Entries |
Document | Title | Date |
20080206827 | Method for producing chiral a-hydroxycarboxylic crystalline acids - The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of | 08-28-2008 |
20080213846 | Method of production of para-hydroxycinnamic acid using a thermostable TAL enzyme - A thermostable TAL enzyme was identified from the fungus | 09-04-2008 |
20080241899 | Poly (3-Hydroxyalkanoate) Block Copolymer Having Shape Memory Effect - Provided is a PHB block copolymer having orientation-induced rubber-elasticity and temperature-sensitive shape memory effects, comprising a plurality of 3-hydroxybutyrate (3HB) blocks as a repeating unit and a plurality of 3-hydroxyvalerate (3HV) blocks as a repeating unit, and optionally a hydroxy acid repeating group containing 6 or more carbon atoms. | 10-02-2008 |
20080311636 | Method for Producing Optically Active Alpha-Hydroxycarboxylic Acid - An efficient method for producing an optically active α-hydroxycarboxylic acid represented by the following general formula (I) [A represents a residue of a 5- or 6-membered cyclic compound, * indicates a carbon atom in the S- or R-configuration, X represents hydrogen atom or an alkyl group having 1 to 4 carbon atoms], which comprises the step of treating a corresponding ester compound (not optically pure) with cell bodies or a culture, or a processed product or an extract thereof of a microorganism of the genus | 12-18-2008 |
20090004707 | Process for Producing Glycolic Acid from Formaldehyde And Hydrogen Cyanide - A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from | 01-01-2009 |
20090004708 | Process For Producing Glycolic Acid From Formaldehyde And Hydrogen Cyanide - A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from | 01-01-2009 |
20090004709 | Process For Producing Glycolic Acid From Formaldehyde and Hydrogen Cyanide - A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from | 01-01-2009 |
20090004710 | Process For Producing Glycolic Acid From Formaldehyde And Hydrogen Cyanide - A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from | 01-01-2009 |
20090004711 | Process For Producing Glycolic Acid From Formaldehyde And Hydrogen Cyanide - A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from | 01-01-2009 |
20090011482 | Process For Producing Glycolic Acid From Formaldehyde And Hydrogen Cyanide - A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from | 01-08-2009 |
20090011483 | Process For Producing Glycolic Acid From Formaldehyde And Hydrogen Cyanide - A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from | 01-08-2009 |
20090221045 | METHOD FOR PRODUCING HYDROXYCARBOXYLIC ACID BY ENHANCING SYNTHESIS OF COENZYME - Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase. | 09-03-2009 |
20090233339 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 09-17-2009 |
20090305368 | METHOD FOR PRODUCING HYDROXYCARBOXYLIC ACID BY REGENERATING COENZYME - Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to regenerate oxidized-type nicotinamide adenine dinucleotide by being provided with an enhanced NADH dehydrogenase function by introducing a gene encoding NADH dehydrogenase into a microorganism. | 12-10-2009 |
20090325249 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 12-31-2009 |
20090325250 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 12-31-2009 |
20100015675 | Process for the production of fluorine containing alpha-hydroxy carboxylic acids - A process for the production of a fluorine containing α-hydroxy carboxylic acid by hydrolyzing a fluorine containing α-hydroxy nitrite in the presence of a nitrilase. | 01-21-2010 |
20100021978 | METHODS AND ORGANISMS FOR PRODUCTION OF 3-HYDROXYPROPIONIC ACID - A non-naturally occurring microbial organism having a 3-hydroxypropanoic acid (3-HP) pathway includes at least one exogenous nucleic acid encoding 3-HP pathway enzyme expressed in a sufficient amount to produce 3-HP. The 3-HP pathway includes a 2-keto acid decarboxylase, a CoA-dependent oxaloacetate dehydrogenase, or a malate decarboxylase. A method for producing 3-HP includes culturing a non-naturally occurring microbial organism having a 3-HP pathway that includes at least one exogenous nucleic acid encoding a 3-HP pathway enzyme expressed in a sufficient amount to produce 3-HP under conditions and for a sufficient period of time to produce 3-HP. The 3-HP pathway includes a 2-keto acid decarboxylase, a CoA-dependent oxaloacetate dehydrogenase, or a malate decarboxylase. | 01-28-2010 |
20100035314 | METHOD FOR THE ENZYMATIC PRODUCTION OF 2-HYDROXY-2-METHYL CARBOXYLIC ACIDS - The present invention relates to a method for the enzymatic production of 2-hydroxy-2-methyl carboxylic acids from 3-hydroxy carboxylic acids, where a 3-hydroxy carboxylic acid is produced in an aqueous reaction solution and/or is added to this reaction solution and is incubated. The aqueous reaction solution comprises a unit having 3-hydroxy-carboxylate-CoA mutase activity which has both 3-hydroxy-carbonyl-CoA ester-producing and 3-hydroxy-carbonyl-CoA ester-isomerizing activity and converts the 3-hydroxy carboxylic acid into the corresponding 2-hydroxy-2-methyl carboxylic acid which is isolated as acid or in the form of its salts. In a preferred embodiment, the unit having 3-hydroxy-carboxylate-CoA mutase activity is a unit which includes an isolated cobalamin-dependent mutase and where appropriate a 3-hydroxy-carbonyl-CoA ester-producing enzyme or enzyme system or a microorganism including them. The invention preferably relates to a biotechnological process for producing 2-hydroxy-2-methyl carboxylic acids, where microorganisms which have the desired activities are cultured in an aqueous system with the aid of simple natural products and convert intracellularly formed 3-hydroxy-carbonyl-CoA esters into the corresponding 2-hydroxy-2-methyl carboxylic acids. The invention likewise encompasses the production of unsaturated 2-methyl carboxylic acids, where the 2-hydroxy-2-methyl carboxylic acids obtained are converted by dehydration into the corresponding unsaturated 2-methyl carboxylic acids (methacrylic acid and higher homologues). | 02-11-2010 |
