a microorganism with femtosecond pulsed lasers. The wavelength of the laser pulses is in a range of the electromagnetic spectrum, for example, visible and near-infrared where water is substantially transparent. The method is utilized for cleansing blood components, disinfecting drinking water, treating viral and bacterial diseases, extracting nucleic acid from microorganisms, and for manufacturing vaccines. | 06-03-2010 |
20100144002 | Methods for accelerating tissue processing - Systems and methods for accelerating tissue processing by treating tissue samples and one or more tissue processing agents with infrasonic vibrations are discussed. Some non-limiting examples of tissue processing agents include a tissue fixative, dehydrating agent, clearing agent, impregnating agent, embedding agent, tissue stain, enzyme, or another chemical that diffuses into the tissue sample when the sample is being preserved or prepared for microscopic examination. The infrasonic vibrations can have a frequency from about 10 to about 600 Hz. The infrasonic vibrations can have an amplitude that is sufficiently high, when combined with the frequency, to induce turbulent mixing of the processing agent and accelerate tissue processing. The tissue sample may optionally be vibrated with ultrasonic vibrations. The ultrasonic vibrations can have a frequency and amplitude that are sufficiently high to induce turbulent mixing of the processing agent and to accelerate tissue processing. | 06-10-2010 |
20100144003 | INDUCTION OF MICROBIAL SECONDARY METABOLITES - The present invention relates to the production of secondary metabolites from microorganisms. In particular, there are provided methods for inducing the rapid production of such compounds from a variety of microorganisms. | 06-10-2010 |
20100184182 | METHOD FOR PREPARING BIOLOGICAL TISSUE - This invention provides a means for preparing biological tissue within a short period of time. This invention provides a method for preparing biological tissue comprising: adding a culture solution containing cells and an extracellular matrix component that was separately prepared to a culture vessel having an inner bottom surface, which is a non-cell-adhesive surface; and performing cell culture while centrifugal or magnetic force toward the inner bottom surface is applied to cells to obtain biological tissue comprising multi-layered cells within a short period of time. | 07-22-2010 |
20100190228 | METHOD AND APPARATUS FOR FACILITATING EVALUATING MIGRATION OF CELLS IN VITRO - Method and apparatus for processing a cell culture are provided. The method includes establishing a cell culture within a holding device having one or more wells, each well holding a cell culture, and including a well substrate with at least one electrode in contact with the cell culture; periodically applying at least one electrical pulse to the at least one electrode to prevent cells from attaching to and achieving confluence over the at least one electrode while allowing cells to attach to and achieve confluence over other portions of the well substrate; and discontinuing the periodically applying of the at least one electrical pulse to the at least one electrode after cells have achieved confluence over the other portions of the well substrate, and thereafter, monitoring the cell culture to monitor migration of cells over the electrode(s) from the other portions of the well substrate. | 07-29-2010 |
20100190229 | SYSTEM FOR OPTICAL STIMULATION OF TARGET CELLS - Stimulation of target cells using light, e.g., in vivo, is implemented using a variety of methods and devices. In one example, embodiments involve methods for stimulating target cells using a photosensitive protein that allows the target cells to be stimulated in response to light. In another specific example embodiment, target cells are stimulated using an implantable arrangement. The arrangement includes an electrical light-generation means for generating light and a biological portion. The biological portion has a photosensitive bio-molecular arrangement that responds to the generated light by stimulating target cells in vivo. Other aspects and embodiments are directed to systems and methods for screening chemicals based screening chemicals to identify their effects on cell membrane ion channels and pumps, and to systems and methods for controlling an action potential of neuron (e.g., in vivo and in vitro environments). | 07-29-2010 |
20100203610 | METHODS OF TREATING CELL CULTURE MEDIA FOR USE IN A BIOREACTOR - The invention provides methods for treating cell culture media for use in a bioreactor using ultraviolet C (UVC) light and filtration. | 08-12-2010 |
20100209987 | Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses - The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material. | 08-19-2010 |
20100216206 | Method for Preparing an Acellular Organic Tissue for Revitalisation and Device for Implementing Said Method - The invention concerns a method for preparing an acellular organic tissue for revitalisation by means of the reimplantation of living cells, said method involving the following stages: preparing the acellular tissue on an essentially flat surface, creating a plurality of holes on the surface of the tissue, distributed all over said surface and positioned so that they penetrate at least through a portion of the thickness of said tissue. The holes are suitable for containing the living cells when they are reimplanted. The invention also includes a device for implementing said method, characterized in that it comprises—one or more needles ( | 08-26-2010 |
