| Entries |
| Document | Title | Date |
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| 20110094884 | SURFACE CHARGE ENABLED NANOPOROUS SEMI-PERMEABLE MEMBRANE FOR DESALINATION - A filter includes a membrane having a plurality of nanochannels formed therein. A first surface charge material is deposited on an end portion of the nanochannels. The first surface charge material includes a surface charge to electrostatically influence ions in an electrolytic solution such that the nanochannels reflect ions back into the electrolytic solution while passing a fluid of the electrolytic solution. Methods for making and using the filter are also provided. | 04-28-2011 |
| 20130319860 | METHOD AND APPARATUS FOR SENSING ION CONCENTRATIONS IN A FLUID SAMPLE - The invention provides a method for the measurement of a concentration of a charged species in a sample, the sample having a plurality of types of charged species and at least one insoluble component. The method comprises: providing the sample on a surface of a partly permeable layer; allowing components of the sample to pass through the partly permeable layer into a channel; and separating the components into sections, such that each at least one of the sections substantially comprises a single type of the plurality of the types of charged species, and determining the charge concentration in the at least one of the sections. | 12-05-2013 |
| 20160130639 | System and Method for Detection of Nucleic Acids - Embodiments provide detection systems and methods for detecting the presence of a nucleic acid in one or more samples. In a detection method, a sample and one or more nucleic acid probes are introduced into a channel. A first potential difference is applied across the length of the channel in a first direction, and a first electrical property value is detected. Subsequently, a second potential difference is applied across the length of the channel in a second opposite direction, and a second electrical property value is detected. Presence or absence of a nucleic acid in the channel is determined based on a comparison between the first and second electrical property values. | 05-12-2016 |
| 20160024569 | NANOFLUIDIC DEVICES FOR THE RAPID MAPPING OF WHOLE GENOMES AND RELATED SYSTEMS AND METHODS OF ANALYSIS - Devices and methods generate an ordered restriction map of genomic DNA extracted from whole cells, nuclei, whole chromosomes, or other sources of long DNA molecules. The devices have a fluidic microchannel that merges into a reaction nanochannel that merges into a detection nanochannel at an interface where the nanochannel diameter decreases in size by between 50% to 99%. Intact molecules of DNA are transported to the reaction nanochannel and then fragmented in the reaction nanochannel using restriction endonuclease enzymes. The reaction nanochannel is sized and configured so that the fragments stay in an original order until they are injected into the detection nanochannel. Signal at one or more locations along the detection nanochannel is detected to map fragments in the order they occur along a long DNA molecule. | 01-28-2016 |
| 20120160680 | MICROFLUIDIC SYSTEM AND BUBBLE MANIPULATION METHOD THEREOF - A microfluidic system includes a first electrode plate having a first substrate and a first electrode layer, wherein the first electrode layer has a plurality of continuously-arranged driving electrodes; a second electrode plate having a second substrate and a second electrode layer, wherein the second electrode layer corresponds to the first electrode layer; a spacing structure disposed between the first electrode plate and the second electrode plate so as to define a fluidic space therebetween; at least one fluid manipulatably received in the fluidic space, wherein the fluid has at least one gas bubble having a reaction gas thereinside, and the gas bubble is an enclosure structure. An electric potential is applied for driving the fluid and then controlling the position of the gas bubble. A gas breakdown voltage is applied to electrically discharge the gas in the gas bubble. | 06-28-2012 |
| 20130087458 | MICRO-ASSAY CHIP, ASSAY DEVICE USING SAID MICRO-ASSAY CHIP AND PUMPING METHOD - A microanalysis chip includes: a main flow channel ( | 04-11-2013 |
| 20100006435 | Microfluidic System - A method for forming a microfluidic channel with improved flow characteristics for one or more analytes is disclosed. A microfluidic channel having modified surfaces is formed in fused silica. The fused silica surfaces are modified by the addition of a layer of borophosphosilicate glass. The addition of the borophosphosilicate glass results in an improved flow velocity profile of the analyte. As a result, control over the position and movement of analytes within the solution is improved. | 01-14-2010 |
| 20100006436 | HEMOGLOBIN MEASUREMENT METHOD AND ELECTROPHORESIS APPARATUS - An object of the present invention is to provide a method for measuring hemoglobin that enables short-time, high-accuracy measurement of hemoglobin, in particular stable hemoglobin A1c, which is used as a diagnostic indicator of diabetes mellitus, and an electrophoresis apparatus that is suitably used in this measurement method. The present invention provides a method for measuring hemoglobins using electrophoresis, which includes: using a migration path having an inner surface coated with a cationic substance to be immobilized on the inner surface or a migration path having an inner surface made of a cationic material; and using a buffer solution containing a water-soluble polymer having an anionic group as an electrophoresis buffer solution. | 01-14-2010 |
| 20110000788 | ANALYSIS AND ASSAY OF GLYCATED HAEMOGLOBINS BY CAPILLARY ELECTROPHORESIS, BUFFER COMPOSITIONS AND KITS FOR CAPILLARY ELECTROPHORESIS - The invention relates to a method for analysis by capillary electrophoresis of glycated haemoglobins comprising at least one globin chain comprising a glucose residue bound to the amino acid in the N-terminal position, contained in a biological sample, said method comprising using a buffer composition comprising at least one compound which is capable of specifically complexing glucose residues of one or several glycated haemoglobin(s) and of providing said glycated haemoglobin(s) with several negative electric charges at an alkaline pH. By way of example, this compound may be 3,4- or 3,5-dicarboxyphenylboronic acid, preferably 3,5-dicarboxyphenylboronic acid. | 01-06-2011 |
| 20160084805 | SYSTEM AND METHOD OF PRECONCENTRATING ANALYTES IN A MICROFLUIDIC DEVICE - A method and system for preconcentrating analytes at a microvalve in a microfluidic device is disclosed. The system includes a sample channel loaded with a sample solution. The sample channel includes a semi-permeable membrane microvalve. An electric potential is applied at or across the microvalve to preconcentrate the sample solution when the microvalve is closed. The method includes pretreatments of the device or valve for preconcentration of the analytes. For preconcentration of anionic analytes, the device is baked. For preconcentration of the cationic analytes, the surface of the membrane microvalve is coated with a polycationic coating, and the device is baked. | 03-24-2016 |
| 20130313113 | Microfluidic Processing of Target Species in Ferrofluids - Disclosed are systems, devices, methods, and other implementations, including a device to detect at least one target species in a sample, with the device including a microfluidic channel configured to receive the sample containing the at least one target species and a biocompatible ferrofluid in which the at least one target species is suspended, a detector to determine the at least one target species in the sample, and at least two of electrodes positioned proximate the microfluidic channel, the at least two electrodes configured to generate controllable magnetic forces in the sample containing the ferrofluid when a controllable at least one electrical current is applied to the at least two electrodes. The generated controllable magnetic forces causes the at least one target species to be directed towards the detector. Also disclosed are devices for separating target species in a ferrofluid, and for focusing target species suspended in a ferrofluid. | 11-28-2013 |
| 20120222958 | Nanopore Device for Reversible Ion and Molecule Sensing or Migration - Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore. | 09-06-2012 |
| 20160025702 | SYSTEMS, DEVICES AND METHODS FOR TRANSLOCATION CONTROL - Some embodiments of the present disclosure are directed to systems, methods and devices for controlling the transit of a molecule across a nanopore. Some embodiments are directed to a device comprising a first compartment, a second compartment, a first pair of electrodes comprising a first electrode provided in the first compartment and a second electrode provided in the second compartment, a partition separating the first compartment from the second compartment, an orifice provided in the partition, a second pair of electrodes arranged proximate the orifice, the second pair of electrodes being functionalized with molecules, and a tunnel gap comprising the spacing between the second pair of electrodes. | 01-28-2016 |
| 20160024567 | ELECTRONIC LABEL FREE DETECTION OF DNA COMPLEXES USING NANOGAP - A technique for electrical detection of a molecule is provided. A fluidic bridge is formed between a nanopipette and a fluid cell, where the molecule is in the nanopipette. A voltage difference is applied between the nanopipette and the fluid cell, where the fluid cell contains an electrolyte solution. Entry of the molecule into the fluidic bridge is determined by detecting a fore pulse. The fluidic bridge between the nanopipette and the fluid cell is broken to form a nanogap. In response to waiting a time interval, the fluidic bridge is reformed between the nanopipette and the fluid cell to close the nanogap. The molecule is determined to exit the nanopipette by detecting an after pulse. | 01-28-2016 |
| 20100252433 | NOVEL OPTICAL LABELING MOLECULES FOR PROTEOMICS AND OTHER BIOLOGICAL ANALYSES - The invention relates to compositions and methods useful in the labeling and identification of changes in protein levels, changes in enzyme activity, and changes in protein modification. The invention provides for highly soluble optical labeling molecules which are optionally cleavable after separation of mixtures of labeled proteins into components. These optical labeling molecules find utility in a variety of applications, including use in the field of proteomics. | 10-07-2010 |
| 20110220500 | Glucuronidated Acetaminophen as a Marker of Hepatic Disorders - The present invention provides methods for diagnosing human hepatic disorders, such as non-alcoholic steatohepatitis (NASH), identifying a human subject at risk of an adverse drug reaction, and determining an appropriate dosage of a therapeutic drug for a human subject. The marker is an increased level of acetaminophen-glucuronide in a plasma or urine sample from a human subject. | 09-15-2011 |
| 20100051461 | Poly and Copolyn(N-Vinylamide)s and their use in Capillary Electrophoresis - The invention relates generally to polymers and copolymers comprising N-vinylamide-type monomers, their preparation, and compositions, such as electrophoresis separation media, containing the same; to supports, such as capillaries, containing these polymers; and methods for separating a mixture of biomolecules, especially polynucleotides, using capillary electrophoresis. Separation media comprising such polymers yield advantageous performance in the analysis and separation of biomolecules by capillary electrophoresis. | 03-04-2010 |
| 20100051460 | MICROFLUIDIC SAMPLE DETECTION - Disclosed is a method for sample detection by providing one or more samples to a microfluidic device including one or more microfluidic channels; and controlling one or more droplets in the channels to increase a likelihood of association between the one or more samples and one or more probes. | 03-04-2010 |
| 20120118737 | Method And Markers For Diagnosing Acute Renal Failure - The invention relates to a method for diagnosing acute renal failure, comprising the step of determining a presence or absence or amplitude of at least three polypeptide markers in a sample, wherein the polypeptide marker is among the markers characterized in table 1 by values for the molecular weights and the migration time. | 05-17-2012 |
| 20090294287 | MICROCHIP ELECTROPHORESIS METHOD AND DEVICE - An separation method comprising a temperature control process useful for microchip electrophoresis such as microchip DGGE is provided along with a device therefor. | 12-03-2009 |
| 20160081786 | IMPLANTABLE SYSTEMS AND METHODS FOR REMOVING SPECIFIC IMPURITIES FROM FLUIDS SUCH AS BLOOD - Systems and methods utilize semipermeable nanotubes in conjunction with application of controlled electrical potentials across semipermeable nanotube walls allow selective transport of charged impurities (e.g., charged impurities, ions, etc.) from a fluid into these nanotubes. Impurities collected in these nanotubes can then be removed from the fluid, (e.g., blood) as a waste stream. A collection of semipermeable nanotubes each carrying a waste stream can be aggregated and merged into a ureter for excretion thereby providing an artificial kidney system. Sensors that detect/measure various impurities may be included in the system to feed information to a microprocessor to inform on concentrations of impurities, and thereby control electrical potentials applied to the system. | 03-24-2016 |
| 20110005931 | APPARATUS AND METHOD FOR MANIPULATING MICRO COMPONENT - In one embodiment, a system for manipulation of a micro component includes a gripper subsystem for lifting, holding, and releasing a micro component. The gripper subsystem includes a base substrate having a work side and an opposing side, a positive electrode secured to the work side of the base substrate, a negative electrode suitably spaced from the positive electrode and secured to the work side of the base substrate, a dielectric layer formed over the work side of the base substrate and the positive and negative electrodes, and a hydrophobic layer comprising a hydrophobic material with predictable electrowetting behavior formed over the dielectric layer such that the dielectric layer is between the work side of the base substrate and the hydrophobic layer. A method for manipulation of a micro component is also provided as well as a method of manufacturing the system for manipulation of a micro component. | 01-13-2011 |
| 20110114486 | CONTINUOUS BIOMOLECULE SEPARATION IN A NANOFILTER - This invention provides a method and an apparatus for quickly continuously fractionating biomolecules, such as DNAs, proteins and carbohydrates by taking advantage of differential bidirectional transport of biomolecules with varying physico-chemical characteristics, for example size, charge, hydrophobicity, or combinations thereof, through periodic arrays of microfabricated nanofilters. The passage of biomolecules through the nanofilter is a function of both steric and electrostatic interactions between charged macromolecules and charged nanofilter walls, Continuous-flow separation through the devices of this invention are applicable for molecules varying in terms of any molecular properties (e.g., size, charge density or hydrophobicity) that can lead to differential transport across the nanofilters. | 05-19-2011 |
| 20110083962 | ADSORPTION PREVENTION METHOD OF INNER-WALL-COATED CAPILLARY FOR CAPILLARY ELECTROPHORESIS, INNER-WALL-COATED CAPILLARY FOR CAPILLARY ELECTROPHORESIS, MANUFACTURING METHOD THEREOF, AND METHOD FOR SIMULTANEOUS ANALYSIS OF PHOSPHORYLATED COMPOUND AND ANION BY CAPILLARY ELECTROPHORESIS - A inner-wall-coated capillary in which a zwitterionic polymer including a phosphate group including, for example, a phosphorylcholine group, is fixed on a wall surface including, for example, silanol by ionic interaction, is obtained by flowing a polymer solution including a phospholipid polymer combining, for example, MPC and BMA or MPC and SMA, through a capillary that includes silanol on the wall surface, for example. Consequently, adsorption of phosphorylated compounds can be prevented simply and highly durably. | 04-14-2011 |
| 20110120867 | MICRO-CHANNEL CHIP FOR ELECTROPHORESIS AND METHOD FOR ELECTROPHORESIS - The micro-channel chip for electrophoresis of the present invention comprises a first substrate formed of a gas-permeable material and a second substrate formed of a gas-permeable or a gas-impermeable material, the first and the second substrate being glued together, the mating surface of either one of the first and second substrates having a sample-feeding channel having a port at both ends and an electrophoretic channel also having a port at both ends, the sample-feeding channel and the electrophoretic channel being allowed to communicate with each other via a narrower channel having a smaller cross-sectional area than those two channels. The micro-channel chip for electrophoresis of the present invention requires only one power source to perform electrophoresis and can use samples with minimum waste of their quantity. | 05-26-2011 |
| 20130001082 | DNA SEQUENCING USING MULTIPLE METAL LAYER STRUCTURE WITH ORGANIC COATINGS FORMING TRANSIENT BONDING TO DNA BASES - A technique for nanodevice is provided. A reservoir is filled with an ionic fluid. A membrane separates the reservoir, and the membrane includes electrode layers separated by insulating layers in which the electrode layers have an organic coating. A nanopore is formed through the membrane, and the organic coating on the electrode layers forms transient bonds to a base of a molecule in the nanopore. When a first voltage is applied to the electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels through the transient bonds formed to the base to be measured as a current signature for distinguishing the base. | 01-03-2013 |