20100159545 | METHOD FOR PRODUCTION OF COROSOLIC ACID IN SUSPENSION CULTURE OF PLANT CELLS - The present invention relates to a method of producing corosolic acid by using plant cells that produce corosolic acid. More particularly, the present invention relates to a method of producing corosolic acid by using plant cell suspension cultures comprising the steps of: inducing a callus from a tissue of a plant producing corosolic acid; preparing a cell line capable of being cultured in liquid culture medium from the induced callus; culturing the cell line in a suspension culture; and isolating corosolic acid from the culture solution. The present invention has advantage of maximizing productivity by utilizing two-stage culture, treatment with inducing agent, and high cell-density culture in the suspension culture of plant cells producing corosolic acid. | 06-24-2010 |
20100173371 | Medium Comprising Fermented Korean Hot Pepper Paste Or Soybean Sauce And Production Method Of Gamma - Aminobutyric Acid - The present invention relates to a medium composition comprising fermented Korean hot pepper paste, undiluted solution of brewed soy sauce or acid hydrolyzed soy sauce, and a method for producing gamma-aminobutyric acid (GABA) using the medium. More precisely, the present invention relates to a medium composition for culturing | 07-08-2010 |
20100184172 | SYSTEM FOR THE PRODUCTION OF AROMATIC MOLECULES IN STREPTOMYCES - A process for the production of natural ferulic acid, coniferyl alcohol and/or vanillin, includes the bio-conversion of eugenol by a bacteria belonging to the | 07-22-2010 |
20100190224 | ENZYME FOR THE PRODUCTION OF METHYLMALONYL-COENZYME A OR ETHYLMALONYL-COENZYME A AND USE THEREOF - The present invention relates to an isolated DNA, which is selected from the following sequences:
| 07-29-2010 |
20100196976 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 08-05-2010 |
20100216199 | Production of long chain unbranched beta-poly(L-malic acid) by large scale Physarum cultivation and high-grade purification of the same - The invention is a large-scale production and purification of beta-poly(L-malic acid), biodegradable natural polyester of L-malic acid of molecular weight 30,000 to 300,000 from plasmodia of the Physarum family, especially Physarum polycephalum. This will replace previous laborious and uncontrolled production by bioreactor methods of high productivity and quality, especially to obtain pure polymer of high molecular weight. The described 20-liter bioreactor method can be scaled up for industrial purpose at optimal production and minimum growth time to prevent degradation The invention includes the method of isolation of polymalic acid of 100,000 Mn (number-averaged molecular weight) from the culture broth optimized towards high yields of extremely pure polymalic acid. The non-hygroscopic, water and organic solvent-soluble polymer is endotoxin and agglutinin free and ready to use in chemical syntheses. Because of its high molecular weight and purity it offers a wide spectrum of applications in pharmacy and medicine. | 08-26-2010 |
20100221797 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 09-02-2010 |
20100240109 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 09-23-2010 |
20100240110 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 09-23-2010 |
20100240111 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 09-23-2010 |
20100279368 | Biocatalystic Synthesis of Quinic Acid and Conversion to Hydroquinone by Recombinant Microbes - A bioengineered synthesis scheme for the production of quinic acid from a carbon source is provided. Methods of producing quinic acid from a carbon source based on the synthesis scheme as well as conversion of quinic acid to hydroquinone are also provided. | 11-04-2010 |
20100304453 | MICROORGANISMS AND METHODS FOR CONVERSION OF SYNGAS AND OTHER CARBON SOURCES TO USEFUL PRODUCTS - A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. | 12-02-2010 |
20100311133 | ELECTRODE, ENZYME SENSOR USING THE ELECTRODE, FUEL BATTERY, ELECTRONIC DEVICE, AND METHOD FOR DECOMPOSING POLYOL - An electrode that can generate reduced coenzymes from a desired substrate in multiple stages is provided. An electrode includes a 2-hydroxycarboxylic acid synthase, a 2-oxo acid synthase, and a 2-oxo acid carboxylyase. The electrode includes a 2-oxo acid carboxylyase, which is a decarboxylase, in addition to a 2-hydroxycarboxylic acid synthase and a 2-oxo acid synthase, which are oxidoreductases; therefore, oxidation products having carboxyl groups, reactions of which do not proceed with oxidoreductases, can be decarboxylated and modified into substances with which oxidoreductases can act. Thus, oxidation reactions and decarboxylation reactions can repeatedly proceed with respect to desired substrates, and large quantities of reduced coenzymes can be generated thereby. | 12-09-2010 |
