Class / Patent application number | Description | Number of patent applications / Date published |
435468000 | Introduction of a polynucleotide molecule into or rearrangement of a nucleic acid within a plant cell | 51 |
20080241931 | System For Expression of Genes In Plants - The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants. | 10-02-2008 |
20080311661 | RIBONUCLEIC ACID INTERFERENCE MOLECULES OF ARABIDOPSIS THALIANA - Sequences of ribonucleic acid interference molecules are provided. For example, in one aspect, at least one nucleic acid molecule comprising at least one of one or more precursor sequences having SEQ_ID NO: 1 through SEQ_ID NO: 3,197 and one or more corresponding mature sequences having SEQ_ID NO: 3,198 through SEQ_ID NO: 6,565 is provided. Techniques are also provided for regulating gene expression. | 12-18-2008 |
20090011510 | Device and method for biolistic transformation of cells - The present invention provides a device and method for biolistic bombardment of cells. In a preferred embodiment, the device comprises a hollow tube or cylinder that attaches to a standard biolistic transformation apparatus, and focuses the nucleic acids at cells and tissue sections. In a typical configuration, the device has two barrels that deliver equal or substantially equal amounts of nucleic acids to cells of a tissue, such as a plant leaf. | 01-08-2009 |
20090042300 | METHOD FOR ENHANCING GENE EXPRESSION IN PLANTS - The invention relates to the field of gene expression in plants and describes methods and constructs using the first intron of a FAD2 gene in order to enhance gene expression. | 02-12-2009 |
20090093059 | COMPOSITIONS AND METHODS TO STACK MULTIPLE NUCLEOTIDE SEQUENCES OF INTEREST IN THE GENOME OF A PLANT - Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome. | 04-09-2009 |
20090111186 | PROMOTER AND VECTORS FOR PLANT TRANSFORMATION AND METHODS OF USING SAME - The invention is directed to a promoter, designated MuB, sequences which hybridize to same and functional fragments thereof. The regulatory element of the invention provide improved expression in plants of operably linked nucleotide sequences. Expression vectors with the regulatory element is the subject of the invention, which may further include an operably linked nucleotide sequence. The invention is further directed to transformed plant tissue including the nucleotide sequence and to transformed plants and seeds thereof. The regulatory element is useful for driving gene or antisense expression or the like for the purpose of imparting agronomically useful traits such as, but not limited to, increase in yield, disease resistance, insect resistance, herbicide tolerance, drought tolerance and salt tolerance in plants. | 04-30-2009 |
20090111187 | Moss Expressing Promotion Regions - Disclosed are isolated nucleic acid molecules encoding wild type nucleus derived moss expression promoting regions (MEPRs) as well as a method for producing recombinant polypeptides using such MEPRs. | 04-30-2009 |
20090111188 | Optimized non-canonical zinc finger proteins - Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described. | 04-30-2009 |
20090155911 | Genetically modified plants and their applications in phytoremediation - Genetically modified plants able to accumulate heavy metals in shoots and methods of removing and possibly recovering said heavy metals, using said genetically modified plants. Said genetically modified plants include more than one copy of at least a sequence encoding a P1B-type ATPase of the Zn<2+>/Co<2+>/Cd<2+>/Pb<2+> subclass and that they overexpress said P1B-type ATPase. | 06-18-2009 |
20090197337 | Methods for the production of multimeric proteins and related compositions - Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions. | 08-06-2009 |
20090203142 | NUCLEOTIDE SEQUENCES OF A NEW CLASS OF DIVERGED DELTA-9 STEAROYL-ACP DESATURASE GENES - An isolated nucleic acid fragment encoding a diverged delta-9 fatty acid desaturase is disclosed. Also the construction of a chimeric gene encoding all or a portion of the diverged delta-9 fatty acid desaturase is disclosed, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the diverged delta-9 fatty acid desaturase in a transformed host cell. | 08-13-2009 |