20100255555 | Optically induced cell lysis biochip - The present invention provides an optically induced cell lysis biochip, which comprises: an upper substrate made of a transparent, electrically conductive material; a lower substrate made of a transparent, electrically conductive material; a photoconductive layer formed under the lower surface of the upper substrate or on the upper surface of the lower substrate; and a chamber formed between the upper substrate and the lower substrate. When there is an electric potential difference between the upper and lower substrates, a light spot illuminated on the photoconductive layer can cause the impedance of the illuminated area to decrease, thereby causing the electric current to center on the illuminated area of the photoconductive layer. Further, the electric current flowing through the illuminated area can induce the cell lysis process of a target cell. | 10-07-2010 |
20100255556 | METHODS AND APPARATUS FOR MANIPULATION OF FLUIDIC SPECIES - The present disclosure relates generally to methods and apparatus for manipulating, detecting, imaging, and/or identifying particles, fluids, or other objects via electromagnetic fields, including methods and apparatus for identifying, sorting, splitting, coalescing, and/or reacting such particles, fluids, or other objects. Certain aspects of the invention are generally directe to methods and devices for producing electric or magnetic fields, e.g., from one or more field-generating components ( | 10-07-2010 |
20100267104 | METHOD FOR THE EFFECTIVE DELIVERY OF PHOTONIC ENERGY TO CULTURES IN A FLUID MEDIUM - A method of preventing bio-filming during the delivery of photonic energy to a culture or set of organisms in a fluid culture medium, the method including introducing a fluid energy-transfer medium into the fluid culture medium through an interface and distributing light into the fluid energy-transfer medium as it is introduced into the fluid culture medium. | 10-21-2010 |
20100267105 | Method for Micromanipulation of Cells - The present invention is a method for manipulating living cells in vitro using poly-2-hydroxyethylmethacrylate-coated surfaces and optical tweezers to obtain cells in isolation or to create specific cell-cell interactions. | 10-21-2010 |
20100267106 | METHOD AND CIRCUIT ARRANGEMENT FOR TREATING BIOMATERIAL - The invention relates to a method for treating biomaterial using at least one electrical field generated by a first voltage pulse which is terminated once the value for an electrical parameter has exceeded or dropped below a preset limit. After the first voltage pulse has been terminated, it is continued by an additional voltage pulse. The invention also relates to a circuit arrangement comprising at least one storage device for electrical charges to generate at least one voltage pulse by selectively discharging the storage device, and at least one control unit for controlling the discharge. The present invention provides a controller for monitoring the chronological progression of the voltage pulse, said controller controlling at least one continuation of discharge after termination. | 10-21-2010 |
20100279373 | DEVICE AND PROCESS FOR TREATING A LIQUID MEDIUM - A device for treating liquid medium has an ultrasound emitter located relative to a compartment of a container holding a liquid medium and a microbubble emitter located relative to the compartment. The ultrasound emitter emits high-frequency ultrasound between about 200 KHz and 10 MHz. The microbubble emitter emits bubbles with an average diameter of less than 1 mm. | 11-04-2010 |
20100291654 | PREPARATION FOR PHOTODYNAMIC CONTROL OF MICROORGANISMS AND USE THEREOF - The invention relates to a preparation for the photodynamic control of micro-organisms, said preparation being in a liquid or pasty form and containing a photosensitiser which comprises a dyestuff and produces singlet oxygen when irradiated by means of light. The micro-organisms can be marked by means of the dyestuff. The aim of the invention is to improve said preparation in order to enable an improved photodynamic control. To this end, the preparation contains an active ingredient for amplifying or weakening the oxidative action of the singulet oxygen by chemical manipulation of the dyestuff or the nanoenvironment thereof. In a particular form of embodiment, a rinsing solution is used before the irradiation. | 11-18-2010 |
20100317080 | METHOD FOR THE TREATMENT OF BIOLOGICAL TISSUE OF ANIMAL OR HUMAN ORIGIN, SUCH AS PORCINE, BOVINE PERICARDIUM OR HUMAN CADAVER HEART VALVES, AND BIOLOGICAL TISSUE TREATED ACCORDINGLY - A method for treating glutardialdehyde-stabilised biological tissue of animal or human origin, such as porcine, bovine pericardium or human cadaver heart valves, provides a physical plasma treatment of the, in particular, collagen tissue for increasing the biocompatibility, cell colonisation and durability thereof. | 12-16-2010 |
20100323419 | Methods and Devices for Charged Molecule Manipulation - Systems and methods for manipulating molecular material are provided. In one aspect, for example, a method for manipulating molecular material may include positioning an uncharged needle structure in electrical proximity with a charged molecular material at a first locus in a liquid environment, charging the needle structure such that at least a portion of the charged molecular material becomes associated with the needle structure, moving the needle structure and the first locus relative to one another, and discharging the needle structure to disassociate the charged molecular material at a second locus. | 12-23-2010 |