20110020885 | USE OF GLYPHOSATE TO PRODUCE SHIKIMIC ACID IN MICROORGANISMS - The present invention provides methods for producing shikimic acid. In particular the invention provides methods for producing and isolating shikimic acid from a microorganism. Additionally, the invention provides methods for synthesizing compounds such as oseltamivir and 6-fluoroshikimic acid using shikimic acid produced from microorganisms. | 01-27-2011 |
20110020886 | METHOD FOR PRODUCING POLYHYDROXYALKANOIC ACID - Provided is a method for producing a polyhydroxyalkanoic acid (PHA). More particularly, a method of preparing PHA containing a high content of long-chain aromatic monomer-units by growing a phaZ mutant of a | 01-27-2011 |
20110033905 | PROCESS FOR PRODUCING OPTICALLY-ACTIVE ORTHO-SUBSTITUTED MANDELIC ACID COMPOUND - The present invention provides a process of asymmetrically reducing an ortho-substituted phenylglyoxalic acid compound to produce a corresponding optically-active ortho-substituted mandelic acid compound by using a microorganism having an ability of reducing the ortho-substituted phenylglyoxalic acid compound into the optically-active ortho-substituted mandelic acid compound, or a treated product thereof. | 02-10-2011 |
20110086398 | CELLULAR PRODUCTION OF HYDROXYVALERATES FROM LEVULINATE - The invention relates to cells that recombinantly express tesB and produce one or more hydroxyacids such as 3-hydroxyvalerate (3HV) and/or 4-hydroxyvalerate (4HV). | 04-14-2011 |
20110117617 | METHODS AND GENETICALLY ENGINEERED MICRO-ORGANISMS FOR THE COMBINED PRODUCTION OF PDO, BDO AND PHP BY FERMENTATION - The present invention relates to methods and genetically engineered micro-organisms for the combined production of PDO, BDO, and PHP by fermentation. The micro-organism is characterized in that its D-lactate dehydrogenase gene has been deleted or functionally inactivated, and it comprises heterogenous polynucleotides encoding the Coenzyme A-dependent Aldehyde dehydrogenase and the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms are also disclosed | 05-19-2011 |
20110136190 | RECOMBINANT BACTERIA FOR PRODUCING GLYCEROL AND GLYCEROL-DERIVED PRODUCTS FROM SUCROSE - Recombinant bacteria capable of producing glycerol and glycerol-derived products from sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct: a nucleotide sequence encoding a polypeptide having sucrose transporter activity; a nucleotide sequence encoding a polypeptide having fructokinase activity; and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. These recombinant bacteria are capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid. | 06-09-2011 |
20110151530 | ENZYMATIC PRODUCTION OF 2-HYDROXY-ISOBUTYRATE (2-HIBA) - The present invention concerns a new method for the biological preparation of 2-hydroxy-isobutyrate (2-HIBA), including a fermentation method with microorganisms modified to favour production of 2-HIBA from renewable resources. The invention also concerns the modified microorganisms used in such fermentation method. | 06-23-2011 |
20110159557 | COMPOSITIONS AND METHODS OF PGL FOR THE INCREASED PRODUCTION OF ISOPRENE - Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity. | 06-30-2011 |
20110236938 | BIOFUEL PRODUCTION - Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same. | 09-29-2011 |
20110236939 | BIOFUEL PRODUCTION - Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same. | 09-29-2011 |
20110244535 | Production Of Peracids Using An Enzyme Having Perhydrolysis Activity - A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided. | 10-06-2011 |
20110244536 | FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY EFFECTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES - Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade. | 10-06-2011 |
20110256598 | CO-METABOLISM OF FRUCTOSE AND GLUCOSE IN MICROBIAL PRODUCTION STRAINS - Recombinant microorganisms having an improved ability to co-metabolize in medium both fructose and glucose are described. The recombinant microorganisms comprise a promoter operably linked to a native or non-native nucleotide sequence that encodes a fucose:H+ symporter polypeptide. The recombinant microorganisms are useful for the production of a variety of products including glycerol and glycerol derivatives such as 1,3-propanediol and 3-hydroxypropionic acid. | 10-20-2011 |
20110269198 | METHODS FOR CONTROL OF FLUX IN METABOLIC PATHWAYS - The invention pertains to a method for preparing cells that can be used as biocatalysts by inducing in them a growth-decoupled state, in which interferase inhibits the expression of genes except the ones that code for the pathway enzymes of interest. mRNAs that code for interferase-resistant products are overexpressed in the background of a metabolically-frozen cell. Enzymes that compete for a substrate or product of the pathway of interest may be altered such that the enzyme is sensitive to a site-specific protease, which protease is inducible in the host cell. | 11-03-2011 |
20120028321 | High Solids Fermentation for Synthesis of Polyhydroxyalkanoates From Gas Substrates - Production of polyhydroxyalkanoates (PHAs) is performed by delivering substrates such as methane in gas phase during a high solids fermentation. Microorganisms are grown under balanced conditions, then gas phase substrates are delivered under unbalanced conditions to produce PHA granules inside the cells. The cells containing these granules are lysed and the bioplastic powder recovered. The balanced phase growth may occur in submerged liquid cultures or attached as biofilms to a surface. | 02-02-2012 |