20100105141 | Sulfonylurea-Responsive Repressor Proteins - Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell. | 04-29-2010 |
20100159598 | In Vitro Methods for the Induction and Maintenance of Plant Cell Lines as Single Suspension Cells With Intact Cell Walls, and Transformation Thereof - The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms. | 06-24-2010 |
20100248372 | Composition and Method for Modulating Plant Transformation - A plant culture medium composition for modulating plant transformation events, comprising a plant culture medium and an effective amount of at least one compound having a chloride component intermixed thereinto. In one embodiment, the at least one chloride-containing compound is selected from the group comprising: NaCl, MgCl | 09-30-2010 |
20100248373 | METHOD FOR INTRODUCING FOREIGN SUBSTANCE INTO CELL HAVING CELL WALL - Disclosed is a method for introducing a foreign substance ( | 09-30-2010 |
20100285591 | CYCLIN D POLYNUCLEOTIDES, POLYPEPTIDES AND USES THEREOF - The invention provides isolated polynucleotides, specifically Cyclin D polynucleotides, and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants for the purpose of increasing transformation efficiency. | 11-11-2010 |
20100291684 | MUTAGENESIS METHOD USING POLYETHYLENE GLYCOL MEDIATED INTRODUCTION OF MUTAGENIC NUCLEOBASES INTO PLANT PROTOPLASTS - Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis. | 11-18-2010 |
20110033937 | DOWN-REGULATION OF GENE EXPRESSION USING ARTIFICIAL MICRORNAS - Isolated nucleic acid fragments comprising precursor miRNA, and artificial miRNAs and their use in down-regulating gene expression are described. | 02-10-2011 |
20110039340 | COMPOSITIONS AND RELATED METHODS FOR MODULATING TRANSCRIPTIONAL ACTIVATION BY INCORPORATING GAG MOTIFS UPSTREAM OF CORE PROMOTER ELEMENTS - Compositions and methods for genetically modifying the production levels of nicotine and other alkaloids in plants are provided. An expression vector which comprises a tripartite GAG motif is also disclosed. | 02-17-2011 |
20110091975 | DOWN-REGULATION OF GENE EXPRESSION USING ARTIFICIAL MICRORNAS - Isolated nucleic acid fragments comprising precursor miRNAs, and artificial miRNAs and their use in down-regulating gene expression are described. | 04-21-2011 |
20110091976 | Regulatory region preferentially expressing to seed embryo and method of using same | 04-21-2011 |
20110312095 | METHOD AND CONSTRUCTS FOR INCREASING RECOMBINANT PROTEIN PRODUCTION IN PLANTS DEHYDRATION STRESS - The present invention provides methods and constructs for increasing recombinant protein production in plants during dehydration stress. The invention includes nucleic acids, inducible expression systems, and methods for using same for increasing recombinant protein production in plants and plant protection. | 12-22-2011 |
20120070900 | T-DNA/PROTEIN NANO-COMPLEXES FOR PLANT TRANSFORMATION - A DNA/protein nano-complex for transfecting eukaryotic cells, wherein the nano-complex comprises an expressible nucleotide molecule complexed with a VirD2 protein molecule, and a RecA protein molecule complexed with the nucleotide molecule/VirD2 protein molecule complex. The nucleotide molecule/VirD2 protein molecule/RecA protein molecule complexed may be further complexed to a cell penetrating peptide. The DNA/protein nano-complex can be used for delivering the expressible nucleotide molecule to target plant cells, in particular, monocot plant cells exemplified by wheat cells, corn cells, triticale cells, barley cells, and rye cells. | 03-22-2012 |
20120094385 | GENE POSITIONING SYSTEM FOR PLASTIDIC TRANSFORMATION AND PRODUCTS THEREOF - The present invention is directed to Gene Positioning technology for biosynthesis of one or more products of interest via plastid transformation of plants or algae, such as for example tobacco, Lemna, | 04-19-2012 |
20120107939 | DOWN-REGULATION OF GENE EXPRESSION USING ARTIFICIAL MICRORNAS - Isolated nucleic acid fragments comprising precursor miRNA, and artificial miRNAs and their use in down-regulating gene expression are described. | 05-03-2012 |