20100330644 | BOUNDARY CONDITIONS FOR THE ARRANGEMENT OF CELLS AND TISSUES - The present invention relates to the arrangement of one or more cells in a medium or on a substrate through the use of boundary conditions, which are changes in local environment compared to the medium or substrate alone or cause an alteration of cell response upon interaction of a cell with the boundary condition. | 12-30-2010 |
20110014673 | Systems and Methods for Effecting a Physical Change in a Biological Sample - The present invention relates generally to systems and methods for processing a biological sample that result in a physical change, such as reacting two molecules together to form a reaction product or for use in lysing viruses or biological cells for analysis using biological assay systems. As such, the present invention relates both to breaking apart biological species such as viruses and cells, as well as the formation of reactants from one or more reactive species. The sample has a volume in the range from about 1 microliter to 10 milliliters. The sample is processed by applying pressure, and either sonic energy or thermal energy to the sample, wherein the pressure achieved is usually at least 24 atmospheres, and the temperature of the sample is usually raised to at least 50° C. | 01-20-2011 |
20110027853 | Phantom for the experimental in-vitro validation of radiation procedures under the influence of motion, taking into account the biological effective dose - A phantom device for in-vitro validation of radiation procedures under motion influence in consideration of an effective biological dose includes a phantom having a first biological detector with a first biological sample. The first biological sample includes a plurality of culturing and irradiation elements. Each of the culturing and irradiation elements are provided with a respective biological sub-sample so that the first biological detector is configured as a spatially resolving biological detector. A first motion device is configured to move the first biological detector so as to simulate a motion of a target volume. | 02-03-2011 |
20110045565 | APPARATUS AND METHOD FOR ACCELERATED TISSUE INFILTRATION BY MEANS OF MICROWAVE EXCITATION - A tissue infiltration apparatus, system and method are described, allowing accelerating infiltration of the tissue sample with a variety of reagents fluids. The apparatus comprises a reaction chamber adapted to receive a tissue sample and a dielectric fluid to be infiltrated into a tissue sample. The reaction chamber comprises a bottom and a circumferential wall with a window section that is transparent for microwave irradiation. A holding element holds the tissue sample over a predetermined time period at a distance of 3PD of the dielectric fluid from the window section is provided. PD is the penetration depth of the microwaves into the dielectric fluid and defined as the depth where the initial microwave field intensity has been reduced to 1/e. A microwave system irradiates microwave irradiation through the window section into the reaction chamber. | 02-24-2011 |
20110045566 | METHOD FOR PREPARING THE DECELLULARIZED MATRIX - A method for preparing the decellularized matrix using the phospholipase includes the following steps: pretreating the standby tissue and organ; putting the standby tissue and organ into the solution containing the phospholipase; preparing the decellularized matrix in the control condition; washing the prepared decellularized matrix. | 02-24-2011 |
20110053240 | DNA DAMAGING AGENTS IN COMBINATION WITH TYROSINE KINASE INHIBITORS - The present invention relates to the signalling pathways connecting DNA damage, such as that induced by ionizing radiation or alkylating agents, and phosphorylation by tyrosine kinases. | 03-03-2011 |
20110053241 | DEVICE AND METHOD FOR MECHANICALLY DEFORMING CELLS - The present invention relates to a device for mechanically deforming cells comprising: a cell holding element ( | 03-03-2011 |
20110065161 | BIPOLAR SOLID STATE MARX GENERATOR - A high-voltage bipolar rectangular pulse generator using a high efficiency solid-state boosting front-end and an H-bridge output stage is described. The topology of the circuit generates rectangular pulses with fast rise time and allows easy step-up input voltage. In addition, the circuit is able to adjust positive or negative pulse width, dead-time between two pulses, and operating frequency. The intended application for such circuit is algae cell membrane rupture for oil extraction, although additional applications include biotechnology and plasma sciences medicine, and food industry. | 03-17-2011 |
20110076734 | Electrowetting Microarray Printing System and Methods for Bioactive Tissue Construct Manufacturing - Apparatuses and methods for manufacturing three-dimensional, bioactive, tissue scaffold fabrications with embedded cells and bioactive materials, such as growth factors, using biomimetic structure modeling, solid freeform fabrication, biocompatible hydrogel material, and electrowetting on dielectric-based multi-microarray printing. | 03-31-2011 |
20110086403 | PHOTOINHIBITION OF MICROBIAL NITRIFICATION IN POTABLE WATER - A system for the photo-inhibition of microbial nitrification of potable water may include overhead, floating or submerged lamps (fluorescent, incandescent, LED, etc. . . . ) with spectral emission between 315-430 nm, headspace and submerged light meters for measuring the intensity above and below the water surface, a water mixing device to manage full water column dose, and a controller for operating associated lamp and mixer operations. The applied irradiance may typically be equal to or exceed 0.01 W/m | 04-14-2011 |