20120045805 | SYSTEM AND METHOD FOR THE SYNTHESIS OF THERMOPLASTIC BIOPOLYMER - The various embodiments herein provide a system and a method of synthesis of thermoplastic biopolymer such as poly-β-hydroxy butyric acid wherein the activated sludge and synthetic waste water are acclimatized in a sequential batch reactor. The diazotrophs are reacted with CO | 02-23-2012 |
20120077238 | METHODS AND SYSTEMS FOR PRODUCTION OF POLYHYDROXYALKANOATE - Several embodiments of the invention relate generally to a system and methods for the treatment of gaseous emissions comprising methane and one or more non-methane compounds that can influence the metabolism of methane-oxidizing microorganisms. In several embodiments, there is provided a system and methods for the treatment of methane emissions through the use of methanotrophic microorganisms to generate functionally consistent and harvestable products. Certain embodiments of the invention are particularly advantageous because they reduce environmentally-destructive methane emissions and produce harvestable end-products. | 03-29-2012 |
20120094344 | METHOD FOR PRODUCING GLYCOLIC ACID - Provided is a production method of glycolic acid having a first step of preparing glycolonitrile from formaldehyde and hydrocyanic acid and a second step of hydrolyzing the glycolonitrile into glycolic acid directly or via a glycolate salt, which method can produce glycolic acid in easy production and purification steps while consuming less energy. In the production method, by carrying out the first and second steps continuously or by storing the glycolonitrile obtained in the first step at pH 4 or less and carrying out a hydrolysis reaction of the second step at from pH 5 to 9, a production yield of glycolic acid, activity for the production of glycolic acid and accumulated concentration of glycolic acid are improved, resulting in the production of glycolic acid having an improved purity and quality. | 04-19-2012 |
20120107886 | Lignin Oxidation and Products Thereof - Provided herein is a method of oxidizing lignin. Further disclosed herein are aromatic and non-aromatic compounds obtained from oxidized lignin. | 05-03-2012 |
20120122168 | RECOMBINANT ESCHERICHIA COLI HAVING ENHANCED ACETYL-COENZYME A SYNTHETASE ACTIVITY FOR PRODUCING GLYEROL AND GLYCEROL-DERIVED PRODUCTS - Recombinant | 05-17-2012 |
20120178136 | FERMENTATION PROCESS FOR PRODUCING GLYCOLIC ACID - The present invention relates to a process of fermentation for producing glycolic acid under specific pH conditions with an increase of the pH during fermentation. The invention relates more particularly to a method for producing glycolic acid by fermentation, which comprises culturing a microorganism having glycolic acid producing ability in an appropriate culture medium with a carbon source, and under specific pH conditions with an increase of the pH during fermentation, and recovery of glycolic acid from the culture medium. | 07-12-2012 |
20120220001 | METHOD FOR THE ENZYMATIC PRODUCTION OF 3-HYDROXY-S-METHYLBUTYRIC ACID FROM ACETONE AND ACETYL-COA - Described is a method for the production of 3-hydroxy-3-methylbutyric acid by enzyme-catalyzed covalent bond formation between the carbon atom of the oxo group of acetone and the methyl group of a compound which provides an activated acetyl group. Also described are recombinant organisms which produce 3-hydroxy-3-methylbutyric acid, and related compositions and methods. | 08-30-2012 |
20120270287 | NOVEL METHOD OF PRODUCING 3-HYDROXYPROPIONIC ACID FROM GLYCEROL - The present invention relates to a novel method of producing 3-hydroxypropionic acid from glycerol, and more particularly to a method of producing 3-hydroxypropionic acid by culturing in a glycerol-containing medium a mutant microorganism obtained by amplifying an aldehyde dehydrogenase-encoding gene in a microorganism having the abilities to produce coenzyme B12 and produce 3-hydroxypropionic acid using glycerol as a carbon source. The present invention enables the fermentation of glycerol even under microaerobic or aerobic conditions without having to add coenzyme B12. Thus, the invention will be very suitable for the development of biological processes for producing large amounts of 3-hydroxypropionic acid. | 10-25-2012 |
20120276603 | PRODUCTION OF MEVALONATE, ISOPRENE, AND ISOPRENOIDS USING GENES ENCODING POLYPEPTIDES HAVING THIOLASE, HMG-COA SYNTHASE AND HMG-COA REDUCTASE ENZYMATIC ACTIVITIES - The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organisms | 11-01-2012 |
20120276604 | MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID - The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. | 11-01-2012 |
20120276605 | MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID - The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. | 11-01-2012 |
20120301935 | RECOMBINANT MICROORGANISM FOR SIMULTANEOUSLY PRODUCING 3-HYDROXYPROPIONIC ACID AND 1,3 PROPANEDIOL - A method of simultaneously producing 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) using a microorganism is provided. The method includes converting glycerol into 3-HP and 1,3-PDO using a recombinant microorganism having both 3-HP and 1,3-PDO producing genes. | 11-29-2012 |