| 20130105314 | HIGH SPEED, HIGH RESOLUTION COMPOSITIONS, METHODS AND KITS FOR CAPILLARY ELECTROPHORESIS | 05-02-2013 |
| 20130105317 | Electronic Device for Pathogen Detection | 05-02-2013 |
| 20130105318 | HIGH THROUGHPUT AND VOLUMETRIC ERROR RESILIENT DILUTION WITH DIGITAL MICROFLUIDIC BASED LAB-ON-A-CHIP | 05-02-2013 |
| 20130105319 | ARCHITECTURAL LAYOUT FOR DILUTION WITH REDUCED WASTAGE IN DIGITAL MICROFLUIDIC BASED LAB-ON-A-CHIP | 05-02-2013 |
| 20120125774 | Analysis Chip and Analysis Apparatus - An analysis chip that enables an apparatus to be small, analysis to be simple, analysis time to be short and analysis of both glycosylated hemoglobin and glucose to be highly accurate is provided. The electrophoresis chip includes an upper substrate | 05-24-2012 |
| 20120125773 | Analysis Chip and Analysis Apparatus - An analysis chip that enables an apparatus to be small, analysis to be simple, analysis time to be short and analysis of both glycosylated hemoglobin and glucose to be highly accurate is provided. The electrophoresis chip includes an upper substrate | 05-24-2012 |
| 20110079513 | MICROFLUIDIC SYSTEM AND METHOD FOR ASSEMBLING AND FOR SUBSEQUENTLY CULTIVATING, AND SUBSEQUENT ANALYSIS OF COMPLEX CELL ARRANGEMENTS - A microfluidic system ( | 04-07-2011 |
| 20110048945 | APPARATUS AND METHOD FOR TRAPPING BEAD BASED REAGENTS WITHIN MICROFLUIDIC ANALYSIS SYSTEMS - An on-chip packed reactor bed design is disclosed that allows for an effective exchange of packing materials such as beads at a miniaturized level. Also disclosed is a method of treating a sample within a microfluidic analysis system, comprising; providing a main channel having a trapping zone; providing a slurry of a reagent treated packing material; inducing a flow of said packing material into said trapping zone through a flow channel connected to said trapping zone to load said trapping zone and form a packed bed of said packing material; and flowing a sample containing analytes through said packed bed, said reagent treating the sample. The present invention extends the function of microfluidic analysis systems to new applications including on-chip solid phase extraction (SPE) and on-chip capillary electrochromatography (CEC). The design can be further extended to include integrated packed bed immuno- or enzyme reactors. | 03-03-2011 |
| 20110083961 | MICROFLUIDIC SYSTEM AND CORRESPONDING OPERATING METHOD - The invention relates to an operating method for a microfluidic system, including the following steps: feeding of a carrier flow with particles ( | 04-14-2011 |
| 20130118901 | Apparatuses and Methods for Manipulating Droplets - Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes. | 05-16-2013 |
| 20110100817 | MICROFLUIDIC DEVICES AND METHODS OF USING SAME - The presently-disclosed subject matter provides microfluidic devices comprised of two or more carbon nanotube membranes disposed at predetermined intervals within a microchannel. Further provided are methods of using the same for the electrokinetic separation of one or more molecules of interest from a sample. | 05-05-2011 |
| 20110024292 | COMPOSITIONS AND METHODS FOR CYCLOFRUCTANS AS SEPARATION AGENTS - The present invention relates to derivatized cyclofructan compounds, compositions comprising derivatized cyclofructan compounds, and methods of using compositions comprising derivatized cyclofructan compounds for chromatographic separations of chemical species, including enantiomers. Said compositions may comprise a solid support and/or polymers comprising derivatized cyclofructan compounds. | 02-03-2011 |
| 20100200402 | PRESSURE MANIFOLD TO EQUALIZE PRESSURE IN INTEGRATION PCR-CE MICROFLUIDIC DEVICES - A device is presented that includes a chip having a plurality of wells that are optionally connected by capillary channels, and a manifold member configured to be disposed over the chip for equalizing pressure over the wells and capillary channels. | 08-12-2010 |
| 20100200401 | Polypeptide Markers for the Early Recognition of the Rejection of Transplanted Kidneys - A method for recognizing rejection after a kidney transplantation (NTx), comprising the step of determining the presence or absence of at least one polypeptide marker in a sample, wherein said polypeptide marker is selected from markers 1 to 242 (frequency markers), or determining the amplitude of at least one polypeptide marker selected from markers 243 to 767 (amplitude markers), which are characterized by the values for the molecular masses and migration times (CE time). | 08-12-2010 |
| 20140353157 | DISPOSABLE CARTRIDGE FOR MICROFLUIDICS SYSTEM - A digital microfluidics system for manipulating samples in liquid droplets within a gap between a first hydrophobic surface of a bottom layer and a second hydrophobic surface of at least one disposable cartridge. Disposable cartridges comprise a body and/or a rigid cover plate. The bottom layer of each disposable cartridge is a flexible film that is sealingly attached to the body or plate. The cartridge has no spacer between the first and second hydrophobic surfaces. When using these cartridges, the bottom layers configured as a working film for manipulating samples in liquid droplets thereon, is placed on an electrode array of a digital microfluidics system. The array has individual electrodes. The digital microfluidics system also comprises a central control unit for controlling the selection of the individual electrodes of the electrode array and for providing these electrodes with individual voltage pulses for manipulating liquid droplets by electrowetting. | 12-04-2014 |
| 20100258439 | CAPILLARY ELECTROPHORESIS METHOD FOR ANALYZING COLLAGEN AND AN ASSAY KIT FOR THE SAME - A capillary electrophoresis method for identification and analyzing collagen quantitatively, which is used to identify and quantify collagen in a sample, comprises the steps of: (a) dissolving a collagen-containing sample to form a sample solution; (b) preparing a capillary with an inner wall thereof having a positively-charged layer; (c) introducing the sample solution into the capillary filled with an analytical buffer solution; and (d) driving the sample solution to pass through the capillary. The present invention also discloses an assay kit implementing the abovementioned method. The method of the present invention does not need the purifying pre-treatment and cracking the collagen-containing sample but directly performs the capillary electrophoresis analysis of collagen. Therefore, the present invention can shorten the time for analyzing collagen quantitatively. | 10-14-2010 |
| 20100187111 | Graft Copolymers, Their Preparation And Use in Capillary Electrophoresis - The invention relates to graft copolymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; also to ultra-high molecular weight poly(N,N-dimethylacrylamide) (“poly(DMA)”) polymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; and more particularly to supports, such as capillaries, containing these polymers and methods for separating biomolecules, especially polynucleotides, using capillary electrophoresis. The graft copolymers can be prepared by, e.g., grafting polyacrylamide units onto a poly(DMA) backbone. Separation media comprising such graft copolymers or ultra-high molecular weight poly(DMA) polymers yield superior performance in the analysis and separation of biomolecules by capillary electrophoresis. | 07-29-2010 |
| 20120138461 | Device and Method for Manufacturing the Same - The present invention provides a device that decreases deformation during manufacturing of the device, provides a firm joint without use of an adhesive, and allows chemical modification of a channel during manufacturing of the device. The device includes two joined substrates, and a concavity is formed on at least one of the opposing surfaces of the two substrates so as to make a channel, where the two substrates are joined together by a covalent bond via a crosslinking agent (A), and the crosslinking agent (A) is exposed on an inner wall surface of the channel. | 06-07-2012 |
| 20110127164 | NON-INVASIVE ACOUSTIC TECHNIQUE FOR MIXING AND SEGREGATION OF FLUID SUSPENSIONS IN MICROFLUIDIC APPLICATIONS - The present invention includes an apparatus and corresponding method for fluid flow control in microfluidic applications. A microchamber, filled with a fluid, is in fluid contact with a flexible plate. A transducer is acoustically coupled to the flexible plate. A function generator outputs a signal to excite the transducer, which in turn induces drumhead vibration of the flexible plate, creating a flow pattern within the fluid filled microchamber. | 06-02-2011 |
| 20140027283 | METHOD AND MARKER FOR THE DIAGNOSIS OF A BILE DUCT STRICTURE AND OF A CHOLANGIOCELLULAR CARCINOMA IN BILE - A method for the diagnosis of a benign or malignant bile duct stricture and/or of a CCC, comprising the step of determining at least three polypeptide markers in a body fluid, wherein the polypeptide marker belongs to those markers which in table 1 and/or table 2 a and/or b are characterized by values for the molecular mass and the migration time. | 01-30-2014 |
| 20150075988 | INTEGRATED MICROFLUIDIC CIRCUIT WITH ELECTROWETTING-BASED OPERATION AND CORRESPONDING MICROFLUIDIC SYSTEM - An integrated fluidic circuit has a supporting surface that carries a first fluid to be moved at a first functional region; a dielectric structure, defining the supporting surface; and an electrode structure, coupled to the dielectric structure for generating an electric field at the first functional region, such as to modify electrowetting properties of the interface between the first fluid and the supporting surface. The dielectric structure has a first spatially variable dielectric profile at the first functional region, thus determining a corresponding spatially variable profile of the electric field, and, consequently, of the electrowetting properties of the interface between the first fluid and the supporting surface. The integrated fluidic circuit may achieve mixing between the first fluid and a second fluid. | 03-19-2015 |
| 20110073477 | Matrix and Dynamic Polymer Systems and Compositions for Microchannel Separation - Matrix polymers and dynamic coating polymers, compositions thereof and related methods, systems and apparatus for microchannel separation. | 03-31-2011 |
| 20100294663 | METHODS AND DEVICES FOR ISOTACHOPHORESIS APPLICATIONS - The invention relates to an operation mode of electrophoresis, which separates and/or fractionates particles of differentiated electrophoretic mobility. More specifically, the invention relates to isotachophoresis (ITP), including free-flow and capillary isotachophoresis, and provides novel electrophoresis methods, as well as kits and devices for carrying out such methods. | 11-25-2010 |
| 20160116439 | Systems and Methods for Sample Preparation, Processing and Analysis - The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system. | 04-28-2016 |
| 20160116436 | FREE-FLOW ELECTROPHORESIS DEVICE AND METHOD - There is provided an electrophoresis device. The device includes a chamber, for containing liquid medium, the liquid medium including a flowing separation medium, carrying a sample, a pair of opposing electrodes for generating an electric field, with effect that the generated electric field effects spatial separation of the sample into sample fractions and, with respect to at least one of the electrodes, also effects generation of gaseous material from the liquid medium disposed at, or substantially at, an operative electrode surface of the electrode, with effect that the generated gaseous material becomes, at least initially, disposed within the liquid medium, wherein the generated gaseous material includes at least one generated gaseous compound, and at least one outlet for collecting a sample fraction. In some embodiments, for example, the device further includes a gas separator for inducing removal of at least a fraction of the generated gaseous material from the chamber, the gas separator including a space configured for effecting fluid communication between the liquid medium and a generated gaseous material receiving fluid phase such that a fluid interface is defined between the liquid medium and the generated gaseous material receiving fluid phase, and such that at least a fraction of the generated gaseous material migrates upwardly from its disposition at, or substantially at, the operative electrode surface to the fluid interface in response to buoyancy forces, and then from the fluid interface and into the generated gaseous material receiving fluid phase in response to a driving force. In some embodiments, the device further includes flow guides, disposed between the electrodes, for directing the flowing separation medium towards the at least one outlet. In some implementations, the flow guides mitigate the creation of a pH gradient. | 04-28-2016 |
| 20110036717 | METHOD AND MARKER FOR DIAGNOSIS OF TUBULAR KIDNEY DAMAGE AND ILLNESS - A method for the diagnosis of tubular kidney diseases comprising the step of determining the presence or absence or amplitude of at least three polypeptide markers in a urine sample, the polypeptide marker being selected from the markers characterized in Table 1 by values for the molecular masses and migration times. | 02-17-2011 |
| 20110000787 | High Speed, High Resolution Compositions, Methods and Kits for Capillary Electrophoresis - The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided. | 01-06-2011 |
| 20110162963 | System and Method for Increasing Polymer/Nanopore Interactions - An electrolytic system includes an analyte chamber having an access port for introducing a sample containing a molecules of interest, such as DNA. Electrodes create an electric field along a length of the analyte chamber to drive molecules toward an interaction region containing a nanopore, thereby increasing the arrival rate of molecules at the nanopore. Additional electrodes may be utilized to create an electric field through the nanopore to drive a molecule into the nanopore. A current sensor may be utilized to count, discriminate or characterize the molecules as they interact with the nanopore. Advantageously, system can be utilized for unamplified DNA sequencing. | 07-07-2011 |
| 20100236930 | Electrowetting Dispensing Devices and Related Methods - A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting. | 09-23-2010 |
| 20120118736 | LIQUID DIELECTROPHORETIC DEVICE AND METHOD FOR CONTROLLABLY TRANSPORTING A LIQUID USING THE SAME - A liquid dielectrophoretic device comprises: a first container unit defining a first micro containing space including an electrode pair for generating a dielectrophoretic force; a second container unit defining a second micro containing space and including an electrode pair for generating a dielectrophoretic force; and a fluid channel unit defining a micro-channel between the first and second micro containing spaces and including an electrode pair having a middle region layer that has first and second enlarged sections and a middle section disposed between the first and second enlarged sections. The first and second enlarged sections are enlarged gradually from the middle section to the first and second micro containing spaces. A method for controllably transporting a liquid using the liquid dielectrophoretic device is also disclosed. | 05-17-2012 |
| 20160041124 | APPARATUS AND METHOD FOR EXTRACTING PARTICLES - An apparatus and method for extracting particles are provided. The particle extraction apparatus includes: a main body; a support unit provided in the main body and in which a sample plate is disposed; an extraction unit that can be selectively submerged in a sample of the sample plate and that extracts particles within the sample; a signal generator that generates an electrical signal so as to generate an electrical field within the sample within the sample plate; and a controller that controls operation of the signal generator, wherein particles within the sample are attached to the extraction unit to be extracted by an electrical field generated within the sample. | 02-11-2016 |
| 20150377830 | DETECTION OF TRANSLOCATION EVENTS USING GRAPHENE-BASED NANOPORE ASSEMBLIES - Translocation events are sensed using composite nanopore assemblies including nanopores formed in graphene sheets. Single molecule detection and characterization and multi-molecule characterization and identification are provided using such assemblies. Multiple electrodes associated with nanofluidic sensors facilitate detection of ionic current through a nanopore as well as tunneling currents. Current signals of individual molecules are estimated from the combination of an ionic current signal through the nanopore and tunneling current signals obtained at specific locations within the nanopore. | 12-31-2015 |
| 20130334046 | NANOPORE DEVICE WETTING - A method for wetting a nanopore device includes filling a first cavity of the nanopore device with a first buffer solution having a first potential hydrogen (pH) value, filling a second cavity of the nanopore device with a second buffer solution having a second pH value, wherein the nanopore device includes a transistor portion having a first surface, an opposing second surface, and an orifice communicative with the first surface and the second surface, the first surface partially defining the first cavity, the second surface partially defining the second cavity, applying a voltage in the nanopore device, and measuring a current in the nanopore device, the current having a current path partially defined by the first cavity, the second cavity, and the orifice. | 12-19-2013 |