20110104777 | Method of making an artificial micro-gland that is anisotropic - A method is disclosed for making an artificial micro-gland having a continuous anisotropic membrane of two or more types of living cells. A first step includes forming a carrier fluid in a microchannel in a laminar flow of two distinct fluid flows. Another step includes introducing a template, which may itself be anisotropic, into the microchannel in a manner such that the template straddles the interface between the first fluid-flow and the second fluid-flow. In some embodiments two types of living cells within the template are separately attracted one of the fluid flows by the presence of an agent of taxis. In other embodiments, cells within one or the other of the fluid flows are attracted to agents within the template. Membranes form on the template and join together to form a complete cellular membrane around a reservoir. | 05-05-2011 |
20110143413 | System And Method For Controlling Bacterial Cells With Weak Electric Currents - A system and method for treating bacterial cells with an electrochemical process, alone or in combination with antibiotics. Weak electric currents are used to effectively eliminate bacterial cells. The method may be adapted for novel therapies of chronic infections and strategies to control persistent biofouling. The system has broad spectrum applications in treating chronic and drug resistant infections, such as those caused by | 06-16-2011 |
20110159561 | KIT AND METHOD FOR THE CAPTURE OF TUMOR CELLS - The invention relates to a kit and method for the capture of tumor cells in a body fluid sample or a serum-containing sample. The kit and method of the invention can capture living tumor cells but not non-living tumor cells or cell fragments so that the tumor species can be further identified by further culture of the captured tumor cells. Also, the kit and method of the invention can readily identify whether a sample contains tumor cells and collect these tumor cells for further identification so that the presence of cancer and development of the metastasis and early relapse can be identified. | 06-30-2011 |
20110177576 | SHOCK WAVE CELL TREATMENT DEVICE AND METHOD TO ENHANCE CELL REPLICATION | 07-21-2011 |
20110250669 | METHOD AND APPARATUS FOR SELECTIVELY TARGETING SPECIFIC CELLS WITHIN A CELL POPULATION - This invention provides a method and apparatus for selectively identifying, and targeting with an energy beam, specific cells within a cell population, for the purpose of inducing a response in the targeted cells. Using the present invention, every detectable cell in a population can be identified and affected, without substantially affecting non-targeted cells within the mixture. | 10-13-2011 |
20110306107 | HARDENED MYCELIUM STRUCTURE AND METHOD - A method of preparing a mycelium component, including growing live mycelium from a fungal inoculum and a liquid aggregate. The live mycelium is cured to terminate further mycelium growth and form a mycelium structure. Moisture is applied to the mycelium structure such that the mycelium structure is nearly fully saturated. The mycelium structure is dried to form a hardened mycelium structure. | 12-15-2011 |
20110312056 | Plasma Membrane Isolation - The present invention relates to a population of monodisperse magnetic nanoparticles with a diameter between 1 and 100 nm which are coated with a layer with hydrophilic end groups. Herein the layer with hydrophilic end groups comprises an inner layer of monosaturated and/or monounsaturated fatty acids bound to said nanoparticles and bound to said fatty acids, an outer layer of a phospholipid conjugated to a monomethoxy polyethyleneglycol (PEG) comprising a hydrophilic end group, | 12-22-2011 |
20120009644 | Tissue Implants for Implantation and Methods for Preparing the Same - A method is provided for preparing a tissue implant for implantation. The method includes harvesting a tissue material from a human or an animal donor, treating the tissue material with water or a hypotonic solution to lyse native cells of the tissue material, treating the tissue material in a nuclease-containing solution which includes an antimicrobial, treating the tissue material with an alkaline alcohol solution, treating the tissue material with a chlorine dioxide treatment solution, and sterilizing the tissue material. | 01-12-2012 |
20120094352 | METHOD FOR CELL IDENTIFICATION AND CELL SORTING - The present invention relates to a method and to compounds useable in the method for analysis of cells for the pre-sence of an analyte and sorting the cells on the basis of the analyte. The compounds used in the method are optically detectable because of the content of a fluorochrome and contain a binder fraction which can specifically bind an analyte, in particular an oligonucleotide. | 04-19-2012 |
20120171744 | MATERIALS FOR MAGNETIZING CELLS AND MAGNETIC MANIPULATION - A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field. | 07-05-2012 |
20120171745 | Method for Selectively Damaging and Killing Tumor Cells and Apparatus Therefor - A method and an apparatus capable of selectively damaging and killing tumor cells includes a step of irradiating tumor cells with a UV pulse flash having continuous emission spectra ranging at least from 230 to 270 nm, outside a living body of a human or a living body of a non-human animal or in a living body of a non-human animal. The UV pulse flash may have an accumulated irradiation amount per unit area that is achieved at a distance of 8 cm from a light source having an integrated output of, for example, 90, 180-7100 or 14200 J. | 07-05-2012 |