20120115234 | DOWN-REGULATION OF GENE EXPRESSION USING ARTIFICIAL MICRORNAS - Isolated nucleic acid fragments comprising precursor miRNA, and artificial miRNAs and their use in down-regulating gene expression are described. | 05-10-2012 |
20120282699 | MUTAGENESIS METHOD USING POLYETHYLENE GLYCOL MEDIATED INTRODUCTION OF MUTAGENIC NUCLEOBASES INTO PLANT PROTOPLASTS - Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis. | 11-08-2012 |
20120309092 | Angiogenin Expression in Plants - The present invention relates to plant-produced angiogenins, to related plant cells, plant calli, plants, seeds and other plant parts and products derived therefrom and to uses of plant-produced angiogenins. The present invention also relates to expression of angiogenin genes in plants and to related nucleic acids, constructs and methods. | 12-06-2012 |
20120329159 | Down-Regulation Of Gene Expression Using Artificial MicroRNAs - Isolated nucleic acid fragments comprising precursor miRNA, and artificial miRNAs and their use in down-regulating gene expression are described. | 12-27-2012 |
20130029425 | Genetic Inhibition by Double-Stranded RNA - A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods. | 01-31-2013 |
20130210151 | ENDONUCLEASE FOR GENOME EDITING - A chimeric endonuclease is provided comprising the GIY-YIG nuclease domain which is linked to a DNA-targeting domain by a linking domain. The endonuclease is useful in gene editing. | 08-15-2013 |
20140106457 | Stably maintained multiple copies of at least two ORF in the same orientation - The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of:
| 04-17-2014 |
20140154806 | TETRASELMIS PROMOTERS AND TERMINATORS FOR USE IN EUKARYOTIC CELLS - The present invention provides novel promoter and terminator sequences for use in gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the gene; and methods for stably transforming eukaryotic algae such as | 06-05-2014 |
20140193915 | COMPOSITIONS AND METHODS FOR CUSTOM SITE-SPECIFIC DNA RECOMBINASES - The invention relates to biotechnology and provides novel compositions and methods for sequence-specific or sequence-directed transcription activator-like effector recombinases. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, and molecular breeding are also provided. | 07-10-2014 |
20140234975 | METHOD FOR INCREASING THE EFFICIENCY OF DOUBLE-STRAND-BREAK INDUCED MUTAGENESIS - The present invention relates to a method for increasing double-strand-break induced mutagenesis at a genomic locus of interest in a cell, thereby giving new tools for genome engineering, including therapeutic applications and cell line engineering. More specifically, the present invention concerns the combined use of TALEN or meganucleases with TREX2, especially under the form of single-chain proteins. | 08-21-2014 |
20140315312 | MODIFIED GENE SILENCING - This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element. Another aspect includes providing a eukaryotic cell having a desired phenotype resulting from transcription in the eukaryotic cell of a modified suppression element. Also provided are transgenic eukaryotic cells, transgenic plant cells, plants, and seeds containing modified suppression elements, and useful derivatives of such transgenic plant cells, plants, or seeds, such as food or feed products. | 10-23-2014 |
20140356961 | USE OF PHASED SMALL RNAS FOR SUPPRESSION OF PLANT DEFENSE AND OTHER PLANT GENES - A method for generating a phased siRNA (phasiRNA) in a plant cell comprises introducing into the plant cell an effective amount of a microRNA (miRNA). A transcript of a NB-LRR encoding gene may be cleaved in the plant cell. Resistance of the plant to a microbe may be reduced. The plant may enter a symbiotic interaction with a microbe. Nodulation in the plant may be improved. Related compositions are provided. | 12-04-2014 |