| 20130306476 | NANOWIRE DEVICE FOR MANIPULATING CHARGED MOLECULES - The invention relates to a nanowire device for manipulation of charged molecules, comprising a tubular nanowire with a through-going channel; a plurality of individually addressable wrap gate electrodes arranged around said tubular nanowire with a spacing between each two adjacent wrap gate electrodes and means for connecting the wrap gate electrodes to a voltage source. The invention further relates to a nanowire system comprising at least one nanowire device, and to a method for manipulating of charged molecules within a through-going channel of a tubular nanowire. | 11-21-2013 |
| 20100314250 | STABILIZATION OF COMPLEXES FOR GEL MOBILITY SHIFT, CHROMATOGRAPHY, AND CAPILLARY ELECTROPHORESIS - The invention provides a method of separating a complex comprising a first agent bound to a second agent from unbound agents comprising (a) preparing a gel comprising a gel matrix and a neutral solute, (b) placing one or more samples comprising the complex and unbound agents on the gel, and (c) applying an electrophoresis voltage across the gel and separating the complex from the unbound agents. The invention also provides methods of separating a complex comprising a first agent bound to a second agent from unbound agents by chromatography and capillary electrophoresis. | 12-16-2010 |
| 20130240359 | NANOPORE SEQUENCING USING CHARGE BLOCKADE LABELS - The invention relates to devices and methods for nanopore sequencing. The invention includes compositions and methods of nucleic acid sequencing using a single polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid attached proximal to a nanopore, and nucleotide analogs in solution comprising charge blockade label that are attached to the polyphosphate portion of the nucleotide analog such that the charge blockade labels are cleaved when the nucleotide analog is incorporated into a growing nucleic acid and the charge blockade label is detected by the nanopore to determine the presence and identity of the incorporated nucleotide and thereby determine the sequence of a template nucleic acid. | 09-19-2013 |
| 20130240358 | Surfactant-Based Monolithic Columns, Methods for Making the Same, and Methods for Using the Same - A method for making a surfactant-based monolithic column is provided. The method comprises providing a mixture comprising at least one surfactant monomer, at least one crosslinker, at least one initiator, and at least one porogen and polymerizing the mixture to form the surfactant-based monolithic column. The present disclosure also provides a surfactant-based monolithic column, a method for separating molecules, and a process for preparing a surfactant monomer. | 09-19-2013 |
| 20130240357 | METHODS AND DEVICES FOR SINGLE-MOLECULE WHOLE GENOME ANALYSIS - Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals. | 09-19-2013 |
| 20130240356 | NANOPORES IN ZERO MODE WAVEGUIDES - Methods, devices, substrates, and systems are disclosed involving arrays of zero-mode waveguides having nanopores extending through the bases that form the bottoms of the zero-mode-waveguides. Electric fields across the nanopores are used to attach single biomolecules such as polymerase enzymes within each zero-mode-waveguide. Electric fields across the nanopores can also be used for the active loading of nucleic acid templates into enzymes attached within the zero mode waveguides. | 09-19-2013 |
| 20130240355 | FUNCTIONALIZATION OF GRAPHENE HOLES FOR DEIONIZATION - A method for deionization of a solution, the method comprising functionalizing plural apertures of a graphene sheet to repel first ions in the solution from transiting through the functionalized plural apertures. The non-transiting first ions influence second ions in the solution to not transit through the functionalized plural apertures. The graphene sheet is positioned between a solution flow path input and a solution flow path output. Solution enters the solution flow path input and through the functionalized plural apertures of the graphene sheet, resulting in a deionized solution on the solution flow path output side of the graphene sheet and a second solution containing the first ions and second ions on the solution flow path input side of the graphene sheet. | 09-19-2013 |
| 20150107996 | NON-FARADAIC, CAPACITIVELY COUPLED MEASUREMENT IN A NANOPORE CELL ARRAY - A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current. | 04-23-2015 |
| 20140353158 | DEVICE AND METHOD FOR CONTROLLED ADHESION UPON MOIST SUBSTRATE - A device for generating an adjustable adhesion force on a wet substrate is described. The device has a main body with an adhesion surface which, in use, arranges itself facing the substrate, at such adhesion surface the main body having a plurality of channels generating a capillary return for water present on the substrate. A delivery and/or reservoir system for silicone oil, providing the latter at the adhesion surface, so that silicone oil arranges itself interposed between the surface itself and the water on the substrate. A static electric field generating system generates a static electric field at the adhesion surface. Such electric field modifies the wettability of silicone oil with respect to the adhesion surface. | 12-04-2014 |
| 20100258441 | Manipulation of Beads in Droplets and Methods for Splitting Droplets - The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered. | 10-14-2010 |
| 20100258440 | ANALYSIS APPARATUS AND ANALYSIS METHOD FOR CAPILLARY ELECTROPHORESIS - A capillary electrophoresis analysis apparatus is provided for analyzing samples by a capillary electrophoresis method that allows for rapid and highly accurate separation and detection, wherein the apparatus may be used in the diagnosis and/or monitoring of selected diseases. | 10-14-2010 |
| 20110220499 | Non-focusing tracers for indirect detection in electrophoretic displacement techniques - A novel method for visualizing electrokinetic process zones (e.g., for isotachophoresis (ITP)) is provided. We introduce negligibly small concentrations of a fluorophore that is not focused by isotachophoresis. This non-focusing tracer (NFT) migrates through multiple isotachophoresis zones. As it enters each zone, the NFT concentration adapts to the local electric field in each zone. ITP zones can then be visualized with a point detector or camera. The method can be used to detect, identify, and quantify unknown analyte zones, and can visualize complex and even transient electrophoresis processes. This visualization technique is particularly suited to microfluidic and lab-on-a-chip applications, as typical fluorescence microscopes and CCD cameras can provide high-resolution spatiotemporal data. | 09-15-2011 |
| 20110220498 | Method for Building Massively-Parallel Preconcentration Device for Multiplexed, High-Throughput Applications - A multiplexed concentration interface that can connect with a plurality of microchannels, conventional 96 well plates or other microarrays is disclosed. The interface can be used in biosensing platforms and can be designed to detect single or multiple targets such as DNA/RNA, proteins and carbohydrates/oligosaccharides. The multiplexed concentration device will provide a set of volume-matched sample preparation and detection strategies directly applicable by ordinary researchers. Furthermore, a multiplexed microfluidic concentrator without buffer channels is disclosed. | 09-15-2011 |
| 20100326826 | APPARATUS AND METHOD FOR TRAPPING BEAD BASED REAGENTS WITHIN MICROFLUIDIC ANALYSIS SYSTEMS - An on-chip packed reactor bed design is disclosed that allows for an effective exchange of packing materials such as beads at a miniaturized level. Also disclosed is a method of treating a sample within a microfluidic analysis system, comprising; providing a main channel having a trapping zone; providing a slurry of a reagent treated packing material; inducing a flow of said packing material into said trapping zone through a flow channel connected to said trapping zone to load said trapping zone and form a packed bed of said packing material; and flowing a sample containing analytes through said packed bed, said reagent treating the sample. The present invention extends the function of microfluidic analysis systems to new applications including on-chip solid phase extraction (SPE) and on-chip capillary electrochromatography (CEC). The design can be further extended to include integrated packed bed immuno- or enzyme reactors. | 12-30-2010 |
| 20150122650 | POLYPEPTIDE MARKERS FOR THE DIAGNOSIS OF BLADDER CANCER - A method for the diagnosis of bladder cancer (BC) and/or for determining a tumor stage of bladder cancer, comprising the step of determining the presence or absence or amplitude of at least six polypeptide markers in a sample, wherein said polypeptide markers are selected from markers 1 to 836, which are characterized by the values for the molecular masses and migration times (CE time). | 05-07-2015 |
| 20140216933 | HIGH CONTRAST SIGNAL TO NOISE RATIO DEVICE COMPONENTS - Provided are device components, devices and methods characterized by a high contrast signal to noise ratio (CNR). | 08-07-2014 |
| 20140061048 | NANOPORE SEQUENCING USING N-MERS - The invention relates to devices and methods for nanopore sequencing. The invention provides for using the signals from n-mers to provide sequence information, for example where the system has less than single base resolution. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed. | 03-06-2014 |
| 20140318962 | NANOPORE DEVICE FOR DRUG-LIKE MOLECULE SCREENING OR LEAD OPTIMIZATION TO A TARGETED PROTEIN - A nanosensor for detecting molecule characteristics includes a membrane having an opening configured to permit a charged molecule to pass but to block a protein molecule attached to a ligand connecting to the charged molecule, the opening being filled with an electrolytic solution. An electric field generator is configured to generate an electric field relative to the opening to drive the charged molecule through the opening. A sensor circuit is coupled to the electric field generator to sense current changes due to charged molecules passing into the opening. The current changes are employed to trigger a bias field increase to cause separation between the ligand and the protein to infer an interaction strength. | 10-30-2014 |
| 20100213062 | Disease detection system and method - A disease detection system which includes an electrophoresis apparatus and a biomarker detector. The apparatus includes: (a) a transport passage; (b) a plurality of separation passages, each of the separation passages having an overlapping portion that overlaps the transport passage; and (c) a different biomarker concentrator in each of the overlapping portions and which concentrates at least one different biomarker from a specimen from an animal introduced into the transport passage. A plurality of the different biomarkers is associated with at least one predetermined disease, such as diabetes, heart disease or cancer. The detector detects the presence of the different biomarkers which were concentrated by the biomarker concentrators and delivered to the biomarker detector via the separation passages. First and second sets of the biomarker concentrators can be associated with respective first and second predetermined diseases and the user can determine which set will be used in each procedure. | 08-26-2010 |
| 20140202857 | DEVICE AND SINGLE-MOLECULE ANALYSIS METHOD BY MEANS OF DETECTION OF THE COLLISIONS OF A TARGET MOLECULE ON FUNCTIONALIZED NANOPORES - The present invention concerns a device and a method of single-molecule analysis by detection of a target molecule on functionalized nanopores in such a way that it interacts with the target molecule and has an effective diameter smaller than the dimension of the target molecule. | 07-24-2014 |
| 20140251808 | METHOD AND SYSTEM FOR CONCENTRATING PARTICLES FROM A SOLUTION - Methods and systems are provided for concentrating particles (e.g., bacteria, viruses, cells, and nucleic acids) suspended in a liquid. Electric-field-induced forces urge the particles towards a first electrode immersed in the liquid. When the particles are in close proximity to (e.g., in contact with) the first electrode, the electrode is withdrawn from the liquid and capillary forces formed between the withdrawing electrode and the surface of the liquid immobilize the particles on the electrode. Upon withdrawal of the electrode from the liquid, the portion of the electrode previously immersed in the liquid has particles immobilized on its surface. | 09-11-2014 |
| 20130008789 | METHOD AND APPARATUS USING ELECTRIC FIELD FOR IMPROVED BIOLOGICAL ASSAYS - Disclosed are a method and apparatus that use an electric field for improved biological assays. The electric field is applied across a device having wells, which receive reactants, which carry a charge. The device thus uses a controllable voltage source between the first and second electrodes, which is controllable to provide a positive charge and a negative charge to a given electrode. By controlled use of the electric field charged species in a fluid in a fluid channel are directed into or out of the well by an electric field between the electrodes. The present method involves the transport of fluids, as in a microfluidic device, and the electric field-induced movement of reactive species according to various assay procedures, such as DNA sequencing, synthesis or the like. | 01-10-2013 |
| 20110147216 | MICROFLUIDIC SYSTEM AND METHOD FOR CREATING AN ENCAPSULATED DROPLET WITH A REMOVABLE SHELL - A microfluidic system for creating encapsulated droplets whose shells can be further removed comprises: two electrode plates and a spacing structure disposed between the two electrode plates. One of the electrode plates has three reservoir electrodes and a plurality of channel electrodes. The three electrodes are respectively used for accommodating a shell liquid, a core liquid, and a removing liquid which is able to remove the shell liquid. The channel electrodes are used for communicating droplets among the three reservoir electrodes. Via these arrangements, the microfluidic system can create a quantitative shell droplet and a quantitative core droplet, and then merge the shell and core droplets to form an encapsulated droplet. Moreover, the shell of the encapsulated droplet can be removed by mixing it with the removing liquid. This invention is further provided with a method for creating an encapsulated droplet with a removable shell. | 06-23-2011 |
| 20120217161 | Methods and Products for Analyzing Polymers - Methods and products for analyzing polymers are provided. The methods include methods for determining various other structural properties of the polymers. | 08-30-2012 |
| 20140318963 | METHODS FOR IONOPHORICALLY SCREENING PORE FORMING BACTERIAL PROTEIN TOXINS AND RECEPTORS - One embodiment of the present invention is directed to methods for ionophorically screening pore forming bacterial protein toxins and receptors. The method includes: a) forming a membrane comprising a lipid and a receptor, b) contacting the membrane with the pore forming bacterial protein toxin and an ion solution, and c) measuring ion flow through the membrane. | 10-30-2014 |
| 20140360877 | DEVICES WITH FLUIDIC NANOFUNNELS, ASSOCIATED METHODS, FABRICATION AND ANALYSIS SYSTEMS - Methods of forming a chip with fluidic channels include forming (e.g., milling) at least one nanofunnel with a wide end and a narrow end into a planar substrate, the nanofunnel having a length, with width and depth dimensions that both vary over its length and forming (e.g., milling) at least one nanochannel into the planar substrate at an interface adjacent the narrow end of the nanofunnel. | 12-11-2014 |
| 20100065427 | MICROFLUIDIC CHIP AND ANALYTICAL METHOD - A microfluidic chip includes: a channel which is provided on a first portion of a surface of a substrate part, the channel being used for separating a substance subject to analysis by electrophoresis and adding a reaction reagent with a nozzle to the substance separated by electrophoresis; and an absorption region which is provided on a second portion of the surface of the substrate part, and which absorbs the reaction reagent, the second portion being different from the first portion. | 03-18-2010 |
| 20100032294 | METHOD FOR ANALYZING HEMOGLOBIN BY CAPILLARY ELECTROPHORESIS AND ADDITIVE USED THEREIN - The present invention provides a method for analyzing hemoglobin by capillary electrophoresis, that allows the apparatus to be smaller in size, allows a highly precise analysis to be obtained, and allows the analysis to be performed in a short period of time. The analytical method of the present invention are methods for analyzing hemoglobin by capillary electrophoresis, comprising: a sample-providing step of providing a sample containing hemoglobin; a capillary tube-providing step of providing a capillary tube containing a buffer solution; and an electrophoresis step of carrying out electrophoresis of the sample, by introducing the sample into the buffer solution in the capillary tube, and applying a voltage across both ends of the capillary tube; wherein the electrophoresis is carried out following at least one of modes (A) and (B) below: (A) the electrophoresis is carried out with a surfactant (a) added to the buffer solution, the surfactant (a) being a non-ionic surfactant having an alkyl group as a hydrophobic portion and a sugar as a hydrophilic portion; and (B) the electrophoresis is carried out with a surfactant (b) added to the sample, the surfactant (b) being a betaine-type amphoteric surfactant. | 02-11-2010 |