20120184009 | CONTINUOUS CULTURE APPARATUS WITH MOBILE VESSEL, ALLOWING SELECTION OF FITTER CELL VARIANTS AND PRODUCING A CULTURE IN A CONTINUOUS MANNER - A method and device for growing plant, animal or stem cells in a continuous manner. | 07-19-2012 |
20120190094 | Photoreactive Regulator of Protein Function and Methods of Use Thereof - The present invention provides a synthetic regulator of protein function, which regulator is a light-sensitive regulator. The present invention further provides a light-regulated polypeptide that includes a subject synthetic regulator. Also provided are cells and membranes comprising a subject light-regulated polypeptide. The present invention further provides methods of modulating protein function, involving use of light. The present invention further provides methods of identifying agents that modulate protein function. | 07-26-2012 |
20120190095 | Composition For Treatment of Neurodegenerative Diseases or Disorders, Method and Use Comprising Electromagnetically Irradiated Yeast - Provided is a composition which comprises yeast cells treated with, or grown from yeast cells treated with electromagnetic waves in the range of 30 GHz to 300 GHz. Said composition may be used for the treatment of neurodegenerative diseases or disorders. A method relating to the composition is also provided. | 07-26-2012 |
20120214217 | IN SITU TISSUE ENGINEERING USING MAGNETICALLY GUIDED THREE DIMENSIONAL CELL PATTERNING - Methods are provided for the three dimensional manipulation of cells, and for the formation of an organized engineered cell tissue. Also provided are the organized engineered cell tissues produced by the methods. In one method, a plurality of magnetically labeled cells are mixed with a cross-linkable hydrogel to form a cell-hydrogel mixture, the at least a portion of the plurality of magnetically labeled cells are manipulated with a magnetic field to arrange the magnetically labeled cells into a specific cellular arrangement, and the hydrogel is crosslinked to form the organized engineered cell tissue. The approach presented herein offers a means to circumvent the deficiencies in the field of regenerative medicine, and allows for the production of organized tissues in situ with specific cellular organizations that mimic the native tissue. | 08-23-2012 |
20120264186 | METHOD FOR PREPARING ANIMAL CELLS CAPABLE OF PROLIFERATION - A method for preparing animal cells with good adhesion and proliferation properties which are capable of proliferation is provided. the method permits the detachment of cultured cells without damaging the cells. A method for preparing a sheet of animal cells such as skin cells which are capable of proliferation is also provided. A method for preparing animal cells capable of proliferation, comprising the steps of (1) culturing animal cells on a substrate at least one portion of which is an electrode, and (2) applying a high-frequency wave potential to the electrode to detach the cells that have adhered to the substrate surface through culturing. The high-frequency wave potential is of a frequency falling within a range of 1 KHz to 10 MHz with a potential falling within a range of ±1.0 V (vs. Ag/AgCl) or less. The culture medium during separation does not contain calcium or magnesium. | 10-18-2012 |
20120329121 | ENHANCING ALGAE GROWTH BY REDUCING COMPETING MICROORGANISMS IN A GROWTH MEDIUM - A method is described by which a growth medium is exposed to an electric field of sufficient magnitude to kill competing microorganisms and insufficient magnitude to cause flocculation to an algae population. | 12-27-2012 |
20130078701 | DEVICE COMPRISING A CONDUCTIVE SURFACE AND A CONDUCTIVE POLYMER FOR ADHESION OF CELLS AND TISSUE - The present disclosure relates to a device comprising a conductive substrate surface ( | 03-28-2013 |
20130164811 | DNA DAMAGING AGENTS IN COMBINATION WITH TYROSINE KINASE INHIBITORS - The present invention relates to the signalling pathways connecting DNA damage, such as that induced by ionizing radiation or alkylating agents, and phosphorylation by tyrosine kinases. | 06-27-2013 |
20130196404 | METHOD FOR DETACHMENT AND PREPARATION OF LIVING MICROORGANISMS - A method for immobilizing living microorganisms includes a step (1) of disposing a solution containing microorganisms as an electrolyte on the surface of a substrate at least one portion of which is an electrode, and applying a constant potential to the electrode to cause at least a portion of the microorganisms to attach to the surface of the substrate. The constant potential in step (1) is greater than −0.5 V but not greater than −0.2 V (vs Ag/AgCl) or greater than +0.2 V but not greater than +0.4 V (vs Ag/AgCl). The electrolyte in step (1) does not contain a source of nutrition for the microorganisms. | 08-01-2013 |
20130203145 | Continuous Flow Bioreactor for Magnetically Stabilized Three-Dimensional Tissue Culture - The invention provides methods for rapid, continuous generation of cells and cell products using magnetically stabilized three-dimensional tissue culture. The invention also pertains to a continuous flow self-regulating closed system bioreactor system for magnetically stabilized three-dimensional tissue culture. The methods described here do not use traditional solid scaffolding for cell culture. | 08-08-2013 |
20130288328 | VISIBLE LIGHT MODULATION OF MITOCHONDRIAL FUNCTION IN HYPOXIA AND DISEASE - The present invention provides methods of using electromagnetic radiation in the visible portion of the spectrum to modulate mitochondrial function in the treatment of various conditions, including Alzheimer's disease, other dementias, hypoxia and diabetic peripheral neuropathy, and sensory disorders of the extremities. | 10-31-2013 |