20140370603 | TRANSGENIC CELLS WITH INCREASED PLASTOQUINONE LEVELS AND METHODS OF USE - Disclosed herein are transgenic cells expressing a heterologous nucleic acid encoding a prephenate dehydrogenase (PDH) protein, a heterologous nucleic acid encoding a homogentisate solanesyl transferase (HST) protein, a heterologous nucleic acid encoding a deoxyxylulose phosphate synthase (DXS) protein, or a combination of two or more thereof. In particular examples, the disclosed transgenic cells have increased plastoquinone levels. Also disclosed are methods of increasing cell growth rates or production of biomass by cultivating transgenic cells expressing a heterologous nucleic acid encoding a PDH protein, a heterologous nucleic acid encoding an HST protein, a heterologous nucleic acid encoding a DXS protein, or a combination of two or more thereof under conditions sufficient to produce cell growth or biomass. | 12-18-2014 |
20150037893 | PATHOGEN RESISTANT PLANT CELLS AND METHODS OF MAKING - The present invention relates to genetically modified plant cells that have reduced expression or activity of at least one amino acid efflux transporter and/or at least one mineral efflux transporter compared to levels of expression or activity of the at least one amino acid efflux transporter or mineral efflux transporter in an unmodified plant cell. The present invention also relates to genetically modified plant cells that have increased expression or activity of at least one amino acid influx transporter and/or at least one mineral influx transporter compared to levels of expression or activity of the at least one amino acid influx transporter or mineral influx transporter in an unmodified plant cell. | 02-05-2015 |
20150064790 | ENGINEERED ZINC FINGER PROTEINS TARGETING 5-ENOLPYRUVYL SHIKIMATE-3-PHOSPHATE SYNTHASE GENES - The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes. | 03-05-2015 |
20150087069 | Down-Regulation Of Gene Expression Using Artificial MicroRNAs - Isolated nucleic acid fragments comprising precursor miRNAs, and artificial miRNAs and their use in down-regulating gene expression are described. | 03-26-2015 |
20160152980 | METHOD OF REGULATING GENE EXPRESSION | 06-02-2016 |
20180023062 | COMPOSITIONS AND METHODS FOR CUSTOM SITE-SPECIFIC DNA RECOMBINASES | 01-25-2018 |
435469000 | Introduction via Agrobacterium | 6 |
20090191636 | Method of Producing Transgenic Graminaceous Cells and Plants - The present invention provides a method for producing a transgenic graminaceous plant cell, said method comprising: (i) obtaining embryonic cells from a mature graminaceous grain; and (ii) contacting said embryonic cells with a bacterium capable of transforming a plant cell, said bacterium comprising transfer-nucleic acid to be introduced into the embryonic cells, said contacting being for a time and under conditions sufficient for said bacterium to introduce said transfer-nucleic acid into one or more of the embryonic cells, thereby producing a transgenic graminaceous plant cell. The present invention also provides a method for producing a transgenic graminaceous plant. The present invention also provides a transgenic graminaceous plant cell and/or a transgenic graminaceous plant produced by said method. The present invention also provides a method for expressing a nucleic acid in a transgenic graminaceous plant cell or a transgenic graminaceous plant. | 07-30-2009 |
20090263902 | METHOD FOR REGULATING AGROBACTERIUM-MEDIATED TRANSFORMATION - A method for regulating expression of a virulence gene of | 10-22-2009 |
20130157369 | METHOD FOR IMPROVED TRANSFORMATION USING AGROBACTERIUM - Methods to increase transformation frequency in plants when using | 06-20-2013 |
20140273235 | ENGINEERING PLANT GENOMES USING CRISPR/Cas SYSTEMS - Materials and methods for gene targeting using Clustered Regularly Interspersed Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) systems are provided herein. | 09-18-2014 |
20160130595 | SYNTHETIC BI-DIRECTIONAL PLANT PROMOTER - This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a | 05-12-2016 |
20160376603 | METHOD OF TRANSFORMING CELLS - Use of an isolated | 12-29-2016 |
435470000 | Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection | 2 |
20100003759 | TARGETED DNA INSERTION IN PLANTS - Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving “double stranded break” including enzymes. Also provided are improved I-SceI encoding nucleotide sequences. | 01-07-2010 |
20140242703 | METHODS FOR TRANSFERRING MOLECULAR SUBSTANCES INTO PLANT CELLS - Provided are methods for introducing a molecule of interest into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. | 08-28-2014 |