| 20100187110 | PROCESS FOR ANALYZING SAMPLE BY CAPILLARY ELECTROPHORESIS METHOD - A process for analyzing a sample by a capillary electrophoresis method is provided that allows the apparatus to be reduced in size, allows a high analytical precision to be obtained, and can be carried out easily. The analytical process of the present invention is a process for analyzing a sample by a capillary electrophoresis method. The analytical process includes preparing a capillary tube to be used for the capillary electrophoresis method, and performing electrophoretic separation of a complex of a sample and an anionic group-containing compound that are bonded together, in the capillary tube, wherein the capillary tube includes an anionic layer that is formed of the anionic group-containing compound and that is coated on the inner wall of the capillary tube, and the anionic layer is fixed to the inner wall of the capillary tube by a covalent bond. | 07-29-2010 |
| 20100155241 | GRADIENT ELUTION ELECTROPHORESIS - A method for performing electrophoretic separation of ionic compounds which involves varying a bulk fluid flow though a separation path into which ionic species are continuously introduced and separated. The method can also include the introduction of a leading electrolyte into the separation path to form an ionic interface with the sample and an optional terminating electrolyte to enrich ionic species for higher detection resolution. | 06-24-2010 |
| 20100122907 | SINGLE MOLECULE MASS OR SIZE SPECTROMETRY IN SOLUTION USING A SOLITARY NANOPORE - A nanopore conductance measurement method and system is provided. The system has reservoirs of conductive fluid separated by a resistive barrier, which is perforated by a single nanometer scale pore commensurate in size with an analyte molecule in at least one of the reservoirs. The system is configured to have an ionic current driven across the reservoirs by an applied potential and the pore may be treated so that the pore surface can form associations with the analyte molecules of interest to increase the analyte molecule residence times on or in the pore. The system also comprises a means of measuring the ionic current, which current may be either direct or alternating in time, induced by an applied potential between electrodes in the conductive fluid, on each side of the barrier. The system also comprises a means of recording the ionic current time course as a time series, which includes time periods when the pore is unobstructed and also in periods when analyte molecules cause pulses of reduced conductance. The method comprises methods to delineate segments of the conductance time series into regions statistically consistent with the unobstructed pore conductance level, and pulses of reduced conductance, and also statistically stationary segments within individual pulses of reduced conductance. The method may also provide steps for interpreting the statistical analysis to yield parameters such as size, mass, and/or concentration of at least one type of analyte in solution. | 05-20-2010 |
| 20150346150 | AUTOSOMAL-DOMINANT POLYCYSTIC KIDNEY DISEASE (ADPKD) - The process for the diagnosis, early detection and prognosis of the clinical development of autosomal-dominant polycystic kidney disease (ADPKD) comprises the step of determining the presence or absence or amplitude of at least three polypeptide markers in a urine sample, the polypeptide markers being selected from the markers characterized in Table 1 by values for the molecular masses and migration times. | 12-03-2015 |
| 20140209461 | Poly and Copoly(N-vinylamide)s and Their Use In Capillary Electrophoresis - The invention relates generally to polymers and copolymers comprising N-vinylamide-type monomers, their preparation, and compositions, such as electrophoresis separation media, containing the same; to supports, such as capillaries, containing these polymers; and methods for separating a mixture of biomolecules, especially polynucleotides, using capillary electrophoresis. Separation media comprising such polymers yield advantageous performance in the analysis and separation of biomolecules by capillary electrophoresis. | 07-31-2014 |
| 20100155242 | Method of Analyzing a Sample by Capillary Electrophoresis - The present invention is directed to the described capillary electrophoresis apparatus and methods of using such apparatus for separating and analyzing components of a sample. | 06-24-2010 |
| 20130284598 | METHODS AND APPARATUSES FOR POSITIONING NANO-OBJECTS WITH ASPECT RATIOS - A method for positioning nano-objects on a surface and an apparatus for implementing the method. The method includes: providing a first surface and a second surface in a position facing each other, where one or more of the surfaces exhibits one or more position structures having dimensions on the nanoscale; providing an ionic liquid suspension of the nano-objects between the two surfaces, where the suspension comprises two electrical double layers each formed at an interface with a respective one of the two surfaces, and the surfaces have electrical charges of the same sign; enabling the nano-objects in the suspension to position according to a potential energy resulting from the electrical charge of the two surfaces; and depositing one or more of the nano-objects on the first surface according to the positioning structures by shifting the minima of the potential energy towards the first surface. | 10-31-2013 |
| 20150060275 | DNA SEQUENCING USING A SUSPENDED CARBON NANOTUBE - A technique is provided for forming a nanodevice for sequencing. A bottom metal contact is disposed at a location in an insulator that is on a substrate. A nonconducting material is disposed on top of the bottom metal contact and the insulator. A carbon nanotube is disposed on top of the nonconducting material. Top metal contacts are disposed on top of the carbon nanotube at the location of the bottom metal contact, where the top metal contacts are formed at opposing ends of the carbon nanotube at the location. The carbon nanotube is suspended over the bottom metal contact at the location, by etching away the nonconducting material under the carbon nanotube to expose the bottom metal contact as a bottom of a trench, while leaving the nonconducting material immediately under the top metal contacts as walls of the trench. | 03-05-2015 |
| 20140291153 | NANOPORE FUNCTIONALITY CONTROL - A method is provided of controlling the functionality of a substrate containing at least one nanopore. The method includes the steps of: introducing to the substrate a solution containing a molecular construct having a body formation which defines an aperture and a tail formation extending from the body formation; applying a potential difference across the substrate to thread the tail formation through the nanopore thereby docking the molecular construct to the substrate with the aperture aligned with the nanopore such that the sleeve formation lines the nanopore; and expelling the molecular construct from the substrate by varying the potential difference. A DNA construct for docking to a substrate having a nanopore is also provided, the construct having a body formation which defines an aperture, and a tail formation extending from the body formation for threading through the nanopore to dock the construct to the substrate with the aperture and nanopore in alignment. | 10-02-2014 |
| 20110240471 | EXCHANGEABLE CARRIERS PRE-LOADED WITH REAGENT DEPOTS FOR DIGITAL MICROFLUIDICS - The present invention provides exchangeable, reagent pre-loaded carriers ( | 10-06-2011 |
| 20130264205 | METHOD OF PROCESSING TARGET MATERIAL IN A SAMPLE - An efficient method of processing a target material in a sample using a microfluidic device including an elastic membrane. | 10-10-2013 |
| 20130264204 | METHOD AND APPARATUS FOR DETECTING NUCLEOTIDES - A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer. | 10-10-2013 |
| 20100116657 | METHOD AND APPARATUS FOR CONCENTRATING MOLECULES - A method and apparatus for continuously separating or concentrating molecules that includes flowing two fluids in laminar flow through an electrical field and capturing at one of three outputs a fluid stream having a different concentration of molecules. | 05-13-2010 |
| 20100101953 | Methods for producing microchannel chips, microchannel chips, methods for separating biomolecules using the microchannel chips, and electrophoretic apparatus having the microchannel chips - The methods for producing microchannel chips of the present invention comprise the steps of shielding substrate surfaces on which groove-like channels have been formed, using a mask that exposes the channels, and then forming polymer membranes on the exposed surfaces of the substrates; and the step of laminating cover materials onto the side of the substrate surfaces on which the channels have been formed. | 04-29-2010 |
| 20140374253 | SYSTEMS AND METHODS FOR MANIPULATING A MOLECULE IN A NANOPORE - Techniques for manipulating a molecule in a nanopore embedded in a lipid bilayer are described. In one example, an acquiring electrical stimulus level is applied across a lipid bilayer wherein a region of the lipid bilayer containing the nanopore is characterized by a resistance and wherein the acquiring electrical stimulus level tends to draw the molecule from a surrounding fluid into the nanopore, a change in the resistance of the lipid bilayer resulting from the acquisition of at least a portion of a molecule into the nanopore is detected, the acquiring electrical stimulus level is changed to a holding electrical stimulus level wherein the portion of the molecule remains in the nanopore upon the changing of the acquiring electrical stimulus level to the holding electrical stimulus level. | 12-25-2014 |
| 20130140180 | SHEATH-FLOW ELECTROSPRAY INTERFACE - A sheath-flow interface for producing electrospray from a capillary is provided. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. In the interface, electrokinetic flow moves a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The mixture of sheath liquid and analyte is directed to an electrospray emitter in order to generate an electrospray. | 06-06-2013 |
| 20120132528 | Methods of Dispensing and Withdrawing Liquid in an Electrowetting Device - The invention provides a method for dispensing liquid, comprising the steps of: (a) positioning a droplet to be dispensed in a gap of an electrowetting device using an electrowetting array; and (b) dispensing the droplet through a hole in a housing or substrate of the electrowetting device. The invention further provides a method for withdrawing liquid comprising the steps of: (a) positioning a droplet to be withdrawn from a gap of an electrowetting device using an electrowetting array; and (b) withdrawing the droplet through a hole in a housing or substrate of the electrowetting device. | 05-31-2012 |
| 20140332382 | Two-Dimensional Microfluidic Devices and Methods of Using the Same - Microfluidic devices and methods for using the same are provided. Embodiments include microfluidic devices that have a first separation region configured to separate a sample along a first directional axis based on a first property, and a second separation region in fluid communication with the first separation region and configured to separate the sample along a second directional axis based on a second property. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays. | 11-13-2014 |
| 20100175996 | PROCESS FOR ANALYZING SAMPLE BY CAPILLARY ELECTROPHORESIS METHOD - A process for analyzing a sample by a capillary electrophoresis method is provided that allows the apparatus to be reduced in size, allows a high analytical precision to be obtained, and can be carried out easily. The analytical process of the present invention is a process for analyzing a sample by a capillary electrophoresis method. The process includes a step of preparing a capillary channel to be used for the capillary electrophoresis method and a step of electrophoresing a complex of a sample and an anionic group-containing compound that are bonded together, in the capillary channel, wherein the capillary channel includes an A layer that is coated on an inner wall thereof and a B layer that is coated on the A layer. | 07-15-2010 |
| 20110308949 | NANO-FLUIDIC FIELD EFFECTIVE DEVICE TO CONTROL DNA TRANSPORT THROUGH THE SAME - The present invention provides a nano-fluidic field effective device. The device includes a channel having a first side and a second side, a first set of electrodes adjacent to the first side, a second set of electrodes adjacent to the second side, a control unit for applying electric potentials to the electrodes and a fluid within the channel containing a charge molecule. The first set of electrodes is disposed such that application of electric potentials produces a spatially varying electric field that confines a charged molecule within a predetermined area of said channel. The second set of electrodes is disposed such that application of electric potentials relative to the electric potentials applied to the first set of electrodes creates an electric field that confines the charged molecule to an area away from the second side of the channel. | 12-22-2011 |
| 20130043130 | AMMUNITION AND WEAPON TYPE IDENTIFICATION BASED ON GUNSHOT RESIDUE ANALYSIS - The present invention relates to a method of identifying ammunition type and/or weapon type used to fire the ammunition from gunshot residue. This method involves providing a sample including a gunshot residue, subjecting the sample to spectroscopic analysis to produce a spectroscopic signature for the sample, and identifying the spectroscopic signature to ascertain the type of ammunition and/or the type of weapon used to fire the ammunition. A method of establishing reference spectroscopic signatures for ammunition type and/or weapon type used to fire the ammunition is also disclosed. | 02-21-2013 |
| 20120000778 | Method for Modifying the Concentration of Reactants in a Microfluidic Device - A method of modifying the concentration of reactants and carrying out a chemical reaction on a microfluidic device in which first and second reactants are delivered into a reaction channel combined, the second reactant different from the first reactant and capable of reacting with the first reactant. The first reactant is subjected to a stacking process, thereby producing a first stacked reactant. The second reactant is subjected to the stacking process, thereby producing a second stacked reactant. The first stacked reactant is exposed to the second stacked reactant so that the first stacked reactant and the second stacked reactant undergo a chemical reaction. | 01-05-2012 |
| 20120000777 | DEVICES AND METHODS FOR FORMING DOUBLE EMULSION DROPLET COMPOSITIONS AND POLYMER PARTICLES - The present invention generally relates to double emulsion droplet compositions, polymer particles that can be formed from such double emulsion droplet compositions, and to methods and apparatuses for making such compositions and particles. A double emulsion generally describes larger droplets that contain smaller droplets therein. These double emulsion droplet compositions can be used to create a variety of materials including polymer particles and polymeric shells and are further useful for encapsulating a variety of species including catalyst compounds and pharmaceutical agents. The double emulsion droplet compositions disclosed herein are readily formed using planar droplet (“digital”) microfluidic devices without channels, and either air or an immiscible liquid as an ambient medium. | 01-05-2012 |
| 20130068618 | CHARGED ENTITIES AS LOCOMOTIVE TO CONTROL MOTION OF POLYMERS THROUGH A NANOCHANNEL - A technique for controlling the motion of one or more charged entities linked to a polymer through a nanochannel is provided. A first reservoir and a second reservoir are connected by the nanochannel. An array of electrodes is positioned along the nanochannel, where fluid fills the first reservoir, the second reservoir, and the nanochannel. A first electrode is in the first reservoir and a second electrode is in the second reservoir. The first and second electrodes are configured to direct the one or more charged entities linked to the polymer into the nanochannel. An array of electrodes is configured to trap the one or more charged entities in the nanochannel responsive to being controlled for trapping. The array of electrodes is configured to move the one or more charged entities along the nanochannel responsive to being controlled for moving. | 03-21-2013 |
| 20130081945 | DEVICE AND METHOD OF MANIPULATING PARTICLES - Provided is a device and method of manipulating particles. The device includes: a channel for accommodating an electrolyte solution including particles to be manipulated; an anode and cathode for imposing a direct current (DC) electric field on the channel; metal strip(s) attached to an inner wall of the channel and resulting in induced-charge electroosmosis near a surface of the channel; a DC power supply unit for supplying a DC voltage to the anode and the cathode of the channel; control electrodes on both sides of the metal strip(s) to locally tune the induced-charge electroosmosis on the metal strip(s) regardless of the global electric field across the channel; and a DC power supply unit for supplying a DC voltage to the control electrodes. | 04-04-2013 |
| 20130134039 | DIGITAL MICROFLUIDICS SYSTEM WITH DISPOSABLE CARTRIDGES - A digital microfluidics system manipulates samples in liquid droplets within disposable cartridges and has disposable cartridges each with a bottom layer, a top layer and a gap therebetween. A base unit with cartridge accommodation sites and at least one electrode array with electrodes works with a cover plate at the sites and a control unit for controlling selection of the electrodes and for providing them with voltage pulses for manipulating liquid droplets within the cartridges by electrowetting. The cover plate has an electrically conductive material that extends parallel to the array. A selection of disposable cartridges and a method for manipulating samples in liquid droplets that adhere to a hydrophobic surface can be used with the system. | 05-30-2013 |