20130302872 | PRODUCTION OF CELL TISSUE HAVING THREE-DIMENSIONAL STRUCTURE USING ELECTROSTATIC INK JET PHENOMENON - A method and an apparatus for producing a cell tissue of blood vessels or the like with the use of an electrostatic ink jet phenomenon are provided. A pattern generator for cell tissue patterning on a substrate includes at least one vessel for holding a solution containing materials used for cell tissue formation, a substrate that is an object of patterning, and a pattern generator including a voltage applying unit for applying a voltage to the vessel. The voltage is applied to the vessel by the voltage applying unit to generate an electric field between the vessel and the substrate, causing the solution to be ejected from the vessel as a result of an electrostatic ink jet phenomenon and allowing the ejected solution to adhere to the substrate. | 11-14-2013 |
20130316429 | ANTI-APOPTOSIS OR ANTI-NECROSIS INDUCTION METHOD - Disclosed herein is a method for simply inducing an anti-apoptotic effect and/or an anti-necrotic effect in a living cell with good control without administering any drug. The method includes applying an alternating-current voltage to the living cell so that an electric current of 25 μA or higher but 75 μA or lower flows to induce an anti-apoptotic effect and/or an anti-necrotic effect in the living cell. The living cell used may be a cultured cell. The alternating-current voltage may be applied to a stage member on which a container holding the living cell is placed. | 11-28-2013 |
20130344559 | DEVICE AND METHOD FOR CONTROLLING NERVE GROWTH - A nerve growth chamber includes a stimulation vessel defined by one or more sidewalls; at least one anode and at least one cathode positioned in the sidewall or sidewalls of the stimulation vessel; a conductive medium in the stimulation vessel; nerve tissue in the stimulation vessel; and a signal generator connecting the at least one anode and the at least one cathode and activated to generate a periodic AC signal at a desired frequency and amplitude, the periodic AC signal traveling through the conductive medium and affecting the growth of the nerve tissue. A method in accordance with the operation of the apparatus is also provided. | 12-26-2013 |
20140087440 | MAGNETIC THREE-DIMENSIONAL CELL CULTURE APPARATUS AND METHOD - A culture apparatus and method for growing cells and tissue in a three-dimensional configuration harnesses magnetic, paramagnetic, ferromagnetic and diamagnetic forces. The cells or tissue are grown with magnetized core particles and are suspended via magnetic forces in a native, non-restricted, three-dimensional configuration while being maintained in a normal gravity (1 g) growth environment in the absence of rotational alteration of the gravity vector. | 03-27-2014 |
20140162338 | DEVICE AND METHOD FOR PRODUCING A COLD, HOMOGENEOUS PLASMA UNDER ATMOSPHERIC PRESSURE CONDITIONS - The invention relates to a special plasma source designated as a plasma intractor (PI) for producing a cold, homogeneous plasma under atmospheric pressure conditions, which plasma source can be used advantageously to excite and control reactive processes in flowing media. The device according to the invention is characterized in that the device comprises at least 6 elongated electrodes ( | 06-12-2014 |
20140212943 | METHOD FOR IN-VITRO TREATMENT OF DIFFERENTIATED OR UNDIFFERENTIATED CELLS BY APPLICATION ELECTROMAGNETIC FIELDS - A process is described for the treatment of stem cells or differentiated cells by application of radiofrequency electro-magnetic fields, by means of an electromagnetic field generator ( | 07-31-2014 |
20140234938 | HARMONIC GENERATION FOR ACTIVATION OF SPECIES AND/OR DELIVERY OF SPECIES TO A TARGET ENVIRONMENT - Systems and methods for the activation of species and/or the delivery of species to a target environment using harmonic generation materials are generally described. | 08-21-2014 |
20140242662 | Manipulation of Cells on a Droplet Actuator - A method of growing cells on a droplet actuator is provided. The method may include providing a droplet actuator including a cell culture droplet including a cell culture medium and one or more cells; and maintaining the droplet at a temperature suitable for causing the cells to grow in the cell culture medium on the droplet actuator. Related methods, droplet actuators, and systems are also provided. | 08-28-2014 |
20140256015 | CAGED COMPOUND WHICH CAN BE PHOTOACTIVATED SELECTIVELY FOR CELL TYPES - An objective of the present invention is to provide a caged compound that can be photoactivated selectively for specific target cell types and can be used in an individual organism. The objective can be achieved by a compound represented by general formula K-Q-X, which is prepared by binding bioactive substance X, photocleavable protecting group Q, and compound K, which can be an enzyme substrate and is dissociated from Q-X by an enzyme reaction, wherein: Q is a protecting group that is photocleaved by light with a specific wavelength and then dissociated from X, when K is not bound thereto; X is a substance that does not express bioactivity when Q is bound thereto, but expresses bioactivity when Q is dissociated therefrom; and K is dissociated from Q by the above enzyme, so as to form a compound represented by Q-X. Specifically, the objective can be achieved with the use of such a compound, which is characterized in that Q is dissociated from X when the compound represented by Q-X is photoirradiated, and thus K expresses bioactivity. | 09-11-2014 |
20140322784 | SYSTEMS AND METHODS FOR MAGNETIC GUIDANCE AND PATTERNING OF MATERIALS - Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly. | 10-30-2014 |