20120315682 | METHOD FOR PRODUCING HIGH AMOUNT OF GLYCOLIC ACID BY FERMENTATION - The present invention relates to an improved method for the bioconversion of a fermentable carbon source to glycolic acid by a recombinant microorganism bearing new genetic modifications such as ΔldhA, ΔmgsA, ΔarcA, and ΔlldP, ΔglcA, ΔyjcG and combination of them allowing a production with higher yield, titer and productivity. | 12-13-2012 |
20120329110 | GENETIC MODIFICATION FOR PRODUCTION OF 3-HYDROXYPROPIONIC ACID - A method of increasing 3-HP production efficiency by inhibiting expression of a lactate dehydrogenase, a phosphotransacetylase, and an alcohol dehydrogenase in production of 3-HP using a malonic semialdehyde reduction pathway to prevent metabolite leak and increase a malonyl-CoA pool is disclosed. | 12-27-2012 |
20130017584 | Enzymatic Hydroxylation of Aliphatic HydrocarbonAANM Hofrichter; MartinAACI DresdenAACO DEAAGP Hofrichter; Martin Dresden DEAANM Scheibner; KatrinAACI JenaAACO DEAAGP Scheibner; Katrin Jena DEAANM Ullrich; ReneAACI ZittauAACO DEAAGP Ullrich; Rene Zittau DEAANM Kinne; MatthiasAACI GorlitzAACO DEAAGP Kinne; Matthias Gorlitz DEAANM Peter; SebastianAACI ZittauAACO DEAAGP Peter; Sebastian Zittau DEAANM Lund; HenrikAACI VaerloeseAACO DKAAGP Lund; Henrik Vaerloese DKAANM Kalum; LisbethAACI VaerloeseAACO DKAAGP Kalum; Lisbeth Vaerloese DK - The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons. | 01-17-2013 |
20130029391 | METHOD OF PREPARING KODA USING LEMNA PAUCICOSTATA - After identifying the genes of a novel lipoxygenase and a novel allene oxide synthase derived from Lemna paucicostata SH strain, a plant growth regulating agent (KODA) was produced at high yield by using a Lemna paucicostata strain that expresses the lipoxygenase and the allene oxide synthase. | 01-31-2013 |
20130029392 | METHOD FOR PRODUCING HYDROXYCARBOXYLIC ACID BY ENHANCING SYNTHESIS OF COENZYME - Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase. | 01-31-2013 |
20130045518 | VARIANT SUCROSE TRANSPORTER POLYPEPTIDES THAT ENABLE FASTER SUCROSE UTILIZATION IN BACTERIA - Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria are described. Additionally, variant or recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided. | 02-21-2013 |
20130045519 | RECOMBINANT BACTERIA HAVING IMPROVED SUCROSE UTILIZATION - Recombinant bacteria having an improved ability to utilize sucrose are provided. These recombinant bacteria have nucleotide sequences encoding sucrose utilization polypeptides integrated into their genome between the yihP gene or its homolog and the yihO gene or its homolog. Additionally, methods of utilizing the recombinant bacteria to produce products such as glycerol and glycerol-derived products are provided. | 02-21-2013 |
20130065287 | CHEMICAL PROCESSING CELL WITH NANOSTRUCTURED MEMBRANES - A chemical processing cell includes an upstream membrane and a downstream membrane. The upstream membrane generates a first reaction product. The downstream membrane converts the first reaction product to a second reaction product. | 03-14-2013 |
20130071898 | HOST CELLS AND METHODS FOR PRODUCTION OF ISOBUTANOL - Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity. | 03-21-2013 |
20130089903 | USE OF INDUCIBLE PROMOTERS IN THE PRODUCTION OF GLYCOLIC ACID - The present invention relates to use of inducible promoters in the production of glycolic acid by fermentation. The present invention concerns a method for the production of glycolic acid in a fermentative process comprising the following steps:
| 04-11-2013 |
20130095541 | METHOD FOR PREPARING 3-HYDROXYPROPIONIC ACID FROM GLYCEROL IN HIGH YIELD - The present invention relates to a method for producing 3-hydroxypropionic acid by culturing in a glycerol-containing medium a mutant microorganism obtained by inserting or amplifying a gene encoding propanediol utilization protein in a microorganism having the abilities to produce coenzyme B12 and produce 3-hydroxypropionic acid using glycerol as a carbon source | 04-18-2013 |
20130130340 | BIOSYNTHESIS OF CAFFEIC ACID AND CAFFEIC ACID DERIVATIVES BY RECOMBINANT MICROORGANISMS - Microorganisms are genetically engineered to synthesize caffeic acid from simple carbon sources via a tyrosine intermediate by means of a dual pathway that utilizes both endogenous and engineered enzymatic activities. | 05-23-2013 |
20130196397 | COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS - The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO. | 08-01-2013 |
20130210097 | GLYCOLIC ACID FERMENTATIVE PRODUCTION WITH A MODIFIED MICROORGANISM - The present invention is related to a method for the fermentative production of glycolic acid, its derivatives or precursors, comprising the culture of an | 08-15-2013 |
20130230892 | VARIANT SUCROSE TRANSPORTER POLYPEPTIDES - Variant sucrose transporter polypeptides that enable bacterial growth over a wide range of gene expression levels and sucrose concentrations are described. Additionally, recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided | 09-05-2013 |
20130230893 | RECOMBINANT BACTERIA COMPRISING NOVEL SUCROSE TRANSPORTERS - Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described. | 09-05-2013 |
20130273623 | NOVEL METHOD OF PRODUCTION OF 2,4-DIHYDROXYBUTIC ACID - The present invention deals with a method of producing 2,4-dihydroxybutyric acid (2,4-DHB) by a synthetic pathway comprising the transformation of malate in 4-phospho-malate using a malate kinase, said 4-phospho-malate being transformed in malate-4-semialdehyde using a malate semialdehyde dehydrogenase and said malate-4-semialdehyde being transformed in 2,4-DHB using a DHB dehydrogenase. | 10-17-2013 |