| 20130068617 | CHARGED ENTITIES AS LOCOMOTIVE TO CONTROL MOTION OF POLYMERS THROUGH A NANOCHANNEL - A technique for controlling the motion of one or more charged entities linked to a polymer through a nanochannel is provided. A first reservoir and a second reservoir are connected by the nanochannel. An array of electrodes is positioned along the nanochannel, where fluid fills the first reservoir, the second reservoir, and the nanochannel. A first electrode is in the first reservoir and a second electrode is in the second reservoir. The first and second electrodes are configured to direct the one or more charged entities linked to the polymer into the nanochannel. An array of electrodes is configured to trap the one or more charged entities in the nanochannel responsive to being controlled for trapping. The array of electrodes is configured to move the one or more charged entities along the nanochannel responsive to being controlled for moving. | 03-21-2013 |
| 20130068619 | Method and Apparatus for Detecting Nucleotides - A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer. | 03-21-2013 |
| 20150068901 | Nanofluidic Device for Charge Analysis of Straightened Molecules - This disclosure provides, among other things, a nanofluidic device sensing device is provided. In certain embodiments, the device contains: a) a channel comprising a floor and a ceiling, b) an array of charge sensors in the floor and/or ceiling of the channel, arranged along the longitudinal axis of the channel; c) a capture area in the floor and/or ceiling of the channel at the entrance end of the channel; and d) a first electrode and a second electrode, wherein the first and second electrodes are positioned to provide an electrophoretic force along the longitudinal axis of the channel. Other embodiments, e.g., methods, are also described. | 03-12-2015 |
| 20100264028 | METHOD FOR MULTIPLEXED CAPILLARY ELECTROPHORESIS SIGNAL CROSS-TALK CORRECTION - The present invention provides a simple method to correct cross-talk, after the data have been generated. Adjacent signals are simply subtracted from the original observed signal with a repeating process. The data processing is stopped when a predefined condition is met. By this technique, cross-talk can be reduced from >5% to less than 0.1%. And as an additional advantage, this method provides a way to correct the cross-talk without the need to know which peaks are caused by the adjacent capillary signal. | 10-21-2010 |
| 20140318965 | MACROMOLECULE POSITIONING BY ELECTRICAL POTENTIAL - Provided herein is technology relating to depositing and/or placing a macromolecule at a desired site for an assay and particularly, but not exclusively, to methods and systems for placing or guiding a macromolecule such as a protein, a nucleic acid, or a protein:nucleic acid complex to an assay site, such as near a nanopore, a nanowell, or a zero mode waveguide. | 10-30-2014 |
| 20140318964 | Two-Chamber Dual-Pore Device - Provided is a device comprising a channel through and defined by a plurality of layers surrounding the channel, the channel connecting a first and a second chambers separated by the plurality of layers, wherein the plurality of layers comprise a first layer, a second layer; and a conductive middle layer disposed between the first and second layers, wherein the channel comprises (a) a first region defined by the first layer, denoted as an inlet, that is about 0.5 nm to about 100 nm in diameter and (b) a second region defined by the second layer, denoted as an outlet, wherein the inlet and the outlet are about 10 nm to about 1000 nm apart from each other, and wherein the first and second chambers and the middle layer are connected to a power supply. Also provided are methods of preparing and using the device, in particular for nucleic acid sequencing. | 10-30-2014 |
| 20160123925 | MULTI-DIMENSIONAL ELECTROPHORESIS APPARATUS - An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest. | 05-05-2016 |
| 20130220810 | THREE-DIMENSIONAL DIGITAL MICROFLUIDIC SYSTEM - A three-dimensional digital microfluidic system comprises a first plate with a first electrode, a second plate with a second electrode, and a microfluidic drop in between the first and the second electrode. The electrodes are able to be actuated in sequence such that the microfluidic drop is able to be transported. A bridge plate is able to be included. | 08-29-2013 |
| 20110048946 | METHOD FOR FINGER-PRINTING HEPARINS - Methods to generate a distinctive fingerprint (pattern of migration) for a sample of complex, polydisperse heparins are provided. The methods involve adding resolving agents such as polyamines to a heparin sample and then analyzing the sample with a technique that separates macromolecules according to charge to mass ratio (e.g. capillary electrophoresis). The resulting electropherogram is unique to and characteristic of the heparin sample. The methods may be used, for example, to monitor the quality and consistency of various heparin preparations. | 03-03-2011 |
| 20140166483 | ELECTROKINETICS-ASSISTED SENSOR - An electrokinetics-assisted sensor for sensing a target material. The sensor may include a microstructure deflectable in response to added mass on its body. The sensor may also include one or more features on or near the microstructure designed to generate an electric field giving rise to one or more electrokinetic effects to drive material towards the microstructure, when an electrical signal is applied to the feature(s). Presence of the target material on the body of the microstructure may cause a response in the microstructure, including a detectable change in deflection of the microstructure. | 06-19-2014 |
| 20150068902 | NANO-FLUIDIC FIELD EFFECTIVE DEVICE TO CONTROL DNA TRANSPORT THROUGH THE SAME - The present invention provides a nano-fluidic field effective device. The device includes a channel having a first side and a second side, a first set of electrodes adjacent to the first side, a second set of electrodes adjacent to the second side, a control unit for applying electric potentials to the electrodes and a fluid within the channel containing a charge molecule. The first set of electrodes is disposed such that application of electric potentials produces a spatially varying electric field that confines a charged molecule within a predetermined area of said channel. The second set of electrodes is disposed such that application of electric potentials relative to the electric potentials applied to the first set of electrodes creates an electric field that confines the charged molecule to an area away from the second side of the channel. | 03-12-2015 |
| 20120031758 | Nanofabricated Structures for Electric Field-Assisted Nucleic Acid Extraction - Embodiments of the invention provide devices and methods for extracting nucleic acid molecules from solution using electric fields. The structures and methods of embodiments of the invention are suited to incorporation into micro and nano fluidic devices, such as lab-on-a-chip devices and micro total analysis systems. | 02-09-2012 |
| 20150075987 | DEVICES AND METHODS FOR SEPARATING NANOPARTICLES - A device and related method for separating nanometer particles is disclosed and described. The device can include a microfluidic system including a sample input port, a fluid flow channel, and a sample output port, in which the fluid flow channel is defined by a pair of electrode walls and an insulator. A voltage device is electrically coupled to the electrode walls. The voltage device is comprised of a diode or a resistor configured to provide an electrical field within the fluid flow channel suitable for separation of nanoparticles from one another by causing a net effect of moving particles toward one of the electrode walls. | 03-19-2015 |
| 20140014513 | SYSTEMS AND METHODS FOR CHARACTERIZING A MOLECULE - Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore. | 01-16-2014 |
| 20130233709 | DUAL-PORE DEVICE - Provided is a device comprising an upper chamber, a middle chamber and a lower chamber, wherein the upper chamber is in communication with the middle chamber through a first pore, and the middle chamber is in communication with the lower chamber through a second pore, wherein the first pore and second pore are about 1 nm to about 100 nm in diameter, and are about 10 nm to about 1000 nm apart from each other, and wherein each of the chambers comprises an electrode for connecting to a power supply. Methods of using the device are also provided, in particular for sequencing a polynucleotide. | 09-12-2013 |
| 20100133105 | Optoelectronic Separation of Biomolecules - The present teachings relate to systems and methods for separation of substances such as cells, nucleic acids, and carbon nanotubes. The substances are combined with a separation medium in a liquid sample cavity, for example a microchannel, and transit through the separation by optically activated dielectrophoretic forces. The substances are advantageously labeled and visualized using a microscope and camera. | 06-03-2010 |
| 20110089033 | METHOD OF PREPARING SUGAR CHAIN SAMPLE, SUGAR CHAIN SAMPLE, AND METHOD OF ANALYZING SUGAR CHAIN - A method of preparing a sugar chain sample, for reducing unreacted labeling reagent in a sample solution containing a labeled sugar chain, includes (process 1) a process of bringing the sample solution containing the labeled sugar chain into contact with monolithic silica, so as to allow the monolithic silica to adsorb a sugar chain component; (process 2) a process of washing the monolithic silica with a washing liquid; and (process 3) a process of bringing the monolithic silica into contact with an eluant, so as to elute the adsorbed sugar chain. | 04-21-2011 |
| 20130105315 | HIGH SPEED, HIGH RESOLUTION COMPOSITIONS, METHODS AND KITS FOR CAPILLARY ELECTROPHORESIS | 05-02-2013 |
| 20110272281 | MICROFLUIDIC DEVICE FOR SEPARATING, FRACTIONATING, OR PRECONCENTRATING ANALYTES CONTAINED IN AN ELECTROLYTE - A microfluidic device for separating, fractionating, or preconcentrating analytes contained in an electrolyte having at least two reservoirs separated by at least one microchannel and/or nanochannel. At least part of the wall of the microchannel is made of and/or coated interiorly with a conducting and polarizable material or group of materials constituting a polarizable interface or a network of polarizable interfaces. In that at least one electrode or at least one electrode network is connected at least one point of the polarizable material or group of materials, the surface electrical conductance of said material being equal to at least 100 nS. | 11-10-2011 |
| 20150129427 | ELECTROEXTRACTION - The present invention relates to a process for the extraction of analyte compounds from a sample comprising one or more analytes in a donor phase into an acceptor phase, comprising the steps of: a) providing an electrically conductive donor phase comprising the compounds in a first electrically conductive solvent or solvent blend, and an electrode arranged in electrically conductive contact with the donor phase, b) providing an electrically conductive acceptor phase in electrically conductive contact with a second electrode; and c) providing an insulator phase in fluid communication with at least one of the donor phase and the acceptor phase, wherein the insulator phase is immiscible with the donor phase and/or the acceptor phase, and d) (d) applying an electrical field between the first and the second electrode. | 05-14-2015 |
| 20140021048 | Method and Apparatus for Detecting Nucleotides - A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer, such as a base of a DNA or RNA strand. The current has characteristics representative of the component of the polymer, such as characteristics representative of the detected base of the DNA or RNA strand. | 01-23-2014 |
| 20140021047 | METHOD FOR ANALYZING BIOMOLECULES USING ASYMMETRIC ELECTROLYTE CONCENTRATION - A method and system for analyzing biomolecules using a high concentration electrolytic solution and a low concentration electrolytic solution. | 01-23-2014 |
| 20130105316 | Substrate Modification Method | 05-02-2013 |
| 20120043207 | MEASUREMENT METHOD UTILIZING INTERNAL STANDARD SUBSTANCE - A subject of the present invention is to provide a measurement method using an internal standard substance in an electrophoresis where an analyte is a protein or a compound. The present invention relates to a measurement method for an analyte by an electrophoresis, characterized in that a peak of the analyte is identified by using as an internal standard substance (1) a combination of a compound I having 3 or more anion groups in a molecule and a compound II where 1 to 3 groups of the anion groups of said compound I have been substituted by cation groups, or (2) a combination of a compound III having 3 or more cation groups in a molecule and a compound IV where 1 to 3 groups of the cation groups of said compound III have been substituted by cation groups. | 02-23-2012 |
| 20120138462 | ELECTROWETTING DISPENSING DEVICES AND RELATED METHODS - A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting. | 06-07-2012 |
| 20160053313 | DIFFERENTIATION OF MACROMOLECULES AND ANALYSIS OF THEIR INTERNAL CONTENT IN SOLID-STATE NANOPORE DEVICES - Provided are solid-state nanopore platforms for fast, electronic, label-free and high-resolution analysis of biomolecules. | 02-25-2016 |
| 20140202858 | Sample Analysis Method and Solution to be Used Therein - Provided are a sample analysis method using capillary electrophoresis capable of enhancing analysis accuracy, a solution for capillary electrophoresis, and a sample analysis kit. The sample analysis method includes separating and/or detecting a substance to be analyzed in a sample through capillary electrophoresis, in which the substance to be analyzed is separated and/or detected in the presence of a pH buffer substance and a non-surfactant-type zwitterionic substance. Further, the solution for capillary electrophoresis contains a pH buffer substance, a non-surfactant-type zwitterionic substance, and water. | 07-24-2014 |
| 20120055793 | Emulsions of Ionic Liquids - The present teachings provide emulsions using ionic liquids for separation of biomolecules and related methods, compositions, and devices. | 03-08-2012 |
| 20130134040 | DISPOSABLE CARTRIDGE FOR MICROFLUIDICS SYSTEM - A disposable cartridge had body, bottom layer with first hydrophobic surface, top layer with a second hydrophobic surface, and gap between. The bottom layer is a flexible film sealingly attached at its circumference to the top layer. No spacer is between the layers. The top layer has loading sites for transferring processing liquids into the gap. In use, the bottom layer is a working film for manipulating samples in liquid droplets and is placed on an electrode array of a digital microfluidics system having base unit with cartridge accommodation site and electrode array at the site. The electrode array is supported by a bottom substrate that extends in a first plane and has individual electrodes. The system also includes a central control unit for controlling selection of the electrodes and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the gap by electrowetting. | 05-30-2013 |
| 20130153420 | Microfluidic Detection of Analytes - An apparatus and methods for concentrating samples for application to microfluidic devices are disclosed. The methods involve electrophoresing charged molecules from a high volume sample into a smaller volume. The analyte of interest can be a charged molecule or can be modified to be charged using, for example, one or more ionic moieties. | 06-20-2013 |
| 20130153421 | GRAFT COPOLYMERS, THEIR PREPARATION AND USE IN CAPILLARY ELECTROPHORESIS - The invention relates to graft copolymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; also to ultra-high molecular weight poly(N,N-dimethylacrylamide) (“poly(DMA)”) polymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; and more particularly to supports, such as capillaries, containing these polymers and methods for separating biomolecules, especially polynucleotides, using capillary electrophoresis. The graft copolymers can be prepared by, e.g., grafting polyacrylamide units onto a poly(DMA) backbone. Separation media comprising such graft copolymers or ultra-high molecular weight poly(DMA) polymers yield superior performance in the analysis and separation of biomolecules by capillary electrophoresis. | 06-20-2013 |
| 20120037507 | METHOD AND MARKER FOR DIAGNOSIS OF TUBULAR KIDNEY DAMAGE AND ILLNESSES - A method for the diagnosis of tubular kidney diseases comprising the step of determining the presence or absence or amplitude of at least three polypeptide markers in a urine sample, the polypeptide marker being selected from the markers characterized in Table 1 by values for the molecular masses and migration times. | 02-16-2012 |
| 20130146457 | ANALYTE SEQUENCING WITH NANOPORES - Provided herein are methods and systems pertaining to sequencing units of analytes using nanopores. In general, arresting constructs are used to modify an analyte such that the modified analyte pauses in the opening of a nanopore. During such a pause, an ion current level is obtained that corresponds to a unit of the analyte. After altering the modified analyte such that the modified analyte advances through the opening, another arresting construct again pauses the analyte, allowing for a second ion current level to be obtained that represents a second unit of the analyte. This process may be repeated until each unit of the analyte is sequenced. Systems for performing such methods are also disclosed. | 06-13-2013 |