20140342427 | PHOTONIC NANOANTENNA MEDIATED GENE CIRCUIT RECONFIGURATION - A selectively addressable optical biomolecular carrier and its method of use for reconfiguring gene circuits are described. One carrier is a plasmon resonant nanoantenna formed from a gold metal nanorod coated with a cationic phospholipid bilayer with an aspect ratio between 2.0 and 8.0 and plasmon resonance wavelength in the near infrared range. Biomolecules such as siRNA adhere to the carrier and are introduced into a cell. The biomolecules are released from the nanoantenna carriers with exposure to light at the plasmon resonance wavelength. The nanoantenna efficiently converts absorbed optical energy to surface localized heat releasing the biomolecules at a time determined by the user. The carrier can be used to modify gene circuits by allowing temporal control over the genes within a selected gene circuit through the optical release of interfering nucleotides. Optical silencing of endogenous genes with siRNA released from nanoantenna carriers was used to illustrate the methods. | 11-20-2014 |
20140377831 | METHOD AND DEVICE FOR ENHANCING A DIRECTIONAL MIGRATION OF STEM CELLS - The invention provides a method of enhancing a directional migration of stem cells, comprising providing one or more stem cell(s) and irradiating the stem cells with an effective energy of green light thereby enhancing stem cell directionally migrating, wherein the orientation of migration of stem cells is opposite to the green light source. Also provided is a stem cell treated by the method of the invention and a device for enhancing a directional migration of stem cells. | 12-25-2014 |
20140377832 | INDUCTION OF DEDIFFERENTIATION OF MESENCHYMAL STROMAL CELLS - The invention relates to induction of reprogramming of somatic cells, by methods which require mild growth conditions. Disclosed are methods of inducing dedifferentiation of mesenchymal stromal cell (MSC), by seeding or incubating mesenchymal stromal cells (MSCs) at low density, and without introduction or expression of exogenous genes in the cells. | 12-25-2014 |
20150037863 | METHOD OF FORMING A MULTILAYER AGGREGATE OF OBJECTS - A method of forming a multilayer aggregate of objects in a channel including a liquid, the method including: a) providing objects at first and second superposed regions of the channel, b) obtaining first and second aggregates of objects, optionally by applying transverse acoustic waves, preferably stationary waves, within each region to objects, and c) bringing the first and second aggregates into contact to form the multilayer aggregate of objects by submitting the first and second aggregates to: gravity in absence of acoustic waves, or to acoustic waves, optionally stationary waves, inducing displacement of the first and second aggregates toward each other. | 02-05-2015 |
20150056678 | DISEASE THERAPY USING DYING OR DEAD CELLS - A method of treating a disease characterized by a pathological immune response in a subject in need thereof is disclosed. The method comprises administering to the subject a therapeutically effective amount of a cell preparation which comprises dying or dead leukocytes, the dying or dead leukocytes being capable of suppressing the pathological immune response, thereby treating the disease in the subject. | 02-26-2015 |
20150072394 | Opsin Polypeptides and Methods of Use Thereof - The present disclosure provides opsins, including variant opsins with increased activity and/or increased trafficking to the plasma membrane. The opsins are useful in therapeutic and screening applications, which are also provided. | 03-12-2015 |
20150104844 | MATERIALS FOR MAGNETIZING CELLS AND MAGNETIC MANIPULATION - A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field. | 04-16-2015 |
20150361388 | ULTRASONIC APPARATUS AND METHOD FOR CELL RELEASE - An apparatus ( | 12-17-2015 |
20150368633 | Pharmaceutical Composition for Elevating Radiation-Sensitivity of Cancer Cells, Pharmaceutical Composition for Detecting Cancer Cells with Radiation-Sensitivity, and Detection Method Thereof - The present invention relates to a pharmaceutical composition for elevating radiation-sensitivity of cancer cells, which comprises: a nanoparticle containing with a first element, which is iron, copper, or the combination thereof; and a pharmaceutically acceptable carrier, wherein the nanoparticle is a metal nanoparticle, an alloy nanoparticle, or a metal nanoparticle with core-shell structure. In addition, the present invention provides a detection method to detect radiation-sensitivity of the cancer cells through different modalities such as CT or MRI due to its native high CT number and magnetic property. Furthermore, the present invention provides a pharmaceutical composition for elevating radiation-sensitivity of the cancer cells through preferential uptake of the nanoparticle, in order to enhance the radiation-sensitivity of the cancer cells and improve the efficiency of radiation therapy to the cancer cells. | 12-24-2015 |
20160002620 | METHOD FOR DIGITAL TRANSDUCTION OF DNA IN LIVING CELLS - A system and method for inducing cytotoxicity, comprising a receiver configured to receive an electromagnetic signal from a container, using a receiver configured to capture electromagnetic emissions from the container over a frequency range of at least 100 Hz to 10,000 Hz; an amplifier configured to amplify the received electromagnetic signal; and an emitter configured to emit the amplified electromagnetic signal in proximity to living cells. DNA from a pathogen is amplified using PCR, purified, and serially diluted. Electromagnetic signals from the diluted DNA are received, and optionally stored. The receive signal is amplified and emitted in proximity to living cells, to produce under selected circumstances, a cytopathic effect. | 01-07-2016 |