20130280774 | DIRECT STARCH TO FERMENTABLE SUGAR AS FEEDSTOCK FOR THE PRODUCTION OF ISOPRENE, ISOPRENOID PRECURSOR MOLECULES, AND/OR ISOPRENOIDS - Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism, such as isoprene, isoprenoid precursor molecules, and/or isoprenoids. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains. | 10-24-2013 |
20130288323 | MICROBIAL PRODUCTION OF POLYHYDROXYALKANOATES - A process for producing medium-chain-length 3-hydroxyalkanoic acids in a liquid medium, the process comprising: (i) intermixing a liquid culture medium and a microbial inoculum comprising a pure culture of a | 10-31-2013 |
20130302867 | METHOD FOR PRODUCING BIODEGRADABLE POLYMER AND BIOMASS FUEL CONVERTED FROM CARBON SOURCE BY RECOMBINANT MICROORGANISMS - Disclosed is a method for producing biodegradable polymer and biomass fuel converted from carbon source by using recombinant microorganisms, comprising the steps of: (A) providing recombinant microorganisms transformed with plasmids containing at least a gene encoding for glycerol utilizing enzyme and a gene encoding for polyhydroxyalkanoate synthase; (B) culturing the recombinant microorganisms in a medium containing glycerol; (C) inducing expression of the genes of step (A), thereby obtaining polyhydroxyalkanoate and ethanol; and (D) recovering the polyhydroxyalkanoate and the ethanol; wherein the recombinant microorganisms have a glycerol utilization rate more than 90% (w/w), and have polyhydroxyalkanoate accumulated therein to a biomass content thereof at least 30% (w/w). | 11-14-2013 |
20130316413 | REVERSE BETA OXIDATION PATHWAY - The invention relates to recombinant microorganisms that have been engineered to produce various chemicals using genes that have been repurposed to create a reverse beta oxidation pathway. Generally speaking, the beta oxidation cycle is expressed and driven in reverse by modifying various regulation points for as many cycles as needed, and then the CoA thioester intermediates are converted to useful products by the action of termination enzymes. | 11-28-2013 |
20130323808 | 3-HYDROXYPROPIONIC ACID AND OTHER ORGANIC COMPOUNDS - Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed. | 12-05-2013 |
20130344551 | KETOL-ACID REDUCTOISOMERASE USING NADH - Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from | 12-26-2013 |
20140011249 | METHODS FOR INCREASING PRODUCT YIELDS - A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product. | 01-09-2014 |
20140030779 | MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS - The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms. | 01-30-2014 |
20140045231 | METHODS FOR PRODUCING 3-HYDROXYPROPIONIC ACID AND OTHER PRODUCTS - This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid. | 02-13-2014 |
20140051140 | PROCESS OF BIOLOGICALLY PRODUCING A p-HYDROXYBENZOIC ACID - A method of biologically producing p-hydroxybenzoic acid, and a method for producing p-hydroxybenzoic acid from lignin through chemical and biological conversion. | 02-20-2014 |
20140065681 | Compositions and methods for 3-hydroxypropionic acid production - The present application discloses genetically modified yeast cells comprising an active 3-HP fermentation pathway, and the use of these cells to produce 3-HP. | 03-06-2014 |
20140073024 | Phenylpyruvate Reductase and Method for Manufacturing Optically-Active Phenyllactic Acid and 4-Hydroxyl-Phenyllactic Acid Using Same Enzyme - The present invention provides a phenylpyruvate reductase for efficiently obtaining highly pure, optically active 3-phenyllactate and 4-hydroxyphenyllactate; a gene coding for the same; and a method for manufacturing optically active 3-phenyllactate and 4-hydroxyphenyllactate employing the same. | 03-13-2014 |
20140093927 | Variant Sucrose Transporter Polypeptides That Enable Faster Sucrose Utilization In Bacteria - Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria are described. Additionally, variant or recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided. | 04-03-2014 |
20140147899 | VARIANT SUCROSE TRANSPORTER POLYPEPTIDES - Variant sucrose transporter polypeptides that enable bacterial growth over a wide range of gene expression levels and sucrose concentrations are described. Additionally, recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided | 05-29-2014 |
20140154760 | 3-Hydroxypropionic Acid Production By Recombinant Yeasts - Provided herein are recombinant yeast cells having an active 3-Hydroxypropionic Acid (3-HP) pathway and further comprising a heterologous polynucleotide encoding a non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN). Also described are methods of using the recombinant yeast cells to produce 3-HP and acrylic acid. | 06-05-2014 |
20140154761 | PROCESS OF BIOLOGICALLY PRODUCING AROMATIC CARBOXYLIC ACID AND DERIVATIVE THEREOF - A method of biologically producing an aromatic carboxylic acid by contacting a substrate containing an aromatic carboxylic acid having a para-hydroxy group with a biocatalyst that removes the para-hydroxy group. | 06-05-2014 |
20140212938 | Dehydrogenase Variants and Polynucleotides Encoding Same - The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 07-31-2014 |