| 20130146456 | ARTIFICIAL MYCOLIC ACID MEMBRANES - Provided herein are artificial membranes of mycolic acids. The membranes may be unsupported or tethered. These membranes are long lived and highly resistant to electroporation, demonstrating their general strength. The mycolic acid membranes are suitable for controlled studies of the mycobacterial outer membrane and can be used in other experiments, such as nanopore analyte translocation experiments. | 06-13-2013 |
| 20150360237 | PUNCTUATED MICROGRADIENTS FOR IMPROVED SEPARATIONS OF MOLECULES AND PARTICLES - The present invention provides devices and methods to separate and concentrate target species. In some embodiments, a punctuated continuous microchannel or parallel processing (array-based) separations are provided, the microchannel having a plurality of sequential, constrictive insulating features to form a plurality of reservoirs including a first, second and third reservoir, and a plurality of constricted passageways including a first constricted passageway that connects the first reservoir to the second reservoir and a second constricted passageway that connects the second reservoir to the third reservoir. A voltage is applied to the microchannel to create different electrical fields and/or different dielectrophoresis (DEP) gradients at each of the plurality of constricted passageways in order to separate species that have differing ratios of electrokinetic mobility to dielectrophoretic mobility. | 12-17-2015 |
| 20100096266 | METHOD AND APPARATUS FOR REAL-TIME FEEDBACK CONTROL OF ELECTRICAL MANIPULATION OF DROPLETS ON CHIP - A device for generating droplets includes a substrate comprising a reservoir site configured to hold a liquid and including a first electrode, a droplet creation site including a second electrode, and droplet separation site disposed between the reservoir site and the droplet creation site and containing an electrode. The device includes control circuitry operatively coupled to the first, second, and third electrodes. The control circuitry is configured to measure the fluid volume on the electrodes and independently adjust an applied voltage to increase/decrease the quantity of fluid. The device can move fluid onto the creation site or back onto to the reservoir site. When the fluid volume is at the desired value or range, a driving voltage is delivered to the first and second electrodes to form a new droplet. The device may generate droplets having a uniform or user-defined size smaller than the electrode. | 04-22-2010 |
| 20120061240 | ELECTROTAXIS METHODS AND DEVICES - A method of controlling nematode response in a microfluidic environment is provided comprising exposing the nematode to an electric field that induces a nematode response. In one embodiment, a method of sorting a group of nematodes based on a selected parameter is provided comprising the step of exposing the nematodes to an electric field that induces a differential response among the nematodes based on the selected parameter, wherein the differential response functions to separate the nematodes based on the selected parameter. Devices useful to achieve these methods are also provided. | 03-15-2012 |
| 20130062205 | ACTIVE MATRIX DEVICE FOR FLUID CONTROL BY ELECTRO-WETTING AND DIELECTROPHORESIS AND METHOD OF DRIVING - A microfluidic device includes a plurality of array elements configured to manipulate one or more droplets of fluid on an array, each of the array elements including a top substrate electrode and a drive electrode between which the one or more droplets may be positioned, the top substrate electrode being formed on a top substrate, and the drive electrode being formed on a lower substrate; and active matrix drive circuitry arranged to provide drive signals to the top substrate and drive electrodes of the plurality of array elements to manipulate the one or more droplets among the plurality of array elements. With respect to one or more of the array elements the active matrix drive circuitry is configured to provide the drive signals to the top substrate and drive electrodes to selectively manipulate the one or more droplets within the array element both by Electro-wetting-on-Dielectric (EWOD) and by Dielectrophoresis (DEP). | 03-14-2013 |
| 20120097539 | NANOPORE-BASED NANOPARTICLE TRANSLOCATION DEVICES - A nanoparticle translocation device includes a first reservoir having a first reservoir electrode, a second reservoir having a second reservoir electrode, and at least one nanopore providing fluid communication between the first and second reservoirs. The device also includes one or more inner electrode portions on an inner wall of the nanopore and one or more outer electrode portions disposed on an outer wall of the nanopore. The device further includes at least one DC voltage supply for selectively applying a DC voltage to each of the first reservoir electrode, the second reservoir electrode, and the outer electrode layer, where the inner electrode portions, the outer electrode portions, and the nanopore are in a substantially coaxial arrangement. | 04-26-2012 |
| 20120080313 | ELECTROOSMOTIC DEVICES - Electroosmotic (EO) devices are provided which are not subject to mechanical wear and tear and with no moving parts, and having improved flow rates and electrical properties. Atomic layer deposition can be used to prepare three electrical terminal active zeta potential modulated EO devices from porous membranes. First, second, and further thin layers of materials can be formed with the pores. Thus, embedded electrodes can be formed along the length of the pores. The zeta potential in the pores can be modified by use of a voltage potential applied the embedded electrode, thereby achieving active control of surface zeta potential within the pores and active control of flow through the pores. | 04-05-2012 |
| 20140034496 | CAPILLARY TUBES FOR ELECTROPHORESIS - The present invention relates to a plastic capillary tube for capillary electrophoresis, in which the plastic capillary tube has an inlet opening and an outlet opening and, furthermore, has at least one hole in the capillary tube wall and the diameter of the hole on the inside of the capillary tube wall d | 02-06-2014 |
| 20160103097 | HIGH SPEED, HIGH RESOLUTION COMPOSITIONS, METHODS AND KITS FOR CAPILLARY ELECTROPHORESIS - The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided. | 04-14-2016 |
| 20140102901 | NEGATIVE DIELECTROPHORESIS FOR SELECTIVE ELUTION OF IMMUMO-BOUND PARTICLES - The procedure of dielectric electrophoresis (dielectrophoresis or DEP) utilizes field-polarized particles that move under the application of positive (attractive) and/or negative (repulsive) applied forces. This invention uses negative dielectric electrophoresis (negative dielectrophoresis or nDEP) within a microchannel separation apparatus to make particles move (detached) or remain stationary (attached). In an embodiment of the present invention, the nDEP force generated was strong enough to detach Ag-Ab (antigen-antibody) bonds, which are in the order of 400 pN (piconewtons) while maintaining the integrity of the system components. | 04-17-2014 |
| 20140102899 | PLASMA TREATMENT FOR DNA BINDING - The invention provides a composition including DNA bonded to a plasma-treated surface, the plasma can be any suitable plasma, such as an argon plasma, a compressed air plasma, a flame-based plasma or a vacuum plasma. Surfaces treatable by the methods of the invention include ceramic, metal, fabric and organic polymer surfaces. The DNA can be any DNA, such as a marker DNA, which can be linear or circular, single-stranded or double stranded and from about 25 bases to about 10,000 bases in length. Also provided is a method of binding DNA to a surface, including the steps of exposing the surface to a plasma to produce a plasma-treated surface; and applying DNA to the plasma-treated surface to produce surface bound DNA on the treated surface. A system for binding DNA to a surface is also disclosed, the system includes a plasma generator adapted to treating a surface with a plasma to produce a plasma-treated surface; and an applicator containing DNA adapted to applying DNA to the plasma-treated surface to produce surface bound DNA on the plasma-treated surface. | 04-17-2014 |
| 20140102900 | Microfluidic Devices And Applications Thereof - In one aspect, single-sided microfluidic devices are described herein. In some embodiments, a single-sided microfluidic device comprises a substrate, a photoconductive layer positioned over the substrate, electrical contacts in electrical communication with the photoconductive layer, and a dielectric assembly positioned over the photoconductive layer. The dielectric assembly comprises a hydrophobic surface for receiving a liquid. In some embodiments, the dielectric assembly has an effective capacitance of about 10 μF/m | 04-17-2014 |
| 20110297542 | METHOD OF FABRICATION OF MONOLITHIC STATIONARY PHASES FOR SEPARATIONS, AND METHODS OF SEPARATION USING SUCH STATIONARY PHASES - Analysis tools for use in analytical separations are provided including a stationary phase comprising an electroconducting material, for example, a conducting polymer. There is further provided a method for manufacturing monolithic stationary phases in various formats including in chip-format, column or capillary column format and method of separation using stationary phases. Also provided is a method for analytical separation comprising selective manipulation of the stationary phase making it is possible to tune the device to specific applications. | 12-08-2011 |
| 20150090591 | APPARATUS, SYSTEMS, AND METHODS FOR CAPILLARY ELECTROPHORESIS - An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided. | 04-02-2015 |
| 20110297543 | Autosomal-Dominant Polycystic Kidney Disease (ADPKD) - The process for the diagnosis, early detection and prognosis of the clinical development of autosomal-dominant polycystic kidney disease (ADPKD) comprises the step of determining the presence or absence or amplitude of at least three polypeptide markers in a urine sample, the polypeptide markers being selected from the markers characterized in Table 1 by values for the molecular masses and migration times. | 12-08-2011 |
| 20140332381 | FASTER RESISTIVE-PULSE SENSING TOGETHER WITH PHYSICAL AND MECHANICAL CHARACTERIZATION OF PARTICLES AND CELLS - A method for multiplex characterization of individual particles by their size, shape, mechanical properties (deformability), and chemical affinity to recognition agents. The analysis can be performed from concentrated solutions. The method detects transient sticking of particles in the pore and points to its location along a pore axis. If a pore is decorated with a recognition agent for an analyte present in a solution, it is possible to distinguish specific binding at the place of the recognition agent, and non-specific adsorption of the analyte. The method confirms whether any individual particle or hydrogel completely translocates the pore and allows unambiguous detection and characterization of multiple particles or hydrogels in the pore, which would previously corrupt the results, so that higher analyte concentrations can be used for faster analysis. High aspect ratio of the pores (ratio of pore length and diameter) together with the pattern of ion current pulses also allow passage of the same particle or cell multiple times without letting the cell exit the pore. | 11-13-2014 |
| 20130270114 | Digital MicroFluidics System with Swappable PCB's - Digital microfluidics system manipulates samples in liquid droplets within disposable cartridges that have bottom layer, top layer, and gap between the bottom and top layers. The system has a base unit with cartridge accommodation sites and a central control unit for controlling selection of individual electrodes of electrode arrays and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the cartridges by electrowetting. The system further has board accommodation sites located at the cartridge accommodation sites that each can take up a swappable electrode board having an electrode array and electrical board contact elements individually connected to electrodes of the electrode array. Each board accommodation site has electrical base unit contact elements that are connected to the central control unit and that are configured to engage with the electrical board contact elements of a swappable electrode board that is placed at the board accommodation site. | 10-17-2013 |
| 20140360876 | METHOD AND APPARATUS FOR CONTROLLING MATERIALS THROUGH A THROUGH-HOLE - A system that incorporates the subject disclosure may include, for example, a method for selectively applying an electrical potential to a top surface of a membrane having a nanopore to repel or attract a molecular strand from the top surface of the membrane, applying a second electrical potential to a bottom surface of the membrane to repel or attract the molecular strand from the bottom surface of the membrane, applying a third electrical potential to an electrolyte solution to apply a transport force on the molecular strand to displace a section of the molecular strand into the nanopore, arresting the section of the molecular strand in the nanopore by adjusting of the first electrical potential, the second electrical potential, the third electrical potential, or combinations thereof, and measuring a signal at the nanopore to identify the section of the molecular strand. Other embodiments are disclosed. | 12-11-2014 |
| 20110192723 | Systems and methods for manipulating a molecule in a nanopore - Techniques for manipulating a molecule in a nanopore embedded in a lipid bilayer are described. In one example, an acquiring electrical stimulus level is applied across a lipid bilayer wherein a region of the lipid bilayer containing the nanopore is characterized by a resistance and wherein the acquiring electrical stimulus level tends to draw the molecule from a surrounding fluid into the nanopore, a change in the resistance of the lipid bilayer resulting from the acquisition of at least a portion of a molecule into the nanopore is detected, the acquiring electrical stimulus level is changed to a holding electrical stimulus level wherein the portion of the molecule remains in the nanopore upon the changing of the acquiring electrical stimulus level to the holding electrical stimulus level. | 08-11-2011 |
| 20110192724 | HIGH RESOLUTION FOCUSING AND SEPARATION OF PROTEINS IN NANOFLUIDIC CHANNELS - Exemplary embodiments provide systems and methods for concentrating, focusing and/or separating proteins using nanofluidic channels and/or their arrays. In embodiments, low-abundance proteins can be focused and separated with high resolution using separation techniques including isoelectric focusing (IEF), and/or dynamic field gradient focusing (DFGF) in combination with nanofluidic channels and/or multi-gate nanofluidic field-effect-transistors (FETs). | 08-11-2011 |
| 20150300984 | ELECTRO-FLUIDIC FLOW PROBE - An apparatus for an electro-fluidic flow probe includes a body portion including an electro-fluidic bias tee for receiving (i) a fluid electrolyte and (ii) an electrical connection for providing an electrical potential to the fluid electrolyte; a first inlet including a tube extending from the first inlet to an outlet through the electro-fluidic bias tee; and a second inlet including the electrical connection having a wire that extends from the second inlet to the outlet through the electro-fluidic bias tee to transfer the electrical potential to a device under test. | 10-22-2015 |
| 20120193231 | DNA SEQUENCING USING MULTIPLE METAL LAYER STRUCTURE WITH ORGANIC COATINGS FORMING TRANSIENT BONDING TO DNA BASES - A nanodevice is provided. A reservoir is filled with an ionic fluid. A membrane separates the reservoir, and the membrane includes electrode layers separated by insulating layers in which the electrode layers have an organic coating. A nanopore is formed through the membrane, and the organic coating on the electrode layers forms transient bonds to a base of a molecule in the nanopore. When a first voltage is applied to the electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels through the transient bonds formed to the base to be measured as a current signature for distinguishing the base. | 08-02-2012 |
| 20150300986 | FORMATION OF LAYERS OF AMPHIPHILIC MOLECULES - To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution. | 10-22-2015 |
| 20150300985 | FLUID HANDLING DEVICE AND FLUID HANDLING METHOD - This fluid handling device (micro fluid chip ( | 10-22-2015 |
| 20110139621 | MICROFLUIDIC CELL - A microfluidic cell for the dielectrophoretic separation, accumulation, and/or lysis of polarizable bioparticles, including an interdigital electrode system composed of two electrode groups having interdigitated electrodes, a flat electrode in which the interdigital electrode system and the flat electrode are situated on opposite sides of the cell in order to improve the separation, accumulation and/or lysis characteristics. Moreover, a microfluidic system which includes such a microfluidic cell, and use thereof, and a method for separating, accumulating, and/or lysing polarizable bioparticles is also described. | 06-16-2011 |
| 20120199480 | ELECTROPHORETIC ANALYSIS METHOD - Provided is a means for accurately analyzing a protease by electrophoresis. | 08-09-2012 |
| 20150136603 | WATER-SOLUBLE, UV-ABSORBING AND/OR FLOURESCENT COMPONENTS WITH VERY HIGH ISOELECTRIC POINTS - Water-soluble, UV-absorbing and/or fluorescing compound having an isoelectric point greater than 10, can be obtained using molecules chosen from the group of molecules consisting of those containing at least one secondary alcohol OH group with a pK | 05-21-2015 |
| 20150136602 | UNIVERSAL SAMPLE PREPARATION SYSTEM AND USE IN AN INTEGRATED ANALYSIS SYSTEM - The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft | 05-21-2015 |
