BUDDHIST TZU CHI MEDICAL FOUNDATION Patent applications |
Patent application number | Title | Published |
20160095886 | COMPOSITION FOR TREATMENT OF JOINT DISEASE AND METHOD THEREOF - The present invention provides a pharmaceutical composition comprising mesenchymal stem cells, a hyaluronan and a pharmaceutically acceptable carrier. The pharmaceutical composition is used for the treatment of the joint disease. The mesenchymal stem cells in the composition are at least one selected from the group consisting of infra-patellar fat pad stromal cells, bone marrow stem cells, Wharton's jelly stem cells and adipose derived stem cells. The concentration of the hyaluronan is in a range from 25% (v/v) to 75% (v/v). The present invention further provides a method for treating a joint disease, comprising a step of administrating a composition containing mesenchymal stem cells, a hyaluronan and a pharmaceutically acceptable carrier to an injured joint tissue of a subject. | 04-07-2016 |
20150240210 | METHOD FOR RAPID MINCING OF ADIPOSE TISSUES TO ISOLATE LIVE CELLS IN VITRO - A method for rapid mincing of adipose tissues to isolate live cells in vitro is disclosed to overcome the drawbacks of a lead procedure of isolating live cells by holding a knife to mince adipose tissues, which can only isolate a small number of live cells and obtain low cell viability. The method includes: providing an adipose tissue; mincing the adipose tissue homogeneously by a mincing device; adding a reagent into the minced adipose tissue to perform hydrolysis; performing centrifuge and isolation; and removing a supernatant to obtain a cell pellet. Therefore, the time of mincing adipose tissues can be shortened, and contaminations caused by repeated use of the knife can be avoided. The method can be used for isolating live cells of adipose tissues to improve the number of live cells per unit weight of adipose tissues without reducing the cell viability. | 08-27-2015 |
20150159137 | ADJUVANT FOR RAPID PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO, METHOD FOR RAPID PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO, METHOD FOR GROWTH FACTOR HARVESTED FROM RAPID PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO AND USE THEREOF - An adjuvant for rapid proliferation of human mesenchymal stem cells in vitro is provided to overcome the problem of low cell amplification efficiency of human mesenchymal stem cells in a culture process. The adjuvant added for the culture of human mesenchymal stem cells includes at least one antioxidant, and a basic fibroblast growth factor (FGF-2). The adjuvant is added into a medium containing the human mesenchymal stem cells, and the culture takes place in a normal oxygen environment (21% oxygen tension), and the cells are divided rapidly, and the cell cycle at synthesis phase (S phase) percentage is increased to reduce ageing and improve differentiation potential. The adjuvant not only amplifies human mesenchymal stem cells rapidly to harvest the growth factor, but also maintains the characteristics of the multifunction of stem cells for the purposes of culturing and amplifying the human mesenchymal stem cells. | 06-11-2015 |