20140234926 | RECOMBINANT ANAEROBIC ACETOGENIC BACTERIA FOR PRODUCTION OF ISOPRENE AND/OR INDUSTRIAL BIO-PRODUCTS USING SYNTHESIS GAS - This invention provides for recombinant anaerobic acetogenic bacterial cells having one or more nucleic acids whereby isoprene, mevalonate and/or other industrial bio-products are produced in a substantially oxygen-free culture condition using synthesis gas as energy and/or carbon source. | 08-21-2014 |
20140234927 | PRODUCTION METHOD FOR ORGANIC ACID USING COA-TRANSFERASE - The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of the organic acid-CoA using CoA transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-CoA using acetyl-CoA as a substrate and a CoA transferase gene. | 08-21-2014 |
20140295510 | Eukaryotic cell and method for producing glycolic acid - The present invention concerns a eukaryotic host selected from microorganisms, and a method for producing glycolic acid using said eukaryotic host cells, especially cells of a genetically modified fungal host. Further this invention relates to a glycolic acid product obtained using the method described here and the use of genetically modified microorganism cells in production of glycolic acid. | 10-02-2014 |
20140302574 | 3-HYDROXYPROPIONIC ACID AND OTHER ORGANIC COMPOUNDS - Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed. | 10-09-2014 |
20140363863 | PROCESS FOR PRODUCING 3-HYDROXYBUTYRIC ACID OR SALT THEREOF - The invention provides a process for producing 3-hydroxybutyric acid or a salt thereof. The process includes (1) culturing one or more halophilic bacteria belonging to the genus | 12-11-2014 |
20140377823 | BETA-ALANINE/ALPHA-KETOGLUTARATE AMINOTRANSFERASE FOR 3-HYDROXYPROPIONIC ACID PRODUCTION - The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof. | 12-25-2014 |
20150044746 | METHOD OF ENHANCED BIOPRODUCTION - Bio-based renewable 3-hydroxypropionic acid (3-HP) may be produced through fermentation processes utilizing genetically modified microorganisms such as, for example, genetically modified | 02-12-2015 |
20150064754 | BIOPRODUCTION OF CHEMICALS - The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products. | 03-05-2015 |
20150064755 | RECOMBINANT BACTERIA COMPRISING NOVEL SUCROSE TRANSPORTERS - Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described. | 03-05-2015 |
20150087036 | Host Cells and Method for Making Acrylate and Precursors Thereof Using an Odd-Numbered Alkane Feedstock - Provided herein is an alkane-metabolizing cell that is unable to convert propionyl-CoA into methylmalonyl-CoA or 2-metylcitrate synthase. Depending on which enzymes are present in the cell, the cell can produce acrylate or a precursor for the same (e.g., propionate, 3-hydroxypropionyl-CoA, 3-hydroxypropionate, acrylyl-CoA) that can be readily converted to acrylate enzymatically (e.g., in the cell) or by chemical treatment. In one embodiment, the cell may contain a cytochrome P450 or alkane oxidase enzyme that allows the production of 3-hydroxypropionyl-CoA, which can be readily converted to 3-hydroxypropionate. In order to make such compounds, the cell may be grown in the presence of an odd-numbered chain alkane (e.g., pentane or heptane), although another odd-numbered chain alkane may be used. In another embodiment, the cell may contain acyl-CoA oxidase, enoyl-CoA hydratase, and hydrolase. | 03-26-2015 |
20150093795 | Cutinase Variants and Polynucleotides Encoding Same - The present invention relates tocutinasevariants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 04-02-2015 |
20150125918 | ACID-RESISTANCE IN KLUYVEROMYCES MARXIANUS BY ENGINEERING TRANSCRIPTIONAL FACTOR - A microorganism with improved acid-resistance. A microorganism capable of efficiently producing 3-HP, and methods for producing an organic acid. | 05-07-2015 |
20150125919 | MICROORGANISM WITH INCREASED IRON-REGULATED ABC TRANSPORTER ACTIVITY AND METHOD OF PRODUCING HYDROXYCARBOXYLIC ACID BY USING THE MICROORGANISM - A recombinant microorganism having increased iron-regulated ABC transporter activity and increased hydroxycarboxylic acid production, as well as a method of producing a hydroxycarboxylic acid using the recombinant microorganism, and a method of producing the recombinant microorganism. | 05-07-2015 |
20150147793 | METHOD FOR THE PREPARATION OF 2,4-DIHYDROXYBUTYRATE - A method for the preparation of 2,4-dihydroxybutyric acid from homoserine includes a first step of conversion of the primary amino group of homoserine to a carbonyl group to obtain 2-oxo-4-hydroxybutyrate, and a second step of reduction of the obtained 2-oxo-4-hydroxybutyrate (OHB) to 2,4-dihydroxybutyrate. | 05-28-2015 |
20150299742 | COMPOSITIONS AND METHODS FOR ENHANCING TOLERANCE FOR THE PRODUCTION OF ORGANIC CHEMICALS PRODUCED BY MICROORGANISMS - Embodiments herein generally relate to methods, compositions and uses for enhancing tolerance of production of organic acids and alcohols by microorganisms. This application also relates generally to methods, compositions and uses of vectors having one or more genetic element to increase the tolerance of organic acids or alcohols by a microorganism. Certain embodiments relate to compositions and methods of enhancing the tolerance for production of 3-hydroxypropionic acid (3-HP) by bacteria. In some embodiments, compositions and methods relate to regulating the expression of an inhibitory molecule of an enhancing gene to increase production of organic acid by bacteria. | 10-22-2015 |