| 20150136601 | Nanochannel Arrays and Their Preparation and Use for High Throughput Macromolecular Analysis - Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods. | 05-21-2015 |
| 20110214990 | KIDNEY CELL CARCINOMA - The process for the diagnosis of a renal cell carcinoma comprises the step of determining the presence or absence or amplitude of at least three polypeptide markers in a urine sample, wherein said polypeptide markers are selected from the markers as characterized in Table 1 by molecular masses and migration times. | 09-08-2011 |
| 20110132761 | METHODS AND DEVICES FOR ANALYTE DETECTION - Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays. | 06-09-2011 |
| 20120186977 | DEVICES AND METHODS FOR ELECTROOSMOTIC TRANSPORT OF NON-POLAR SOLVENTS - A microdevice for supporting a flowing nonpolar fluid is disclosed. The microdevice includes a substrate that is at least partially coated by one or more amphiphilic layers. Methods for using the device in biological and chemical assays are also disclosed. | 07-26-2012 |
| 20130213809 | MEASURING SYSTEM AND METHOD FOR AUTONOMOUS MEASUREMENT OF AN ION CONCENTRATION WITH MICROCHIP CAPILLARY ELECTROPHORESIS - A measuring system and method are disclosed for measuring an ion concentration in a process liquid using capillary electrophoresis. In at least one embodiment, the measuring system includes an inlet connectable to a process for supplying the process liquid; a throughflow system connected to the inlet; a capillary electrophoresis measuring device connected to the throughflow system and provided with a detector and measuring capillary; and a data processing unit connected operatively during use to the capillary electrophoresis measuring device. | 08-22-2013 |
| 20150144489 | DISPOSABLE CARTRIDGE FOR MICROFLUIDICS SYSTEMS - A disposable cartridge used in a digital microfluidics system has a bottom layer with first hydrophobic surface, a rigid cover plate with second hydrophobic surface, and a gap there-between. The bottom layer is a flexible film on an uppermost surface of a cartridge accommodation site of a system, attracted to and spread over the uppermost surface by an underpressure. A lower surface of the plate and the flexible bottom layer are sealed to each other. The assembled cartridge is removed from the cartridge accommodation site in one piece and potentially includes samples and processing fluids. The system has a base unit and a cartridge accommodation site with an electrode array of individual electrodes and a central control unit for controlling selection of individual electrodes and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the gap by electrowetting. | 05-28-2015 |
| 20150316505 | Methods And Devices For Sequencing Nucleic Acids In Smaller Batches - The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency. | 11-05-2015 |
| 20140090979 | Paired Laser and Electrokinetic Separation, Manipulation, and Analysis Device - The combined value of integrating optical forces and electrokinetics allows for the pooled separation vectors of each to be applied, providing for separation based on combinations of features such as size, shape, refractive index, charge, charge distribution, charge mobility, permittivity, and deformability. The interplay of these separation vectors allow for the selective manipulation of analytes with a finer degree of variation. Embodiments include methods of method of separating particles in a microfluidic channel using a device comprising a microfluidic channel, a source of laser light focused by an optic into the microfluidic channel, and a source of electrical field operationally connected to the microfluidic channel via electrodes so that the laser light and the electrical field to act jointly on the particles in the microfluidic channel. Other devices and methods are disclosed. | 04-03-2014 |
| 20140131203 | INTEGRATED NANOPORE AND PAUL TRAP MECHANISM FOR DNA CAPTURE AND MOTION CONTROL - A mechanism is provided for capturing a molecule via an integrated system. An alternating voltage is applied to a Paul trap device in an electrically conductive solution to generate electric fields. The Paul trap device is integrated with a nanopore device to form the integrated system. Forces from the electric fields of the Paul trap device position the molecule to a nanopore in the nanopore device. A first voltage is applied to the nanopore device to capture the molecule in the nanopore of the nanopore device. | 05-15-2014 |
| 20140131202 | INTEGRATED NANOPORE AND PAUL TRAP MECHANISM FOR DNA CAPTURE AND MOTION CONTROL - A mechanism is provided for capturing a molecule via an integrated system. An alternating voltage is applied to a Paul trap device in an electrically conductive solution to generate electric fields. The Paul trap device is integrated with a nanopore device to form the integrated system. Forces from the electric fields of the Paul trap device position the molecule to a nanopore in the nanopore device. A first voltage is applied to the nanopore device to capture the molecule in the nanopore of the nanopore device. | 05-15-2014 |
| 20120241321 | POLYPEPTIDE MARKER FOR DIAGNOSING AND ASSESSING VASCULAR DISEASES - The invention relates to a method for diagnosing vascular diseases, comprising the step in which the presence, absence or amplitude of at least three polypeptide markers is determined in a urine sample, wherein the polypeptide markers are selected from the markers characterized in table 1 by values for the molecular masses and the migration time. | 09-27-2012 |
| 20140116881 | Pens For Biological Micro-Objects - Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens. | 05-01-2014 |
| 20160011150 | Buffer Composition | 01-14-2016 |
| 20140151227 | FIELD EFFECT BASED NANOSENSOR FOR BIOPOLYMER MANIPULATION AND DETECTION - A mechanism is provided for manipulating a molecule. The molecule is driven into a nanochannel filed with electrically conductive fluid. A first vertical electric field is created inside the nanochannel to slow down the molecule and/or immobilize the molecule. The molecule is stretched into non-folded linear chains by the first vertical electric field and a horizontal electric field. Monomers of the molecule are sequentially read. | 06-05-2014 |
| 20140151228 | FIELD EFFECT BASED NANOSENSOR FOR BIOPOLYMER MANIPULATION AND DETECTION - A mechanism is provided for manipulating a molecule. The molecule is driven into a nanochannel filed with electrically conductive fluid. A first vertical electric field is created inside the nanochannel to slow down the molecule and/or immobilize the molecule. The molecule is stretched into non-folded linear chains by the first vertical electric field and a horizontal electric field. Monomers of the molecule are sequentially read. | 06-05-2014 |
| 20140116882 | Microfluidic dielectrophoresis system - A microfluidic dielectrophoresis system includes: one supply device for a liquid medium having particles contained therein, N≧2 microfluidic, dielectrophoretically active channels, which are equipped with electrodes, lines for the fluidic connection of the supply device to the channels, for the connection of the channels to one another, and for the drainage of the medium and/or the particles from the channels, and valves for setting the flow direction of the medium in the lines, the dielectrophoretically active channels being situated and being connected by lines in such a way that they may be operated connected in parallel and in series by switching the valves in relation to the flow direction of the medium and the electrodes of the various channels are activatable independently of one another. | 05-01-2014 |
| 20140174926 | MOLECULAR DIAGNOSTICS PLATFORM - A method of mixing a droplet, the method comprising providing a droplet on a surface, forming the droplet into a first “U” shape having a bottom region and two terminal ends, and simultaneously merging the terminal ends and splitting the droplet at the bottom region to form a second “U” shape which is substantially opposite the first “U” shape. | 06-26-2014 |
| 20140008224 | METHOD AND MICROSYSTEM FOR DETECTING ANALYTES WHICH ARE PRESENT IN DROPS OF LIQUID - A detection method of detecting analytes of interest which are present in a liquid. The detection method including the steps of forming drops of liquid on a first surface by capillary breaking of a finger of liquid, which is initially formed by liquid dielectrophoresis. The thus formed drops each come into contact with a different detection surface, which is arranged facing the first surface. Analytes of interest which are present in each of the drops are detected at the corresponding detection surface. | 01-09-2014 |
| 20140034497 | CHIP SET-UP AND HIGH-ACCURACY NUCLEIC ACID SEQUENCING - The present disclosure provides devices, systems and methods for sequencing nucleic acid molecules. Nucleic acid molecules can be sequenced with a high accuracy (e.g., greater than 97% in a single pass) using a chip comprising an array of independently addressable nanopore sensors at a density of at least about 500 sites per 1 mm | 02-06-2014 |
| 20140034498 | Localized Chemical Microgradients - A device for creating microgradients in solution is disclosed. The device contains a microfluidic channel with openings at each end and two or more small apertures to a bath. Electrodes are placed in the openings at either end of the channel and an electrical power supply is connected to the electrodes. Several distinct current paths exist from one end of the channel to the other. For example current may flow from one electrode, through a portion of the channel, through an aperture into the bath, back through another aperture into the channel, and along another portion of the channel to the other electrode. Current flows along all possible connected paths when an electric field is applied along the channel and induces fluid flow into and out of the apertures in the channel. Fluid flow through the apertures results in the formation of microgradients in solution near the microfluidic channel device. | 02-06-2014 |
| 20140048416 | SYSTEMS AND METHODS FOR SINGLE-MOLECULE DETECTION USING NANOPORES - A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any. | 02-20-2014 |
| 20120181175 | FILLER FOR ANALYZING CAPILLARY ELECTROPHORESIS-BASED SINGLE STRAND CONFORMATION POLYMORPHISM, AND METHOD FOR USING THE FILLER FOR ANALYZING CAPILLARY ELECTROPHORESIS-BASED SINGLE STRAND CONFORMATION POLYMORPHISM - The present invention relates to filler for analyzing capillary electrophoresis-based single strand conformation polymorphism, and to a method for using the filler for analyzing capillary electrophoresis-based single strand conformation polymorphism, and more particularly, to filler for analyzing capillary electrophoresis-based single strand conformation polymorphism, the filler containing a polymer micelle formed by dispersing a sandwich-block copolymer comprising (Hydrophilic group)-(Hydrophobic group)-(Hydrophilic group) in an aqueous medium, and to a method for using the filler for analyzing capillary electrophoresis-based single strand conformation polymorphism. | 07-19-2012 |
| 20160069835 | ANALYSIS METHOD AND ANALYSIS SYSTEM - A method for analyzing a sample using capillary electrophoresis is provided. By the method, following steps are performed. First, an original sample and an anionic group-containing compound are fixed to form a mixed sample, where the original sample contains an analysis component to be analyzed and a sub component other than the analysis component. Then, an aggregate of the sub component and the anionic group-containing compound is removed from the mixed sample. Then, electrophoresis is performed in a capillary tube with respect to a complex in which the analysis component and the anionic group-containing compound are bound to each other, while the mixed sample is continuously supplied. | 03-10-2016 |
| 20110139620 | MICROFLUIDIC CELL - A microfluidic cell for the dielectrophoretic separation, accumulation, and/or lysis of polarizable bioparticles, including an interdigital electrode system composed of two electrode groups having interdigitated electrodes, and a micromixer having microchannels and microelevations. The interdigital electrode system and the micromixer are situated on the same side of the cell to improve the separation, accumulation, and/or lysis characteristics. Moreover, also described is a microfluidic system which includes such a microfluidic cell, and use thereof, and a method for separating, accumulating, and/or lysing polarizable bioparticles. | 06-16-2011 |
| 20110139622 | METHOD OF DETECTING ANALYTE-BIOMOLECULE INTERACTIONS - The invention provides methods for detecting an interaction between an analyte and a biomolecule. The method comprises separating at least one biomolecule according to its isoelectric point in the presence of a given analyte and detecting an interaction between the analyte and a biomolecule using fluorescence anisotropy. The method may further comprise collecting the analyte-biomolecule complex and analyzing the biomolecule. | 06-16-2011 |
| 20110186432 | BUFFERS FOR ELECTROPHORESIS AND USE THEREOF - Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described. | 08-04-2011 |
| 20110259742 | Droplet Based Miniaturized Device With On-Demand Droplet-Trapping, -Fusion, And -Releasing - The present invention refers to a droplet-based miniaturized device with on-demand droplet-trapping, -fusion, and -releasing. The device makes use of different electrical fields for directing droplets into microwells and releasing them from the same. In another aspect, the present invention refers to a system comprising such a microfluidic device and a method of operating it. | 10-27-2011 |
| 20110209999 | Capillary driven lateral flow devices - A lateral flow device includes a porous medium layer having a two-dimensional shape in plan view that is capable of supporting near-constant velocity capillary-driven fluid flow and can be combined with electrodes in a manner to achieve to achieve electrokinetic molecule separation. | 09-01-2011 |
| 20110226621 | TARGET RECOGNITION MOLECULE AND A METHOD FOR IMMOBILIZING THE SAME - There is provided a novel target recognition molecule. The target recognition molecule has a specific reactivity, and can be densely self-assembled and immobilized reversibly or irreversibly at a predetermined site in a microfluidic device. And The target recognition molecule including: (1) a target recognition peptide segment having an amino acid sequence which specifically interacts with a target substance capable of causing an immune reaction; and (2) an electrostatically-charged segment which is provided with three or more electrostatically-charged functional groups capable of being electrically charged to the same polarity in the same solution. | 09-22-2011 |
| 20150114837 | Capillary Electrophoresis for Reservoir Fluid Analysis at Wellsite and Laboratory - A method improves the capability for testing a fluid sample, e.g. testing a reservoir sample of hydrocarbon fluid. The methodology comprises positioning a capillary electrophoresis system within an enclosed chamber system. The enclosed chamber system preserves the desired downhole reservoir conditions during testing of the reservoir sample. In some applications, the reservoir sample is divided into a plurality of capillaries of the capillary electrophoresis system to enable testing of the reservoir sample with different types of detectors in one capillary electrophoresis system. The method can also be applied to depressurized reservoir samples. | 04-30-2015 |
| 20110155574 | Molecular Characterization with Molecular Speed Control - Provided is a first reservoir for containing a liquid solution including a molecule to be characterized and a second reservoir for containing a liquid solution. A solid state support includes a nanopore having a molecular inlet providing a fluidic connection to the first reservoir and a molecular outlet providing a fluidic connection to the second reservoir. An electrical connection is disposed between the first and second reservoirs to apply a molecular translocation voltage across the nanopore between the molecular inlet entrance and outlet exit. At least one electrical probe is disposed at the nanopore to apply a first voltage bias with respect to translocation voltage to slow progression of a molecule through the nanopore between the molecular inlet and outlet and to apply a second voltage bias with respect to translocation voltage to cause the molecule to proceed through the nanopore between the molecular inlet and outlet. | 06-30-2011 |
| 20110155573 | METHOD OF ANALYZING HEMOGLOBIN BY ELECTROPHORESIS - A method for analyzing hemoglobin by electrophoresis, capable of analyzing hemoglobin A1c (HbA1c) and modified hemoglobin with improved accuracy in a shortened analysis time is provided. The method for analyzing hemoglobin by electrophoresis includes performing electrophoresis under conditions in which an acidic substance having two or more carboxyl groups is present in an electrophoresis solution. At least two of the carboxyl groups of the acidic substance each have an acid dissociation constant (pK | 06-30-2011 |
| 20110174621 | Method for Analyzing Sample by Electrophoresis and Use of the Same - A sample analysis method with improved separation accuracy is provided. The method relates to a method for analyzing a sample by electrophoresis using an electrophoresis apparatus provided with a channel and a sample reservoir formed in the channel. The method includes: placing the sample in the sample reservoir of the electrophoresis apparatus with the channel being filled with an electrophoresis running buffer; and performing electrophoresis by applying a voltage to both ends of the channel. The concentration of at least one of a) and b) is set to be approximately the same between the sample and the electrophoresis running buffer; wherein a) and b) are defined as follows:
| 07-21-2011 |
| 20110180408 | ENHANCED METHOD FOR DETECTING AND/OR QUANTIFYING AN ANALYTE IN A SAMPLE - The invention relates to an enhanced method of detecting and/or quantifying at least one analyte in a sample. | 07-28-2011 |
| 20110266151 | Microfluidic systems with electronic wettability switches - The present invention concerns microfluidic systems with printed surface structured electronically controllable wettability switches for efficient manipulation of small amounts of fluids. The high performance microfluidic systems of the invention can be used in many applications, e.g. in rapid DNA separation and sizing, cell manipulation, cell sorting and molecule detection. | 11-03-2011 |
| 20110284375 | Microfluidic device and analyzing device using the same - The conventional micropump and the conventional micromixer have the following problems. In a mechanical or hydrodynamic method, the structure of the inside of a flow path is complex so as to easily cause clogging, and manufacturing cost is high, and dead volume is large. Additionally, in an electrical method, the conventional micropump or the conventional micromixer was incapable of operating with a liquid having the concentration of a physiological saline that is important in the medical or biological field although the structure of the flow path is simple. These problems are solved by applying an AC voltage to a pair of electrodes in which an electrode-to-electrode gap between the pair of electrodes is vertically arranged and by generating the flow of a fluid in the direction opposite to gravity along the electrode-to-electrode gap. A micropump ( | 11-24-2011 |
| 20090127114 | MULTI-CHANNEL CAPILLARY ELECTROPHORESIS MICROCHIPS AND USES THEREOF - A multi-channel capillary electrophoresis microchip for analysis of multiple samples comprising: at least two sample reservoirs, at least two sample channels corresponding to said sample reservoirs, a sample buffer reservoir, a sample waste reservoir, a separation buffer reservoir, a separation waste reservoir, a sample loading channel, and a separation channel, wherein each of said sample channels is connected at one end to said sample loading channel at a place between said sample buffer reservoir and said intersection of said sample loading channel and said separation channel, and the other end of each of said sample channels is connected to each of said sample reservoirs. A method of capillary electrophoresis separation for sequentially analysis of multiple samples. | 05-21-2009 |
| 20080230389 | Electrochemical Detector Integrated on Microfabricated Capillary Electrophoresis Chip and Method of Manufacturing the Same - An electrochemical detector integrated on a capillary electrophoresis chip according to the present invention includes: a first substrate having a microchannel; a second substrate adapted to mate with the first substrate and having at least one peripheral electrode for conducting electrophoresis of a sample injected along the microchannel of the first substrate, in which a separation channel is formed along the microchannel by bonding the first substrate with the second substrate; a first electrode, made of indium tin oxide (ITO), formed on the first substrate to be positioned over the separation channel; and a second electrode, made of indium tin oxide (ITO), formed on the second substrate to be positioned under the separation channel, and spaced apart from the first electrode at a predetermined interval, wherein the first electrode and the second electrode constitute a detector to measure electrical characteristics of the sample passing along the separation channel. According to the present invention, since the specific characteristics of a sample can be evaluated by measuring the electrical or genetic characteristics of the sample flowing along the microchannel formed in a chip using a detector, a chip for a micro-analysis system having a simple structure can be realized. | 09-25-2008 |
| 20080230388 | Rapid Homogeneous Immunoassay Using Electrophoresis - The present invention concerns methods, compositions and apparatus for detection and/or determination of concentration of target molecules. In preferred embodiments the methods comprise allowing target molecules to form a complex with a first binding agent conjugated to a detection molecule and a second binding agent conjugated to a capture molecule, allowing the complex to further bind to an essentially uncharged polymer capable of binding to the capture agent, and performing a vertical gradient electrophoresis to separate the complex from unbound target, first and second binding agents and polymer. The intact complex is concentrated by electrophoresis at a stacking layer and the detection molecule is detected and/or quantified. Because the complex contains a very high mass to charge ratio, it becomes essentially immobile at the stacking layer, while unbound components migrate through the stacking layer and are separated from the complex. This provides a very rapid and sensitive assay that can detect very low concentrations of target molecules in short time. | 09-25-2008 |
| 20090188795 | Cell sorting device and method of manufacturing the same - A system, method and apparatus employing the laminar nature of fluid flows in microfluidic flow devices in separating, sorting or filtering colloidal and/or cellular particles from a suspension in a microfluidic flow device is disclosed. The microfluidic flow device provides for separating a particle within a suspension flow in a microfluidic flow chamber. The chamber includes a microfluidic channel comprising at least one inlet port for receiving a suspension flow under laminar conditions, a first outlet port and a second outlet port. The chamber further includes an interface for translating a particle within the channel. The first outlet port receives a first portion of the suspension exiting the said channel and the second outlet port receives the particle in a second portion of the suspension exiting the channel. | 07-30-2009 |
| 20080202931 | Ion Specific Control of the Transport of Fluid and Current in Fluidic Nanochannels - The present disclosure provides various means for optimizing fluid transport in micro and nanofluidic devices. Such means may be used to construct fluidic devices specifically suited to particular tasks such as molecular and biomolecular sensing and analysis, biosensors for clinical diagnostics; memory devices; screening devices for pharmaceutical applications; the provision of biologically functionalized surfaces; high throughput screening for pharmaceutical applications; controlled drug delivery; medical diagnosis; environmental monitoring; chemical and biological warfare agent sequestration; actuator development; power sources; transistors; diodes; electrochemical pumps; and bio-fuel cell development. The present disclosure further provides methods of controlling the direction of electric current and fluid flow in such devices. | 08-28-2008 |
| 20080202933 | Microfluidic Detection of Analytes - An apparatus and methods for concentrating samples for application to microfluidic devices are disclosed. The methods involve electrophoresing charged molecules from a high volume sample into a smaller volume. The analyte of interest can be a charged molecule or can be modified to be charged using, for example, one or more ionic moieties. | 08-28-2008 |
| 20080230387 | Microfluidic Devices and Methods of Using Same - A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses. | 09-25-2008 |
| 20090166200 | SERUM COMPONENTS THAT BIND TO THREAT AGENTS - Low molecular weight serum components (less than 10,000 m.w.), in vaccinated animals and a human subject who has been exposed to a threat agent inadvertently, bound to purified O-polysaccharide (OPS, a polymer of formamido-mannose) and a candidate of a threat agent, such as | 07-02-2009 |
| 20090084679 | APPARATUS AND METHOD FOR TRAPPING BEAD BASED REAGENTS WITHIN MICROFLUIDIC ANALYSIS SYSTEMS - The present invention provides an on-chip packed reactor bed design that allows for an effective exchange of packing materials such as beads at a miniaturized level. The present invention extends the function of microfluidic analysis systems to new applications including on-chip solid phase extraction (SPE) and on-chip capillary electrochromatography (CEC). The design can be further extended to include integrated packed bed immuno- or enzyme reactors. | 04-02-2009 |
| 20080257734 | Method for analyzing analytical sample in high-throughput type automatic analytical device - In a method for analyzing a sample by using an analytical device having capillary channels, a solvent for the sample is prepared. The solvent includes at least one component selected from the group consisting of polyvalent alcohols, sugars, and hydrophilic polymers. A sample solution is prepared by mixing an analytical sample with the solvent, and the sample solution is analyzed by using the analytical device. | 10-23-2008 |
| 20090045058 | MICROCHIP AND ANALYSIS METHOD USING THE MICROCHIP - With the object of realizing a microchip that allows drying and fixing of a substance that is the object of analysis in a shorter time while maintaining the separation capabilities of a separation operation, a microchip used to implement a separation operation of an analysis sample without contamination or spillage is composed of a substrate and a cover, the substrate being provided with a trench-shaped channel in its upper surface and substrate reservoirs that are linked with this channel, and the cover hermetically sealing the upper surface of the channel, attachable to or detachable from the substrate, and provided with through-holes formed at positions corresponding to the substrate reservoirs, cover reservoirs that are formed on the inner sides of the through-holes for holding liquid that is introduced when the cover is in the state of hermetically sealing the upper surface of the channel, and partitions that are formed on the bottom surfaces of the cover reservoirs. The areas of the openings of the partitions are smaller than the areas of the openings of the cover reservoirs that are over the partitions. | 02-19-2009 |
| 20090200166 | Method of Analyzing Hemoglobin by Capillary Eletrophoresis - The present invention is directed to methods of analyzing hemoglobin by capillary electrophoresis involving electrophoresing a hemoglobin-containing sample in the presence of chaotropic anion. | 08-13-2009 |
| 20090200165 | USE OF POLYMER REAGENTS TO MODULATE AND CONTROL ELECTROKINETIC FLOWS IN A MICRO- OR NANOFLUIDIC DEVICE - The invention concerns the field of microfluidics and in particular that of electrophoresis on a micro- or nanofluidic device. More particularly, the invention concerns the use of a reactive polymer coating adapted to be submitted to a phase separation under the influence of an external stimulation of chemical or physical type, to modulate the electrokinetic flows (electroosmotic or electrophoretic flows during electrophoresis for example) in a micro- or nanofluidic device. The invention also concerns a method for varying the electrokinetic flows in such a device using said coating, as well as the micro- or nanofluidic devices comprising at least one channel or one capillary tube whereof the inner surface is covered at least partly with such a coating. | 08-13-2009 |
| 20090020427 | Plastic microfluidic separation and detection platforms - Plastic electrophoresis separation chips are provided comprising a plurality of microfluidic channels and a detection window, where the detection window comprises a thin plastic; and the detection window comprises a detection region of each microfluidic channel. Such chips can be bonded to a support provided an aperture is provided in the support to allow detection of samples in the electrophoresis chip at the thin plastic detection window. Further, methods for electrophoretically separating and detecting a plurality of samples on the plastic electrophoresis separation chip are described. | 01-22-2009 |
| 20090152114 | Complex formation method and separation method - An object of the present invention is to provide a method for forming a complex between an analyte or an analogue thereof, and a substance formable the complex with said analyte or said analogue thereof (the complex forming substance), in a short time and in high reaction efficiency, and a method for separating a complex formed, and a complex forming substance not involved in formation of said complex or an analogue not involved in formation of said complex rapidly, simply and in high accuracy, along with a method for measuring an analyte in a sample in high sensitivity. | 06-18-2009 |
| 20090194419 | Method and Apparatus to Perform Limited Two Dimensional Separation of Proteins and Other Biologicals - A method and apparatus are provided for performing capillary isoelectric focusing followed by mobilization of the focused zones by induced hydrodynamic flow or chemical mobilization. These two dimensions of separation are integrated with real-time whole-channel electrophoresis detection and automatic sample injection to achieve a separation resolution superior to that obtainable using known orthogonal capillary two dimensional arrangements. | 08-06-2009 |
| 20090032399 | Methods for Forming Small-Volume Electrical Contacts and Material Manipulations With Fluid Microchannels - A microfabricated device employing a bridging membrane and methods for electrokinetic transport of a liquid phase biological or chemical material using the same are described. The bridging membrane is deployed in or adjacent to a microchannel and permits either ionic current flow or the transport of gas species, while inhibiting the bulk flow of material. The use of bridging membranes in accordance with this invention is applicable to a variety of processes, including electrokinetically induced pressure flow in a region of a microchannel that is not influenced by an electric field, sample concentration enhancement and injection, as well as improving the analysis of materials where it is desired to eliminate electrophoretic bias. Other applications of the bridging membranes according to this invention include the separation of species from a sample material, valving of fluids in a microchannel network, mixing of different materials in a microchannel, and the pumping of fluids. | 02-05-2009 |
| 20080257733 | NON-THERMOSENSITIVE MEDIUM FOR ANALYZING SPECIES IN A CHANNEL AND FOR MINIMIZING ADSORPTION AND/OR ELECTROOSOMOSIC PHENOMENA - An aqueous liquid medium for analyzing, purifying or separating species in an element having walls or for treating the walls of an element. The medium includes at least a polymer consisting of several polymeric segments. The polymer is of the irregular block-copolymer or irregular comb-like polymer type and has on the average at least three junction points between polymeric segments of different chemical or topological nature. The medium may be used in methods for analyzing, purifying or separating species and methods for treating an element to be contacted with a fluid and/or species contained in the fluid during preservation, transport, analysis, purification or separation of the fluid. | 10-23-2008 |
| 20080223721 | High Efficiency and High Precision Microfluidic Devices and Methods - New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available. | 09-18-2008 |
| 20080223720 | MICROCHANNEL STRUCTURE AND FINE-PARTICLE PRODUCTION METHOD USING THE SAME - A microchannel structure including a dispersed-phase introduction channel which communicates with a dispersed-phase introduction inlet; a continuous-phase introduction channel which communicates with a continuous-phase introduction inlet; a discharge channel which communicates with a discharge outlet; a fine-particle formation channel; and a plurality of branch channels for dispersed-phase introduction which are microchannels; wherein one end of the fine-particle formation channel in a fluid traveling direction communicates with the continuous-phase introduction channel whereas the other end thereof communicates with the discharge channel; and wherein a side part of the dispersed-phase introduction channel and side part of the fine-particle formation channel communicate via the plurality of branch channel for dispersed-phase introduction. | 09-18-2008 |
| 20090078574 | CHARGE TAGS AND THE SEPARATION OF NUCLEIC ACID MOLECULES - The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials. | 03-26-2009 |
| 20080202932 | Electrophoresis device and electrophoresis method - To reduce the elongation of the migration time for the first electrophoresis cycle in successive electrophoresis cycles and improve the reliability of electrophoresis analysis. The present invention relates to making the condition of a migration medium at the start of the first electrophoresis analysis in successive electrophoresis analyses after the temperature in a thermostatic oven reaches a desired preset temperature substantially the same as the conditions after the successive electrophoresis analyses. Preferably, a voltage is applied to a separation medium filling a capillary during preheating of the thermostatic oven. Preferably, the temperature in the thermostatic oven during preheating is set higher than the temperature in the thermostatic oven during electrophoresis analysis. Preferably, a buffer solution is heated during preheating of the thermostatic oven. Preferably, the capillary is filled with a preheated separation medium during preheating of the thermostatic oven. According to the present invention, the reliability of the first electrophoresis analysis in successive electrophoresis analyses is improved. | 08-28-2008 |
