20120088254 | WHOLE BLOOD ASSAY OF AN INTRACELLULAR BIOMARKER OF A CELL SIGNALLING PATHWAY USE IN MEASURING THE ACTIVATION OF A PREDETERMINED CELL POPULATION - A method for assaying by ELISA, carried out on a whole blood sample, of the cellular activation state of a predetermined population of cells of the sample, includes determining the phosphorylation state of an intracellular protein involved in a cell signaling pathway (biomarker). A particular application of the method for assaying the phosphorylation state of an intracellular protein involves monitoring agonist agents or antagonist agents of cellular activation of cellular populations present in a whole blood sample. Such an application may in particular contribute to therapeutic monitoring of patients, or it may be useful in drug screening. In particular, the application pertains to the context of the exploration of hemostasis, in particular blood coagulation, from an observation of the activation of platelets contained in whole blood samples. | 04-12-2012 |