20150307855 | Producing 3-Hydroxycarboxylic Acid and Ketone Using Polyketide Synthases - The present invention provides for a polyketide synthase (PKS) capable of synthesizing a 3-hydroxycarboxylic acid or ketone. The present invention also provides for a host cell comprising the PKS and when cultured produces the 3-hydroxycarboxylic acid or ketone. | 10-29-2015 |
20150361456 | Enzymatic Hydroxylation of Aliphatic Hydrocarbon - The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons. | 12-17-2015 |
20150376580 | MUTANT ENZYMES - This invention relates to mutant enzymes with enhanced properties and processes for oxidation of organic compound substrates using such enzymes. | 12-31-2015 |
20160023930 | POLYHYDROXYALKANOATE PRODUCTION DURING WASTEWATER TREATMENT - A wastewater treatment process elicits microorganisms to convert a waste stream/organic resource to intracellular biopolymer polyhydroxyalkanoate (PHA). The process includes (i) waste stream/organic resource composition feed criteria, (ii) configuration coupled with operational parameters, and (iii) PHA-laden biomass separation and stabilization. A waste stream/organic resource capable of producing enhanced levels of PHA may be selected based on a combination of criteria, which may include short chain fatty acid concentration, protein concentration, polysaccharides concentration, and total suspended solids concentration. The waste stream is introduced into an aeration basin upon a specific configuration and operated under various parameter combinations for selecting/enriching microorganisms capable of producing PHA. The PHA-laden biomass is separated and stabilized for downstream PHA related product beneficial uses. The present process achieves concurrent wastewater treatment and PHA production, where PHA level (of more than 10% on a cell-weight basis) otherwise could not be obtained. | 01-28-2016 |
20160097063 | Production Of Enantiopure alpha-Hydroxy Carboxylic Acids From Alkenes By Cascade Biocatalysis - The invention provides compositions comprising an alkene epoxidase and a selective epoxide hydrolase, such as a recombinant microorganism comprising a first heterologous nucleic acid encoding an alkene epoxidase and a second heterologous nucleic acid encoding a selective epoxide hydrolase. Exemplary alkene epoxidases include StyAB, while exemplary selective epoxide hydrolases include epoxide hydrolases from | 04-07-2016 |
20160130613 | 3HP TOLERANCE - Cells and cell cultures are provided that have improved tolerance to 3-hydroxypropionic acid (3HP). Genetic modifications to provide a mutated or overexpressed SFA1 gene or other enhancement of 3HP detoxification via a glutathione-dependent dehydrogenase reaction, including medium supplementation with glutathione, may be combined with a 3HP producing metabolic pathway. | 05-12-2016 |
20160130614 | METHOD OF ENHANCED BIOPRODUCTION - Bio-based renewable 3-hydroxypropionic acid (3-HP) may be produced through fermentation processes utilizing genetically modified microorganisms such as, for example, genetically modified | 05-12-2016 |
20160138055 | BIOPRODUCTION OF CHEMICALS - The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products. | 05-19-2016 |
20160138056 | MICROBIAL PRODUCTION OF 3-HYDROXYPROPIONIC ACID - A yeast cell having a reduced level of activity of NAD dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has at least one exogenous gene encoding NADP dependent GAPDH and/or has up-regulation of at least one endogenous gene expressing NADP dependent GAPDH, wherein combined expression of the enzymes NADP dependent GAPDH, PDC, ALD, ACS, ACC* and MCR in said host cell increases metabolic flux towards 3-HP via malonyl-CoA compared to an otherwise similar yeast cell lacking said genetic modification. | 05-19-2016 |
20160145227 | PROCESSES FOR CONVERSION OF BIOLOGICALLY DERIVED MEVALONIC ACID - The invention relates to a process comprising reacting mevalonic acid, or a solution comprising mevalonic acid, to yield a first product or first product mixture, optionally in the presence of a solid catalyst and/or at elevated temperature and/or pressure. The invention further relates to a process comprising: (a) providing a microbial organism that expresses a biosynthetic mevalonic acid pathway; (b) growing the microbial organism in fermentation medium comprising suitable carbon substrates, whereby biobased mevalonic acid is produced; and (c) reacting said biobased mevalonic acid to yield a first product or first product mixture. | 05-26-2016 |
20160145652 | METHODS FOR INCREASING PRODUCT YIELDS - A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product. | 05-26-2016 |
20160168070 | CONVERSION OF CARBOHYDRATES TO LEVULINIC ACID ESTERS | 06-16-2016 |
20160168600 | 3-HYDROXYPROPIONIC ACID PRODUCTION BY RECOMBINANT YEASTS EXPRESSING AN INSECT ASPARTATE 1-DECARBOXYLASE | 06-16-2016 |
20160176799 | ISOLATION AND PURIFICATION OF SHIKIMIC ACID | 06-23-2016 |
20160186217 | METHOD FOR BIOCATALYTIC SYNTHESIS OF SUBSTITUTED OR UNSUBSTITUTED PHENYLACETIC ACIDS AND KETONES HAVING ENZYMES OF MICROBIAL STYRENE DEGRADATION - The present invention relates to a method for the biocatalytic synthesis of substituted and unsubstituted phenylacetic acids and ketones from styrenes and bicyclic aromatic hydrocarbons using enzymes of microbial styrene degradation in a whole-cell sensor, as well as a kit for the biocatalytic synthesis of substituted and unsubstituted phenylacetic acids and ketones containing a whole-cell catalyst and the use of the method, wherein the method comprises the following steps:
| 06-30-2016 |
20160201094 | METHODS FOR BIOSYNTHESIZING METHACRYLATE | 07-14-2016 |