20160030637 | USE OF MICROPARTICLES AND ENDOTHELIAL CELLS WITH DECELLULARIZED ORGANS AND TISSUES - The invention provides a method for maintaining capillary lumen diameter, reducing a decrease in capillary vessel lumen diameter or expanding capillary vessel lumen diameter in a re-endothelialized decellularized organ or tissue graft with an intact extracellular matrix vascular network. The method is based on administration of endothelial cells and microparticles to the decellularized ECM. | 02-04-2016 |
20160032271 | ENCAPSULATION OF BACTERIA AND VIRUSES IN ELECTROSPUN FIBERS - A method of preserving organisms in viable form, the method comprising: suspending organisms in a solution of electrospinnable polymer; drawing droplets of said solution through a spinneret; applying an electrostatic field to said droplets under electrospinning conditions; so as to form fibers having a diameter no greater than about 5 μm within which distinct organisms are encapsulated in viable form. | 02-04-2016 |
20160053214 | BIOREACTOR, DEVICES, SYSTEMS AND METHODS - Disclosed are bioreactor devices, systems and methods. A bioreactor system can include one or more bioreactor modules that can be individually controllable and identifiable. A bioreactor module can be connected to one or more functional modules such as a pump module, a stimulation signal generation module, a motor module, a mechanical transmission module, a gas exchange module, a temperature module, a humidity module and/or a CO | 02-25-2016 |
20160058728 | CANCER STARVATION THERAPY - The present invention is a glutamine compound having a high Z element attached via a ligand, which enters the mitochondrion and is subsequently exposed to ionizing radiation. When exposed to ionizing radiation, the present invention damages mitochondrial (as well as other) substructures such as mtDNA, the outer membrane, the inner membrane, cristae, ribosomes, etc., and causes the effective destruction of such mitochondrion. Tumorigenic cells without mitochondria cannot produce the energy they need to subsist and replicate, effectively starving them of energy and causing their destruction. | 03-03-2016 |
20160083692 | ELECTROACTIVE POLYMERIC SCAFFOLDS AND METHOD FOR DELIVERING NERVE GROWTH FACTOR TO NERVE TISSUE - A polymerizable unit that yields an electrochemically responsive polymer (advantageously pyrrole) is anchored by polymerization within a polycaprolactone matrix to form an electroactive scaffold upon which cells can be cultured and in which the micro- and nano-topological features of the polycaprolactone matrix are preserved. A scaffold manufactured in accordance with the preferred embodiment can support Schwann cells, which produce nerve growth factor when electrically stimulated. Nerve growth factor has been demonstrated to promote the regeneration of nerve tissue. By implanting the scaffold on which Schwann cells have been cultured into damaged nerve tissue and applying a voltage across the scaffold, nerve growth factor is produced, thereby promoting repair of the damaged nerve tissue. | 03-24-2016 |
20160137974 | MICROPLATES FOR MAGNETIC 3D CULTURE - Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates and adjustable height cap for same. Similar devices for multi-magnet culture plates wherein multiwell plates have all adjacent magnets orientated in the opposite polarity, and methods of making same. | 05-19-2016 |
20160139069 | LARGE SCALE, LOW COST NANOSENSOR, NANO-NEEDLE, AND NANOPUMP ARRAYS - A nanoscale probe includes a substrate and a pair of nanoscale wires each having a first end disposed on the substrate and a second end. The second ends of each nanoscale wire are in contact with one another such that the pair of nanoscale wires form a bridge extending over the substrate. The nanoscale wires may be electrically connected to electrodes residing on the substrate. The electrodes, in turn, are connected to an active electronic device such as a readout device or microprocessor formed in the substrate on which the probe is located. In this way a property of the nanoscale wires, and thus of the cell, may be determined. | 05-19-2016 |
20160145566 | RECONFIGURABLE ASSEMBLY WITH FARADAY WAVE-BASED TEMPLATES - A method manufactures a structure based on reconfigurable assembly with faraday wave-based templates. The method includes the steps of providing a chamber containing a gas-liquid interface or liquid-liquid interface and dispersing a plurality of floaters at the gas-liquid interface or liquid-liquid interface. The method further includes oscillating the chamber along an axis orthogonal to the gas-liquid interface or liquid-liquid interface, thereby generating a standing wave formed by a parametric instability on the surface of the liquid. After formation of the standing wave, the floaters are allowed to self-assemble, at which point the floaters can be linked together. | 05-26-2016 |
20160159821 | NOVEL COPPER-CYSTEAMINE AND METHODS OF USE - Structure and luminescence properties of a new Cu-Cyteamine (Cu-Cy) crystal material are provided. The crystal structure of the Cu-Cy is determined by single crystal X-ray diffraction. It is found that the compound crystallizes in the monoclinic space group C2/c and cell parameters are a=7.5510(4) Å, b=16.9848(7) Å, c=7.8364(4) Å, β=104.798(3)°. The new Cu-Cy crystal material of the invention is also useful for treatment of cancer. | 06-09-2016 |
20190144843 | CONCENTRATION AND WASHING OF PARTICLES WITH ACOUSTICS | 05-16-2019 |